Professional Documents
Culture Documents
and
Environmental Plant
Physiology
KRReddy@pss.msstate.edu
Department of Plant and Soil Sciences
Mississippi State University
Mississippi State, MS
What is Remote Sensing?
Remote Sensing
0.7
Internal tissue strongly
reflective, biomass content COTTON
0.6
Moisture content
of vegetation,
0.5
contrast between
vegetation types
Reflectance
0.4
0.3
Chlorophyll
0.2
0.1
Phenolics
0
400 800 1200 1600 2000 2400
Wavelength (nm)
Peep through Spectrally
Cotton Leaves and Spectral Images
589 700
800
760
What causes the reflectance to change?
Absorption Spectra of Chlorophyll a, b
Beta Carotene, and other pigments
Reflectance and leaf structure
WAX LAYER
UPPER EPIDERMIS
PALISADE LAYER
CHLOROPLASTS
MESOPHYLL CELLS
SUB-STOMATAL
CAVITY
GUARD CELLS
LOWER EPIDERMIS
What
What can
can we
we measure?
measure?
Multi-spectral
or
Hyperspectral
What is Multispectral Imaging?
quantitative results.
• Multispectral imaging
Landsat TM
reflectance
systems under sample the
spectrum. AVIRIS
• Identification of vegetation
types, rock and soil types 1 2 3
Landsat 4, 5 7 bands @ 30 m
GER 63 bands @ 10 m
SPACE
AERIAL
VAR
(processed data)
Remote Sensing Includes ….
• Satellite imagery
• Aerial photography
• Radar and Lidar
• Tractor-mounted sensors
• Hand-held instruments or sensors
Sensors
0.6 CORN
SORGHUM
0.5 SOYBEAN
Reflectance
COTTON
0.4
0.3
0.2
0.1
0
400 900 1400 1900 2400 2900
Wavelength (mm)
Remote Sensing
Examples:
• Topography
• Tree height
• Canopy water content
• Vegetation type
• Biomass by component
• Canopy structure
Remote Sensing - LIDAR
http://www.csc.noaa.gov/products/sccoasts/html/tutlid.htm
Large area
Inventories
Regional Crop
Mapping
Remote Sensing – Agriculture
Today, We do Several Things
Crop maps for the 6 counties in Hungary derived from multitemporal high-resolution satellite data
(Landsat TM and IRS-1C LISS III.) from the early May-August period of 1997.
Estimating Crop Yield in Hungary
Winter wheat yield forecast for the 6 counties in Hungary using our developed
Landsat/IRS + NOAA AVHRR model.
Winter wheat yields Winter barley yields
per county and Hungary per county and Hungary
COSH
Central Statistical Office,
Hungary
1.00
0.75
Reflectance
0.50
Corn
Sorghum
0.25
Cotton
Soybean
0.00
500 1000 1500 2000 2500
Wavelength, nm
Remote Sensing and Weed Species
0.6 25
Sesbania
0.3
10
0.2
5
0.1
0.0 0
400 900 1400 1900 2400 Ipomea Cocklebur Prickly Sesbania Sicklepod
Sida
Band 2
Band 1
1. Image space: Display of data in relation to one another in a geometric or geographic
sense, and provides a way to associate each pixel with a location on the ground.
2. Spectral space: High degree of spectral detail or response to the type of material on
the ground (grass, trees, soil, water etc.).
3. Feature space: Provides pattern recognition.
Interpretation and Analysis – Interpretation
Analog Digital
1. Tone
2. Shape 1. Preprocessing
3. Size 2. Image Enhancement
4. Pattern 3. Image Transformation
5. Texture 4. Image Classification and Analysis
6. Shadow
7. Association
Interpretation and Analysis – Analysis
1) Reconnaissance mapping:
• forest cover type discrimination
• agroforestry mapping
2) Commercial forestry:
• clear cut mapping / regeneration assessment
• burn delineation
• infrastructure mapping / operations support
• forest inventory
• biomass estimation
• species inventory
3) Environmental monitoring
• deforestation (rainforest, mangrove colonies)
• species inventory
• watershed protection (riparian strips)
• coastal protection (mangrove forests)
• forest health and vigor
Geological Applications
1. ice concentration
2. ice type / age /motion
3. iceberg detection and tracking
4. surface topography
5. tactical identification of leads: navigation: safe shipping routes/rescue
6. ice condition (state of decay)
7. historical ice and iceberg conditions and dynamics for planning purposes
8. wildlife habitat
9. pollution monitoring
10. meteorological / global change research
Land Use Applications
1.planimetry
2.digital elevation models (DEM's)
3.baseline thematic mapping / topographic mapping
Ocean and Coastal Monitoring Applications
• Ocean pattern identification: • Oil spill
• currents, regional circulation • mapping and predicting oil-spill extent
patterns, shears and drift
• frontal zones, internal waves, • strategic support for oil spill
gravity waves, eddies, emergency response decisions
upwelling zones, shallow water • identification of natural oil seepage
bathymetry areas for exploration
• Storm forecasting • Shipping
• wind and wave retrieval • navigation routing
• Fish stock and marine mammal • traffic density studies
assessment • operational fisheries surveillance
• water temperature monitoring • near-shore bathymetry mapping
• water quality • Intertidal zone
• ocean productivity, • tidal and storm effects
phytoplankton concentration • delineation of the land /water interface
and drift • mapping shoreline features / beach
• aquaculture inventory and dynamics
monitoring • coastal vegetation mapping
• human activity / impact
Need More Visit Remote sensing tutorial sites
http://rst.gsfc.nasa.gov/
http://www.vtt.fi/aut/rs/virtual/
http://www.remotesensing.org/
http://www.research.umbc.edu/~tbenja1/
http://dynamo.ecn.purdue.edu/~biehl/MultiSpec
http://www.cla.sc.edu/GEOG/rslab/images.html
http://www.cla.sc.edu/geog/rslab/rsccnew/fmod1.html
http://www.ccrs.nrcan.gc.ca/ccrs/eduref/tutorial/tutore.html
http://www.agso.gov.au/education/remote_sensing/TOC.html
Early
Early Sensitive
Sensitive Indication
Indication of
of Particular
Particular Stresses
Stresses
Mild
Intensity of stress
Severe
Development of Stress
Remote Sensing environmental limitation of
crop growth, development and yield
0.6
360 ppm CO2
0.5 720 ppm CO2
Reflectance
0.4
0.3
0.2
0.1
0
0 500 1000 1500 2000 2500 3000
Wavelength (nm)
Remote Sensing environmental limitation of
crop growth, development and yield
20 25 30 35 40
Environment Factors
Temperature:
¾ Strongly Affects:
-- Phenology
-- Vegetative growth, LAI, LAD
-- Fruit Growth and Retention
-- Respiration
-- Water-loss and Water-Use
¾ Moderately Affects:
-- Photosynthesis on a canopy basis
Remote Sensing and Environment
Thermal Imagery
http://wwwghcc.msfc.nasa.gov/irgrp/lst_goes_UHEI.html
Remote Sensing and Environment
Thermal Imagery
Thermal image of a cotton canopy that was part of a water and nitrogen
study in Arizona. Blues and greens represent lower temperatures than
yellow and orange. The image was acquired with a thermal scanner on
board a helicopter. Most of the blue rectangles (plots) in the image
correspond to high water treatments.
Relationships between NDVI and plant height, the number of nodes,
LAI or biomass
1.0
0.9 0N
50 N
0.8 100 N
150 N
0.7
NDVI
0.6
0.5 -0.030x
Y = 0.944(1-e ) Y = 1.48(1-e-0.057x)
0.4 R2 = 0.99 R2 = 0.97
0.3
0.2
0 20 40 60 80 100 0 5 10 15 20
-1
Plant Height (cm) MSN (No. plant )
1.0
Col 10 vs Col 7
0.9 Col 10 vs Col 7
x column 2 vs y column 2
0.8 Col 14 vs Col 8
Col 18 vs Col 8
0.7 Col 22 vs Col 8
NDVI
0.6
0.5
R2 = 0.97 R2 = 0.94
0.3
0.2
0 1 2 3 0 200 400 600 800
Stem GR
Vegetative
Sensitivity to water deficit
Boll GR
Sequence of events
Remote Sensing – Water Stress
Remote Sensing – Water Stress
Plant water status
0.0 12
-0.2
10
-0.4
8
-0.6
-0.8 6
-1.0
4
-1.2
2
-1.4
-1.6 0
0:00 4:00 8:00 12:00 16:00 20:00 0:00
Time of Day
Leaf water potential
-0.5
Leaf water potential (MPa)
y = -0.0017x - 0.0614
-1.0
R2 = 0.9976
y = -0.003x + 0.1888
-2.0
R2 = 0.9818
-2.5
-3.0
480
8 540
9 600
10 660
11 720
12 780
13 840
14
Time of day (h)
Leaf water potential vs. Photosynthesis
50
45
Photosynthesis (µmol m-2 s-1)
40
35
30
25
20
P n = 39.29-0.097*0.7732 LW P
15 R 2 = 0.78
P < 0.001
10
0
-2.5 -2.0 -1.5 -1.0 -0.5
0.7
0.6
0.5
Reflectance
0.4
0.3
0.2
0.1
0.0
0 500 1000 1500 2000 2500 3000
Wavelength (nm)
Hyperspectral reflectance ratio vs. Leaf water potential
1.00
Reflectance Ratio (1689/1657)
0.99
0.99
y = 0.005x + 0.9954
R2 = 0.6636
0.98
0.98
-2.5 -2.0 -1.5 -1.0 -0.5
-0.5
Predicted LWP (MPa)
-1.0
-1.5
-2.0
-3.0
-3.5
-3.5 -3.0 -2.5 -2.0 -1.5 -1.0 -0.5 0.0
Observed LWP (MPa)
Photosynthesis prediction using LWP and hyperspectral ratio
34
30
28
26
24
24 26 28 30 32 34
Maize
Sorghum
2 Cotton
Sunflower
Rice
Soybean
1
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5
Leaf Nitrogen, g m-2
Environment - Nitrogen
1.2
Environmental Productivity
1.0
Idices for Nitrogen
Photosynthesis
0.6
0.2
Leaf Growth
0.0
1.00 1.25 1.50 1.75 2.00 2.25 2.50
Leaf Nitrogen, g m-2 leaf area
•Cotton
Cotton Nitrogen Nitrogenand
- Physiology Experiment
Spectral Properties
•Physiology•Leaf
andReflectance
Spectral Properties
0.6
•0.6
Control
•Control
Nitrogen
•Nitrogen deficit
•-•Deficient
•Reflectance
Reflectance
0.4
•0.4
0.2
•0.2
0.0
•0.0
•500
500 •1000
1000 •1500
1500 •2000
2000 •2500
2500
•nm nm
Wavelength,
Cotton Leaf N Concentration vs. R517/R413
(Field N and Pix Studies)
2.5
2001 2001
N study
2.0 MC study
1.5
1.0
Y = -0.2784X + 2.0559
R517/R413
Y = -0.0176X + 1.9947
r2 = 0.78, n = 120 r2 = 0.69, n = 120
0.5
2002 2002
2.0
1.5
1.0
Y = -0.0205X + 2.2225 Y = -0.3303X + 2.3375
r2 = 0.65, n = 156 r2 = 0.73, n = 156
0.5
0 10 20 30 40 50 60 1.0 2.0 3.0 4.0
0.4
2001 2002
R551/R915
0.3
0.2 N study
MC study
Y = -0.0043X + 0.4549 Y = -0.00029X + 0.4821
2 2
r = 0.67 r = 0.83
0.1
2001 2002
0.6
R708/R915
0.5
0.4
50 50
100% N
Leaf Pn (µmol m-2 s-1)
20% N
40 0% N 40
0% NL
30 30
20 20
Y = 7.068X + 11.62 Y = 21.601X + 8.448
R2 = 0.551 R2 = 0.793
10 10
1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.4 0.6 0.8 1 1.2 1.4 1.6
•0.999 Chl. a
•0.9998 Chl. b
•0.998
•R756/R758
•R764/R765
• 0% N •0.9996
•0.997 • 0% NL
• 20% N
•0.9994
•0.996 •100% N
Reflectance Ratio
•0.9992
•0.995 •y = -9E-05x + 0.9999 •y = -0.0001x + 1.0001
•2 •2
•R • = 0.5978
•R • = 0.6128
•0.994 •0.9990
•0 •10 •20 •30 •40 •50 •2 •4 •6 •8
•1.000
•0.9990
Total Chl. Carotenoids
•0.999
•0.9980
•R756/R758
•R756/R759
•0.998
•0.9970
•0.997
•0.9960
•1.15 •0.80
•100% N
• 20% N •0.70
•1.10
• 0% N
R550/R1280
• 0% NL
R505/R645
•1.05 •0.60
•1.00 •0.50
•0.95 •0.40
Y = 0.0369X +0.8949 Y = -0.2168X + 0.7437
R2 = 0.750 R2 = 0.510
•0.90 •0.30
•0.0 •1.0 •2.0 •3.0 •4.0 •5.0 •6.0 •0.4 •0.6 •0.8 •1 •1.2 •1.4 •1.6
Leaf N (%) Leaf N (g m-2)
Sorghum Nitrogen Experiment
Physiology and Spectral Properties
Sorghum Leaf Chlorophyll or N vs. Reflectance Ratio
(Pot Study)
1.4 0.5
100% N (A) (B)
1.3 20% N
0% N 0.4
R1075/R735
R405/R715
1.2
0.3
1.1
0.2
1.0 Y = 0.0003 X + 1.0124 Y = 0.0047 X + 0.1591
r2 = 0.662 r2 = 0.676
0.9 0.1
200 350 500 650 800 15 25 35 45 55
Leaf chlorophyll (mg m-2) Leaf N (g kg-1)
Corn Leaf Chlorophyll or N vs. Reflectance Ratio
(SPAR Study)
1.0 1.08
0.8 1.04
R712/R1088
0.6 1.00
R575/R526
0.4 0.96
0.2 0.92
2 2
Y = -0.00086X + 0.845, r = 0.59 Y = -0.0017X + 1.016, r = 0.687
0.0 0.88
0 100 200 300 400 500 0 10 20 30 40 50
-2 -1
Chlorophyll (mg m ) Leaf N (g kg )
Nitrogen, Physiology and Spectral
Properties – Various Crops
N sensitive
Algorithm† N r2 Reference
Crop bands (nm)
Corn 555, 715 Chl = [(R712/R1088) – 0.845] ÷ (-0.00086) 36 0.59 Zhao et al., 2003a
Cotton 555, 710 Chl = [(R708/R915) – 0.722] ÷ (-0.00040) 156 0.76 Zhao et al., 2004b
Sorghum 555, 715 Chl = [(R1075/R735) – 1.012] ÷ (0.00030) 21 0.66 Zhao et al., 2004c
† Chl = chlorophyll (mg m-2); N = leaf nitrogen (g kg-1); R = reflectance and subscript values are the wavelengths (nm).
Nutrient Deficiency – Potassium
Environment Factors
¾ Moderately Affects:
-- Photosynthesis
-- Stem growth
Environment - Nutrients
Potassium - Cotton Growth and Development
Environmental Productivity Indices
1.6
Environmental Productivity Indices
1.4
for Growth and Development
0.0
-0.2
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5
Leaf Potassium, %
Cotton Potassium Experiment
Physiology and Spectral Properties
70
60 Sufficient K
Medium K
50 Low K
Reflectance, %
40
30
20
10
1.0
0.8
UV-B index
Node addition
0.6 2 2
y = -0.0003x + 0.0014x + 0.9997 R = 0.4619
Le af are a e xpansion
2 2
0.4 y = -0.0015x + 0.0102x + 0.9914 R = 0.8136
Ste m e longation
2 2
0.2
y = -0.0023x + 0.0105x + 0.9926 R = 0.9331
0.0
-4 0 4 8 12 16 20
UV-B treatments (kJ m-2 d-1)
Environment Factors
Ultraviolet-B Radiation:
¾ Strongly Affects:
-- Photosynthesis
-- Stem growth
¾ Moderately Affects:
-- Leaf growth
-- Leaf aging
¾ No Effects:
-- Phenology
Hyperspectral Reflectances of Cotton Leaves Exposed
to Different UV-B and CO 2 Treatments
0.6
0.2
0.1
Reflectance
0
0 500 1000 1500 2000 2500 3000
0.6
0.5
720 ppm
0.4
0.3
0.2
0.1
0
0 500 1000 1500 2000 2500 3000
Wavelength(nm)
Breakdown of spectral reflectances of cotton leaves into specific waveband regions
with known properties exposed to different UV-B and CO2 treatments
0.20 0.60
0.15
Chlorophyll reflectance 0.55 Internal tissue reflectance
0.10 0.50
0.05 0.45
Reflectance
0.00 0.40
500 525 550 575 600 625 650 675 700 750 850 950 1050 1150 1250
0.35 0.20
0.30 0.15
0.25 0.10
Leaf water reflectance
0.20 0.05
1550 1600 1650 1700 1750 1800 1850 2000 2050 2100 2150 2200 2250 2300 2350 2400
Wavelength (nm)
Deviation of Spectral Reflectances of Cotton Leaves
Exposed to Different UV-B Treatments from Control
at Squaring and Flowering Stages
0.08 0.08
At squaring At flowering
control (0 kJ UVB) treatment
0.04 8 kJ UVB
0.04
16 kJ UVB
0.02 0.02
0.00 0.00
-0.02 -0.02
0 kJ UVB
-0.04 -0.04
8 kJ UVB
-0.06 -0.06
16 kJ UVB
-0.08 -0.08
400 900 1400 1900 2400 400 900 1400 1900 2400
Wavelength (nm)
Remote Sensing environmental limitation of
crop growth, development and yield
¾ Slightly Affects:
-- Photosynthesis
Mepiquat Chloride (PIX) - Growth
EPI Factors
1.2
1.0
Leaf growth
PIX and EPI Indices
0.8
Photosynthesis
0.6
0.4
Stem growth
0.2
0.0
0.00 0.01 0.02 0.03
Mepiquat Chloride, mg g-1 dry weight
Mepiquat Chloride (PIX) and
Cotton Growth and Remote Sensing
80
-1
MC Application Rate (g a.i. ha ) (A)
70
0
60
25
50
Reflectance (%)
100
50
40
30
20
10
0
(B)
2
-2
-4
-6
-8
-10
-12
300 400 500 600 700 800 900 1000
Wavelength (nm)
Mepiquat Chloride (PIX) and
Cotton Growth and Remote Sensing
• As expected, application of MC decreased plant
growth, increased leaf chlorophyll concentration,
and decreased leaf reflectance.
• Reflectance values at 420, 545, 810, and 935 nm
separated MC-treated plants from untreated plants
under favorable growth conditions, but we were
unable to distinguish the different application rates
of MC.
• Forward stepwise regression and discriminant
analysis suggested that changes in leaf reflectance
from MC application were due to increased
chlorophyll and nitrogen concentrations.
Remote Sensing environmental limitation of
crop growth, development and yield
0.6
360 ppm CO2
0.5 720 ppm CO2
Reflectance
0.4
0.3
0.2
0.1
0
0 500 1000 1500 2000 2500 3000
Wavelength (nm)
Remote Sensing and Environment
Thermal Imagery
Thermal image of a cotton canopy that was part of a water and nitrogen
study in Arizona. Blues and greens represent lower temperatures than
yellow and orange. The image was acquired with a thermal scanner on
board a helicopter. Most of the blue rectangles (plots) in the image
correspond to high water treatments.
Hyperspectral reflectance ratio vs. Leaf water potential
1.00
Reflectance Ratio (1689/1657)
0.99
0.99
y = 0.005x + 0.9954
R2 = 0.6636
0.98
0.98
-2.5 -2.0 -1.5 -1.0 -0.5
N sensitive
Algorithm† N r2 Reference
Crop bands (nm)
Corn 555, 715 Chl = [(R712/R1088) – 0.845] ÷ (-0.00086) 36 0.59 Zhao et al., 2003a
Cotton 555, 710 Chl = [(R708/R915) – 0.722] ÷ (-0.00040) 156 0.76 Zhao et al., 2004b
Sorghum 555, 715 Chl = [(R1075/R735) – 1.012] ÷ (0.00030) 21 0.66 Zhao et al., 2004c
† Chl = chlorophyll (mg m-2); N = leaf nitrogen (g kg-1); R = reflectance and subscript values are the wavelengths (nm).
Cotton Potassium Experiment
Physiology and Spectral Properties
70
60 Sufficient K
Medium K
50 Low K
Reflectance, %
40
30
20
10
0.2
0.1
Reflectance
0
0 500 1000 1500 2000 2500 3000
0.6
0.5
720 ppm
0.4
0.3
0.2
0.1
0
0 500 1000 1500 2000 2500 3000
Wavelength(nm)
Remote Sensing and Insect Infestation
Genotype Study North Farm, MSU
Pix Plots
Irrig.
Plots
Nitrogen Plots
9 9.0
8 8.0
7 7.0
N IR (R 7 5 0 /R 5 5 0 )
N IR (R 7 5 0 /R 5 5 0 )
6 6.0
5 5.0
4 4.0
3 3.0
8.0
20
7.0
NIR (R750/R650)
NIR (R750/R550)
6.0
15
5.0
4.0
10
3.0
NDVI [(R935-R661)/R935+R661)]
0.95
0.90
25
0.85
NIR (R935/R661)
20 0.80
0.75
15
0.70
10 0.65
X Y =0.9X/(12.34+X)
Y =21.6(1-0.99 ) 0.60
R2 = 0.63
5 R2 = 0.76
0.55
0 0.50
0 100 200 300 400 500 600 700 800 900 1000 0 100 200 300 400 500 600 700 800 900 1000
7
20
NIR (R750/R650)
NIR (R750/R550)
6
15
10
4
5 Y = -39.9+54.9(1-0.22X) 3
Y = -5.65+12.27(1-0.21X)
R2 = 0.68 R2 = 0.56
0 2
0 1 2 3 4 5 6 0 1 2 3 4 5 6
30 0.95
NDVI [(R935-R661)/(R935+R661)]
0.90
25
0.85
NIR (R935/R661)
20 0.80
0.75
15
0.70
10 0.65
X
Y = -50.0+71.0(1-0.23 ) 0.60 Y = -0.359+1.25(1-0.156X)
5 R2 = 0.70 R2 = 0.62
0.55
0 0.50
0 1 2 3 4 5 6 0 1 2 3 4 5 6
8.0
20
7.0
NIR (R750/R650)
NIR (R750/R550)
6.0
15
5.0
4.0
10
3.0
NDVI [(R935-R661)/R935+R661)]
0.90
25
0.85
NIR (R935/R661)
20 0.80
0.75
15
0.70
10 0.65
X
Y = -1.4+25.5(1-0.51 ) 0.60
Y = 0.59+0.32(1-0.0.44X)
5 R2 = 0.72 R2 = 0.67
0.55
0 0.50
0 1 2 3 4 5 6 0 1 2 3 4 5 6
80 150 N
60 10
40
20
0 0
-2
4
600
Lead Area Index
3
400
2
200
1
0
0
20 40 60 80 20 40 60 80
0.95
0.90
0.85
NDVI
0.80
0N
50 N
100 N
0.75 150 N
0.70
40 60 80 100
0.9 0N
50 N
0.8 100 N
150 N
0.7
NDVI
0.6
0.5 -0.030x
Y = 0.944(1-e ) Y = 1.48(1-e-0.057x)
0.4 R2 = 0.99 R2 = 0.97
0.3
0.2
0 20 40 60 80 100 0 5 10 15 20
-1
Plant Height (cm) MSN (No. plant )
1.0
Col 10 vs Col 7
0.9 Col 10 vs Col 7
x column 2 vs y column 2
0.8 Col 14 vs Col 8
Col 18 vs Col 8
0.7 Col 22 vs Col 8
NDVI
0.6
0.5
R2 = 0.97 R2 = 0.94
0.3
0.2
0 1 2 3 0 200 400 600 800
140 20
100
14
80 0N 12
50 N
60 100 N 10
150 N 8
40
6
20
4
0 2
5
Y = e 7.25X
600 R2 = 0.95
4
Y = 2.35-1.22X+1.54X2
R2 = 0.99
Biomass (g m-2)
200
1
0 0
-1
0.2 0.4 0.6 0.8 1.0 0.2 0.4 0.6 0.8 1.0
NDVI NDVI
What’s Next?
Remote Sensing (RS):
Provide spatial variable data for crop health and
yield, and soil conditions, and some predictive
capabilities.
Crop Simulation Models and Decision Support
Systems(CSM-DSS):
Provide predictive capabilities and verification of
RS features.
RS and CSM-DSS:
Deliver site-specific input management or
optimization.