Comment:

This is not a real presentation !

It is a collection of slides to illustrate a possible
layout for a time slot of 15 minutes

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 1

 Comment:
What matters in an oral presentation

• Verbal (7%) – what speaker says

• Vocal (38%) – how it’s said • pitch, enthusiasm,
inflection/intonation
• Visual (55%) – speaker appearance • eye contact,
facial expressions, gestures, posture

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 2

 Comment: Things to do in an oral presentation

• Consider who your audience is.

• What do you want the audience to take from your presentation?
• Plan and practice your presentation & timing
• Use appropriate graphics
• Do not use distracting PowerPoint animations
• Show enthusiasm
• Engagement, posture, movements, perceived comfort level
• Speak to the audience – make eye contact - vocal pitch, tone, volume,
“place-holders” (like, um, actually, …)
• Consider, in advance, how you will answer questions
Henrique Leonel Gomes (hgomes@uc.pt)
Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 3

 Things to do:

• Personalize your talk (humor, quotes…)

• Speak loudly, vary your tone and pace
• Stay within your allotted time
• Make eye contact
• Ask questions - Practice!!!

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 4

 Things to do:

• Personalize your talk (humor, quotes…)

• Speak loudly, vary your tone and pace
• Stay within your allotted time
• Make eye contact
• Ask questions - Practice!!!

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 5

 Other aspects

• Professional (use corporate templates)

• Make sure you give credits to you co-workers
(Show their faces)
• Give a bit of personal touch (pictures of your
university, your Lab, etc..)

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 6

 Suggestion: List of questions and answers
(add at the end of your presentation, in case you need, as a back-up slides)

Question 1:

What is the noise level of your

system?

Answer:
Is approximately 0.3 µVpp

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 7

Sensor to measure bioelectricity from cells

Henrique Leonel Gomes

Supervisor: Prof. Sábio Silva

Sponsored by:

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 8

 Outline
• Motivation
• Objectives
• State-of-the-art /competing technologies
• Constrains
• Design of the sensor/hardware
-Basic building blocks / our design
-Detection mechanism
-Experimental set-up/prototype/demonstrator
-The electrical recording system
• Software application
• Interaction with the user
• Performance and specifications
• Economical analysis
• Conclusions
• Acknowledgments

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 9

 Objectives and motivation

Robotic dispenser
Living cells
• Our goal is to fabricate and characterize a sensor capable to
measure how biological cells react to new pharmaceutical
products.

• We developed a sensor to measure the bioelectricity of the cell Electronic

sensor
upon exposure to the new drug. (transistor)

• Our technology will operate as a screening platform for

pharmaceutical products.

• The major impact of our device is to lower the costs of clinical

trails.

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 10

 Constrains

Our sensor is designed to comply with the following specifications:

• Thermal noise level below 10 μVrms in a bandwidth of 10 kHz.

• Should provide a sensing surface capable to measure form 100 to1000 cells

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 11

 Competing technologies

Currently available technology do not meet the requirements to measure populations

of cells, because they have a high thermal noise level ( >10 µVrms.) 1

Figure 1. (A) Glass multi-/micro-electrode array (MEA) chip used to detect field potential (FP) of cells.
(B) Cells seeded on an MEA surface, grown on top of the electrodes (black dots). Figures sued from the
reference. Sophie Kussauer, Robert David and Heiko Lemcke, “hiPSCs Derived Cardiac Cells for Drug and Toxicity
Screening and Disease Modeling: What MicroElectrode-Array Analyses Can Tell Us”, published in Cells 2019, 8, 1331;
doi:10.3390/cells8111331.

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 12

 Design of the sensor

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 13

 The detection mechanism

Cell

vsignal (t)

isignal (t)

RD CD CD controls Rspread
RD is a noise
the electrical
generator iRD (t)
coupling
RD CD

Susbtrate ic(t)=CD(dv(t)/dt)
iS (t)

The interface between the cell and the

transducer is modelled by a parallel RC
circuit.

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 14

 Prototype of the sensor device

Schematic diagram showing the sensor The sensor mounted in a support capable to
connected to the low noise amplifier. hold the cells.

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 15

 The electrical recording system

Faraday cage

Low-noise Digital signal

Incubator pre-amplifiers Analyzer (DSA) PC

A CH 1
Data
RS-232
bus
Transducer GPIB USB
devices

A CH 2
RS-232
RS-232

Isolated ground
Data bus

(a)
Schematic diagram showing how the low noise amplifier and to the signals
analyser and computer are connected in the overall recording system
(1) Data acquisition (2) Data collection (3) I/O Toolkits

Henrique Leonel Gomes

CH 1 (time/spectrum):
(hgomes@uc.pt)
Experiment 1: folder1 Main menu
Pr jeto II
LEEC

- Engenharia
DEEC Departamento de Voltage Eletrotécnica e de Computadores
- CH 1: data0, 1, …, n 16
- Current
 Software to design diagrams: The Whimsical

Suggestion by João Martinho Forte da Cunha

A platform to make beatiful diagrams:
https://whimsical.com/

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 17

 Application for data analyses and processing
A program in MatlabTM was implemented. This programs controls the data
acquisition and also performs basic data analysis.

Pedro Inácio

Example of the flowchart for the software

Credit: http://www.high-voltage-lab.com/268/ibutton-electronic-lock

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 18

 User interface

The user interface provides to the

access to all parameters to run the
experimental recordings.
Furthermore the user has a range of
options to visualize the data.
The figure shows the data represented
both on the time domains as well as in
the frequency domain.

(a)

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 19

 Sensor performance evaluation

The thermal noise of our system is 0.3 μV peak-to-peak in a bandwidth of 10 kHz

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 20

Demonstration: Cancer cell migration

Ana Mestre

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 21

 Economical analysis

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 22

 Conclusions

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 23

Acknowledgements
Thank you for your
attention!
Instituto de Telecomunicações

Ana Mestre

Pr jeto II
Prof. Nikola Tesla Adriana Cavaco Joana Canudo Pedro Inácio
LEEC

Henrique Leonel Gomes (hgomes@uc.pt)

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 24
 Back-up slides

Please add, after the end of your presentation a series of back-up slides
containing additional technical details that may be of interest to the audience
during the discussion period. These slides have been prepared in anticipation of
potential questions or to provide a more in-depth explanation of certain
complex technical aspects that were not covered in the main presentation.

Feel free to refer to these slides as needed during the discussion. They are
designed to serve as a resource to address specific inquiries or to discuss
deeper into any technical subject matter that may arise.

Henrique Leonel Gomes (hgomes@uc.pt)

Pr jeto II
LEEC

DEEC Departamento de Engenharia Eletrotécnica e de Computadores 25