Bio 190 - Recombinant Dna Technology - 10-22-2019

MHAM COLLEGE, INC. | R.
DUTERTE STREET, BANAWA, CEBU CITY, PHILIPPINES

COLLEGE OF ARTS AND SCIENCES | BACHELOR OF SCIENCE IN BIOLOGY
OUTCOMES-BASED EDUCATION (OBE) CURRICULUM
TOTAL NUMBER OF
SUBJECT CODE: BIO 190 COURSE TITLE: RECOMBINANT DNA TECHNOLOGY 17
PAGES:
REVISION PREREQUISITE/
0 / 10-22-2019 CREDIT UNITS / CONTACT HOURS:
NO./DATE S: CHEM 45 (Biochemistry)
SUBJECT PLACEMENT: ZOO 101 (General Zoology) Lecture: 2 u 2 hours per week
Fourth Year, First Semester Laboratory: 1 unit / 3 hours per week
COURSE DESCRIPTION:
This course deals with the theoretical and applied aspects of basic biotechnology techniques for the study of DNA and proteins. In the laboratory students will apply theory and practical
skills from this and previous courses to perform standard molecular biology techniques for the isolation, manipulation and analysis of DNA as well as the expression and purification of
protein.
COURSE OUTCOMES (CO):

CO1 Demonstrate practical experience of selected Molecular Biological Techniques.
CO2 Demonstrate the basic techniques involved in recombinant DNA manipulations including DNA restriction, ligation, transformation and selection of recombinant plasmid.
CO3 Demonstrate the principle of PCR and its applications (e.g., Analysis of DNA repeats to estimate its frequency in the population).
GRADING SYSTEM
The grade for the entire course will be comprised of the following: Cumulative Summation
The grade per grading period will be comprised of the following: Lecture = 60%, Laboratory = 40%
The breakdown of the grades for the lecture and laboratory is as follows: Grade Equivalent Grade Equivalent
1.00 96 – 100 R Repeat
Lecture (Cumulative Summation) Laboratory (Cumulative Summation) 1.50 91 – 95 Failure
Major Examination Major Examination 2.00 86 – 90 0.00 Cheating
Quizzes Quizzes 2.50 81 – 85 Excessive Absences
Performance Laboratory Exercise
Other Sources Proficiency / Practical Examination 3.00 75 – 76 DR Dropped
Reflective Journal Laboratory Manual 3.50 42 – 48 INC Incomplete
4.00 7 – 13
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MHAM COLLEGE, INC. | R. DUTERTE STREET, BANAWA, CEBU CITY, PHILIPPINES
INSTITUTIONAL – MHAM COLLEGE INC.

VISION:
A leading institution of higher learning producing universally competitive, morally upright and service-oriented professionals.
MISSION:
The MHAM College is committed to create a proactive and excellent learning and teaching environment through inculcation of its core values.
CORE VALUES
The institution is anchored on these core values to achieve the College Mission:
1. Professionalism. We observe a polite behavior and courteousness; adhere to a high sense of ethical standards in the performance of our duties and responsibilities in the work place.
2. Respect. We act with deep admiration, give due regards and high esteem to everyone.
3. Oneness. We stand united in all our endeavors and uphold the good name, honor and integrity of the institution.
4. Leadership. We act as role model, do things that will work best in the institution and provide policy directions that are relevant to the needs of the time in order to fulfill our
ultimate goals.
5. Integrity. We adhere to the highest ethical code of conduct in our dealings with others and exercise our duties and responsibilities with correlative obligation to preserve human
dignity.
6. Fairness. We are impartial, reasonable, transparent and free from discrimination in the performance of our duties and responsibilities.
7. Excellence. We pursue the highest standard of quality service in all programs of engagements and undertakings for the attainment of the vision and mission of the institution.
INSTITUTIONAL GOALS:
1. To provide quality instruction, research, community services and production of technologies.
2. To develop and maintain programs for enhancing and sustaining college functions and student success.
3. To provide quality and state-of-the-art infrastructures.
4. To promote respect for life and environmental care.
5. To provide academic leadership in fields of specialization that the institution pursues.
6. To establish linkages and partnerships with various institutions.
COLLEGE OF ARTS AND SCIENCES

GOALS AND OBJECTIVES:
1. To train each student with the required skills for national development and initiate and foster the appropriate and relevant knowledge, attitudes, skills and values to enable him/her to
become useful, productive and gainfully employed member of society.
2. To develop and maintain the integrity of the profession or disciplines that will provide leadership for the nation.
3. To advance the frontiers of knowledge through research work and apply the technology for improving the quality of human life and responding effectively to changing societal needs
and conditions.
PROGRAM OUTCOMES (PO):
PO 1 Develop an in-depth understanding of the basic principles governing the science of life;
PO 2 Utilize techniques and procedures relevant to biological research work in laboratory or field settings;
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PO 3 Demonstrate effective written and oral communication skills;

PO 4 Engage in community-oriented activities;
PO 5 Apply basic mathematical and statistical computations and use appropriate techniques/procedures relevant to biological research work in laboratory or field settings;
PO 6 Extend knowledge and critically assess current views and theories in various areas of biological sciences;
PO 7 Demonstrate interpersonal skills, leadership qualities, and ethical practice of the profession; and
PO 8 Engage in lifelong activities.
COURSE OUTCOMES (CO):
CO1 Demonstrate practical experience of selected Molecular Biological Techniques.
CO2 Demonstrate the basic techniques involved in recombinant DNA manipulations including DNA restriction, ligation, transformation and selection of recombinant plasmid.
CO3 Demonstrate the principle of PCR and its applications (e.g., Analysis of DNA repeats to estimate its frequency in the population).
RELATIONSHIP OF COURSE OUTCOMES (CO) TO PROGRAM OUTCOMES (PO) – CURRICULUM MAPPING
PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO 10
CO1
CO2
CO3
Legend:
L – Facilitates learning of competency
P – Allows student to practice competency (no input, but competency is evaluated)
O – Opportunity for development (no input or evaluation, but competency is practiced)
COURSE CONTENT (LEARNING PLAN)

WEEK LEARNING OUTCOMES TOPICS METHODOLOGY RESOURCES ASSESSMEN TOOL
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Classroom Laboratory
T
Instruction Instruction
At the end of the lecture, the Orientation on the general rules, Class orientation Pre-lab/ Post-lab Text Book / Written Answer
learner will be able to: requirements, and grading Discussion Reference Books Exam / key
system of the course Lecture Quizzes
1. Define terms in relation to
the fundamental principles PowerPoint Performance of PowerPoint Slides
PART 1: MOLECULAR Experiment Oral Rubrics
and theories of molecular Presentation
BIOLOGY Internet Information Recitation
biology. Small group
Lecture: Rubrics
2. Describe the structure and discussion Journal Experimental
organization of genes, I. Fundamentals of Molecular
Biology Reflection logs Results
1
chromosomes and the Laboratory / Rubrics
I.1. History of Molecular
human genome. Case studies Working manual Post lab
Biology
3. Compare and contrast I.2. Review on cell function Reports Answer
different types of and structure key
polymorphisms. I.3. Principles of genetics and Moving /
4. Discuss mitochondrial its applications to disease Practical
DNA typing. transmission Exam
I.4. Structure, function, and
duplication of RNA and
DNA
I.5. Polymorphisms
At the end of the lecture, the Lecture: Lecture Pre-lab/ Post-lab Text Book / Written Answer
learner will be able to: II. Mutations and Genetic Discussion Reference Books Exam / key
PowerPoint
1. Define and differentiate Abnormalities Quizzes
Presentation Performance of PowerPoint Slides
the different types of II.1. Definition of terms
genetic mutations. Small group Experiment Oral Rubrics
II.2. Gene Mutations: Internet Information
2. Define various mutations a. History discussion Recitation
and genetic abnormalities b. Types Rubrics
Reflection logs Journal
2 which can result in genetic  Hereditary Experimental
diseases.  Somatic Case studies Results
Laboratory / Rubrics
3. Explain the effects of  De Novo Working manual
c. Causes Post lab
human gene abnormalities Reports Answer
and its effect on health and  Spontaneous
 Error-prone key
development. Moving /
replication
bypass Practical
Exam
Page 4 of 15
METHODOLOGY
ASSESSMEN
WEEK LEARNING OUTCOMES TOPICS Classroom Laboratory RESOURCES TOOL
T
 Induced
 Errors introduced
during DNA
repair
d. Classifications by
effect:
 Structure
 Function
 Fitness
 Impact on protein
sequence
 Inheritance
 Special classes
3–4
learner will be able to: III. Chromosomal Structure Discussion Reference Books Exam / key
1. Describe the chromosomal and Mutations PowerPoint Quizzes
structure and its functions. III.1.Definition of terms Presentation Performance of PowerPoint Slides
2. Define and differentiate III.2.Structure and Function Small group Experiment Internet Information Oral Rubrics
the different types of a. Bacterial chromosome discussion Recitation
genetic mutations. b. Prokaryotic Journal Rubrics
chromosome Reflection logs Experimental
3. Define various
c. Eukaryotic Laboratory / Results
chromosomal Case studies Working manual
chromosome Rubrics
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abnormalities which can d. Human chromosome Post lab

result in genetic diseases. III.3.Types of chromosomes: Reports Answer
4. Explain the effects of a. Metacentric key
human gene abnormalities b. Submetacentric Moving /
c. Acrocentric Practical
and its effect on health and
d. Telocentric Exam
development.
III.4.Genetic Defects:
a. Chromosome Defects:
 Aneuploidy
 Deletion
 Inversion
 Ring
METHODOLOGY
ASSESSMEN
T
 Translocation
 Mosaicism
b. Single-gene Defects
 Dominant
3–4  Recessive
 X-linked
III.5.Inheritance of genetic
abnormalities / defects
III.6.Environmental influence
III.7.Genetic testing
5
At the end of the lecture, the PART 2: MOLECULAR Lecture Pre-lab/ Post-lab Text Book / Written Answer
learner will be able to: DIAGNOSTICS Discussion Reference Books Exam / key
1. Gain a solid foundation in the PowerPoint Quizzes
Lecture: Presentation
most commonly utilized Performance of PowerPoint Slides
IV. Analysis and
molecular diagnostic testing Small group Experiment Oral Rubrics
Characterization of Nucleic
protocols. discussion Internet Information Recitation
Acids and Proteins
2. Apply the knowledge of Rubrics
IV.1. History and Applications Reflection logs
molecular testing to the most Journal Experimental
IV.2. Methods:
commonly performed Case studies Results
 Purification
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applications in the clinical  Determining purity Laboratory / Rubrics

laboratory such as: nucleic by Working manual Post lab
acid extraction, resolution spectrophotometry Reports Answer
and detection, analysis and and fluorometry key
characterization of nucleic  Quantification – real- Moving /
acids and proteins, nucleic time PCR, Practical
acid amplification and DNA spectroscopic nucleic Exam
sequencing. acid quantitation,
3. Apply knowledge of quality DNA microarray,
assurance in the performance SAGE, etc.
of molecular-based  Synthesis –
diagnostic methods. polymerase chain
reaction
 Kinetics
 Gene expression –
northern blot,
southern blot, etc.
METHODOLOGY
ASSESSMEN
T
Answer
Periodical /
6 PRELIMINARY EXAMINATION Written /Practical Examinations Test Questionnaires key /
Practical exam
Rubrics
7
learner will be able to: V. Nucleic Acid Extraction Discussion Reference Books Exam / key
and Detection PowerPoint Quizzes
1. Gain a solid foundation in the
V.1. Definition of terms Presentation Performance of PowerPoint Slides
most commonly utilized
molecular diagnostic testing V.2. Purpose of nucleic acid Small group Experiment Oral Rubrics
protocols. extraction Internet Information Recitation
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2. Apply the knowledge of V.3. Isolation Methods: discussion Rubrics

molecular testing to the most a. Organic isolation Journal Experimental
commonly performed b. Inorganic isolation Reflection logs Results
applications in the clinical c. Solid-phase isolation Case studies Laboratory / Rubrics
laboratory such as: nucleic d. Crude lysis Working manual Post lab
acid extraction, resolution e. Affinity purification Reports Answer
and detection, analysis and V.4. Isolation of mitochondrial key
characterization of nucleic DNA Moving /
acids and proteins, nucleic V.5. Isolation of RNA Practical
acid amplification and DNA V.6. Ribonuclease Exam
sequencing. a. Function
3. Apply knowledge of quality b. Classification
assurance in the performance c. Specificity
of molecular-based d. Contamination in
diagnostic methods. RNA extraction
8
At the end of the lecture, the Lecture (Molecular Diagnostic Lecture Pre-lab/ Post-lab Text Book / Written Answer
learner will be able to: Methods – continued): Discussion Reference Books Exam / key
PowerPoint Quizzes
1. Gain a solid foundation in the VI. Genetic Polymorphism Presentation Performance of PowerPoint Slides
most commonly utilized a. Metabolic phenotyping
molecular diagnostic testing Experiment Oral Rubrics
b. Genotyping methods: Small group Internet Information
protocols. Recitation
 PCR with RFLP discussion Rubrics
2. Explain the principles of the Journal Experimental
analysis Reflection logs
molecular diagnostic  Multiplex PCR Results
methods. Laboratory / Rubrics
 Allele-specific Case studies Working manual
3. Identify the advantages and
amplification Post lab
disadvantages of using Reports Answer
 Fluorescence key
molecular-based methods. methods Moving /
Practical
Exam
Page 8 of 15
METHODOLOGY
ASSESSMEN
T
At the end of the lecture, the Lecture (Molecular Diagnostic Lecture Pre-lab/ Post-lab Text Book / Written Exam Answer
learner will be able to: Methods – continued): Discussion Reference Books key
1. Identify the advantages and PowerPoint Oral
VII. Microorganisms Presentation PowerPoint Slides Recitation
disadvantages of using Performance of
a. Mycobacterium Rubrics
molecular-based methods as Small group Experiment Internet Experimental
b. Histoplasma
compared to traditional discussion Results Rubrics
c. Coccidioides
culture-based methods. Journal
d. HIV Reflection logs
9 2. Explain the value of controls, Post lab
e. HCV Laboratory /
in particular amplification Case studies Reports Rubrics
f. Streptococcus Working manual
controls, in ensuring the
pyogenes Moving / Answer
reliability of PCR results.
g. Neisseria gonorrheae Practical key
3. Compare and contrast the
h. Chlamydia trachomatis Exam
molecular methods that are
i. Others
used to type bacterial strains
in epidemiological
investigations.
10
1. Explain the principles of the PowerPoint Oral
VIII. Inherited Diseases Presentation PowerPoint Slides Recitation
molecular diagnostic Performance of
a. Factor V Leiden Rubrics
methods. Small group Experiment Internet Experimental
Mutation
2. Identify the advantages and discussion Results Rubrics
b. Sickle Cell Anemia
disadvantages of using Journal
Page 9 of 15
molecular-based methods of c. Cystic Fibrosis Reflection logs Post lab

detecting inherited genetic d. Fragile X Syndrome Laboratory / Reports
diseases. e. Huntington’s Disease Case studies Working manual Rubrics
f. Prader-Willi Syndrome Moving /
Practical Answer
g. Others key
Exam
11
PowerPoint Oral
1. Explain the principles of IX. Neoplastic Diseases Presentation PowerPoint Slides
Performance of Recitation
the molecular diagnostic Rubrics
methods. Small group Experiment Internet Experimental
1. Targets:
2. Identify the advantages a. Tissue-specific markers discussion Journal Results Rubrics
and disadvantages of using (antigens, gene Reflection logs Laboratory /
rearrangements) Post lab
molecular-based methods Working manual Reports Rubrics
of detecting inherited Case studies
genetic diseases. Moving / Answer
Practical key
Exam
Page 10 of 15
METHODOLOGY
ASSESSMEN
T
3. Contrast tissue-specific b. Disease-specific

and tumor-specific markers (translocations,
molecular targets. point mutations,
polymorphisms in
4. List molecular targets that
tumor suppressor or
are useful for diagnosis oncogenes)
and the monitoring of solid c. Viruses (EBV, HCV,
tumors. HTLV-1)
5. Describe translocations
associated with 2. Methods:
hematological a. Hybridization, blotting
11 b. Standard PCR, RT-
malignancies that can be
PCR, electrophoresis
used for molecular testing. c. PCR with
heteroduplex analysis,
SSCP
d. Real-time PCR with
gene or patient-specific
probes
e. Translocations Used in
Diagnosis and
Monitoring of
Hematological Tumors
Answer
Periodical /
12 MIDTERM EXAMINATION Written /Practical Examinations Test Questionnaires key /
Practical exam
Rubrics
13 – 14
PowerPoint Oral
1. Explain the principles of X. DNA-based Tissue Typing Presentation Performance of PowerPoint Slides Recitation
the DNA-based molecular X.1. Introduction to HLA
Experiment Rubrics
typing methods. antigens and Major Small group Internet Experimental
2. Describe the laboratory Histocompatibility discussion Results Rubrics
Journal
methods used to identify Complex (MHC)
Page 11 of 15
HLA antigens by serology X.2. Methods: Reflection logs Post lab

a. Sequence-specific Laboratory / Reports Rubrics
testing. Case studies
oligonucleotide probe Working manual
4. Identify the advantages Moving / Answer
and disadvantages of using hybridization key
b. Reverse SSO Practical
molecular-based methods Exam
hybridization
as compared to traditional c. Sequence-specific PCR
serology-based methods. d. Sequence-based Typing
3. Describe the different
tissue typing discrepancies
METHODOLOGY
ASSESSMEN
T
15 – 17
At the end of the lecture, the Lecture: Lecture Pre-lab/ Post-lab Text Book / Written Exam Answer
learner will be able to: XI. Quality Assurance in the Discussion Reference Books key
Molecular Biology PowerPoint Oral
1. Describe the elements that Presentation PowerPoint Slides
Laboratory Performance of Recitation
must be addressed in the Rubrics
quality assurance plan XI.1. Overview Small group Experiment Internet Experimental
a. Sample collection discussion
during the pre-analytical, Results Rubrics
b. Transport Journal
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analytical, and post- c. Processing Reflection logs Post lab

XI.2. Contamination Control Laboratory / Reports
analytical phases of Case studies
XI.3. Positive and Negative Working manual Rubrics
molecular testing. Moving /
2. Explain the importance of Controls Answer
XI.4. Considerations for Practical
quality assurance and Exam key
Laboratory Personnel
quality control in the XI.5. Assay Validation
molecular diagnostic a. Pre-analytical
laboratory. Phase
b. Analytical Phase
c. Post-analytical
Phase
Periodical
18 FINAL EXAMINATION Written / Practical Examinations Test Questionnaires
exam
REFERENCES:
o Alberts, Bruce, Watson, J., MOLECULAR BIOLOGY OF THE CELL. Garland Science, 1983
o Buckingham, Lela, MOLECULAR DIAGNOSTICS: FUNDAMENTALS, METHODS, AND CLINICAL APPLICATIONS. 2007
o Coleman, William B., Tsongalis, Gregory J. MOLECULAR DIAGNOSTICS: FOR THE CLINICAL LABORATORIAN. Springer Science+Business Media,
1997
o Craig, Nancy Lynn,MOLECULAR BIOLOGY: PRINCIPLES OF GENOME FUNCTION. 2010
o Craig, Nancy, MOLECULAR BIOLOGY: PRINCIPLES OF GENOME FUNCTION. Oxford University Press, USA, 2010
o Elliott, William H., BIOCHEMISTRY AND MOLECULAR BIOLOGY. 1997
o Lee, Martin E., Saunders, Nick A., REAL-TIME PCR: ADVANCED TECHNOLOGIES AND APPLICATIONS. 2013
o Lodish, Harvey, Baltimore, D., MOLECULAR CELL BIOLOGY. 1986
o Patrinos, George P., MOLECULAR DIAGNOSTICS, 3rd Ed. Academic Press, 2017
Page 13 of 15
CLASS POLICIES:
1. Short quizzes may be given before or after lecture-discussion during class schedule.
2. Long/Unit quizzes/exams usually cover previously discussed topics and shall be given before the start of the lecture during the next meeting.
3. Punctuality is of utmost importance. A student who comes in late in class and misses the quiz or some parts of it will not be given consideration. However, valid reasons like accidents,
hospitalization or death of immediate family may be given into consideration with pertinent papers to be presented (police or CITOM report, medical and death certificates).
4. No special quizzes, in general, shall be given. In the event that a student misses a quiz due to a reasonable absence, he/she must immediately inform the instructor when he/she comes in and
present a valid proof as to why he/she was absent. Only then can giving a special quiz may be considered. No special quiz, however, will be granted to any student who misses a laboratory
quiz/exam that requires much preparation and set-up.
5. In cases where a student misses a major examination, he/she may be given a special examination provided that he/she can show a valid proof that clearly shows the reason for missing the
examination. The proof must be presented within one week after the student returns. Otherwise, no special examination shall be granted. Fill up a form for special quiz or term exam from
the office of the dean.
6. Assignments/seat works/library works/laboratory exercises should be passed on time. Late assignments shall be given a deduction (at least 75% will be the highest possible score).
7. Students must follow the seat plan. A student may be marked as absent if not in his/her designated seat.
8. Strictly no communication, in any form, during quizzes and major examinations. (Please refer to the student handbook). Such communications may be treated as a case of cheating and the
student/s shall receive a grade of zero (0) in that exam for the first offense and a grade of 0.00 in the subject for succeeding violations.
9. Students have the right to ask about their grades. The instructor will show the class record every end of the grading period. All clarifications / corrections / questions / complaints must be
addressed to the instructor.
Page 14 of 15
NOTED BY: APPROVED BY:

PREPARED BY:
___________________________________________
EMMANUEL A. PARDIÑAN, M Sc, DOD
__________________________________________ ______________________________
Department Chairman, BS-Biology
JOSE M. OCLARIT, MS-Bio, PhD ARLENE M. DIAZ, MD, EdD
College of Arts and Sciences
College Dean, College of Arts and Science Vice-President for Academics
Page 15 of 15

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MHAM COLLEGE, INC. | R.

DUTERTE STREET, BANAWA, CEBU CITY, PHILIPPINES

COURSE OUTCOMES (CO):

INSTITUTIONAL – MHAM COLLEGE INC.

COLLEGE OF ARTS AND SCIENCES

PO 3 Demonstrate effective written and oral communication skills;

RELATIONSHIP OF COURSE OUTCOMES (CO) TO PROGRAM OUTCOMES (PO) – CURRICULUM MAPPING

COURSE CONTENT (LEARNING PLAN)

abnormalities which can d. Human chromosome Post lab

applications in the clinical  Determining purity Laboratory / Rubrics

2. Apply the knowledge of V.3. Isolation Methods: discussion Rubrics

molecular-based methods of c. Cystic Fibrosis Reflection logs Post lab

3. Contrast tissue-specific b. Disease-specific

HLA antigens by serology X.2. Methods: Reflection logs Post lab

analytical, and post- c. Processing Reflection logs Post lab

NOTED BY: APPROVED BY:

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