Cell Disruption by Ultra-sonication - Calculations by differential

method
dC
kt
=C m k e =k [C mC ]
dt

C=Cm[1 - e-k. t];

Plot

C
t

vs. C ;

C
t

is calculated by central difference method.

t, sec
0
30
60
90
120
150
180

Eg.,

C
t

C, mg/mL
0
C30
C60
C90
C120
C150
C180

for 60 seconds is calculated as follows

C C30 C90
=
t
3090
C
t

cannot be calculated for 0 and 180 seconds;

In the plot of

C
t

vs C, slope =-k and intercept= k Cm

C
t

Hence, k=

s-1

and Cm =

mg/mL