Experiment No.

Water Analysis: Dissolved Oxygen

Name: De Juan, Jarell P.

Couse and Section: CM011L-E01

INTRODUCTION
The aim of this experiment was to investigate the level of dissolved
oxygen (DO) using a variety of techniques and equipment in a sample of water from an
unknown source and to establish the biochemical oxygen demand (BOD) in a number of
different water sources. The level of DO in water is dependent on many physical, chemical, and
biochemical factors. For instance, aeration, algae, temperature, wind, velocity of water flow,
atmospheric pressure, salt content, organic compounds, bacteria and animals. The temperature
will determine the saturation point oxygen in water. The colder the water, it will hold more
oxygen. Increasing the temperature will normally decrease the DO concentration in water. It
is important to distinguish between oxygen solubility, which is the maximum DO
concentration at equilibrium, and the actual concentration of DO, which is generally not the
equilibrium concentration and is limited by the rate at which oxygen dissolves.

The objectives of the Experiment are:

 To measure the amount of dissolved oxygen in a sample of water using the Winkler
method.

EXPERIMENTAL SECTION

Chemicals

The chemicals that are used in the experiment are water, manganese salt, iodide ions, and
sodium thiosulfate.

RESULTS AND DISCUSSION

−¿+ O2 (aq) →2 MnO 2( s)+ 2 H 2 O(I ) ¿
2+¿+4 OH (aq) ¿
Step 1: 2 Mn(aq )
−¿ +¿ 2 +¿ 2
Step 2: Mn O 2(s )+ 2 I (aq )+ 4 H (aq) → Mn(aq) + I 2(aq) +2 H O (I) ¿ ¿ ¿ −¿
2−¿+ 2 I(aq ) ¿¿

Step 3: 2 S 2 O 3(aq)2−¿+ I 2(aq) → S 4 O6(aq) ¿

Ratio:
2 S 2 O3(aq)2−¿¿: I 2(aq) 2:1

Mn O 2(s ): I 2(aq) 1:1

O 2(aq) :2 Mn O 2(s) 1:2

Ratio of O2 to S2 O 32−¿ ¿ is 1:4, which means that 1 mole of oxygen reacts to 4 moles of S2 O 32−¿ ¿.

Calculations:
12.50
n (S2 O32−¿ ¿) = 0.0500 × =6.25 ×10−4 mol
1000

We know that O2 : S 2 O 32−¿¿ 1:4

Finding the no. of moles of Oxygen: n (O2) ¿ 6.25 ×10−4 : 4=1.56 × 10−4 mol

n 1.56 ×10−4
C= = ¿ 3.12× 10− 4 mol dm−3
V 500 ÷ 1000

Calculate the concentration of dissolved oxygen in g/dm:

m ( O2 )=1.56 ×10−4 ×32.00 ¿ 4.99 ×10−3 g ¿ 4.99 ×10−3 × 2 ¿ 9.98 ×10−3 g dm−3

9.98 ×10−3 g dm−3 =9.98 ppm

The resulting dissolved oxygen is 9.98 ppm in the 500 cm3 sample of water. Furthermore,
in determining the dissolved oxygen in the water sample, we first calculated the amount of mole
of thiosulfate ions, which gives us the resulting 6.25 ×10−4 mol. Since, the ratio of oxygen to
thiosulfate is 1:4, we were able to determine the number of moles of oxygen, that is,
1.56 ×10−4 mol. Afterwards, we can calculate the concentration of dissolved oxygen, using the

n
equation C= , this gives us 3.12 ×10−4 mol/dm−3. Lastly, using the calculated values, solving
V
the concentrated oxygen grams per dm-3, which results in 9.98 ×10−3 g ¿ dm−3 or 9.98 ppm,
meaning that the dissolved oxygen in the 500 cm3 water is 9.98 ppm.

CONCLUSION
While titration is an accurate method of establishing DO, it is really a laboratory tool and will
take up valuable time before a result is made, any time wasted could result in major fish kills due
to lack of dissolved oxygen in the water system. The use of DO probes are a necessity for any
aquaculturist in maintaining water quality, once calibrated they provide a quick and reliable
results that can be acted upon.

ACKNOWLEDGEMENTS
This experiment was made possible by the following (YouTube channels):
 Mike Sugiyama Jones
 Pdst Chemistry
 And to Mrs. Elizabeth Espiritu, for helping us to understand the concept of this
experiment better and its applications in the real world.

REFERENCES
Fondriest Environmental, Inc. (2013, November 19). Dissolved Oxygen. Retrieved from
Fundamentals of Environmental Measurements:
https://www.fondriest.com/environmental-measurements/parameters/water-
quality/dissolved-oxygen
Myers, S. (2018, May 22). AERATION BASICS: WHY DOES THE DISSOLVED OXYGEN
CONTENT OF WASTEWATER MATTER? Retrieved from JMS.Inc.:
https://www.jmsequipment.com/about/