NANYANG JUNIOR COLLEGE

JC 1 BLOCK TEST
Higher 2

CANDIDATE
NAME

CLASS

BIOLOGY 9744/01
Paper 1 Multiple Choice 26 June 2020
30 minutes
Additional Materials: Multiple Choice Answer Sheet

READ THESE INSTRUCTIONS FIRST

Write in soft pencil.

Do not use staples, paper clips, highlighters, glue or correction fluid.
Write your name and CT on the Answer Sheet in the spaces provided unless this has been done for you.

There are fifteen questions on this paper. Answer all questions. For each question there are four possible
answers A, B, C and D.
Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet.

Read the instructions on the Answer Sheet very carefully.

Each correct answer will score one mark. A mark will not be deducted for a wrong answer.
Any rough working should be done in this booklet.
Calculators may be used.

This document consists of 9 printed pages.

[Turn over

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1 Disaccharides are formed following synthesis from monosaccharides or as a result of
polysaccharide hydrolysis.

Cellobiose, maltose, sucrose and trehalose are four different disaccharides found in nature.

Some features of these disaccharides are listed.

• The disaccharide cellobiose is formed from the hydrolysis of the polysaccharide
cellulose.
• Sucrose is composed of glucose and fructose.
• Trehalose is a non-reducing disaccharide that is synthesised from two α-glucose
molecules.
• The disaccharide maltose is formed from the hydrolysis of amylose, a component of
starch.

Which column correctly identifies each disaccharide?

A B C D

cellobiose maltose sucrose trehalose

maltose cellobiose trehalose maltose

sucrose trehalose cellobiose cellobiose

trehalose sucrose maltose sucrose

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2 Which statement is true for phospholipid, but not for protein?
A Its molecules have hydrophilic and hydrophobic components.
B Its molecules are synthesised from non-identical sub-units.
C It is a barrier to water soluble molecules.
D It is found in cell membranes.

3 The diagram shows four different amino acids, each with a different R group (side chain).

Which amino acids could form a hydrophobic interaction between their R groups?
A 1 and 2
B 1 and 3
C 2 and 4
D 3 and 4

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4 Which features affect the tensile strength of collagen?
1 the helical structure of collagen chains
2 the small R group of the amino acids in collagen
3 the insoluble nature of collagen
4 the covalent bonds between collagen molecules

A 1, 2, 3 and 4
B 1 and 3 only
C 1, 2 and 4 only
D 2, 3 and 4 only

5 Four students were asked to match the function with the appearance of some cell structures
in an animal cell.

The function were listed by a number:

1 organises microtubules to produce the spindle during cell division

2 synthesis of polypeptides
3 synthesis of lipids

The appearances were listed by a letter:

V membranes which surround an enclosed inner cavity

W non-membrane bound, spherical structures
X a double membrane interspersed with pores
Y non-membrane bound, cylindrical structures
Z membrane-bound sacs, arranged as a flattened stack

Which student correctly matched the numbered functions with the appearance of the cell
structure?

1 2 3
A W X Z
B W Z V
C Y W V
D Y Z W

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6 A scientist carried out an experiment to separate cell structures in animal cells.

The cells were broken open to release the cell structures.

This extract was filtered into a centrifuge tube and then spun in a centrifuge. The heaviest cell
structure sank to the bottom forming pellet 1, as shown in the diagram.

The liquid above pellet 1 was poured into a clean centrifuge tube and spun in the centrifuge
at a higher speed to separate the next heaviest cell structure. This cell structure sank to the
bottom, forming pellet 2.

This procedure was repeated twice more to obtain pellet 3 and pellet 4, each containing a
single type of cell structure.

Which row shows the order in which the cell structures were collected?

pellet 1 pellet 2 pellet 3 pellet 4

A nucleus lysosomes mitochondria ribosomes
B nucleus mitochondria lysosomes ribosomes
C ribosomes lysosomes mitochondria nucleus
D ribosomes mitochondria lysosomes nucleus

7 The diagram represents part of the fluid mosaic model of a cell surface membrane.

Which parts of the membrane are hydrophilic?

A 1, 2 and 3 B 2 and 3 only C 3 and 4 D 4 only

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8 When investigating the rate of reaction of the enzyme lipase on the hydrolysis of triglycerides,
the pH must be maintained at an optimum to prevent the lipase denaturing.
What is the reason for this?
A The addition of water molecules produced by hydrolysis increases pH.
B The addition of water molecules produced by hydrolysis decreases pH.
C The products of hydrolysis decrease the pH.
D The products of hydrolysis increase the pH.

9 A fixed volume of the enzyme catalase was added to a fixed volume of hydrogen peroxide
solution. The diagram shows how the rate of the reaction changed over the course of the
reaction.

Why did the rate of reaction decrease over time?

A The enzyme active sites become saturated.
B The enzymes were denatured.
C The product inhibited the reaction.
D The substrate molecules were used up.

10 Which statement about tRNA is not correct?

A Hydrogen bonds between bases temporarily hold tRNA against mRNA.
B The base sequences in the tRNA molecules are the same as the base sequences in the
DNA that is being translated.
C tRNA translates the base sequence in DNA into the amino acid sequence in a protein.
D The specificity of a tRNA molecule depends on the specificity of the enzyme that loads it
with an amino acid.

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11 The diagram shows part of a nucleic acid.

Which row correctly describes the bonds shown in the diagram at positions 1, 2, 3 and 4?

is easily broken by
can be found in is formed by is formed during
changes in
RNA condensation DNA replication
temperature or pH

A 1, 2 2, 3 1, 2, 3 4

B 3, 4 1 2, 3 2, 3

C 1, 2, 3 1, 2 1, 4 3, 4

D 1 1, 3, 4 1, 2 3, 4

12 A peptide consists of ten amino acids of four different kinds.

What is the theoretical minimum number of tRNA molecules required to translate the mRNA
for this peptide?

A 4
B 10
C 12
D 30

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13 The diagram shows the classification of several portions of eukaryotic DNA that do not
encode RNA or protein.

Which combination correctly identifies the type of eukaryotic DNA?

V W X Y Z

A centromere intron telomere enhancer 5’ UTR

B centromere enhancer telomere intron 5’ UTR

C intron enhancer telomere 5’ UTR centromere

D telomere enhancer intron 5’ UTR centromere

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14 Recombinant DNA techniques can be used to alter the locations of control elements within
DNA sequences. These techniques allows scientists to study the effects of such location
changes of control elements on the levels of transcription.
The diagram shows the various structures of transcription units and the corresponding
relative levels of transcription.
The promoter, enhancer and coding sequences are represented by letters P, E and G
respectively. The number of symbol ‘+’ indicates the relative frequency of transcription.

With reference to the diagram, which of these statements is a valid conclusion?

A The relative distance between promoter and enhancer has no effect on the frequency
of transcription.
B The frequency of transcription is increased when the enhancer is located upstream of
the promoter.
C An enhancer is required for transcription.
D Orientation of the enhancer does not affect the frequency of transcription.

15 Each cycle of the polymerase chain reaction (PCR) has three steps.
• DNA denatures to produce separate strands.
• Single-stranded DNA anneals with primers.
• Taq polymerase elongates the primer strands.
Which statements about Taq polymerase are correct?
1 Taq polymerase is not denatured by the temperature of the DNA denaturation
step.
2 Taq polymerase is needed for binding primers to the separated DNA strands.
3 Taq polymerase has a high optimum temperature therefore the temperature can
be raised after annealing for efficient elongation.
4 Taq polymerase does not have to be replaced during each cycle.

A 1 only B 1 and 2 only C 3 and 4 only D 1, 3 and 4 only

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