Teacher: Karem Vidaurri Subject: Principles of Biomedical Science Date: 04/17/2023

SLE/Objective Lesson Title Materials

3.1.6 Study and manipulate
microorganisms to
Bacteria Culturing and Gram 

Petri dish (with bacteria)
Q-tips
understand properties Staining  Inoculation loop
(growth and behavior) and  Ethanol
their role in infectious Warmup/Bell Activity  Iodine
diseases Students will look at the bacteria they  Water
Unit have cultured and determine their  Crystal Violet
shape and elevation on their lab  Safrin
Unit 3: Outbreaks and worksheet.  Pipettes
Medical Emergencies
 Microscope Slide
Vocabulary  Safety googles.
- Gram positive  Gloves
-Gram Negative
-Bacteria Proactive
-Petri dish Students will have to gram stain their
-Incubate bacteria to determine what the bacteria
-Bacilis is.
-Cocci
-Spirilla

Activities
Students will be allowed to go around the school collecting bacteria from different areas in the school. They
will swab the desired area with a Q-Tip for 30 seconds, then swab their bacteria on their petri dish and
incubate the bacteria for 3 days.
Students will look at their petri dish and describe/draw the way it looks in their lab notebook. Students will
now go through a step-by-step process on how to gram stain their bacteria. After they gram-stain their
bacteria they will place their slide under a microscope and determine what bacteria is growing around the
school.

Instructional Strategies Assessment

 Students will be in their table groups. Students will have to prepare their microscope slide through gram
 Students will have 10 minutes to go staining and be able to determine the bacteria by looking at it
around the school and swab for bacteria under a microscope.
samples.
 Students will prepare their petri dish and
incubate their bacteria for 3 days.
 Students will observe the bacteria they
cultured and determine the shape and
elevation.
 Students grab some bacteria from their
petri dish with their inoculation loop and
place it on their microscope slide.
 Students will now begin the gram staining
process.

Notes