KX-21

Training

Main Menu KX-21

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KX-21 Introduction
Training
Specifications
Main Menu
Features & Advantages
Measurement Principles
Sample Processing
Sample Rotor Valve
Histograms
Maintenance
Comparison KX-21/K-1000
Competition
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KX-21
Training
Advances through Innovation

Main Menu SE-AMS

SE-9000

NE-8000 SF-3000

K-4500
E-5000
M-2000 KX-21
K-
CC-800 1000
CC-780
PL-100 F-820
CC-180 F-800
CC-120
CC-107
CC-1001

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KX-21
Training

Main Menu

Introduction

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KX-21
Training
Introduction

.
Main Menu

proven technology offers easiest handling

and outstanding precision

.. small footprint
little sample volume

. highly precise measurement even in

extreme pathological samples

. multiple applications in routine and

emergency

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KX-21
Training
Introduction

..
Main Menu

easy handling
automatic system-check during start-up

.. optimized pre-diluted mode

automatic rinsing of the sample pipette

. little maintenance needed

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KX-21
Training
Introduction

Main Menu
Display
. LCD-display with user-
friendly menu structure

. in case of error-messages
automatical switch into the
HELP-program

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KX-21
Training
Introduction

Main Menu
Main Menu

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KX-21 Introduction
Training

Main Menu
Stored Data

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KX-21
Training
Introduction

Main Menu 3-Part-Differential of the Leukocytes

Discriminators are
manually
adjustable

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KX-21
Training

Main Menu

Specifications

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KX-21
Specifications
Training Parameters
Main Menu

 18 parameters:

WBC, RBC, HGB, HCT, PLT

MCV, MCH, MCHC
RDW-CV, RDW-SD, MPV, PDW, P-LCR
SCR% (LYMPH%), MCR% (MONO/EO/BASO%), LCR% (NEUT%)
SCC# (LYMPH#), MCC# (MONO/EO/BASO#), LCC# (NEUT#)

 3 histograms:

WBC, RBC, PLT

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KX-21
Specifications
Training Functionality
Main Menu

.. clear LCD-display
HELP - program available

.. simplified reagent management

minimised maintenance

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KX-21
Specifications
Training Sample Volumes
Main Menu

. Whole Blood Mode 50 µl

. Pre-diluted Mode 20 µl

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KX-21
Specifications
Training Reagents
Main Menu

. Cellpack

. Stromatolyser-WH
 Only one reagent for Leukocyte- and
Hemoglobin measurement
Alarm in case of use of non-Sysmex reagents!

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KX-21
Specifications
Training Reagent Consumption
Main Menu

Volume / Volume / Samples /

Container Sample Container

Cellpack 20 L 34 ml 580

Stromatolyser-WH 0,5 L 1,0 ml 470

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KX-21
Specifications
Training Measurement Principles
Main Menu

WBC/RBC/PLT DC Detection Method

HGB cyanide-free measurement

with quaternary Ammonium-
salts

HCT cumulative Pulse Height

Detection Method

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KX-21
Specifications
Training Throughput
Main Menu

Throughput
• 60 samples / hour

Cycle time
• 60 seconds / sample

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KX-21
Specifications
Training Quality Control
Main Menu

.. 1 X bar
6 QC files for control blood
...
20 parameters
60 data points
L-J charts

. Eightcheck-3WP

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KX-21
Specifications
Training Overview
Parameters WBC, NEUT%, LYMPH%, EO/MONO/BASO%,
Main Menu NEUT#, LYMPH#, EO/MONO/BASO#, RBC, HCT,
HGB, MCV, MCH, MCHC, RDW-SD, RDW-CV, PLT,
PDW, MPV, P-LCR

Reagents Diluent CELLPACK

Lyse-Reagent STROMATOLYSER-WH
Hemoglobin-Reagent STROMATOLYSER-WH
Detergent CELLCLEAN

Capillary-diameter WBC 100 µm

RBC/HCT/PLT 75 µm

Throughput 60 samples / hour

(ca. 60 seconds cycle time / sample)

Measurement principle - DC Detection Method (RBC, WBC, PLT)

- cyanide-free HBG-method
- cumulative Pulse Height Detection Method (HCT)
- calculation form RBC and HCT (MCV)
- calculation from RBC and HGB (MCH)
- calculation from HCT and HGB (MCHC)

Sample-volumes Whole Blood Mode 50 µl

Pre-diluted Mode 20 µl

Stored Data internal stored data for 240 samples (without histogram)
external stored data in K-DPS available

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Specifications
Training Overview
Precision A fresh, normal whole blood sample was analysed 10 times
Main Menu consecutively in Sampler Mode and the Coefficient of Variation
(CV) was calculated.

WBC CV < 3,5%

RBC CV < 2,0%
HGB CV < 1,5%
HCT CV < 2,0%
MCV CV < 2,0%
MCH CV < 2,0%
MCHC CV < 2,0%
PLT CV < 6,0%

Accuracy WBC ± 3,0%

RBC ± 2,0%
PLT ± 5,0%

Linearity WBC within ± 0,3 x 103/µl for 1,0 - 9,9 x 103/µl

or 3,0 % for 10,0 - 99,9 x 103/µl
RBC within ± 0,03 x 106/µl for 0,30 - 0,99 x 106/µl
or 3,0 % for 1,0 - 7,0 x 106/µl
HGB within ± 0,2 g/dl for 0,1 - 10,0 g/dl
and 2,0 % for 10,0 - 25,0 g/dl
HCT within ± 1,0 % for 10,0 - 33,3 %
or 3,0 % for 33,4 - 60,0 %
PLT within ± 10 X 10^3/µl for 10 - 199 x 103/µl
or 5,0 % for 200-999 x 103/µl
(if RBC < 7,00 x 106/µl

Blanks WBC < 0,3 x 103/µl

RBC < 0,02 x 106/µl
HBGG < 0,1 g/dl SYSMEX EUROPE GMBH
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KX-21
Specifications
Training Overview
Main Menu Display range WBC 0,00-299,9 x103/µl
RBC 0,00-19,99 x 106/µl
HGB 0,0-25,0 g/dl
HCT 0,0-100 %
PLT 0-1999 x 103/µl

Peripheral Interfaces serial port for host computer or K-DPS

Dimensions 480 x 420 x 355

WxHxD
(mm)

Power Supply 230 V AC with 50 Hz

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Training

Main Menu

Features & Advantages

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Training
Features & Advantages

. 
Main Menu

compact design small footprint

. 60 samples/ hour
 for medium
throughputs

. little sample-
volume
 paediatrics,
emergency labs

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KX-21
Training
Features & Advantages

.
Main Menu

LCD-display
 clear information

. automatic system
check
 safety

. HELP-program

 uncomplicated
trouble shooting

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KX-21
Training
Features & Advantages

Main Menu

. hot keys


easy handling

.
fast access
flag-system
 helpful support

. little maintenance
 time saving

. maintenance intervals
are displayed  work simplifying

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KX-21
Training
Features & Advantages

. 
Main Menu

absolute counting no calibration

needed

. LCD-display


low radiation
no ageing

. non-toxic reagent
system  environment
protection

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KX-21
Training

Main Menu

Measurement Principles

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Training
DC Detection Method

Main Menu From pulse to histogram

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DC Detection Method
Training Aperture
Main Menu
resistance

direct current vacuum

(approx. 100 V)

external electrode internal electrode

orifice

blood cells sample

sample beaker

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DC Detection Method
Training Aperture
orifice
Main Menu
external internal
electrode electrode

vacuum
U=RxI

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KX-21
DC Detection Method
Training Absolute Counting
Main Menu

250 µl 500 µl

Diaphragm-
pumps

RBC counting chamber WBC counting chamber

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DC Detection Method
Training Noise-Control
Main Menu Noise-control (without noise)

pulse height

pulse number
t1= 0,5 sec = t2= 0,5 sec = t3= 0,5 sec
pulse number t1 pulse number t2 pulse number t3

Noise-control (with noise)

pulse height

pulse number
t1= 0,5 sec t2= 0,5 sec t3= 0,5 sec
pulse number t1= =
pulse number t2 pulse number t3

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KX-21
DC Detection Method
Training Pulse Diagram
Main Menu From pulse to histogram

time
1 2 3 4 5 6 7 8 9 10 11 12 13 14

pulse height

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KX-21
Training
DC Detection Method
1 2 3 4 5 6 7 8 9 1011 1213 14

time
Main Menu

Pulse Diagram
(30 cells)

pulse height
1 2 3 4 5 6 7 8 9 1011 1213 14
30

Cumulative
20
Distribution Curve

10

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4 1 0 0 0 1 2 3 4 5 4 3 2 1
KX-21
Training
DC Detection Method
1 2 3 4 5 6 7 8 9 1011 1213 14
30
Main Menu

Cumulative
20
Distribution Curve

10

1 2 3 4 5 6 7 8 9 1011 1213 14

4 1 0 0 0 1 2 3 4 5 4 3 2 1

Histogram

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KX-21
DC Detection Method
Training Histogram
Main Menu From pulse to histogram
Cumulative Distribution Curve
3000

Maximum of the histogram

2500

Histogram 2000

1500

Turning point of the

cumulative distribution 1000
curve
500

0
0 6 12 18 24 30 36 42 48 54 60 66 72 78 84 90 96 102108114120

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KX-21
DC Detection Method
Training Absolute Counting
defined aperture
volume sample
Main Menu
vacuum

stop start
signal-pulses

noise-pulses

discriminator

signal-pulses
off on
 Not to be changed by the user:
counter sample volume;
dilution ratio;
analysed volume;
diameter of the orifice
display:
 The instrument automatically checks:
S pulse / volume counting time, condition of the orifice
during the analysis (noise-control)
 Advantages:
no calibration of the counts,
pulse management digital automatical check of the orifice
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KX-21
DC Detection Method
Training Relative Counting
sample aperture
Main Menu vacuum

reference-sample
for system -
calibration
signal-pulses

noise-pulses

discriminator
Rated value of
reference-sample signal-pulses
 Counting of particles per time unit
 Unsafety:

pulses /time
reference sample;
electrical strain condition of the orifice;
digital-analog changer vacuum
Adjustment of
the strain to pulse management digital
the rated value
of the ref.
sample pulse management analog
analog-digital changer
(digital-Voltmeter)

display:
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(= rated value of the Ref.-sample)
KX-21
DC Detection Methods
Training Comparison
Main Menu

Absolute Counting Relative Counting

 Counting of particles in a  Counting of particles per time
defined volume unit

 Cell concentration is The counting rate is determined

determined by a known sample indirectly by a reference sample. A
volume and a known dilution similar behaviour of sample and
calibrator - resulting in comparable
results - is assumed.

 No calibration of the counting  Calibration of the counting results

results needed is mandatory.

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KX-21
DC Detection Method
Training Erythrocyte-Histogram
Main Menu

25-75 fl 200-250 fl

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KX-21
DC Detection Method
Training Platelet-Histogram
Main Menu

fixed at
2-6 fl 12 fl 12-30 fl

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KX-21
Training

Main Menu

Analysis of the Hemoglobin

Concentration

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KX-21
Training
Hemoglobin

Main
Main Menu
Menu

.. Hemoglobin is the red blood dye.

Chemically, Hemoglobin is an iron-containing
protein.

. It is produced in the Erythroblasts of the bone

marrow.

.. Hemoglobin is present in the Erythrocytes.

The Hemoglobin concentration is directly linked to
the Hct and the Erythrocyte-concentration.

. Hemoglobin is responsible for transporting oxygen

and CO2.

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KX-21
Hemoglobin
Training Reference Ranges
Main
Main Menu
Menu

New borns 14 - 26 g/dl

Children 10 - 26 g/dl
Women 12 - 16 g/dl
25
Men 14 - 18 g/dl
20

15

10

0
New borns Babies Children Women Men

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KX-21
Hemoglobin
Training Cyanide-free method
Main
Main Menu
Menu

. Stromatolyser-WH is used for the Hemoglobin-

and the leukocyte-analysis.

. Stromatolyser-WH contains quaternary

Ammonium salts.

. There are a very good correlations between the

Sysmex-SLS-Method and the reference method.

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Hemoglobin
Training Reagent Composition Reagent
Main
Main Menu
Menu

. Stromatolyser WH contains the following

quaternary Ammonium salts:

. MTAC Myristyltrimethylammoniumchloride

. LTAC Lauryltrimethylammoniumchloride

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Hemoglobin
Training 1. Lysis of the Erythrocytes
Main
Main Menu
Menu

Hemoglobin-molecule

Fe2+  Fe2+ Fe2+  Fe2+

MTAC +
 LTAC 

RBC RBC

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Hemoglobin
Training 2. Change in the conformity
Main Menu

Hemoglobin-molecule

Fe2+  Fe2+ Fe2+  Fe2+

 

RBC RBC

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Hemoglobin
Training 3. Oxidation
Main Menu

Hemoglobin-molecule
O2

Fe2+  Fe2+ Fe3+  Fe3+

 

Methemoglobin-complex

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Hemoglobin
Training Spectral-analysis
Main Menu

Absorption STROMATOLYSER-WH

SULFOLYSER

540 630 Wave length (nm)

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Hemoglobin
Training Method following DIN 58931
Main Menu

.. Erythrocytes are lysed in a dilution of 1:500.

1. reaction step:
HBG with the bound Fe2+ is oxidised by the Potassium
hexocyanoferrate (K3(Fe(CN)6) to become Methemoglobin
(with Fe3+).

. 2. reaction step:
Methemoglobin reacts with Potassium cyanide (KCN),
building the red and stable Cyan hemoglobin-complex with
an maximum in absorption at 546 nm.

. The extinction at the maximum of absorption is directly

proportional to the Hemoglobin content of the whole blood.

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Hemoglobin
Training Modified Cyan methemoglobin Method
Main Menu

. Identical measurement principle as the reference

method.

. Measurement in the photometer under the same

conditions.

.. Also the reaction conditions are identical.

Cyanide containing reagent for haemolysis.
Seem automated systems = Quicklyser
Fully automated systems= Stromatolyser-C

. Disposal of cyanide containing waste needs a

permission.

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Hemoglobin
Training HGB-Photometer/Cyanide free Method
Main Menu

. photometrical analysis at 555 nm

Sample stream Cellpack

LED lens Flow cell photosensor

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Hemoglobin
Training Flow Chart Flow Chart
Main Menu

Start

HGB-chamber is rinsed with diluent.

HGB-blank is determined and stored.

Measurement of the sample

in a dilution of 1:500.

Sample - Blank = HGB-result.

Printing of the result.

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End
KX-21
Training

Main Menu

Hematocrit Determination

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Training
Hematocrit

Main Menu

. Hematocrit is the relative volume-proportion of the

red blood cells in the total blood.

. Determination by centrifugation or calculation.

. Besides Hemoglobin, Hematocrit is a measure for

anaemia.

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Hematocrit
Training Reference Ranges
Main Menu

New borns 0,51 - 0,65

Children 0,35 - 0,43
Women 0,38 - 0,48

Men 0,42 - 0,52

0,5

0,4

0,3

0,2

0,1

0
New borns Babies Children Women Men

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Hematocrit
Training Centrifugation
Main Menu

. Simple volume determination of the corpuscular

particles in the blood.

.. Normally, equivalent to the pure erythrocyte-mass.

Blood is aspirated is special capillaries, which contain
dried heparin.

. The capillaries are sealed with a special folio and put

into a dedicated centrifuge.

. In the high-turning centrifuge the non-coagulating blood

is separated into its solid and fluid parts using the
different specific weights of plasma and blood particles.

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Hematocrit
Training Centrifugation
Main Menu

Total volume VT

Centrifuge

VT
HCT (%) = V / VT x100

60
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KX-21
Hematocrit
Training Centrifugation
Main Menu

. The reading is done on a scale, which is based

on the principle of similar triangles.

. The guide, which cuts through the

border between erythrocyte- 1,0
and leukocyte-layer gives 0,9
the Hematocrit-value. 0,8
Leukocytes 0,7
(Buffy Coat) 0,6
Erythrocytes 0,5
0,4
0,3
0,2
0,1
0
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Hematocrit
Training Cum. Pulse Height Detection Method
Main Menu

Transducer

Start-Sensor
Defined volume VT
Stop-Sensor

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KX-21
Hematocrit
Training Cum. Pulse Height Detection Method
Main Menu

Ph = k x VEry
VEry = 1 / k x Ph

Ph Pulse height
k Constant
Ph VEry Volume of one RBC

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KX-21
Hematocrit
Training Cum. Pulse Height Detection Method
Main Menu

Ph = k x VEry
VEry = 1 / k x Ph

VT HCT (%) = V / VT x 100

V =  VEry

V = 1 / k  Ph

HCT (%) = 1 / VT k  Ph x 100

Ph Pulse height
k Constant
VEry Volume of one RBC
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KX-21
Hematocrit
Training Cum. Pulse Height Detection Method
Main Menu

VT
Ph

Transducer Ph = k x VEry
HCT (%) = V / VT x 100
VEry = 1 / k x Ph
V=  VEry = 1 / k  Ph
Ph Pulse height
k Constant
HCT (%) = 1 / VTk  Ph x
VEry Volume of one RBC
100
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KX-21
Training

Main Menu

Erythrocyte Quotients

Biological Variation

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KX-21
Training
RBC-Quotients

Main Menu

Mean Cell Volume (MCV)

HCT (%)
MCV (fl) =
RBC (x 106/µl)

Mean Cellular Hemoglobin (MCH)

HBG (g/dl)
MCH (pg) =
RBC (x 106/µl)

Mean Cellular Hemoglobin Concentration

(MCHC)
HBG (g/dl)
MCHC (g/dl) =
HCT (%)
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KX-21
Biological Variation
Training of CBC-parameters
Main Menu
Deviation from median in %
100
WBC
PLT n = 411 , confidence range 2,5 % to 97,5 %
80 taken from : R. Haeckel, Rationalisierung des med.
Laboratoriums, 2. Auflage GIT Verlag, Darmstadt

60

40

HGB RBC HCT

20
MCV MCH
MCHC
0 5,0 190 14 4,5 42 90 34 31

-20
103/µl 103/µl g/dl x 106/µl % fl g/dl pg
-40

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KX-21
Training

Main Menu

Sample Processing

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KX-21
Training

Main Menu

Sample Processing

Whole Blood Mode

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KX-21
Whole Blood Mode
Training Schematical Flow Chart
Main Menu
1,96
Cellpack ml RBC detection chamber
1,996 ml
40 µl 1:25.000
mixing
chamber
4 µl 1:500

1,994 ml
1,0 ml WBC detection chamber
Stromatolyser-WH 1:500

(PDV)
Sample Rotor Valve
6 µl

HBG
photometer
1:500

50 µl whole blood
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KX-21
Training

Main Menu

Sample Processing

Pre-diluted Mode

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Pre-diluted Mode
Training Schematical Flow Chart
Main Menu
2 ml
Cellpack RBC detection chamber
2,08 µl 1:25.000

1,922 ml
1,0 ml WBC detection chamber
Stromatolyser-WH 1:1000

(PDV)
Sample Rotor Valve
78 µl

HBG
photometer
1:1000

200 µl pre-diluted sample (1:26)

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KX-21
Training

Main Menu

Dilution Ratios

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Dilution Ratios
Training RBC/PLT: Whole Blood Mode
Main Menu

1. dilution
4 µl whole blood + 1,996 ml Cellpack
= 500 dilution factor
4 µl whole blood

2. dilution
40 µl 1. dil.. + 1,960 ml Cellpack
= 50 dilution factor
40 µl 1. dilution

Final dilution : 1. dilution x 2. dilution = 25.000

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KX-21
Dilution Ratios
Training RBC/PLT: Pre-diluted Mode
Main Menu

Pre-dilution
20 µl whole blood + 500 µl Cellpack
= 26 dilution factor
20 µl whole blood

1. dilution
2,08 µl pre-dil. + 1997,92 µl Cellpack
= 962 dilution factor
2,08 µl pre-dilution

Final dilution : pre-dilution. x 1. dilution = 25.000

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KX-21
Dilution Ratios
Training RBC/PLT: Counted cells / µl
Main Menu

whole blood mode:

5,00 x 106 cells / µl

x 250 µl = 50.000 cells
25.000 (dilution ratio)

pre-diluted mode:

5,00 x 106 cells / µl

x 250 µl = 50.000 cells
25.000 (dilution ratio)
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KX-21
Dilution Ratios
Training WBC: Whole Blood Mode
Main Menu

6 µl whole blood + 2,994 ml Str-WH

= 500 dilution factor
6 µl whole blood

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KX-21
Dilution Ratios
Training WBC: Pre-diluted Mode
Main Menu

Pre-dilution
20 µl whole blood + 500 µl Cellpack
= 26 dilution factor
20 µl whole blood

1. dilution
78 µl pre-dil. + 2,922 ml Str-WH
= 38,5 dilution factor
78 µl pre-dil.

Final dilution : pre-dilution x 1. dilution = 1.000

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KX-21
Dilution Ratios
Training WBC: Counted cells / µl
Main Menu

whole blood mode:

5.000 cells / µl
x 500 µl = 5.000 cells
500 (dilution ratio)

Pre-diluted mode:

5.000 cells / µl
x 500 µl = 2.500 cells
1.000 (dilution ratio)

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KX-21
Dilution Ratios
Training Comparison
Main Menu

RBC/PLT WBC
dilution factor counted dilution ratio counted
cells / µl cells / µl
whole blood mode
KX-21 25.000 50.000 500 5.000
K-1000/K-4500 25.000 50.000 250 8.000

pre-diluted mode
KX-21 25.000 50.000 1.000 2.500
K-1000/K-4500 25.000 50.000 1.250 1.600

Displayed cells: RBC 5 x 106 cells/µl

WBC 5.000 cells/µl SYSMEX EUROPE GMBH
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KX-21
Training

Main Menu

Sample Rotor Valve

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KX-21
Sample Rotor Valve
Training Construction
Main Menu

Upper part
(fixed)

Middle part
(movable)

Lower part
(fixed)

Aspiration pipette

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KX-21
Sample Rotor Valve
Training Construction
Main Menu

Upper part
(fixed)

Middle part
(movable)

Lower part
(fixed)

Aspiration pipette

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KX-21
Sample Rotor Valve
Training Construction
Main Menu

Upper part
(fixed)

Middle part
(movable)

Lower part
(fixed)

Aspiration pipette

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KX-21
Sample Rotor Valve
Training Aspiration of whole blood
Main Menu

Diaphragm-Pump

50 µl whole blood
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KX-21
Sample Rotor Valve
Training Sample Separation
Main Menu

6 µl (WBC) 4 µl (RBC)

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KX-21
Sample Rotor Valve
Training RBC-dilution
Main Menu

mixing chamber
4 µl (RBC)

diluent-
Diaphragm pump
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KX-21
Sample Rotor Valve
Training Aspiration for 2. RBC-dilution
Main Menu

mixing chamber

2. dilution
& Diaphragm pump
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KX-21
Sample Rotor Valve
Training Dosage for 2. RBC-dilution
Main Menu

40 µl

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KX-21
Sample Rotor Valve
Training 2. RBC-dilution
Main Menu

RBC-transducer-
chamber

diluent-Diaphragm pump
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KX-21
Sample Rotor Valve
Training WBC- and HGB- Sample-dilution
Main Menu

WBC-transducer
chamber
HGB-detector block

6µl (WBC)

diluent-Diagphragm pump
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KX-21
Training

Main Menu

Histograms

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KX-21
Histograms
Training Interpretation
NO. 4
Main Menu DATUM: 9/10/95
15:11
MODE: whole blood
WBC 5,8 x
103/µl
RBC 4,84
x106/µl
HGB 13,7 g/dl
HCT 42,0 %
MCV 86,8 fl
MCH 28,3 pg WBC
MCHC 32,6 g/dl
PLT 257
x103/µl 300
LYMPH% 31,2 %
MXD% 6,8 %
NEUT% 62,0 %
LYMPH# 1,8 x103/µl
MXD# 0,4 x103/µl
NEUT# 3,6 x103/µl

RBC

250
RDW-SD 40,0 fl

PLT

40
PDW 13,1 fl
MPV 10,4 fl
SYSMEX EUROPE GMBH
94 P-LCR 28,1 %
KX-21
Histograms
Training Normal haematological Report
Interpretation
NO. 4
Main Menu DATUM: 9/10/95
15:11
MODE: whole blood
WBC
103/µl
5,8 x CBC - parameter
RBC 4,84
x106/µl
HGB 13,7 g/dl
HCT 42,0 %
MCV 86,8 fl
MCH 28,3 pg WBC

WBC
MCHC 32,6 g/dl
PLT 257
300
Leukocyte-histogram
x103/µl
LYMPH% 31,2 %
MXD% 6,8 % Lymphocytes in % and absolute
NEUT% 62,0 % Eo, Mono, Baso in % and absolute
LYMPH# 1,8 x103/µl Neutrophiles in % and absolute
MXD# 0,4 x103/µl
NEUT# 3,6 x103/µl
Erythrocyte-histogram
RBC
RBC
Erythrocyte distribution width
250
RDW-SD 40,0 fl
Thrombocyte-histogram
PLT Thrombocyte distribution width
Mean Platelet Volume

PLT
40 Platelets - Large Cell Ratio
PDW 13,1 fl
MPV 10,4 fl
SYSMEX EUROPE GMBH
95 P-LCR 28,1 %
KX-21
Histograms
Training Reference Ranges of the red CBC
Main Menu

Histograms Parameter Sex Convent.Units SI-Units

RBC Men 4,6-6,2 x 106/µl x 1012/l
Women 4,2-5,4 x 106/µl x 1012/l

HGB Men 14-18 g/dl 8,5-11,0 mmol/l

Women 12-16 g/dl 7,5-10,0 mmol/l

HCT Men 43-49 % 0,43-0,49 mmol/l

Women 36-46 % 0,36-0,46 mmol/l

MCV 85-95 fl
MCH 27-33 pg 1,68-2,05 fmol
MCHC 32-36 g/dl 19,9-22,4 mmol/l

RDW-SD 37-46 fl (width in 20% peak height)

RDW-CV 11-16 % (calculated from width in 60 % peak height)

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KX-21
RBC- and PLT Histograms
Training Interpretation
Main Menu

Histograms

2 fl 25-30 fl 250 fl

• The distribution curve are separated by a moving discriminator.

• The thrombocytes range between 8 and 12 fl and are counted between 2
and 30 fl.
• Die erythrocytes range between 80 and 100 fl and are counted between
25 and 250 fl.
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KX-21
RBC- Histogram
Training Distribution Width
Main Menu

Histograms 100 % Turning Points

RDW-CV (%) = 100 x s/µ

s µ s
68,26 %
of all values

100 %

RDW-SD: 37 - 46 fl
20 %
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KX-21
RBC- Histogram
Training Interpretation
Main Menu

Histograms

Base line

2 fl 250 fl

• The distribution curve should always start on the base

line and lie within the discriminators.

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ RL “, abnormal height at the lower discriminator

LD
RBC

PLT

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ RL “, abnormal height at the lower discriminator

LD
RBC

Curve does not start at the base line.

PLT

Possible cause: CAUTION:

••Giant platelets
Micro-erythrocytes
All results flagged with “ RL “ should be checked!!

•Platelet aggregation

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ RU “, abnormal height at the upper discriminator

UD
RBC

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ RU “, abnormal height at the upper discriminator

UD
RBC

Curve does not end at the baseline.

Possible cause: CAUTION:

• Cold Agglutinins The RBC-result and all results flagged with “ RU “
• Erythroblasts should be checked!!
Normoblasts

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ MP “, Multiple peaks

RBC

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “ MP “, Multiple peaks

RBC

Possible cause:
• Transfusions, the patient’s and the donor’s RBCs most
probably have different sizes.

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “DW “, Distribution width

RBC

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KX-21
RBC- Histogram
Training Flagging
Main Menu

Histograms flag “DW “, Distribution width

RBC

• It is impossible to determine the distribution width, because

the histogram does not cut the 20%-line for a second time.
• This curve is only an example and might also show a
different course.

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KX-21
RBC- Histogram
Training Distribution Width
Main Menu

Histograms 100 % RDW-CV (%) = 100 x s/µ

Turning Points

s µ s RDW-CV
68,26 % 11 - 16 %
of all values
100 % The erythrocyte distribution
width is used as an indicator for
anisocytosis.

20 %

RDW-SD
37 - 46 fl
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KX-21
PLT- Histogram
Training Reference Ranges
Main Menu

Histograms Parameter Convent.Units SI-Units

PLT 150-350 x 10³/µl x 109/l

PDW 9-14 fl (Width in 20% peak height)

MPV 8-13 fl
P-LCR 15-35 %

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KX-21
PLT- Histogram
Training Interpretation
Main Menu

Histograms The curve is marked off by two

discriminators.

• The distribution curve should lie between the discriminators and start
and end at the base line.
• The measurement area is between 2 and 30 fl.

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KX-21
PLT- Histogram
Training Interpretation
Main Menu

Histograms • PLT, Platelet count LD UD

100 %

• Parameter from the thrombocyte-histogram PLT P-LCR

– MPV, Mean Platelet Volume between the
upper and the lower discriminator
Normal range: 8 - 12 fl
Use for control of erythropoiesis
20 %
– P-LCR, Platelet - Large Cell Ratio
Normal range: 15 - 35 % 12 fl
Elevation might be hint for : PDW
• Thrombocytes aggregates
• Micro erythrocytes
Pct (%)
– PDW, Platelet Distribution Width in 20 % MPV (fl) =
of total curve height PLT (x 103/µl)
Normal range: 9 - 14 fl
Elevation might be hint for:
• Thrombocytes aggregates
• Micro erythrocytes
• Erythrocyte fragments

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ PL “, abnormal height of the curve at the lower discriminator

PLT

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ PL “, abnormal height of the curve at the lower discriminator

PLT
PLT
Curve does not start at the base line.

Possible cause: CAUTION:

• Blank is too high PLT-result and all results flagged with “ PL “
• Cell fragment or cell ruins should be checked!
Thrombocytes also in the counting chamber,
if needed!

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ PU “, abnormal height of the curve at the upper discriminator

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ PU “, abnormal height of the curve at the upper discriminator

Curve does not end at the base line.

Possible cause: CAUTION:

• Coagulation of the blood
• Pseudo-thrombocytosis
• Micro erythrocytes
• Giant Platelets
• Thrombocyte aggregates
115
PLT-result and results flagged with “ PU “
should be checked!
Thrombocytes also in the counting chamber,
if needed!
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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ MP “, Multiple peaks

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ MP “, Multiple peaks

Possible cause:
• Transfusions, the patient’s and the donor’s PLTs most
probably have different sizes.

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ DW “, Distribution width

PLT

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KX-21
PLT- Histogram
Training Flagging
Main Menu

Histograms flag “ DW “, Distribution width

PLT

• The distribution curve should lie between the discriminators and start
and end at the base line.
• The measurement area is between 2 and 30 fl.

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KX-21
Histograms
Training Reference Ranges of the white CBC
Main Menu

Histograms Parameter Age range Convent.Units SI-Units

WBC grown ups 4-10 x 103/µl x 109/l
children up to 12 x 103/µl x 109/l
babies up to 15 x 103/µl x 109/l

W-SCR grown ups 25-40 %

children, babies up to 70 %
W-MCR grown ups 3-13 %
W-LCR grown ups 50-70 %

W-SCC grown ups 1-4 x 103/µl x 109/l

children up to 5 x 103/µl x 109/l
babies up to 6 x 103/µl x 109/l
W-MCC grown ups 0,2-1 x 103/µl x 109/l
W-LCC grown ups 2-7 x 103/µl x 109/l

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KX-21
WBC- Histogram
Training Influence of the lysing reagent
Main Menu

Histograms schematic picture after lysis

of a leukocyte

Electrolyte-solution
Mitochondrion

Nucleus

Nucleolus

Cell membrane

Ribosome

Cytoplasm

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KX-21
WBC- Histogram
Training Influence of the lysing reagent
Main Menu

Histograms before lysis

Cell diameter in µm
Neutrophiles 10 - 15
Basophiles 9 - 14
Eosinophiles 11 - 16
Monocytes 12 - 20
Lymphocytes 7 - 12
0 2 4 6 8 10 12 14 16 18 20 22

after lysis
Lymphocytes

Neutrophiles Cell volume in fl

Monocytes Lymphocytes 30 - 80
Basophiles Monocytes 60 - 120
Eosinophiles Basophiles 70 - 130
Eosinophiles 80 - 140
Neutrophiles 120 - 250
0 50 100 150 200 250 300
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KX-21
WBC- Histogram
Training Interpretation
Main Menu

Histograms Important : The curve is marked off by 2 discriminators.

• The distribution curve
should lie within the
discriminators; i.e. the curve
starts and ends at the base LD T1 T2 UD ( fixed)
line.
• The lower discriminator
(LD) is flexible ,but cannot
be set below 30 fl.
• In the WBC-channel
leukocytes and thrombocytes
are present (erythrocytes are 0 50 100 150 200 250 300
lysed).
• The volume of the
thrombocytes is normally
between 8 and 12 fl, i.e. the
LD in the WBC-histogram
separates leukocytes from
the thrombocytes and the SYSMEX EUROPE GMBH
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thrombocytes are not
counted.
KX-21
WBC-Histogram
Training Flagging
Main Menu

Histograms flag “ WL “, curve does not start at the base line

CAUTION:
WBC - results and all results flagged
“WL” should be checked
(counting chamber if needed)!

Possible cause:
• Thrombocyte aggregates, Pseudo-Thrombopenia,
sample coagulated
• elevated osmotic resistance
(erythrocytes are not lysed)
• Normoblasts / Erythroblasts SYSMEX EUROPE GMBH
• Cold Agglutinins
KX-21
WBC-Histogram
Training Flagging
Main Menu

Histograms flag “ WU “, curve does not end at the base line

CAUTION:
WBC - results and all results flagged
“WU” should be checked
(counting chamber if needed)!

Sample dilution? (high leukocytes value? )

Counting chamber?

SYSMEX EUROPE GMBH

KX-21
WBC-Histogram
Training Flagging
Main Menu

Histograms flags “T1” and “T2”

LD T1 T2 UD ( fixed)

F = fraction
population 3 = F3

0 50 100 150 200 250 300

population 1 = F1 population 2 = F2

T1 and T2 are so called troughs. The purpose of these discriminators is to separate

the leukocytes into 3 populations.
• The discriminators are flexible in certain areas to adapt to the individual
sample in most cases.
• In very special cases the troughs are not able to separate between the
populations properly.
SYSMEX EUROPE GMBH
KX-21
WBC-Histogram
Training Flagging
Main Menu

Histograms flags “T1” and “T2”

It is not possible CAUTION:

in this case to • If the sample is flagged with
set T1, because
T1 or T2, the results from
there was no
valley found.
the pre-differential should
In this case T1 not be reported.
is flagged. • The leukocytes-result is
reportable (if there is no
other flag). All leukocytes
are counted.
Here T1 was
found, but no
second
valley for
T2.
Therefor, T2
is flagged.
SYSMEX EUROPE GMBH
KX-21
WBC-Histogram
Training Flagging
Main Menu

Histograms flags “F1” , “F2” and “F3”

The distribution curve of the leukocytes

is marked off by the discriminators
LD and UD.

There is the possibility that the fractions

are mixed, i.e. F1 and F2 cannot be
F1 F2 F3 separated properly, the same is true
for F2 and F3.

• All leukocytes are counted; so the total number of leukocytes ( WBC ) is

correct. ( Condition: no other flags are present).
• Also the valleys have been found and T1 and T2 are flagged accordingly.
• But it is noticeable that the valleys are far from the base line.

SYSMEX EUROPE GMBH

KX-21
Training

Main Menu

Maintenance

SYSMEX EUROPE GMBH

KX-21
Maintenance
Training Power ON
Main Menu

1. External control

. Is there enough reagent for the routine?

CELLPACK ca. 580 Samples / 20 l
STROMATOLYSER-WH ca. 470 Samples / 0,5 l

. Is there sufficient paper in the printer?

. Are tubing and cables properly connected?

SYSMEX EUROPE GMBH

KX-21
Maintenance
Training Power ON
Main Menu

2. Turn KX-21 on
Turn KX-21 with the power switch on (right instrument side).
3. An automatical rinse is performed.
Acceptable blanks:
WBC ± 0,3 x 103/µl
RBC ± 0,02 x 106/µl
HGB ± 0,1 g/dl
PLT ± 10 x 103/µl
4. KX-21 turns into READY.
After a pre-defined time period the system turns into Standby.
5. Perform the quality control.

SYSMEX EUROPE GMBH

KX-21
Maintenance
Training Power OFF
Main Menu

Switching Off

 Press the button Shutdown.

 Let KX-21 aspirate CELLCLEAN. After the double

beep remove the CELLCLEAN. The rinse sequence is
performed automatically.

 Switch off KX-21.

SYSMEX EUROPE GMBH

KX-21
Maintenance
Training Intervals
Main Menu

Daily:


Clean transducer chambers and tubing (Shutdown)
Check trap chamber and empty if needed.

Weekly:
 Clean tray below the Sample Rotor Valve.

Monthly (or every 2500 samples):



Clean the waste chamber.
Clean WBC/RBC-transducer.

SYSMEX EUROPE GMBH

KX-21
Maintenance
Training Intervals
Main Menu

Quarterly (or every 7500 samples):

. Clean the Sample Rotor Valve.

As needed:
..Automatical rinsing sequence
Clean rinsing unit

.Clog removal
Program: ”Clog removal"

 Remove persisting clogs in the orifice

Program: ”Empty transducer chamber"
Cleaning of the orifice with dedicated brush
Document the maintenance procedure after the performance !!

SYSMEX EUROPE GMBH

KX-21
Training

Main Menu

Comparison KX-21 - K-1000

SYSMEX EUROPE GMBH

 KX-21
Training
Comparison

Main Menu
K-1000 KX-21
Measurement Principles DC Detection Method DC Detection Method
(WBC, RBC, PLT) (WBC, RBC, PLT)
SLS-Hb-Method (HGB) Cyanide-free Hb Method (HGB)
Cum. Pulse Height Detection Method Cum. Pulse Height Detection Method
Parameter 18 18
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR W-SCR, W-MCR, W-LCR
W-SCC, W-MCC, W-LCC W-SCC, W-MCC, W-LCC
RDW-CV or RDW-SD RDW-CV or RDW-SD
PDW, MPV, P-LCR PDW, MPV, P-LCR
Throughput
Whole Blood Mode 80 samples / hour 60 samples / hour
Pre-diluted Mode 60 samples / hour 60 samples / hour
Cycle Time
Whole Blood Mode 45 seconds 60 seconds
Pre-diluted Mode 60 seconds 60 seconds
Sample Volume
Whole Blood Mode 100 µl 50 µl
Pre-diluted Mode 40 µl 20 µl
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 KX-21
Training
Comparison

Main Menu
K-1000 KX-21
Precision [ % ] Whole Blood Capillary Blood Whole Blood Capillary Blood
WBC  3,0  9,0  3,5  6,0
RBC  1,5  4,5 2,0  3,0
HGB  1,5  4,5  1,5  2,5
HCT  1,5  4,5  2,0  3,0
MCV  1,5  4,5  2,0  3,0
MCH  1,5  4,5  2,0  3,0
MCHC  1,5  4,5  2,0  3,0
PLT  4,0  12,0  6,0  9,0

Linearity [%] [%]

(Whole Blood Mode)
WBC  3,0 6,7 - 99,9 x 103/µl  3,0 10,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 9,99 x106/µl  3,0 1,0 - 7,0 x 106/µl
HGB  1,0 10,1 - 20,0 g/dl  2,0 10,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 %  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl  5,0 200 - 999 x 103/

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 KX-21
Training
Comparison

Main Menu
K-1000 KX-21
Data Storage No 240 samples without histogram

Interfaces 1 serial 1 serial

1 parallel
Dimensions [ mm ] Main Unit Main Unit with integrated
Pneumatic Unit
480 x 555 x 298 420 x 480 x 355
Pneumatic Unit
195 x 295 x 395
Weight
Main Unit 28 kg Main Unit with integrated
Pneumatic Unit 14 kg Pneumatic Unit 30 kg
Total 42 kg
Reagent Consumption Cellpack 28 ml Cellpack 34 ml
Stromatolyser 3WP 1 ml Stromatolyser WH 1 ml
Sulfolyser 1 ml
Total 30 ml Total 35 ml

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KX-21
Training

Main Menu

Competition

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KX-21
Training
Competition

Main Menu

Abbott CD 1600
Coulter AcT 8
Hoffmann-La Roche Cobas-
Minos STX
ABX Micros
Dr. Lange LC 90
Becton-DickensonQB II
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 KX-21
Competition
Training CD 1600
Main Menu

Competition
K-X-21 CD 1600
Manufacturer TOA MEDICAL ELECTRONICS Abbott
Measurement Principles DC Detection Method DC Detection Method
(WBC, RBC, PLT) (WBC, RBC, PLT)
SLS-Hb-Method (HGB) Cyan methemoglobin-Method (HGB)
Cum. Pulse Height Detection Method
Parameter 18 18
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR GRAN (%, #), LYMPH (%,#), MID (%,#)
W-SCC, W-MCC, W-LCC
RDW-CV or RDW-SD RDW
PDW, MPV, P-LCR PDW, MPV, MPC
Throughput 60 samples / hour 60 samples / hour
Cycle Time 60 seconds 60 seconds
Sample Volume
Whole Blood Mode 50 µl 30 µl
Pre-diluted Mode 20 µl 40 µl

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 KX-21
Competition
Training CD 1600
Main Menu

Competition
KX-21 CD 1600
Precision [ % ] Whole Blood Capillary Blood Range
WBC  3,5  6,0 2 4,6 - 10,2 x 103/µl
RBC  2,0  3,0 1 4,0 - 6,1 x 106/µl
HGB  1,5  2,5 1 12,2 - 18,1 g/dl
HCT  2,0  3,0
MCV  2,0  3,0
MCH  2,0  3,0
MCHC  2,0  3,0
PLT  6,0  9,0 5 142 - 424 x 103/µl

Linearity [%] [%]

(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl 1,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl 1,0 - 7,0 x 106/µl
HGB  1,0 10,0 - 25,0 g/dl 2,5 - 24 g/dl
HCT  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl 10 - 999 x 103/

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 KX-21
Competition
Training CD 1600
Main Menu

Competition
KX-21 CD 1600
Data Storage 240 samples without histogram 320 samples

Interfaces 1 serial 1 serial

1 parallel (option)
Dimensions [ mm ] Main Unit with integrated Main Unit with integrated
Pneumatic Unit Pneumatic Unit
420 x 480 x 355 840 x 460 x 510

Weight Main Unit with integrated Main Unit with integrated

Pneumatic Unit 30 kg Pneumatic Unit 66 kg

Reagent Consumption Cellpack 34 ml

Stromatolyser WH 1 ml
Total 35 ml

Reagents environmentally Yes No, cyanide containing

save

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 KX-21
Competition
Training AcT 8
Main Menu

Competition
K-X-21 AcT 8
Manufacturer TOA MEDICAL ELECTRONICS Coulter (USA)
Measurement Principles DC Detection Method Impedance Measurement
(WBC, RBC, PLT) (WBC, RBC, PLT)
SLS-Hb-Method (HGB) Cyan methemoglobin-Method (HGB)
Cum. Pulse Height Detection Method
Parameter 18 8
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR
W-SCC, W-MCC, W-LCC
RDW-CV or RDW-SD
PDW, MPV, P-LCR
Throughput 60 samples / hour 55 samples / hour
Cycle Time 60 seconds 60 seconds
Sample Volume
Whole Blood Mode 50 µl 12 µl
Pre-diluted Mode 20 µl 20 µl

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 KX-21
Competition
Training AcT 8
Main Menu

Competition
KX-21 AcT 8
Precision [ % ] Whole Blood Capillary Blood Range
WBC  3,5  6,0 3,0 6,0 - 15,0 x 103/µl
RBC  2,0  3,0 3,0 3,0 - 6,0 x 106/µl
HGB  1,5  2,5 2,0
HCT  2,0  3,0
MCV  2,0  3,0 3,0 80 - 100 fl
MCH  2,0  3,0
MCHC  2,0  3,0
PLT  6,0  9,0 7,0 200 - 500 x 103/µl

Linearity [%] [%]

(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl 3,0 0,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl 2,0 0,0 - 7,0 x 106/µl
HGB  1,0 10,0 - 25,0 g/dl 2,0 0,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl 7,0 0,0 - 999,9 x 103/

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 KX-21
Competition
Training AcT 8
Main Menu

Competition
KX-21 AcT 8
Data Storage 240 samples without histogram ?

Interfaces 1 serial 1 serial

with ASTM standard
Dimensions [ mm ] Main Unit with integrated Main Unit with integrated
Pneumatic Unit Pneumatic Unit
420 x 480 x 355 375 x 420 x 370

Weight Main Unit with integrated Main Unit with integrated

Pneumatic Unit 30 kg Pneumatic Unit 11,5 kg

Reagent Consumption Cellpack 34 ml

Stromatolyser WH 1 ml
Total 35 ml Total 22 ml

Reagents environmentally Yes No, cyanide containing

save

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 KX-21
Competition
Training Cobas-Minos STX
Main Menu

Competition
K-X-21 Cobas-Minos STX
Manufacturer TOA MEDICAL ELECTRONICS Hoffmann-La Roche AG
Measurement Principles DC Detection Method Impedance Measurement
(WBC, RBC, PLT) (WBC, RBC, PLT)
SLS-Hb-Method (HGB) Cyan methemoglobin-Method (HGB)
Cum. Pulse Height Detection Method Cum. Pulse height Detection Method
Parameter 18 18
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR LY (%, #)
W-SCC, W-MCC, W-LCC MON / GRA (%,#)
RDW-CV or RDW-SD RDW
PDW, MPV, P-LCR MPV, PDW, PCT
Throughput 60 samples / hour 70 samples / hour
Cycle Time 60 seconds 60 seconds
Sample Volume
Whole Blood Mode 50 µl 25 µl
Pre-diluted Mode 20 µl

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 KX-21
Competition
Training Cobas-Minos STX
Main Menu

Competition
KX-21 Cobas-Minos STX
Precision [ % ] Whole Blood Capillary Blood Range
WBC  3,5  6,0 2 10 x 103/µl
RBC  2,0  3,0 2 5 x 106/µl
HGB  1,5  2,5 1 15 g/dl
HCT  2,0  3,0 2 45 %
MCV  2,0  3,0
MCH  2,0  3,0
MCHC  2,0  3,0
PLT  6,0  9,0 5 300 x 103/µl

Linearity [%] [%]

(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl 3,0 0,1 - 80,0 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl 2,0 0,5 - 8,0 x 106/µl
HGB  1,0 10,0 - 25,0 g/dl 2,0 2,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 % 3,0 10,0 - 70,0 %
PLT  5,0 200 - 999 x 103/µl 7,0 10 - 1000 x 103/µl

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 KX-21
Competition
Training Cobas-Minos STX
Main Menu

Competition
KX-21 Cobas-Minos STX
Data Storage 240 samples without histogram 300 samples

Interfaces 1 serial 1 serial

Dimensions [ mm ] Main Unit with integrated Main Unit 430 x 370 x 400
Pneumatic Unit Printer 370 x 130 x 440
420 x 480 x 355 Monitor 320 x 330 x 320

Weight Main Unit with integrated Main Unit 32 kg

Pneumatic Unit 30 kg Printer 10 kg
Monitor 13 kg
Reagent Consumption Cellpack 34 ml Mnoton 25 ml
Stromatolyser WH 1 ml Mnolyse 1 ml
Total 35 ml Total 26 ml

Reagents environmentally Yes No, cyanide containing

save CE2000, waste disposal system

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 KX-21
Competition
Training Micros
Main Menu

Competition
K-X-21 Micros
Manufacturer TOA MEDICAL ELECTRONICS ABX
Measurement Principles DC Detection Method
(WBC, RBC, PLT)
SLS-Hb-Method (HGB)
Cum. Pulse Height Detection Method
Parameter 18 18
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR W-SCR, W-MCR, W-LCR
W-SCC, W-MCC, W-LCC W-SCC, W-MCC, W-LCC
RDW-CV or RDW-SD RDW-CV or RDW-SD
PDW, MPV, P-LCR MPV, PDW, PCT
Throughput 60 samples / hour 40 samples / hour
Cycle Time 60 seconds 90 seconds
Sample Volume
Whole Blood Mode 50 µl 12 µl
Pre-diluted Mode 20 µl

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 KX-21
Competition
Training Micros
Main Menu

Competition
KX-21 Micros
Precision [ % ] Whole Blood Capillary Blood
WBC  3,5  6,0
RBC  2,0  3,0
HGB  1,5  2,5
HCT  2,0  3,0
MCV  2,0  3,0
MCH  2,0  3,0
MCHC  2,0  3,0
PLT  6,0  9,0

Linearity [%]
(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl
HGB  1,0 10,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl

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 KX-21
Competition
Training Micros
Main Menu

Competition
KX-21 Micros
Data Storage 240 samples without histogram option

Interfaces 1 serial 1 serial

Dimensions [ mm ] Main Unit with integrated Main Unit with integrated

Pneumatic Unit Pneumatic Unit
420 x 480 x 355 360 x 420 x 320

Weight Main Unit with integrated Main Unit with integrated

Pneumatic Unit 30 kg Pneumatic Unit 14 kg

Reagent Consumption Cellpack 34 ml

Stromatolyser WH 1 ml
Total 35 ml Total 22 ml

Reagents environmentally Yes Maybe toxical reagents??

save

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 KX-21
Competition
Training LC 90
Main Menu

Competition
KX-21 LC 90
Manufacturer TOA MEDICAL ELECTRONICS Dr. Lange
Measurement Principles DC Detection Method DC Detection Method
(WBC, RBC, PLT) (WBC, RBC, PLT)
SLS-Hb-Method (HGB) photometrical measurement of HGB
Cum. Pulse Height Detection Method at 540 nm wave length
Parameter 18 28
WBC, RBC, HGB, HCT WBC, RBC, HGB, HCT
MCV, MCH, MCHC, PLT MCV, MCH, MCHC, PLT
W-SCR, W-MCR, W-LCR LY (%,#), MO (%,#), GR (%,#)
W-SCC, W-MCC, W-LCC µRBC (%,#), macroRBC (%, #), EVB
RDW-CV or RDW-SD MPV, TKT, Mode PLT, Median PLT
PDW, MPV, P-LCR µPLT (%, #), macroPLT (%,#), PDW
Throughput 60 samples / hour 60 samples / hour
Cycle Time 60 seconds ?
Sample Volume
Whole Blood Mode 50 µl 30 µl
Pre-diluted Mode 20 µl 15 µl

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 KX-21
Competition
Training LC 90
Main Menu

Competition
KX-21 LC 90
Precision [ % ] Whole Blood Capillary Blood
WBC  3,5  6,0
RBC  2,0  3,0
HGB  1,5  2,5
HCT  2,0  3,0
MCV  2,0  3,0
MCH  2,0  3,0
MCHC  2,0  3,0
PLT  6,0  9,0

Linearity [%]
(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl
HGB  1,0 10,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl

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 KX-21
Competition
Training LC 90
Main Menu

Competition
KX-21 LC 90
Data Storage 240 samples without histogram 10.000 samples

Interfaces 1 serial 2 serial

1 parallel
Dimensions [ mm ] Main Unit with integrated
Pneumatic Unit
420 x 480 x 355

Weight Main Unit with integrated

Pneumatic Unit 30 kg

Reagent Consumption Cellpack 34 ml

Stromatolyser WH 1 ml
Total 35 ml

Reagents environmentally Yes

save

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 KX-21
Competition
Training QBC II
Main Menu

Competition
K-X-21 QBC II
Manufacturer TOA MEDICAL ELECTRONICS Becton-Dickinson
Measurement Principles DC Detection Method Centrifugation and buffy-coat
(WBC, RBC, PLT) determination (fluorescence and
SLS-Hb-Method (HGB) absorption of differentiated cell-layers
Cum. Pulse Height Detection Method HGB-calculation from density factors
Parameter 18 9
WBC, RBC, HGB, HCT WBC, HGB, HCT
MCV, MCH, MCHC, PLT MCHC, PLT
W-SCR, W-MCR, W-LCR Gran (% or #)
W-SCC, W-MCC, W-LCC Lymph / Mono (% or #)
RDW-CV or RDW-SD Fibrinogen
PDW, MPV, P-LCR
Throughput 60 samples / hour depending on user
Cycle Time 60 seconds 10 minutes in total
Sample Volume
Whole Blood Mode 50 µl 111 µl
Pre-diluted Mode 20 µl 55 - 65 µl

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 KX-21
Competition
Training QBC II
Main Menu

Competition
KX-21 QBC II
Precision [ % ] Whole Blood Capillary Blood Whole Blood Capillary Blood
WBC  3,5  6,0  7,0  8,0
RBC  2,0  3,0
HGB  1,5  2,5  1,5  1,5
HCT  2,0  3,0  2,0  2,0
MCV  2,0  3,0
MCH  2,0  3,0
MCHC  2,0  3,0  2,5  2,0
PLT  6,0  9,0  9,0  13,5

Linearity [%]
(Whole Blood Mode)
WBC  3,0 10,0 - 99,9 x 103/µl
RBC  3,0 1,0 - 7,0 x106/µl
HGB  1,0 10,0 - 25,0 g/dl
HCT  3,0 33,4 - 60,0 %
PLT  5,0 200 - 999 x 103/µl

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 KX-21
Competition
Training QBC II
Main Menu

Competition
KX-21 QBC II
Data Storage 240 samples without histogram ?

Interfaces 1 serial 1 serial

Dimensions [ mm ] Main Unit with integrated Autoread Analyser 343 x 102 x 241
Pneumatic Unit Centrifuge 235 x 127 x 298
420 x 480 x 355

Weight Main Unit with integrated Autoread Analyser 3,6 kg

Pneumatic Unit 30 kg Centrifuge 2,8 kg

Reagent Consumption Cellpack 34 ml dry hematology

Stromatolyser WH 1 ml (carrier driven reagents
Total 35 ml > capillaries)

Reagents environmentally Yes Yes, because no fluid reagents

save

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KX-21
Training
Thank you
for your
Main Menu

attention!

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