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Histology Lab Practicals - AY 2021-2022
Histology Lab Practicals - AY 2021-2022
7. Identify tissue pointed. 9. Identify tissue where pointer lies. 11. Identify tissue.
1. Identify the structure pointed. 3. Identify tissue pointed.*Specimen – 5. Identify tissue in field.
- Simple cuboidal epi - Mucous CT - Simple squamous epi
- Nucleolus gallbladder - Lymphoid/Lymphatic CT
8. Give 2 examples. 10. Give its preponderant cell. 12. Give its specific name.
2. Give its main component. - Simple columnar plain epithelium 6. Give its preponderant fiber.
- Thyroid follicle & collecting tubules of - Fibroblast - Mesothelium
- rRNA 4. Give one characteristic of epithelium in - Type III collagen fiber
kidneys (liver cell is NOT an example even 13. What is actually stained? Intercellular
general.
though it is cuboidal) substance of the cell
- scanty intercellular substance;
- Rest on LP with BM in between;
- Avascular but well supplied w/ nerves;
- May undergo metaplasia;
- Tissue layer always has 1 free surface
exposed to environment
13. Identify tissue where pointer lies. 15. Identify tissue where pointer lies. 17. Identify structure at tip of pointer. 19. Identify tissue pointed. 21. Identify tissue where pointer lies. 23. Identify tissue where pointer lies.
- Mesenchymal CT - Stratified squamous epi - Reticular fiber - Transitional epithelium *specimen- trachea (L-section) - Pseudostratified columnar ciliated epi
14. Identify cell pointed. 16. Identify structure pointed. 18. Give the stain used. 20. What is other name of the tissue. - Adipose CT 24. Identify structure pointed.
- Undifferentiated mesenchymal cell - Connective tissue papillae - Silver nitrate - Urothelium 22. Give its preponderant fibers. - Basement membrane
- Type III & IV collagen fibers
2ND SHORT PRACTICAL
1. Identify the tissue 3. Identify tissue where pointer lies. 5. identify tissue pointed (black arrow). 7. Identify tissue where pointer lies. 9. Identify epithelium. 11. Identify tissue.
-long Bone Tissue - Dense elastic CT (cross-section) - Simple squamous epi (mesothelium) - Hyaline cartilage - Simple columnar plain epi - Smooth muscle tissue (cross-section)
2. Identify structure pointed 4. Give its preponderant fiber. 6. Give one general characteristic of 8. Why is the matrix homogeneous? 10. Give 2 examples: 12. Give its structural and functional unit.
- Cementing Line - Elastic fiber epithelium. - Because the matrix & collagen fibers - Stomach, small/ large intestine, gall -Smooth Muscle fibers
5. Organ examples - Avascular, Scanty, Rests on LP, have the same refractive index bladder
-ligamentum nuchae metaplasia, 1 free surface exposed to
-Aorta environment
15. Identify tissue in the field. 17. Identify the dark wavy structure. 19. Identify tissue above the pointer. 21. identify tissue where pointer lies. 23. Identify cell pointed.
13. Identify epithelium.
- Elastic cartilage - Elastic fiber - Stratified squamous epi - Cardiac muscle (long-section) - Osteocyte
- Pseudostratified columnar plain epi
16. Identify part where pointer lies. 18. Give the stain used. 20. Identify tissue below the pointer. 22. Give its distinguishing structural 24. Give its function.
14. Give its distinguishing structural
- Area of fatty degeneration - Resorcin - fuchsin stain - Lymphoid CT characteristic - Maintenance of the integrity of the
characteristic.
- Presence of intercalated discs matrix, maintain bone metabolism
- Nuclei lies at different levels
MIDTERMS PRACTICAL EXAM
3. Identify where pointer lies. 5. Identify tissue in the field. 7. Identify tissue where pointer lies. 9. Identify epithelium. 11. Identify tissue.
1. Identify tissue.
- Dense elastic CT - Simple squamous epi (Mesentery) - Hyaline cartilage - Pseudostratified columnar plain epi - Fibrocartilage
- Bone tissue
4. Give two (2) examples. 6. Give one (1) general characteristic of 8. What is responsible for its 10. Give two (2) examples. 12. Give its distinguishing structural
2. What is responsible for its strength and
- Ligamentum nuchae & flava, true vocal epithelium. metachromatic staining? - Vas deferens, seminal vesicle characteristic.
resiliency?
cords - Scanty, avascular but well supplied w - Chondroitin sulfate & Keratan sulfate - Herring bone arrangement
- Organic components
nerves , metaplasia
13. Identify structure at tip of pointer. 15. Identify tissue in field. 17. Identify organ. 19. Identify tissue above the pointer. 21. Identify tissue where pointer lies. 23. Identify cell pointed.
- Cilia - Elastic cartilage - Small artery - Stratified squamous epi (non - Cardiac muscle - Osteoblast
14. Give its origin. 16. Identify part where point lies. 18. Identify epithelium pointed (specify). keratinizing) 22. Give its distinguishing structural 24. Give its function.
- Kinetosome - Area of fatty degeneration - Endothelium 20. identify tissue below the pointer. characteristic. - Produce bone matrix
- Lymphoid CT - Branching & anastomosing fibers,
presence of intercalated disc
-simple columnar ciliated
25. Identify organ. 27. Identify tissue where pointer lies. 29. Identify structure pointed. 31. Identify tissue pointed. 33. Identify organ. 35. Identify tissue above the pointer.
- Heart - Adipose CT - Purkinje fibers - Pseudostratified columnar ciliated epi - Medium vein - Stratified squamous epi keratinizing
26. Identify part where pointer lies. 28. Give its preponderant fibers. 30. Give its function. 32. Give two (2) examples. 34. Give its thickest layer. type
- Cardiac skeleton - Type 3 & 4 collagen fibers - Impulse conduction - Trachea, bronchi - Tunica adventitia 36. Identify tissue below the pointer.
If tissue: If tissue: - Areolar CT
-cardiac muscle tissue Cardiac muscle tissue
37. Identify structure at tip of pointer. 39. Identify structure pointed. 41. Identify specimen. 43. Identify tissue in field. 45. Identify structure above the pointer. 47. Identify organ.
- Nucleus - Stereocilia - Cerebellum - Mucous glandular epi - Terminal ganglion - Large artery (Aorta)
38. Give its function. 40. Give its function. 42. Identify part where pointer lies. 44. Give one structural characteristic. 46. Identify tissue below the pointer. 48. Identify structure pointed.
- Constructive metabolism, for - Absorption - Granular layer - Reticular & basophilic cytoplasm with M- Smooth muscle - Vas vasorum
reproduction & transmission of heredity flattened nucleus pushed to the basal
part/periphery of the cell
3RD SHORT PRACTICAL
1. Identify structure above the pointer. 5. Identify specimen. 7. Identify tissue left side of pointer. 9. Identify organ. 11. Identify organ (specify).
3. Identify structure pointed.
- Sebaceous glands - Aorta (Organ: large artery) - Serous glandular epi - Lymph node - Thick skin
- Taste buds
2. Identify structure below the pointer. 6. Give its lining epithelium. 8. Identify tissue right side of pointer. 10. Identify structure pointed. 12. Identify structure pointed.
Organ: Tongue
- Arrector pili muscle - Simple squamous epi (Endothelium) - Skeletal muscle - Medullary cords - Sweat glands
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13. Identify specimen. 15. Identify tissue (specify). 17. Identify structure above the pointer. 19. Identify specimen. 21. Identify tissue above the pointer. 23. Identify organ.
- Palatine tonsil - Long bone tissue - Terminal ganglion - Cerebellum - Lymphoid CT - Thymus
14. Identify structure pointed. 16. Identify part where pointer lies. 18. Identify tissue below the pointer. 20. Identify part where pointer lies. 22. Identify tissue below the pointer. 24. Identify structure pointed.
- Salivary corpuscle - Interstitial/Haversian lamellae - Smooth muscle - White matter - Mucous glandular epi - Septa
CELL
MEMBRANE-BOUND ORGANELLES
L/M E/M FUNCTION
Plasma membrane X Trilaminar Barrier
(Semi-permeable possess device for attachment
membrane) regulate passage of substances in & out of
cell
Mitochondria Slender rods/filaments - Acid 2 membranes (trilaminar) Powerhouse of the cell; ATP production
fuchsin/supravital Janus Green
Rough endoplasmic Basophilic clumps - Cresyl Tubules; Cisternae For protein synthesis
reticulum (rER) violet stain
Smooth endoplasmic X Like rER but without cisternae Synthesis of lipids & steroids
reticulum (sER) Detoxification of drugs/substances
Storage & release of calcium
Golgi complex Unstained area near nucleus Saucers; Cisternae Modifies, concentrates & packages secretions
Blackened w/ silver stain Important role for cell membrane renewal
Synthesis of CHO
Lysosomes X Small membrane-bound Digest non-usable intracellular materials
dense bodies
Peroxisomes X Membrane bound bodies Breakdown free radicals
Detoxification
Segregation of H2O2
Annulate lamellae X Cisternae w/ pores *function still obscure*
NON-MEMBRANE BOUND ORGANELLES
Centrosome Pale area; pair of short rods 2 hollow cylinders Organizing center for mitotic spindle formation
(Cell center) perpendicular to each other Give rise to basal bodies (kinetosome), where
cilia & flagella originate
Cytoskeleton X Lattice; structural proteins Contractility of cytoplasm
Protoplasmic streaming
Viscoelasticity of cytoplasm
Proteasomes X Protein complexes Degrade individual denatured/non-functional
proteins into short polypeptides
NUCLEUS PARTS
Nucleus Controls constructive metabolism of the cell
Essential for reproduction & transmission of
heredity
Nuclear envelope Thin dark line around nucleus 2 layers with perinuclear Enclose/separate nuclear content from
space in between cytoplasm
Nucleolus Round small body; eccentric Tightly coiled RNA filaments Site for ribosome production/formation
Chromatin Deeply stained clumps DNA strands
throughout nucleoplasm
EPITHELIUM
TYPES PICTURES ORGANS FUNCTION
1. SIMPLE SQUAMOUS • Parietal layer of Bowman’s capsule Filtration & diffusion
EPITHELIUM • Thin segment of Henle’s loop
- Single layer of thin plate-like cells • Rete testis
- TOP VIEW: irregular hexagons, • Lung alveoli
serrated interlocking borders
- Perpendicular section: flattened,
attenuated, spindle shape
CONNECTIVE TISSUE
TYPES PC PF OCC FUNCTION
MESENCHYMAL CT ▪ Between germ layers ▪ Embryo packing
▪ developing organs of ▪ material
Mesenchymal cells Collagen embryo
ELASTIC FIBERS
MUSCLE TISSUE
SMO - Structural & Functional unit: Muscle Fiber Give 1 characteristic of muscle
OTH - Shape of Cell: Spindle-Shaped tissue.
MUS - Manner of contraction: Slow, Sustained, - Contractility
CLE Resistant to fatigue - Excitability
- Unstriated, Involuntary, 1 nucleus - Undergo - Extensibility
peristalsis - Elasticity
- L-Section: Fusiform Densities, Thick Middle, Give 2 functions of muscle
Thinly Tapered, Elongated, Uninucleated, Arranged tissue.
Offset, Muscle fibers are surrounded by - responsible for body’s
elastic/reticular fibers movement
- X- Section: Only larger section have round central - maintenance of body posture
nucleus, Variable sizes due to tapered ends, Mosaic - stabilize joints
of Irregular Polygons - heat generation
- Function: (Gap/Nexus Junction)- Cell to cell
attachment, Rapid spread of excitation
- Occ.: Reproductive organs,
GI/Genito-Urinary/Respiratory tract, Walls of BVs,
Iris of the eye
NERVOUS TISSUE
NERVE Fascicle
(X-section) Endoneurium, Perineurium,
Epineurium
Vas nervorum
Neurilemma
Schwann cell
Myelin Sheath
SMALL Perineurium
NERVE Schwann cell
CEREBELLU Molecular layer
M Purkinje cell layer
Granular layer
White matter
SENSORY Nucleolus
GANGLIA Nucleus
(X-section) Lipofuscin
Satellite cell
Ganglion
Flattened cell
CARDIOVASCULAR SYSTEM
HEART What is its supporting structure? - Cardiac Label:
skeleton 1. Myocardium
Give its functions. 2. Endocardium
- generating blood pressure 3. Cardiac skeleton
- routing of blood 4. Purkinje fibers
- ensuring one-way blood flow
- regulation of blood supply
Identify the structure where pointer lies: (1)
subendocardial layer &(2) Purkinje fiber
AORTA Label:
(large 1. Tunica intima
artery) 2. Tunica media
3. Tunica adventitia
4. Vas vasorum
LYMPHATIC SYSTEM
LYMPH Label: ▪ Axilla Stroma: - capsule, trabeculae,
NODE 1. Capsule ▪ Inguinal area (groin) reticular fiber networks & cells
2. Subcapsular ▪ Cervical (neck) Parenchyma: - Cortex (Corona
sinus ▪ Mesentery & GCF), medulla (medullary
3. Medullary cords)
chords Lymphatic sinuses:
4. Medullary sinus - mesoderm Subcapsular, Cortical,
Medullary
- Filtration of lymph,
Production of lymphocytes,
Immune defense
DIGESTIVE/GASTROINTESTINALTRACT
ESOPHAGUS GASTRO-ESOPHAGEAL JUNCTION FUNDUS OF STOMACH PYLORUS OF STOMACH
1. Stratified squamous 1. Esophageal end 1. Gastric pit 1. Gastric pit
epithelium 2. Cardia of stomach 2. Inter-foveolar area 2. Pyloric gland
2. Muscularis mucosae 3. Cardiac glands 3. Parietal cell 3. Muscularis mucosae
3. Cystically dilated duct 4. Muscularis mucosae 4. Zymogenic cell 4. Tunica submucosa
4. Tunica muscularis
DUODENAL ILEUM APPENDIX COLON