LAB EXERCISE 7 – HISTOLOGY - PART 1

REQUIRED:
Reading required:
Laboratory manual: Human Anatomy & Physiology Laboratory Manual with Rat Dissections, 3rd
Custom Edition for St. John's University, 2017. Read below for specific Exercises & Activities.

Lab Guide - Laboratory Exercise # 7 (printout)

Personal Protective Equipment and required materials:

White Lab coat (full knee length & long sleeves)

PRE LAB ASSIGNMENT:

Read over the lab guide and familiarize yourself with the tissues and the various notes.

In today’s lab, you will be provided with:

1. A Compound microscope
2. A set of microscope slides for Histology – Part 1
Usage of a Compound Microscope and Slide Care:
Before beginning this lab check with your laboratory assistant about the use and care of the compound
microscopes – remember to focus in with the coarse adjustment knob until you see your tissue, then you
should need only a slight turn of the fine adjustment knob to focus in the higher power lenses. ALWAYS
PLACE AND REMOVE THE SLIDE WITH THE 4X LENS IN PLACE.
Note:
You should take 3 hours to view each slide several times to be able to recognize the tissue without the benefit
of the label for the quiz as well as the exam.

CLASSIFICATION OF TISSUES:
Read Exercise 6, pages 67-92
Use the photomicrograph of the tissue
A) Know the distinguishing features: 1) number of layers (cell membrane) (2) Shape (3) presence or absence
of basal membrane (4) nucleus location (apical, center or basement side) 5) other features: striations, fibers.
B) Look for these features in your slide
C) Identify the tissue based on the presence or absence of the distinguishing features and relate to function.

DO NOT memorize the pictures! Each slide may show more than one tissue type in your field view. The
tissue may appear different when viewing from the top (surface) or when viewing a cross section and
magnification.

1
 General Notes on Today’s Tissues
Types of Tissues Simple Squamous Stratified Squamous Muscle Nervous

Shape

Placement of
Nucleus

Layers and
Patterns

Extra Features

I – EPITHELIUM (pg. 68-74)

Epithelial tissue covers exposed surfaces and lines the internal passageways and forms glands. Cells have
apical (free) surface and basal surface. The cells fit together to form membranes (sheets) and are supported
by connective tissue. Cells are avascular and have a basal membrane.
Classification is based on 1) number or arrangement of layers and 2) cell shape.
Pseudostratified epithelia appear multilayered but all the cells are attached to the same basement
membrane.

Simple epithelium: consists of a single layer of cells attached to a basement membrane.

Type Slide # List & Draw the Distinguishing Features

#2 – Lung and
Bronchioles
Simple
Squamous
epithelium
(Fig. 6.3a. pg.
70)
#4 – Kidney

2
Type Slide # List & Draw the Distinguishing Features

#6 – Thyroid

Simple
Cuboidal
epithelium
(Fig. 6.3b. pg. 70)

#4 – Kidney

Goblet Cells
#3 – Small
Intestine &
Stomach
Simple
Columnar
epithelium
(Fig. 6.3c. pg. 71)

#7 – Colon

#2 – Lung and
Pseudostratified Bronchioles
Columnar
epithelium
(Fig. 6.3d. pg. 71)

Cilia
#9 – Trachea

3
Stratified epithelium: consist of several layers of cells attached to a basement membrane.

Type Slide # List & Draw the Distinguishing Features

#5 – Esophagus
and Stomach
Stratified
Squamous
epithelium
(Fig. 6.3e. pg. 72)
keratinized layer
#1 – Skin

Stratified
No slide for observation (see book images
Cuboidal Fig. 6.3f. pg. 72
only)
epithelium
Stratified No slide for observation (see book images
Columnar Fig. 6.3g. pg. 73 only) (found in male urethra, large ducts of
epithelium glands)
Transitional found in bladder and linings of urinary
epithelium #8 - organs
(Fig. 6.3h. pg. 73)

4
 II- NERVOUS TISSUE (pg. 82-83)

Carries electrical signals from one part of the body to another.

Type
Figure/Page Slide # List & Draw the Distinguishing Features

Nervous #11 Spinal

Cord and
Tissue Ganglion
(Fig. 6.6. pg. 82)

III- MUSCLE TISSUE (pg. 83-85)

Specialized for contraction, skeletal muscle, heart muscle, and walls of hollow organs.

TYPES
Slide # 10 Skeletal Muscle Smooth Muscle Cardiac Muscle
Muscle Composite
Slide (Three types)

Type Slide # List & Draw the Distinguishing Features

#12
Skeletal
Muscle
(Fig. 6.7a. pg.
84)

5
 #13
Smooth
Muscle
(Fig. 6.7c. pg.
85)

Intercalated discs
#14
Cardiac
Muscle
(Fig. 6.7b. pg.
84)