Performance Task – Lab Activity

CELLULAR RESPIRATION IN YEAST

Name: _________________________________________________________________________________________ Score: ________________
Grade Level and Section: ____________________________________________________________________ Date: _________________

Objectives
1. _________________________________________________________________________________________________________________________________
2. _________________________________________________________________________________________________________________________________
3. _________________________________________________________________________________________________________________________________

Hypotheses
1. _________________________________________________________________________________________________________________________________
2. _________________________________________________________________________________________________________________________________
3. _________________________________________________________________________________________________________________________________

Materials:
- 4 pcs of 250mL / 500mL plastic bottle
- 6 pcs balloon
- Active dry yeast
- Warm water
- Masking tape, manila paper, tissue paper
- White table sugar / sucrose (1/4 kg)
- Ruler / yarn

Procedure:
1. Prepare 1%, 5%, and 10% sucrose solution:
a. 1%: add 0.5 g of sugar to 49.5 mL of water
b. 5%: add 2.5 g of sugar to 47.5 mL of water
c. 10%: add 5 g of sugar to 45 mL of water
2. Add 23 mL of the appropriate sucrose solution to each bottle container. Label the bottles.
3. Add 2 mL of yeast to each bottle container (measured in a 10mL graduated cylinder).
4. Add warm tap water and 2mL of yeast to another bottle container. This will serve as your control.
5. Place a balloon on the top of each tube and tape around the base of the balloon to assure it’s secure. Make sure
that there is NO AIR in the balloon before placing it on top of the bottle. Stretch the balloon a few times before
using it.
6. With your palm sealing the top, shake each tube until the yeast is dissolved.
7. Measure the depth of bubbles produced.
8. Observe how the balloons change after 10 minutes, 20 minutes, 30 minutes, and 1 hour. Measure the balloon at
its widest point with string, then measure the length of the string and record the circumference (in cm) in the
data table.

Data Collection:

Depth of Carbon Dioxide Bubbles (cm)

Sucrose Treatment 10 minutes 20 minutes 30 minutes 1 hour
Tap water
1% sucrose
5% sucrose
10% sucrose

Balloon Circumference Measurement (cm)

Sucrose Treatment 10 minutes 20 minutes 30 minutes 1 hour
Tap water
1% sucrose
5% sucrose
10% sucrose
Graph (2 separate graphs for the two tables)

Analysis Questions:

1. Write the equation for cellular respiration.

2. What is the purpose of cellular respiration?
3. What are the two types of fermentation and what is produced in each?
4. What type of fermentation do yeast perform?
5. What gas that is produced by the yeast? How is this formed?
6. What process is shown by the experiment? Explain this process in detail using the experimental setup.
7. Why must all the bottle containers be set up in exactly the same way except for the differences in the sucrose
concentration?
8. How do we know which sucrose concentration provides more energy for the yeast? 6
9. What is the yeast breaking down in the process? What is the yeast making in this process?

Conclusions (based on hypotheses):

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 Appendix A
Photos with Label

Appendix B
Table of Contributions

Student’s Name Contribution / Work Done Remarks