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CL-33 oup A6Ga

MT H01 - qpid Liqicd Bokon


on
AM 4)To obsswe ycsaubes CeuN
a packed calumm
uvad Re
Rou in

)To eatinatu oatuHc acid concentatin UN g o u c ano

3)o dnens hale mao balan ce pefos med en Paskao


Cunnn dalculate mad ans fRciend.
the cslumn
4) To Caladat heldup in
umn
5) To calculatt theoichca numbur h dtays n

PBraon qaadtien.
ubin ksem su
Ding
tho retial CHETP)
6)6 Glaulattight ainalend tto
the column.

PPARATOS 1BureHe 3) Convcal fosk 5Maang nds


2) Pipe L 4) Baku 6 Sepa tia unnel

CHEMICALS Sedium Hydstiee pellus


REQUIRED 2) Acetc add
3) MIBK M t ls@buty ketone)
H) PheaephHa leun

6) Oxalic acol sabt

A uas phse
&CHEMATIC Rofia
(Ve x) | VaX)

MMBk% Acutit aod Wglot Agti ac


Oxgawc Phage act (Ve.
PRIMCIPLES A C)Liqil-iquid ehsctr?
THEORY In LLE, cCmpenod in ed
ratvicad
( phasphag ) as
to
esgkc addina a deed DR poc. hio
ms a rns phose
hcien
which i nie
important duing the
The Conpono to be a a dad dunng
which o tu dasised congonand
Tha emaning i d
dpotwrs tans-fvd to hact

h
huing de proCes, 2 Cqurcd phase cve fer md
dituonce phose compesiti8nus makis t mass tansts o

dukuion asert dpands n

Lensity, wisOsihy, Kaactiniy,Tensien


acleekiity. ecovesebiGty

(te iotbuction CoetHda us


mloduR
how a cEmpond
DC gves a quavtifcone
dinsibz Beten aquOLs emaic ha
Aslul e0luti Qxhsac phage
mtfrau pha
DeubiKH
DC impiesghs
Chct
l t hesding n quesus plase

(E) Spacudien
tha
the difoonce duiy o S p eneuio betwean
phaces ick
a paxab Cempeob vay . t makid the
a cpn anfial .

hacitn (an Casued pd n )Cowcte-unent


The
2) C- urent

Caboey Liquiel dataey hon and, A


e m htem. In t
Mas ha fo lctren elK ,

caoued upb tha top of *ha con


Cavsier Capid w umead Tm (a bo tte.
Civ) aokad colunn echockon
xhecipn column l d wth M
Cowrdac bops linposed
ohane and to cluct aial midrg
pokng a wh CENti nuLa
pae
daonwsdy ? h dinpar
phaae ing uuavds to coalasce inf bk aue, bng
an inte e .

This itofate ust be maitaineo wouh a rtkel vale


fDo Cocs pendi peoue he hottam 6ukatipe.
k n as btfaic e hpmcking ust be med
b coti nueud pls t prewen oiopued pose pepa
seam instad oplaiy okiya must e l s
than Vg Coum olaau.

(V Kamse BattOnaatien

d calculau the numban khuesctira dgas


pooked celumn
-sm
- s l mj
lag E

umbe ctherercal s t qu(o


Xn nc

m= distibition codtiiant
E eacttn foctos nS/E)
HETP ( Jai Ad i velento thuorekical pla)
plat

HETP = Paokeing
n oh teoietical stag

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