Tsiala Bayakala

Jordan Agustin

Symia Shelton

03-07-2023

BIOL-1441-2232

Chaturi Devmika

Group Assignment: Osmosis Results and Discussion

Osmosis Results
12

10
Change in mass(g) 159
Change in mass (g)

8 mM NaCl
6 Change in mass (g) 4 M
NaCl
4
Linear (Change in
mass(g) 159 mM NaCl)
2
Linear (Change in mass
0 y = -0.4629x
-0.4486x + 5.9048
5.7143 (g) 4 M NaCl)
0 5 10
R² = 0.4109
0.4169 15 20
-2
Time (min)

Time (min) Change in mass(g) 150 mM NaCl Change in mass(g) 4 M NaCl

0 10.3 10.7
3 0.6 0.5
6 0.7 0.9
9 1.1 0.9
12 0.6 0.8
15 0.8 0.8

When taking a look at the results of the change in mass of the 150 mM NaCl solution and the
change of mass of the 4 M NaCl solution, our group saw that there was only a small difference
in the numbers through each time segment. In the beginning of the trials, both solutions had a
starting mass between 10.0 and 11.0. As we checked the solutions through each interval, we
noticed the 4M NaCl solution had a predominately higher mass than the 150 mM NaCl solution.
For the R^2 values, although it looks a little confusing on our figure, we found that the 4M
solution had a higher R^2 value of 0.4169 than the 150mM NaCl R^2 value of 0.4109. This
showed us that there was no close relationship between the variables. Although this may be
true, when taking a look at our figure we saw that the trends between both solutions results
were pretty close in numbers. We assume that with more time intervals or trials, we would
start to see the relationship between the two variables.

Osmosis is where solvent molecules can move through semi-permeable membrane to create an
equal amount of concentration. In diffusion, molecules move from a place in the cell of higher
concentration to lower concentration to make an equal amount of concentration. In our lab, we
mimicked three different situations on how osmosis and diffusion work. In the first part, we
used poker chips to different molecules (water, sucrose, glucose, and starch) move in and out
of a cell either as a hypertonic, hypotonic, or isotonic solution. This was to gain a basic
understanding of how the molecules move through a cell in the different solutions. In the
second part, we observed how osmosis happens. This compared how a normal cell and a cystic
fibrosis cell molecules transport molecules. In the experiment, the normal maintained a steady
level of molecular movement where as in the cystic fibrosis, when it had water come it, it held
all the water and never released any. In the last part of the lab, we experimented how diffusion
occurs in the cell. The dialysis tubing was filled with NaCl (salt) and water combination. This
mimicked how in higher concentrations, how a cell lets molecules leave hypotonically.
In these experiments, we observed that when the processes stopped transporting
molecules in the different processes, the concentration of each were equal. In Eli Meir tested
his experiments with his students, he found that in both osmosis and diffusion, the molecule
movement stops once the concentration level was equal both in the cell and the water
molecules (Meir, 2017). He explains that there is no more reaction within the molecule. In our
tube bags in the beakers, we observed the the salt water slowly mixed into the water with the
normal cell but stayed in the cell in the cystic fibrosis cell a lot more.