ABE LAB MANUAL

DOREEN OSGOOD, MS,PHD.

ABE RI COORDINATOR
CURRENT MANUAL

• Goals of the ABE curriculum:

– Engage a diverse population of students
– Deepen conceptual understandings of science
– Expand awareness about biotechnology and the nature of
scientific research
– Show the research and development process for biotechnology

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LAB MANUAL
• TAB A
– Amgen biotech Experience & Program introduction
– Chapter 1: Tools of the trade
• TAB B – Complete sequence
– Chapter 2: How do you begin to clone a gene?
– Chapter 3: Building a recombinant plasmid
– Chapter 4: Making sure you’ve got a recombinant
plasmid
– Chapter 5: Getting recombinant plasmids in bacteria
• TAB C – Abridged sequence
– All above EXCEPT Chapter 3
• TAB D
– Chapter 5B
• TAB E: Getting what we need

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 LAB MANUAL
ABE lab sequence:
• Complete genetic engineering sequence
New curriculum: – 3.5 weeks
Modular approach • Abridged genetic engineering sequence – “A”
– can use entire – 3 weeks
book or just • Focus on Bacteria sequence – “B”
individual chapters – 2 weeks
• Introduction to biotechnology
– About 6 days

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STUDENTS
STUDENTS

• Start at Tab A
– Everyone does
– About the Amgen Biotech Experience – important! (A-1)
• Please relay to students HOW they are getting to use this kit
– Then program introduction & tools of the trade (A-3 & A-15)

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STUDENTS
Click icon to add picture

• Program introduction (A-3)

– Very important!
– Shows real world relevance to a disease
process
– WHY should they care about this
curriculum?

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STUDENTS
• Each Chapter:
– Introduction
– Chapter goals
– What do you already know?
– Section purpose
– Before the lab
– Materials
– Methods
• Lab techniques
• Stop and think
• Safety
– Questions
– Glossary

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TEACHERS
TEACHER MANUAL

• Teacher manual is meant to SUPPORT the students

– Does not have lab procedures restated
• Appendix:
– Lab 1.3 – optional
– Clone that gene
– DNA ladder diagram
– Bacterial growth predictions

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LAB MANUAL - OV

• Curriculum overview
– BIG IDEA!
– Connections to academic standards
• NGSS
– Assumption of prior knowledge & skills
– Assessing student learning
– Lab Safety
– Materials and Prep

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LAB MANUAL – PROGRAM INTRODUCTION
• Overview
• Assumptions of prior knowledge
– DNA, RNA, proteins
• Learning goals
• Assessed outcome
• Suggested sequence
– Session 1, 2, etc.
– Optional lab recommendations – DNA
extraction
• Preparation
• Materials and reading activities
• Teaching
– Goes through each of the sessions

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LAB MANUAL – ALL LABS
• Labs:
– Overview
– Assumptions of prior knowledge
– Learning goals
– Assessed outcome
– Suggested sequence
• Session 1, session 2, etc.
– Preparation
– Teaching (based on suggested sessions)
– Optional
– Flowcharts
• Suggestions:
– Lab notebook
– Flow charts laminated on tables

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LAB MANUALS

• Manuals
– All sequences available online for print
• Website; curriculum; teacher/student guides
• Teacher guide password protected:
– “merlot”
– Do NOT write in the student books
– If no longer using the program PLEASE return all lab manuals

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