Meghmani Industries Limited

Quality Control Laboratory, Unit-2

Plot No. 27, Phase–1, GIDC Industrial Estate, Vatva, Ahmedabad,
Pin–382 445, Gujarat, India Tel. No.  +91–81550 02071, Fax No.  +91–79–2589 3495
E Mail  qc@mil2.meghmanidyes.com, Web  www.meghmanidyes.com

01-09-
Doc. No. W/OPN/70 ANALYSIS METHOD FOR Date of Issue
2016
Issue No. 01 METOLACHLOR TECHNICAL Page No. 1 of 2

A-1 Purpose
Metolachlor content determined by gas chromatography (GC) method.
.
A-2 Scope
These work instructions apply for determination of Metolachlor content in the Metolachlor
Technical.

A-3 Responsibility
Technical Manager /QC-officer / QC-Chemist.

A-4 Principle
Metolachlor is dissolved in Acetone along with internal standard and determined by gas liquid
chromatograph equipped with flame ionization detector (FID).

A-5 Apparatus
A-5.1 Gas Liquid Chromatograph (GLC)
Equipped with a FID and a printer-plotter cum- integrator. The suggested operating
parameters are given below which may be varied provide standardization is done:

Column – Glass packed 1.83m X 2(I.D) mm Packed with 3% OV-101 on

Gas Chrom Q (80 – 100 mesh) Condition at 240°C for at least 24 h
Using carrier gas at about 20 ml/min
.
Temperature Condition–
Column oven - 180°C
Injection port - 250°C
Detector - 250°C
Gas Flow-Carrier gas (Nitrogen) - 25 ml/minute
Hydrogen - 30 ml/ minute
Air -300 ml/minute

A-5.2 Volumetric Flask - 50-ml capacity.

A-5.3 Micro Syringe - 2 µL

A-6 Reagents
A-6.1 Acetone- AR grade.
A-6.2 Metolachlor Reference Standard-of known purity.
A-6.3 Internal Standard-Di pentyl pthalate, AR grade.

A-7 Procedure
A-7.1 Preparation of Internal Standard Solution
Weigh about 4 gm of Dipentyl pthalate into a 1000-ml volumetric flask and make up the
volume to the mark with Acetone.
Prepared By Approved & Issued By

Technical Manager Quality Manager

 Meghmani Industries Limited
Quality Control Laboratory, Unit-2
Plot No. 27, Phase–1, GIDC Industrial Estate, Vatva, Ahmedabad,
Pin–382 445, Gujarat, India Tel. No.  +91–81550 02071, Fax No.  +91–79–2589 3495
E Mail  qc@mil2.meghmanidyes.com, Web  www.meghmanidyes.com

01-09-
Doc. No. W/OPN/70 ANALYSIS METHOD FOR Date of Issue
2016
Issue No. 01 METOLACHLOR TECHNICAL Page No. 2 of 2

A-7.2 Preparation of Reference Standard Solution

Weigh 0.20 gm of the reference standard Metolachlor into a 50-ml volumetric flask and
add 50 ml of the internal standard solution (see A-7.1). and shake well for 10 Min.

A-7.3 Preparation of Sample Solution

Weigh a quantity of sample so as to contain0.20 gm of the reference standard Metolachlor
into a 50-ml volumetric flask and add 50 ml of the internal standard solution(see A-7.1). and
shake well for 10 Min.

A-8 Estimation
Inject 2 µl of the reference standard solution into the gas chromatograph followed by the
sample solution with the help of syringe and measure the peak areas of standard and
Sample solutions. Compute the percentage of Metolachlor in sample.

A-9 Calculation
M 1× A 1 × A 3
Metolachlor content, percent by mass = ×P
M 2× A2× A4
Where,
M1=mass in g of reference standard Metolachlor,
A1= peak area of internal standard in the standard solution,
A3= peak area of Metolachlor in the sample solution,
M2= mass in gm of sample taken for the test,
A2= peak area of Metolachlor in the standard solution,
A4= peak area of internal sample solution, and
P= percent purity of Metolachlor reference standard.

Reference : CIPAC D 400/TC/M

Prepared By Approved & Issued By

Technical Manager Quality Manager