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  • Estimation of Blood Urea

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    Sri Satwik Nimmagadda
    52 views5 pages
    This document describes a method to estimate the concentration of urea in a given sample using the diacetyl monoxime (DAM) method. The method involves adding DAM reagent and acid reagent to test tubes containing the sample, standard, and blank. A pink colored complex forms in proportion to the urea concentration, and the absorbance is measured at 540 nm. The concentration of urea in the sample is then calculated by comparing to the standard. Normal blood urea levels are 15-40 mg/dl. Elevated levels may indicate dehydration, kidney disease, or other renal issues.

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    This document describes a method to estimate the concentration of urea in a given sample using the diacetyl monoxime (DAM) method. The method involves adding DAM reagent and acid reagent to test tubes containing the sample, standard, and blank. A pink colored complex forms in proportion to the urea concentration, and the absorbance is measured at 540 nm. The concentration of urea in the sample is then calculated by comparing to the standard. Normal blood urea levels are 15-40 mg/dl. Elevated levels may indicate dehydration, kidney disease, or other renal issues.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    52 views5 pages

    Estimation of Blood Urea

    Uploaded by

    Sri Satwik Nimmagadda
    This document describes a method to estimate the concentration of urea in a given sample using the diacetyl monoxime (DAM) method. The method involves adding DAM reagent and acid reagent to test tubes containing the sample, standard, and blank. A pink colored complex forms in proportion to the urea concentration, and the absorbance is measured at 540 nm. The concentration of urea in the sample is then calculated by comparing to the standard. Normal blood urea levels are 15-40 mg/dl. Elevated levels may indicate dehydration, kidney disease, or other renal issues.

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    © All Rights Reserved
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    of 5

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