Name: Trillanes, Cristian Kenneth L.

Date: March 1, 2022

Course, Section & Set: BSN – 1B [ Set B ]

Exercise 1
FAMILIARIZATION WITH THE EQUIPMENT AND MATERIALS IN
MICROBIOLOGY LABORATORY

Familiarization with the different materials and equipment and their uses/functions is important to aid you,
the students, in the performance of the different laboratory exercises in microbiology. This will provide you with
the confidence necessary in doing the activities. A v ery functional microbiology laboratory should be furnished
with the following equipment/apparatuses and materials. At the end of the activity, the students are expected to
Identify and describe the functions of the different equipment, materials and apparatuses used in the
microbiology laboratory and be able to determine the principles behind and illustrate some of them.

A. Glassware, Plasticware and

Brief Description Function/Use
Tools
1. Screw-capped tube/Test tube  A clear glass with
cap (screw-
capped) or without  Test tubes are widely
(test tube) that is used by scientist such as
longer than it is chemist and biologist to
wide. handle, mix, or transfer
 Aka culture tube or chemicals or solutions.
sample tube.  Screw-capped test tubes
 It is glass tube that are used for storage
has convenient and/or transport of
sizes that suit for a samples.
variety of
application.

2. Petri dish

 Petri dish is used to hold

 A small shallow
growth nedium in which
dish made of glass
cells can be culture.
or plastic.
 Cells of bacteria and fungi
 It has a loose
are originally being
cover
cultured here

3. Pipette  Slender tube  Used for transferring or

measuring out small
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 attached to a bulb.
 A dropper-like tool quantities such as liquid
which some has substances.
measurement on it

4. Beaker

 A cylindrical
vessel, usually of  It is used as reaction
glass, with a flat container and use to hold
bottom liquid or solid samples.
 A sometimes  Beakers are used also for
narrow and has a preparing reagent solution
small beak at the
lips

5. Erlenmeyer flask

 It is a flask that
 Usually, Erlenmeyer flask
has a wide and flat
is used for mixing
base, narrow neck
chemicals.
and conical form.
 In microbiology, it is used
 It has
for the preparation of
measurements
microbial cultures
too.

6. Graduated cylinder

 AKA measuring or
mixing cylinder.
 Most precise  Commonly used to
volume measuring measure the volume of a
tool. liquid substances.
 It has narrow
cylindrical shape.

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7. Medium bottle
8. Dilution bottle
9. Stirring rod
10.Bent glass rod or L-shaped
glass rod
 A glass or plastic  Medium bottles are used
multipurpose for storing industrial
container/bottle. laboratory chemicals and
 Graduated reagents.
container in  Container used for use
quadrilateral or with biological and
round in shape. pathological specimens.
 Sterile bottles with  Used for samlple dilution
cover just like of water, dairy products,
medium bottle. foods, and
pharmaceuticals prior to
microbiological testing.
 A glass stick or a
rod that is made of  Stirring rod is used to mix
solid glass that is chemicals.
like a drinking  Used also in transferring
straw with no hole substances in another
and has rounded container.
end.
 Bent glass rods  L-shaped glass rods are
are glass rods that used to spread the
are bent into an L- microbe-containing liquid
shape. equally on the plate.
 Sterile glass rods
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11.Glass slide

 Glass slides are used to

 A thin and flat hold objects for
piece of glass examination under a
microscope.

12.Cover slip

 A small square or
 It protects the microscope
circle thin glass
and prevents the slide
that is placed over
from drying out when it's
the specimen on
being examined
the glass slide.

13.Spatula or scoopula  A stainless-steel  Used for scrapping,

laboratory utensil transferring, or applying
like a spoon. powders and paste-like
chemicals.

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14.Test tube rack

 A laboratory
equipment with  Used to hold upright
holes. Made of multiple test tubes at the
wood, stainless same time
steel, or plastic

15.Forceps

 Nonlocking
 Forceps are used for
grasping tools look
grasping and holding
like scissors or
small objects
tweezers.

16.Wire loop
 A tool usually
made of platinum
 It is used to smear, streak,
or nichrome wire
or take an inoculum from,
in which the tip
a culture of
forms a small loop
microorganisms
with a diameter of
about 5 mm

17.Wire needle  A laboratory tool  An equipment used in the

that looks like wire field of microbiology to
loop. transfer and inoculate
 Can be disposable living microorganisms.
or reusable

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18. Rubber aspirator

 A laboratory tool  Used as suction to

that is bulb that transfer liquid substances
made of rubber. to another glassware

19.Wash bottle

 It is used to wash or rinse

 A laboratory tool
various pieces of
which is a
laboratory glassware or to
squeeze bottle
precisely fill volumetric
with a nozzle,
flasks and other flasks.

20.Alcohol lamp

 A laboratory tool
 Alcohol lam is used to
that is either glass
produce an open flame to
or metal container
act as a heating source.
with wick and a lid.

B. Apparatus

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1. Microscope

 It is an instrument that
 It is a laboratory
can be used to observe
apparatus that
small objects, even cells
produces enlarge
so that scientist can
images of objects.
detailly observe them.

2. Top loading balance

 It is among the
most common  This is commonly used to
types of weight weigh solid material when
measuring scales a precision of 0.1 g is
used in the adequate.
laboratory.

3. Analytical balance  This apparatus is  It is used in quantitative

highly sensitive chemical analysis, to
lab instruments determine the mass of
designed to solid objects, liquids,
accurately powders and granular
measure mass. substances.

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4. Autoclave

 It is used kill harmful

 AKA steam bacteria, viruses, fungi,
sterilizer. and spores on items or
 a machine that laboratory tools
use steam under
pressure.

5. Microwave oven

 An appliance that  It is used for annealing,

produces high- die-bond curing, drying or
frequency dehydrating, Polyimide
electromagnetic baking, sterilizing,
waves. evaporating.

6. Incubator

 A laboratory
apparatus is an  The temperature-
insulated box that controlled environment
provides supports growth of
temperature- microbiological or cell
controlled cultures.
environment

7. Drying  It is an apparatus  Drying oven is used for

Oven which is a type of evaporation, sterilization,
low-temperature remove moisture to dry
convection or the samples as quickly as
forced air oven. possible, temperature
testing, and for incubating
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 temperature sensitive
experiments.

8. Water bath

 A laboratory  A laboratory apparatus

apparatus made that is used to incubate
from a container samples at a constant
filled with heated temperature over a long
water. period of time.

9. Pipettor

 A laboratory
equipment which
 This instrument is used to
commonly
transport a measured
consists of a
volume of liquid, often as
narrow tube into
a media dispenser
which fluid is
drawn by suction.

10. Lab Blender/Homogenizer  It is an object-  Lab blender/homogenizer

breaking and is used to blend chemical
mixing apparatus or biological samples that
and commonly includes lysing of cells to
used with mortar form one homogenous
and pestle.
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 suspension

11. Vortex mixer

 It is used commonly in
 A simple
laboratories to mix small
blending/mixing
vials of liquid.
laboratory device.
 Used to suspend cells

12. Laminar air flow/biosafety cabinet

 It is designed to designed
to prevent contamination
 It is a carefully
of semiconductor wafers,
enclosed bench or
biological samples, or any
cabinet
particle sensitive
materials.

13. Pressure cooker  It is an apparatus  Used for sterilization

just like autoclave  Pressure cooker chamber
but flame/fire reaches temperatures
operated. high enough to kill
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 bacteria and mold spores

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