B.Sc.

Biotechnology Semester III BBT 301: Practicals in Microbiology II

1 2 3 4 5 6 7 8 9

Total

19 P
2P 2P 2P 3P 1P

Isolation of auxotrophs Observation of bacterial motility hanging drop, swarming growth methods Maintenance and revival of cultures through traditional and modern methods. Enrichment of Cyanobacteria and chemolithotrophic bacteria Microscopic examination of pathogens: amoeba (amoebiasis), Vibrio cholerae (cholera), Salmonella typhi (typhoid), Mycobacterium tuberculosis (TB) Isolation of bacteriophages Isolation of fungi and yeast from natural environment To study the growth curve of E. coli for doubling time. Plotting graph using spread sheet program. Bacterial conjugation References 1. Source Book for Experiments for the Teaching of Microbiology, Primrose, S. B. and Wardlow, A. C., Academic Press, London (1982). 2. Laboratory Exercises in Microbiology, Harley, J. P. and Prescott, L. M., McGraw Hill Publishing Co., New Jersey(2002). 3. Laboratory Manual and Workbook in Microbiology, Morello, J., Mizer, H. and Granato, P., McGraw Hill Publishing Co., New Jersey (2003). 4. Microbiology: A Laboratory Manual, 6th Edn., Cappuccino, J.G. and Sherman, N., Pearson Education Pvt. Ltd., Singapore (2004). 5. Practical Microbiology: Principles and Techniques, 1st Edn., Kale, V. and Bhusari, K., Himalaya Publishing House, New Delhi (2005). 6. Introductory Practical Microbiology, Mu dili, J., Narosa Publ. House P. Ltd., New Delhi (2007). 7. Experiments in Microbiology, Plant Pathology and Biotechnology, 4th Edn., Aneja, K. R., New Age International Publishers, New Delhi (2007).

3P 3P 2P 3P