Lnwnent

Am ALmalu0n af andml o/ Lalcum m miu

'

Maloruala Retuwed Teat lulkea lkeake Tomdard

ask, kiketie, eieallosk ele
Chemtea Jdk_ Ma-EDTA MaS0 _plenelpkalin
eqpecl mduntis NaOH elutuen eriochse-me Lloek T
Lncbtalns,EDTA
hepa meple
Lleum deen le cone im alace uhesueue
dgeeita e L maat 'akunelced clemned
lu maj m ezUh ceaut 0 humam eody
B e masl alkumdaid melalue elemed
dhe hunem loooy 11, a uc uemd m Qus
JsoneL_adleéth_lue'e uil nel sute
Cumou a Caleum u mlR y_dd mA a Lmplezoinelue
balonl uullh E DTA Jolt af Lediun lhglene
dicmmmetelaceate)

PRCedure
nepaLation Ma EDTA ndLcalor
Take 50ml a M-EDTA Jolulion ima 250m Jseakeesz
anel
The a-E DT'A Jsllnn enlans O.7hq al EDTA
O41 MqSO,i nlI0Om aualer
dalel 23 duaps q phenclplthalein indicalez_onel
O.M NaOH Jhlllan derakuwe Ta ihe keake
wtilit keecanes biusk,lount henuher af die=
and du.ccee heddluten
 2

3 Take 50ml aMa-EDTA Aauuen 190m,

in a S0m
Jenkee adel adel clatpl af.IM NaOH Aclution
aeleed n (2 amd make 'tatal Linl welune 95nl
adeled
Linq utlleeLte
h chelsl2ml ph 10 kutler elihe_and Bduaps af
esucIune nk Tilcatan e the alsaue 'Lelior-
hAtAsk dheore_asue 2kaskilites
e Lald u ed in Laleuiacldl 0.01HEDTA
aulue daakuuue utiluaulen dun elue
auilon umA dedue, adel 00IM Mq SC seluusn
diapse usbdalubor tie S¬cand thtsn add
D.orMEDTA JsuluendsokutLe uill Jelolucean

Ewnng al calcuun
1 ikAte cull S0m e af milk Lalilitn ule a ?5
CoMEalask
Adel mapH 10 ullo1Oa M-EDTALnlicatan
Jsldinnohe 3 dadki al eiackoome Llask Iilirates
3 Tkate it ul tandaculooIM EDTA
Jation utilthe

4 Keptat euIaluon Je gil cDncordoat vaue

5 Lnleulate the ameLi c a l n in ke QULeenomele

Reult he conlent calcum n mulk_peuden u

O6q/L
Porecaoy
1 1aien sheu e cnree cl alpH[Oat_uelult i
ince seeceesralira aEDTA
2 Handle all othoraliu uuth ta
 OlueeuLon
- mut af o.l M NaOH sequeed da. neclbualie Som Mg-EDTA.
TA
Melwwe a 0.iM EDTA gulion used in dlution
I niliaReadmg Fmal Readmg Valume 0.1 EDTA
17,4 19. 12. 44
17.5 14.S 17/5 4.5
.54.5
15 md
Concdeet wal.

M,V, M2V,
M s0 = (19.5Xo.0)
-e03GM¢O0 29 M
M2
O.0029 x40
0.003540:0
O.Oq/L O.l16 q/
 O- CH
NCH-CH, N
CH 0 [EDTA]
0-C-CH2

M Metal EOTA pler

M= Ca

,OH OH

os N=N-
NO2
BJack -T

RRCa -
(a+EDTA Ca EDTA
MaI EDTA Mg EDTA+In
Jalune)