BHUPAL NOBLES UNIVERSITY

UDAIPUR (RAJASTHAN)

Presentation On :- "BASICS OF SPECTROSCOPY"

Of
BACHELOR OF PHARMACY
In
PRACTICE SCHOOL (CODE : BP706PS)
Supervised By :- Submitted By:-

DR. PRIYADARSHINI R. KAMBLE MAM

Dr. Siddhraj Singh Sisodiya RIYA LODHA

Associate Professor Principal ROLL NO.- 87694

Department Of Pharmaceutical Chemistry B.N. Institute of Pharm. Science Enrollment No. PHA19/7694

BNCP
DEPARTMENT OF PRACTICE SCHOOL
 Content

 Introduction of Spectroscopy
 Various others terms
 Principle of Spectroscopy
 Instrumentation of Spectroscopy
 Types of Spectroscopy
 Advantages and Disadvantages of Spectroscopy
 Application of Spectroscopy
Introduction of Spectroscopy :-
Spectroscopy
MEAN OF SPECTROSCOPY :-

derived from two simple words.

DEFINITION OF SPECTROSCOPY :-
It is the branch of science that deals with the study of interaction of electromagnetic radiation with matter.
EXAMPLE OF SPECTROSCOPY :-

Determining the atomic structure of a sample.
Spectrum Skopin
It is the band of diffrent Examination
colors formed due to or
diffrence in wavelength Evaluation
 Various others terms :-

1. EMR (Electromagnetic radiation) :- Electromagnetic radiation is a form of energy that propagates as

both electrical and magnetic waves traveling in packets of energy
called photons.

2. Wavelength:- The distance between identical points on a wave for one wave cycle.

3. Frequency:- Frequency is the number of occurrences of a repeating event per unit of time.
 Principle of Spectroscopy :-
The basic principle shared by all spectroscopic techniques is to shine a beam of electromagnetic radiation
onto a sample, and observe how it responds to such a stimulus.

The absorbed or emitted radiant energy by the sample or object. Here, the beam of electromagnetic
radiation like infrared rays, UV rays..etc are passed on the sample and the response of the sample is
measured through the wavelength of the electromagnetic spectrum applied from the external energy
source.
 Instrumentation of Spectroscopy :-

1. Radiant Energy Source (Light Source) :- Materials that can be excited to high energy states by a high
voltage electric discharge or by electrical heating serve as
excellent radiant energy sources.

2. Wavelenght Selector (Monochromator) :- Monochromator converted polychromatic light into a

monochromatic light.

a. Prisms :- Prisms convert white light into a seven rainbow colors (Spectrum).
b. Grating :- Grating alter polychromatic light into a monochromatic light.

3. Transport vessels (cuvettes) :- To hold the sample & samples to be studied in the ultraviolet or visible
region are usually glasses or solutions and are put in cells known as “CUVETTES”.

4. Detector :- Detector are generally use to convert light into a electrical signals. Which display on to the
recorder.
 Types of Spectroscopy :-
 Advantages of Spectroscopy :-

 Less expensive.
 Fast and easy.

 Disadvantages of Spectroscopy :-
 Cannot be used for metals or alloys.
 Works best for pure substances but not for complex mixtures.

 Application of Spectroscopy :-

 Determining the atomic structure of a sample.

Determination of Impurities.
 N K
H A . . .
T OU
Y