K THUT ELISA

Enzyme-Linked
Immunosorbent Assay
GVHD: TS,H VIT TH

NI DUNG TRNH BY
I. C s l thuyt.
1.Khi nim.
2.Phn loi
II. Sandwich Elisa
1.Phng php tin hnh
2. Thit b Elisa
III. Phm vi & ng dng

I. C S L THUYT
-Thnh phn tham gia: khng nguyn,
khng th v cht to mu. Thc hin
qua hai bc:
+ Phn ng min dch hc:
+ Phn ng ha hc:

PHN LOI

2.SANDWICH
ELISA
4.COMPETITIVE
ELISA
3.INDIRECT
1. DIRECT ELISA
ELISA

SO SNH
Direct ELISA

Sandwich ELISA

K thut
c hiu
Vng kt hp
Kich thc,
khng gian

n gin
t
1 epitop
Khng nh
hng

phc tp
c
2 epitop
C nh hng

S khc bit
khng
nguyn

Khng phn bit Phn bit

PHNG PHP TIN HNH

(SANDWICH ELISA)
1.Cho khng th vo mi a Elisa
2., ra
3.Thm khng nguyn vo
4., ra
5.B sung khng th c gn enzyme
6., ra
7.Thm c cht to mu
8., c kt qu

SANDWICH ELISA TRC TIP

SANDWICH ELISA GIN TIP

THIT B ELISA
MY
O
LC
MY
ELISA
QUANG
PH
PIPETTES
KHAY NHA

C KT QU

c kt qu bng my c ELISA bc sng

450nm/ 620 nm
Nhn nh kt qu
Gi tr ca: OD chng dng/OD chng m 2
- Gi tr ca mu chng nn: OD BLank OD
chng m 0,200
- Mu xt nghim dng tnh khi: OD mu
th/OD chng m 0,200

CC YU T NH HNG.
-i lc ca khng th - khng nguyn.
- Blocking
- V tr khng gian ca epitop
- S lng khng th th nht c
gn vo y ging

PHM VI & NG DNG

Dng pht hn nhiu loi protein,
nhn din virut, vi khun,mt s hp
cht phn t lng nh trong nhiu mu
sinh hc khc nhau.
S dng trong nhiu lnh vc nh y
sinh nng nghip c bit l trong kim
tra an ton cc sn phm sinh hc.

 Xc nh nng ca khng th
trong huyt thanh
Kim tra s c mt ca khng
nguyn
Pht hin cc yu t c kh nng gy
d ng trong thc phm
Xc nh s c mt ca dc phm

 1. http://
tailieu.vn/doc/tieu-luan-cac-ky-thuat-elisa-1319697.html
2. http://
www.nitzipper.com/en/applications-bioconjugatio
n-Nitzipper/ELISA.html?gclid=Cj0KEQjw7b-gBRC45u
LY_avSrdgBEiQAD3Olx9Xc5xyP7Lv5ortMIpm-xuzhpnhtg
QzftjTTGwhhLW8aAoZm8P8HAQ
3. https://
www.idexx.com/pdf/en_us/livestock-poultry/elisa-technica
l-guide.pdf
4. http://www.case.vn/vi-VN/34/96/114/details.case

TI LIU THAM KHO

THANKS FOR
YOUR
ATTENTION !!!