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Body Tube
This part supports the eyepiece and objectives. It is critical that the tube be constructed so that these optics share a common axis. Most 20th century scopes with body tubes (i.e., not modular) are designed for a mechanical tube of either 160 mm, or 170 mm. Mechanical tube length is the distance from the top of the eyepiece tube to bottom of the Society threaded objective holder.
Condenser
This probably the most overlooked microscope component. Very few people recognize the importance of a good quality, properly adjusted condenser, including many professional microscopists. It is a vital part of the illumination system, and is designed to collect, control and concentrate light from the lamp onto the specimen. As with objectives, the optical elements can introduce a variety of aberrations which are corrected to varying degrees, depending on the type of condenser one is using. Condensers (as well as filters and objectives) are available to provide specialized, contrast enhancing illumination such as darkfield, Rheinberg, polarization, differential interference contrast, and phase contrast. If you are using a basic bright field condenser, with a simple lamp or daylight (cloudy sky - not direct sunlight) ensure that it is racked all the way up, so that the top element is almost touching the under side of the slide. Do not move the condenser up and down to adjust light intensity. To ensure maximum contrast and resolution remove the eyepiece, look into the tube and open the condenser iris until about two thirds of the objectives rear element is filled with light. As with moving the condenser, the iris diaphragm (aperture iris) should not be used to control field brightness. If the field of view is too bright use a neutral density filter, or lamp rheostat to correct this problem. Microscopes with internal or external lamps that include a field diaphragm and adjustable condensing system can be set up for K hler illumination. This technique provides the best possible illumination.
Draw tube
At one time "all good instruments" had a body tube equipped with an inner sliding draw tube. This tube enabled users to the adjust the mechanical tube length when certain accessories were screwed on between the eyepiece and objective, or when using objectives designed for longer mechanical tube lengths. Today's microscopes no longer have or need such a device.
Eyepiece (Ocular)
Eyepieces are produced in a number of different designs. For viewing purposes, the Kellner design is preferable. The top element is an achromatic doublet, and it provides a large, flat, well corrected field of view, compared to the basic Huygenian design (often supplied with 1950's and earlier stands). An additional bonus is the higher eye point, which makes viewing more pleasurable (very high eye point models are available for spectacle wears). Although some eyepieces are designed to complement a specific series of objectives (compensating eyepieces), for the most part, they are interchangeable among manufactures. The vast majority are standardized at either 23 mm or 30 mm outside diameter. An eyepiece cannot improve the inherent resolution of the image formed by an objective, but one of poor design can degrade it.
Eyepiece tube
A fixed tube into which the eyepiece is inserted. For mainstream, "professional" scopes, the inside diameter is either 23 mm or 30 mm.
Foot (base)
It rests on the bench top and supports the stage and body of the microscope, and in many cases also houses the lamp. A well designed base will ensure that the image does not dance about during focusing, or while manipulating the specimen. There are a vast number of different base designs.
Limb (arm)
The arm is attached to the foot (in scopes without an inclined viewing head by means of an "inclination joint") and supports the body tube. The shape of the arm, and the way in which the body is attached, are often used to illustrate the history of the microscope's development. Today most student and research stands (older term for a microscope without optics) have very liner, computer designed arms far different in appearance from the one shown in the photograph (which is a classical "Lister limb" - made about 1930). In such modern stands the "body tube" has been replaced by two removal parts, a viewing head and an objective changer, with the top end of the arm forming the middle section. This type of arm is very strong and can better support additional equipment, such as video cameras. (Also, as the stage, not the arm moves during set-up, there is no longer any concern about additional weight causing the body tube to drift downward, and out off focus.)
Objective
This, together with the condenser, is the microscope! If you have a poor quality objective nothing you can do will change this. It is much better to start with two good quality objectives then four mediocre ones. While modern, fixed focus (not infinite focus) objectives have a standardized "adjustment length" (so called "DIN standard"), and are Society threaded, the degree of optical correction varies. In many cases the barrel of the objective is engraved with information on its optical characteristics. Objective lenses are very tiny and as a result great care is need to form and assemble such lens systems. This generally translates into time, which in turn translates to cost. Furthermore, it is of paramount importance that manufactures of such equipment maintain a very high level of quality control. Keep this in mind when shopping for objectives (complete microscopes as well). An important point to consider when buying older objectives is compatibility. Before DIN was universally adopted by main stream manufactures objectives were generally shorter, typically having an adjustment distance of 37 mm (measured from the shoulder of the attached objective to the plane of focus in the specimen). By contrast DIN objectives are much longer, with an adjustment distance of 45 mm. (The longer barrel provides more room for wider lens combinations needed to improve field-of-view, and field flatness.) So what does all this mean? Combining long and short barrel objectives destroys one of the unique features of 20th century microscopes - parfocality. A revolving nosepiece permits rapid changeover between objectives. In practical terms it is essential that the focus of the image be preserved during the change of objectives. Parfocal objectives allow this to happen (at worse only slight refocusing is required). If you mix DIN and short barrel objectives you will be constantly refocusing - or you will not be able to focus at all if the stand is designed for 37 mm objectives. Therefore best not to mix short barrel and DIN objectives. As the difference in length between the two types of objectives is quite significant, they are relatively easy to distinguish (always measure from the top of the objective shoulder to the surface of the front element). As a rough rule, a "short barrel" objective will always be less than 37 mm in length, a DIN objective will always more than 37 mm (10x and higher), powers above 40x almost 45 mm.
Stage clips
These are the basic stage slide holders. Supplied in pairs, they are adequate for general slide manipulation up to a maximum of 400x (if properly adjusted, which can be tricky). In the hands of a skilled operator a good pair can serve very well in this magnification range. However, nothing can beat a well made "mechanical stage". (If your scope lack clips elastic bands may do in a pinch.)
Stage
This is the platform or "stage" that supports the specimen (which are typically mounted on glass slides). To do this job properly it must be perfectly perpendicular to the optical axis, dead flat and of adequate size. A microscope with a dinged or out of line stage should be avoid. As mentioned above mechanical stages are often supplied. They may be integrated into the stage itself (with the stage deck moving in one axis rather than the slide holder) or they can be attached. Either way they make the life of a microscopist more enjoyable. However, as movement as well as size are magnified when using a microscope, they must be well made and in be maintained in top shape. This is another item to be scrutinized under viewing conditions when shopping for a scope. As with the focus controls, movement must be smooth and backlash free.