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1. Cell Structure,
Reproduction and
Development
Eyepiece graticule: fitted into the eyepiece of the
microscope
and is used to measure objects.
1.1. Observing Cells Has no units and is calibrated by the stage
micrometre which has
an accurate scale (in mm) and
Cells are the basic units of all living organisms. provides reference dimensions.
Organisms can be unicellular or multicellular. 1mm= 1000 μm
New cells form by the division of existing cells. 1μm= 1000 nm
Cells are too small to be seen by the naked eye, therefore Use the same magnification when calibrating the
microscopes are used to magnify eyepiece
graticule and when using it to measure the
Light Microscopes: specimen.
Uses light to shine through the specimen, then
refracts through
glass lenses
An image is projected onto the eyepiece
Cheap, therefore commonly available in schools
Quick observation of cells
Preservation and staining of slides could cause
artefacts
Much lower magnification and resolution compared
to electron
microscopes
Electron Microscopes:
Uses beams of electrons to create an image
Electromagnetic lenses form an image
An image is projected onto a fluorescent screen Calculation example:
Due to the electron’s low wavelength compared to
visible light, high
magnification and resolution can be
achieved
Specimens are required to be in a vacuum, to prevent
disturbance of
electrons
Water boils in a vacuum, so specimen should be dead
Extremely expensive to buy and maintain
Specimens must undergo lengthy preparation before
use
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New DNA checked, and errors are repaired. Hard to find mates
Centrioles replicate. Resources wasted by forming gametes
Food storage is increased.
In meiosis, chromosomes are halved from diploid (2n)
Mitosis: nuclear division producing two genetically cells to
haploid (n) cells
identical
daughter nuclei, each containing the same no. of Thus, chromosome number is kept constant instead of
chromosomes as the
parent. doubling every
generation
Stages of Mitosis: Stages of Meiosis:
secondary
spermatocytes which continue with
1.5. Sexual Reproduction and Meiosis meiosis II forming 4
spermatids that mature into
spermatozoans
Features of Sexual Reproduction:
Formation of gametes
Uses meiosis cell division
Leads to genetic variation
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Oogenesis (Females):
Takes place in the ovaries Sperm Gamete:
Germinal epithelial cells divide by mitosis to produce
diploid
oogonia
Oogonia start meiosis and become primary oocytes
(still diploid),
but meiosis stops at prophase I
All this occurs before a baby girl is born and at birth
has around
400 000 primary oocytes Acrosome: A membrane-bound storage site for
At puberty, primary oocyte continues to finish meiosis enzymes that digest
the layers surrounding the ovum
I to produce
secondary oocyte and first polar body to penetrate it.
(small haploid cell with less
cytoplasm; degenerates) Nucleus: Contains highly condensed genetic material,
Each month one secondary oocyte is released into the therefore
less energy is required to carry it
oviduct to get
fertilised Neck/Middle: Tightly packed mitocondion is present.
If fertilisation occurs, secondary oocyte undergoes They provide
the energy for lashing the tail to move
meiosis
II to form an ovum and second polar body Tail: A complex flagellum (9+2 microtubule) that
If ovum is fertilised, then a diploid cell is formed: creates a
whipping motion to propel it forwards
zygote embryo
fetus
Egg Gamete:
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gamete
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Double fertilization occurs, in which 1 nucleus fuses with It then undergoes several process, including intron
the
Egg cell, forming a diploid zygote. The other nucleus removal, by
splicosomes to produce the mRNA.
fuses with the
2 polar nuclei forming a triploid By this process, multiple different proteins could be
endosperm nucleus. produced from
1 gene.
After fertilization, the ovule becomes a seed, The zygote Epigenetics: The study of the factors that affect genes
becomes an
embryo, and the endosperm becomes food other
than the base sequence of the DNA.
storage. DNA Methylation: It’s when a methyl group is added,
surpressing
a gene.
1.10. Cell Differentiation DNA Demethylation: The removal of the methyl group,
causing a
gene to be reactivated.
Cell differentiation allows the rise of specialized cells. Histone acetylation: Addition of acetyl group, causing
Certain genes are surpressed, while others remain active. transcription of a certain gene.
Locus: position at which a particular gene is found on a Histone methylation: This is different to DNA methylation.
particular chromosome. It
depends on the position of the lysine (where methyl is
Polygenic Traits: Involve multiple genes and usually added).
affected by
the environment e.g. height. It gives rise to
continuous variate
i.e. a phenotype measurable on a 1.12. Stem Cells
scale.
Digenetic inheritance: Deals with the inheritance of two Stem cells: cells that divide repeatedly by mitosis, and
separate genes on different chromosomes at once differentiate into specialized cells or remain as stem cells.
They usually invole ratios to be known (9:3:3:1) There are three different kinds:
During experiments, ratios may be unexpected due to Totipotent: cells that can divide repeatedly to form
a small
sample size, experimental errors. any other
cell in the body, e.g. zygote
Pluripotent: embryotic stem cells that lead to
development of
the embryo and later the adult. They
can not specialized into
placenta.
Multipotent: Adult stem cells that are only able to
produce a
few types of cells e.g. stem cells in bone
marrow.
Blastocyte: An early embryo made of a hollow ball of cells
with
pluripotent cells on the inside, forming an organism
Morula: An early staged embryo of 16 cells
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Plant cells are connected to each other by the middle Chloroplast: It is responsible for producing food in a plant
lamella, which
is made of pectin. cell, it contains many parts:
The cell wall is made up of cellulose fibres and matrix. Thylakoid
The matrix is made of hemicellulose and pectin. It helps Granum
hold the
cellulose fibres together. Stroma
Cellulose microfibres contain around 60-70 microfibriles. Starch Grain
For the transfer of materials between cells, regions called Ribosomes
pits
contain plasmodesmata. This region has no cell wall
allowing
exchange of substances between cells.
In dead cells, like the xylem, a secondary cell wall is
present that
covers the plasmodesmata. It provides
support and rigidity.
Secondary cell wall contains cellulose at angles and lignin.
It is
waterproof which causes the cell to die.
Celluose:
It is a polysaccharide made up of β-glucose molecules
Hydrogen bonds formed between molecules
They form long straight chains
Every other molecules flips, allowing glycosidic bonds
to form Amyloplast: Responsible for startch storage
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N (N −1)
Diversity Index: D = ∑ n(n−1)
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Biology