Z3 Examination Name: ________________

Completion (2’ for each blank) Score: _________________

1. Power requirement of Z3: AC 100 ~ 240 V

1. Z3 test speed: 70 tests/hour.

2. Z3 only detector the 3 main parts of blood cell. Including: RBC, WBC and PLT .

3. Z3 can provide 21 report parameters and 3 histograms (specific quantity).

4. Z3 support sample model: Whole blood model and pre-diluted model.

5. Software login password is admin.

6. Reagent Shelf life and open-vial stability date:

Reagent Diluent Lyse Probe Cleanser

Shelf life 24 months 24 months 12 months

Open-vial stability 60 days 60 days 60 days

7. When adjusting HGB gain, HGB blank value should be around 4.5±0.1V.

8. In the RBC chamber, Z3 can detector RBC and PLT. In the WBC chamber, Z3 can detector

WBC and HGB.

True or False (2’ for each blank)

2. Z3 is open system. ( × )

3. Z3 can connect LIS and external printer. ( √ )

4. There is no need to do QC or calibration when the machine is installed first time. ( × )

5. We need to use probe cleanser while turning off machine. ( √ )

6. Diluent can dissolve RBC, then release and react with HGB. ( × )

7. Lyse can be used in some maintenance procedures like unclog, and cleaning. ( × )

Question and answer

1. Where can we find the diluent barcode and lyse barcode? (5')

1. The barcode of lyse is located on the label of lyse bottle.

2. The barcode of diluent is pasted on the cap of diluent bottle.

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Floor 1 to Floor 4, Building 30, No.6 of Taikang Road,

Block C of Jianqiao Industrial Park, Dadukou District, Chongqing, China 400082
Tel: +86 (0)23 6865 5509 Fax: +86 (0)23 6869 9779 Email: info@zybio.com Website: www.zybio.com
2. What should we do if machine shows 'Reagent run out' error? (5')

Replace new reagent and load barcode as soon as possible.

3. What's the function of diluent? (10’)

1. Dilute blood cells and make an even distribution.

2. Act as a good conductor for the electric circuit.

3. Used in some maintenance procedures like unclog, and cleaning.

4. Provide an isotonic environment for blood cell.

4. What should we do if machine show 'Reagent expired' error? (10’)

1. Turn on the ‘elimination’ function. (Software operation: ‘Management’ – ‘Setup’ –

‘Reagent’ – Choose ‘One key elimination’ to turn on the function.)

2. Click the ‘Reagent expired’ error pop up then use ‘clear’ button to remove the error.

3. Test some normal samples to check the machine performance.

5. When machine provides error ‘Vacuum Pressure Abnormal’, what parts should we check?

(10’)

1. Use ‘clear’ button to remove the error.

2. Check whether the pressure is stable and within requirement range [-33, -25].

3. Check related tube connections. (Vacuum tank and liquid pump).

4. Check Vacuum tank, clean liquid pump and test pressure sensor by syringe manually.

6. If WBC target value is 4.5 x10^9/L, but the test result is 4.0 x10^9/L. Machine software

didn’t provide any error. Plus, the repeatability of the machine is very good. From the

aspect of maintenance and calibration, what we should do? (10’)

1. We need maintain the machine: aperture unclog, clean WBC/RBC chamber and probe

cleanser soak.

2. Do calibration to increase the WBC factor. (Manual calibration, calibrator calibration and

fresh blood calibration).

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Floor 1 to Floor 4, Building 30, No.6 of Taikang Road,

Block C of Jianqiao Industrial Park, Dadukou District, Chongqing, China 400082
Tel: +86 (0)23 6865 5509 Fax: +86 (0)23 6869 9779 Email: info@zybio.com Website: www.zybio.com
*This is an optional question. (10’)

Try to explain ‘Electrical impedance method’ according to this photo.

In the middle of the chamber, there is 1 aperture. Beside the aperture, there are 2 electrodes. In

the front bath, diluent mix with sample. Diluent can act as a good conductor to connect the

electrical circuit and the current of the electrical circuit is consistent.

Blood cells are poor conductors for electrical circuit. While blood cell volume is bigger, the

resistance is bigger.

Blood cells will pass though the aperture by the negative pressure from back bath. As the formula

U=R*I, the larger cell volume will make bigger voltage. Finally, the bigger impulse single will be

generated.

What’s more, how many cells go through the aperture, how many signals will be detected by

machine. So, the machine can count the cells.

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Floor 1 to Floor 4, Building 30, No.6 of Taikang Road,

Block C of Jianqiao Industrial Park, Dadukou District, Chongqing, China 400082
Tel: +86 (0)23 6865 5509 Fax: +86 (0)23 6869 9779 Email: info@zybio.com Website: www.zybio.com