BACTERIOLOGY Genera that can be visualized by Bright-Field microscope

 Borrelia
Disease caused by Staphylococcus epidermidis
 Infection associated with medical implants Specie of Borrelia that DO NOT cause relapsing fever
 Borrelia burgdoferi
Disease caused by Staphylococcus saprophyticus
 Menstruating associated with Toxic Shock Syndrome Culture media used to isolate Borrelia spp.
 Kelly
Disease caused by Streptococcus pyogenes
 Scarlet fever In the acronym R.A.C.E., A stands for…
 Alarm
Presumptive test for Streptococcus pneumonia
 Optochin susceptibility test Maximum number of antimicrobial disc in a 150mm plate
 12
Presumptive test for Staphylococcus saprophyticus
 Novobiocin susceptibility test Implies that an infection due to an organism can be appropriately treated with the recommended dose of
antimicrobial agent for that type of infection and infecting organism unless, otherwise contraindicated.
Presumptive test for Streptococcus agalactiae  Susceptible
 CAMP test
Temperature needed for transport of specimen containing viruses without significant loss of infectivity
Cultural characteristic of Staphylococcus aureus  -70°C
 Golden yellow haloin MSA
Causative agent of invasive disease of newborn
Cultural characteristic of Proteus mirabilis  Streptococcus agalactiae
 Swarms on SBA
Classification of media that DOES NOT classify BAP
Cultural characteristic of Salmonella spp., in Salmonella Shigella Agar (SSA)  Enrichment
 Non-lactose fermenter with H2S
Organisms that cause food poisoning
Cultural characteristic of Streptococcus pneumonia  Staphylococcus aureus
 alpha hemolysis on SBA  Escherichia coli
 Bacillus cereus
Cultural characteristic of Streptococcus pyogenes
 beta hemolysis on SBA High protein containing media that CANNOT withstand the temperature of autoclave
 Lowenstein-Jensen
Optimum temperature of human pathogen  Dorset’s Egg Medium
 35°C

Reproduce by transverse fission

 Treponema Spirochetes:
 Borrelia
 Leptospira
  Treponema A carrier state of Salmonella infection harbors the organism in what organ?
 Gall bladder
Gram Positive Bacteria
 Lactobacillus The EHEC strain serotype causes hemolytic uremic syndrome. What serotype was the first to have been widely
 Corynebacteria reported?
 Bacillus  0157:H7
 Nocardia
An organism that causes erythem migrans
Media used for viral cultures  Borrelia burgdoferi
 Chick embryo
 Primary monkey kidneys Pore size of membrane filter that are capable of retaining viruses in sterilizing liquids
 0.01µm
The togavirus known to produce fetal defects
 Rubella What specimen requires immediate processing when received in the microbiology laboratory?
 CSF
The “core window” refers to the time…
 during hepatitis B virus infection when anti-HBc IgM is the only serologic marker Material Safety Data Sheets are important to employees because they contain information relating to…
 Chemical Safety
A susceptibility test differentiates CoNS (coagulase negative susceptibility Staphylococci) from Micrococci spp.
 Bacitracin Possible organism if you see encapsulated yeasts cell in India ink preparation of CSF
 Cryptococcus neoformans
An organism negative for PYR test
 Staphylococcus aureus A slimy colony in blood agar medium indicates…
 capsulated organism
Culture media used as primary screening for Escherichia coli 0157:H7
 Sorbitol MacConkey Agar An organism without a cell wall
 Mycoplasma
Biochemical test that differentiates Proteus mirabilis from Proteus vulgaris
 indole and ornithine decarboxylase The result of sexual reproduction in fungi
 Ascospores
Bacteria that produces the red pigment prodigiosin
 Serratia marcescens Mumps virus is under the family…
 Paramyxoviridae
The species of Clostridium when isolated from blood indicates malignancy in the GI tract
 Clostridium septicum Kaposi’s sarcoma is caused by what virus?
 Human Hepres Virus 8
The Neufeld Quellung test detects the presence of what structure of bacteria
 capsule A virus that is predominantly associated with respiratory disease and epidemics of keratoconjunctivitis
 Adenovirus
An organism that has the characteristic of “burnt gun powder”odor
 Proteus mirabilis The type of cell culture that best supports the growth of Cytomegalovirus
 Human fetal diploid cells
Molluscum contagiosum virus is a viral member of what DNA virus? The gene code of the M protein in Streptococcus pyogenes
 Poxviruses  mecA

Influenza A virus undergoes recombinant events that produce new strains; this is referred to as… Hemolysin that Staphylococcus aureus should possess for CAMP test
 Antigenic shift  beta (β)

Orchitis, which may cause sterility, is a possible manifestation of… Disease caused by staphylococcal exfoliative toxin
 Mumps  Ritter disease

Viral agents shows the characteristic of infection with the appearance of Koplik spots in the oral mucosa of Causative agent of plague
patients  Yersinia pestis
 Measles
Chlamydospores that grows at the side of the hyphae
A bacteriophage is…  Sessile
 A virus that attacks bacteria
The following are systemic mycoses caused by “dimorphic fungi”
Negri bodies may be found in brain tissues f humans or animals infected with…  Darling’s disease
 Rabies  Paracoccidiodomycoses
 Gilchrist’s disease
The significant colony count of a random midstream catch urine
 105 CFU/ml South American blastomycosis is caused by?
 Paracoccidiodes brazilienses
What would be the next step if a gram stained smear from a 24hour culture shows gram positive cocci?
 Catalase test A general term used to describe any infection caused by a dermatiaceous organism
 Chromoblastomycosis
Acid fast stained smear shows positive for acid fast bacilli. There were 59 acid fast bacilli counted in 100 fields.
How will be the grade of your count?
 1+
Significant cause of human cervical cancer
 Human Papilloma Virus
What would be the grade of a (0-4/OIF) pus cell count in direct sputum specimen?
 Occasional A member of family Herperviridae that causes Infectious Mononucleosis
 Epstein Barr Virus
What is the medium used for colony count in URICULT?
 CLED agar Mumps virus is under this family
 Paramyxoviridae
The colonies in BAP were tested positive for germ tube test. What is the pathogen present in the culture?
 Candida albicans Human immunodeficiency virus types 1 & 2 is under the family…
 Retroviridae
What would be the color of acid fast bacilli if you omit decolorization in the staining procedure?
 Red Kaposi’s sarcoma is caused by this virus
  HHV8 (human herpes virus 8)  Staphylococcus
 Stomatococcus
How long should the plates dry before adding the disks after inoculating the Mueller-Hinton agar plates for the  Planococcus
Kirby Bauer susceptibility test?  Streptococcus
 5-10 minutes
Staining method used for detection of Mycobacterium tuberculosis
Mordant used in Zeihl-Neelsen acid fast stain is?  Zeihl-Neelsen
 Heat  Truant
 Kinyoun
Preferred medium for the culture of Haemophilus species
 Enriched chocolate agar Carbohydrates used in TSI
 Sucrose
Organism that gives a “stormy” fermentation in milk media due to excessive gas production  Lactose
 Clostridium perfringens  Glucose

Positive oxidase test gives this color Filters used for sterilization
 Purple  paper mask
 dry gauze
Morphological forms of Chlamydia  cotton plug
 Reticulate body  HEP-A filter
 Elementary body
Death of microorganism by ultraviolet light is the result of absorption of radiation by…
Cutaneous staphylococcal infections  DNA
 impetigo
 folliculitis
 carbuncle
 boil Listeria monocytogenes should be considered if a beta hemolytic colony on defibrinated sheep’s blood agar
demonstrates…
Somatic antigens of Proteus vulgaris that is used in Weil-Felix reaction  transparent colonies on any agar
 OX-2
 OX-K “Satellite phenomenon” is illustrated when Haemophilus influenza colonies grows luxuriantly next to…
 OX-19  Staphylococcus aureus

Hydrogen sulfide positive Rod-shaped organisms that formed endospores belong to the agent…
 Arizona  Clostridium
 Edwardsiella
Gram stained smears from fecal specimen are…
Mode of actions of antimicrobial chemotherapeutic agents  best for isolation
 inhibit protein synthesis
 interferes with DNA synthesis Demonstration of bacterial motility is done in?
 semi-solid medium
Member of Micrococcaceae
Components of the McFarland nephelometer standard
 BaCl3 & H2SO4 Organisms which primarilylive in soil and are spread to human contact with soil
 geophilic
Inhibits growth of gram positive bacteria
 bile salt The purpose of the standing for 10-30 minutes before microscopic examination when preparing for KOH
preparation for fungal elements
To demonstrate beta hemolysis of some species of Haemophilus hemolyticus and Haemophilus parahemolyticus  clearing
which blood sample is recommended?
 sheep’s blood Lactic acid in Lactophenol Cotton Blue Stain…
 preserves fungal structures
H2Sindicators
 Ferrous sulfate In Papanicolau Stain, fungal elements stain…
 ferric ammonium citrate  blue
 ferric citrate
 sodium citrate Calcofluor white staining requires use of…
 fluorescent microscope
Ethylene oxide
 Bacillus subtillis var globjii Germ tube test is specifically for detection of…
 Candida albicans
Hot air oven
 Bacillus subtilis var niger Spotted fever group
 South African tick fever
Autoclave  Rickettsial pox
 Bacillus stearothermophilus  American spotted fever

Ionizing radiation pH indicators

 Bacillus pumilis  methyl red
 bromthymolblue
Characteristic of fungi
 Lacks chlorophyll Toxic products of oxygen
 hydrogen peroxide
The result of sexual reproduction of fungi  superoxide dsmutas
 Ascospores
Tissue culture media
Causative agent of black piedra  HeLa cells
 Piedraia hortae  Human embryonic kidney
 primary monkey kidney
Causative agent of white piedra  footpad of mice
 Trichosporon bieglii
Require living cells for growth
Causative agent of ptyriasis verscolor  Chlamydia
 Malassezia furufr  Rickettsiae
  Viruses Borrelia burgdorferi
 erythma chronicum migrans
Enterics that are not considered as part of the normal intestinal flora
 Salmonella Legionella pneumophilia
 Shigella  Pontiac fever

Specimen collection for anaerobic culture

 percutanous aspration of sputum
 thoracentesis for sputum
 suprapubic aspiration of urine

Fluorescent dyes and tags

 acridine orange
 calcofluor white

Anaerobic organisms
 Bacteriodes
 Veilonella
 Clostridium
 Actinomycetes

Capnophilic organisms
 Eikenella
 Haemophilus
 Cardiobacterium

Antimicrobial agents that inhibits peptidoglycan synthesis

 rifampicin
 aminoglycosides

Spotted fever group of Rickettsial infections

 rocky mountain spotted fever
 Japanese spotted fever

Disease produced:
Haemophilus influenza biotype III
 “pink eye”