ACADEMIC YEAR 2019 - SEM2 - GENETICS ASSIGNMENT 1- MAY

FULL NAME: 1. STUDENT ID: 1.

2. 2.
3. 3.
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QUESTION 1 (40 marks): Check the expression of indicated enzymes (USING “+” for expression, “-“ for no expression) in E.coli strains with the following
genotypes

β-galactosidase Permease
Genotype
Lactose present Lactose absent Lactose present Lactose absent

lacI+ lacP+ lacOc lacZ - lacY+/lacI – lacP - lacO+ lacZ+ lacY+ _ _ + +

lacIs lacP - lacO+ lacZ+ lacY -/lacI - lacP+ lacO+ lacZ – lacY+ _ _ + +

lacI - lacP+ lacOc lacZ+ lacY+/lacI+ lacP - lacOc lacZ+ lacY+ _ _ _ _

lacIs lacP - lacOc lacZ+ lacY+/lacI- lacP+ lacO+ lacZ+ lacY - + + _ _

QUESTION 2 (60 marks): Provide 2 possible diploid genotypes that can produce the following products in EACH indicated condition.

Lactose present Lactose absent

β
β
Genotypes
-galactosidas Permease Permease
-galactosidase
e

G1: lacI+ lacP+ lacOc lacZ - lacY+/ lacIs lacP - lacO+ lacZ+ lacY -
- + - +
G2: lacIs lacP - lacO+ lacZ+ lacY -/ lacI - lacP+ lacO+ lacZ – lacY+

G1: lacI+ lacP+ lacOc lacZ - lacY+/lacI- lacP+ lacO+ lacZ+ lacY -
+ - + +
G2: lacI – lacP - lacO+ lacZ+ lacY+/lacI - lacP+ lacO+ lacZ – lacY+

G1: lacI - lacP+ lacO+ lacZ – lacY+/lacI- lacP+ lacO+ lacZ+ lacY -
+ + - +
G2: lacI - lacP+ lacO+ lacZ – lacY+/ lacI+ lacP+ lacOc lacZ - lacY+