Groups of Name Components Purpose

mediums
Liquid 1. broth Hottinger Extract of meat multiplication
2. serum broth multiplication
Solid 3. agar in Petri dishes Agar-agar Isolation the colony
4. the bent agar Agar-agar Accumulation
the microorganisms
A. Elective 1. blood-agar 5-10 % coccus
2. biliated broth 10 % salmonella
3. coagulated serum 5-10 % C. diphtheriae
4. sugar broth 1% Streptococcus
B. Enrichment 1. Muller Salmonella
2. Kauffmann Salmonella
3. Kitt Tarozzi Liver Clostridium
4. with selenite Shigella
C. Differentially 1. Hiss Diverse sucrose enterobacteriaceae
-diagnostical 2. Endo Sol. fuxine E.coli
3. Levine enterobacteriaceae
4 Ploskirev Shigella
5. Russel enterobacteriaceae
6. Kligler enterobacteriaceae
D. Special 1.Klauberg, Tinsdal C. diphtheriae
2.Finn, Popescu M.tuberculosis
3.Sabouraud Candides
E. Transport 1.sol. NaCl Enterobacteries
2.sol.tampon-phosphate Enterobacteries
3.glicerine mix 0,85 % NaCl + Enterobacteries
30 % glicerine

1. Study colonies on different mediums and fill enter the result in the table:

Assessment criteria Studied colonies

Nr 1 tip R Nr 2 tip S Nr 3 tip Mix
1. Size 3-5 mm 1-3 mm 2-3 mm
2. Transparency Non transparent Transparent
transparent
2.Pigmentogenesis present absent present
4. Surface Rough (raff) smooth smooth
5. Color Yellow-grey wait Yellow
6. Edges Irregular regular regular
7. Structure nehomogeny homogeny homogeny
8. Consistency dry mucous mucous
9. Type of colony R S M

Bacteria enzymes. Classification.

Constitutive enzymes –
lipase,
carbohydrate,
proteinase,
oxidase

Inductive enzymes – de adaptare, de agresie:

penicilinase,
decarboxilase,
fosfatase,
β-galactosidase,
hemolisine,
fibrinolisine,
collagenase,
lecitinase…