Lab MANUAL PCOS 2023

PCOS311- COSMETIC PRODUCT DEVELOPMENT LABORATORY MANUAL
OUR LADY OF FATIMA UNIVERSITY

COLLEGE OF PHARMACY
LABORATORY MANUAL
IN
COSMETIC PRODUCT DEVELOPMENT
2022
LABORATORY SAFETY RULES AND REGULATIONS
1. Laboratory safety information and procedures must be read before using equipment or
conducting an experiment. Instructions should be followed in carrying out the activity or
investigation.
2. Working in the laboratory without the supervision of the instructor is prohibited.
3. Unauthorized activities or investigations are prohibited.
4. The chemical storage area is prohibited to unauthorized personnel.
5. Removing chemicals or equipment from the laboratory is prohibited unless authorized.
6. All accidents, chemical spills and injuries, no matter how trivial they may seem at the time, must
be reported immediately to the instructor.
7. Hair ties should be used by students when necessary to prevent laboratory accidents. Moreover,
students wearing dangling jewelry, acrylic nails, sandals and open-toed shoes are not allowed
inside the laboratory.
8. Location of all safety equipment (e.g. emergency shower area, fire extinguishers and fume hood)
present in the room should be known.
9. Laboratory gloves should be worn throughout the activity when necessary.
10. Precautions should be practiced when using flame-producing devices or heating equipment.
Never leave any fire unattended.
11. Bags and books should remain in an area designated by the instructor.
12. Sitting on the laboratory tables is prohibited.
13. Work areas should be kept clean and neat at all times. Work areas should be cleaned at the end
of the laboratory period.
14. Glassware should be washed with warm water and liquid detergent. They should be rinsed, dried
and returned every after the laboratory period.
15. Hot glassware should not be immersed in cold water.
Handling of Chemicals
1. The reagent bottle labels should be read twice before using. The student should be certain that
the reagent used is the correct one.
2. The reagent bottle should be immediately covered after using it. The reagent should be at its
designated location at all times.
3. Unused chemicals should not be returned to the reagent container. Directions for the storage or
disposal of these chemicals should be followed.
4. Solid chemicals, metals, matches, filter papers, broken glasses and other materials should be
disposed in their proper waste containers, not in the sink.
5. Proper disposal of chemical waste should be practiced at all times. Mixing of chemicals in the
sink should not be practiced.
6. All chemicals in the laboratory should be considered poisonous. Chemicals should not be
handled with bare hands. Chemicals should not be smelled directly.
I have read and understood the laboratory safety rules and regulations. I acknowledge that
these rules are necessary to prevent accidents and to ensure my own safety and of others. Moreover, I
will follow any additional instructions given by my instructor. I understand that I should ask my instructor at
any time about the instructions, rules and regulations if they are not clear to me. I agree to follow and
abide to these rules and regulations in the laboratory.
Conforme:
_________________________ _________________ ________________________

Student Signature Subject Instructor
(Signature over printed name) (Signature over printed name)
_________________________
Date
---------------------------------------------------------------------------------------------------------------------
(To be submitted to the instructor)
Conforme:
_________________________ _________________ ________________________

Student Signature Subject Instructor
(Signature over printed name) (Signature over printed name)
_________________________
Date
First Aid in the Laboratory
ALL accidents, injuries, and spills should be reported immediately to the instructor.

ALL STUDENTS should know the following:

 Laboratory Safety Techniques
 Procedure in reporting an accident, injury, or spill
 Location of first aid kit, fire alarm, fire extinguisher and emergency shower
 Location of emergency escapes
Basic First Aid Procedures for Laboratories
Situation Safe Response
Burns Minor – include small scalds or burns from hot objects.
 Flush the burned area with cool water from the faucet or use
cool wet compresses to the skin.
 Cleanse the burned area and apply burn cream from the first
aid kit.
Chemical Burn
 Start treatment immediately by placing the burned area under

cool running water for at least 15 minutes.
 If the chemical reached the eye, irrigate the injured eye with
cool water for at least 15 minutes. Make sure that the water will
not run into the other eye. Then, cover the eye with a sterile
compress.
 Recommend the individual to seek emergency medical
treatment.
Cuts & Bruises  Treat as directed using instructions included in the first aid kit.
Fire  Turn off all flames and gas jets, and then wrap the person on
fire with wet cloth.
 Use fire extinguisher to put out the fire, DO NOT use water.
Foreign Matter in  Irrigate the injured eye with running water for 15 minutes.
Eyes  Seek medical help.
Poisoning  Note the suspected poisoning agent.

 Consult the instructor for the appropriate antidote.
 Call the nearest poison control center if more help is needed.
Severe bleeding  Apply direct pressure to the source of bleeding.

 Seek medical help immediately.
Chemical spills,  Wash the area with running water, use safety shower.
Acid burns,  For acid burns, apply sodium bicarbonate (baking soda).
Base burns  For base burns, apply boric acid.
USING THE FIRE EXTINGUISHER
1. Pull the Pin at the top of the extinguisher. The pin releases a
locking mechanism that will discharge the extinguisher.
2. Aim at the base of the fire, not the flames. In order to put out
the fire, the fuel must be extinguished.
3. Squeeze the lever slowly. This will release the extinguishing

agent in the extinguisher. If the handle is released, the discharge will
stop.
4. Sweep from side to side. Using a sweeping motion, fire

extinguisher must be moved back and forth until the fire is completely
out. Operate the extinguisher from a safe distance, several feet away,
and then move towards the fire once it starts to diminish.
TABLE OF EXPERIMENTS
Experiment No. Title Page No.

1 Overview of Cosmetic Science
2 Preparation of Bar Shampoo 16
3 Preparation of Hair Conditioner 19
4 Preparation of Skin Lotion 23
5 Preparation of Body Wash
6 Preparation of Breath Spray 27
7 Preparation of Lip Tint 30
8 Preparation of Face Powder 32
Preparation of Perfume
9 35
SUMMARY OF RUBRIC SCORES
Experiment No. Title Score.

1 Overview of Cosmetic Science
2 Preparation of Bar Shampoo
3 Preparation of Hair Conditioner
4 Preparation of Skin Lotion
5 Preparation of Body Wash
6 Preparation of Breath Spray
7 Preparation of Lip Tint
8 Preparation of Face Powder
Preparation of Perfume
9
TOTAL:
The laboratory comprises 40% of the total grade
20% Quizzes/ Practical/Performance

20% Long exam (prelim) 15% Manual
20% Long exam (midterm) 5% Attendance
20% Long exam (finals)
Practical tests/ Performance and the manual will be graded using the rubrics.
Beginning Checklist:
Required PPE:
Laboratory Gown
Head cap
Face mask
Gloves
Calculator
Syringe (1ml)
White table Mantle
Wiping/ cleaning Rags
Tissue paper
Plastic tray
Plastic stirrer
Parchment/ wax paper
Labels
White and red colored paper
Markers and pens
Masking tape
Bond paper
Glue and pair of scissors
GENERAL NOTES IN COSMETIC PRODUCT DEVELOPMENT
Cosmetics and personal care products are applied to the human body for the purposes of cleaning,
beautifying, promoting attractiveness or changing its appearance.
Ranging from antiperspirants, fragrances, make-up and shampoos, to soaps, sunscreens and
toothpastes, cosmetics and personal care products play an essential role in in all stages of our life.
Specifically, there are seven categories of cosmetics and personal care products - oral care, skin
care, sun care, hair care, decorative cosmetics, body care and perfumes. Further examples are listed in
the diagram below.
Ficheaux et al., 2015

ILLUSTRATIVE LIST BY CATEGORY OF COSMETIC PRODUCTS

 - Creams, emulsions, lotions, gels and oils for the skin (hands, face, feet, etc.).
 - Face masks (with the exception of chemical peeling products).
 - Tinted bases (liquids. pastes, powders).
 - Make-up powders, after-bath powders, hygienic powders, etc.
 - Toilet soaps, deodorant soaps, etc.
 - Perfumes, toilet waters and eau de Cologne.
 - Bath and shower preparations (salts, foams, oils. gels, etc.).
 - Depilatories.
 - Deodorants and anti-perspirants.
 - Hair care products.
- hair tints and bleaches.
- products for waving, straightening and fixing,
- setting products,
- cleansing products (lotions, powders, shampoos),
- conditioning products (lotions, creams, oils),
- hairdressing products (lotions, lacquers, brilliantines).
 - Shaving products (creams, foams, lotions, etc.).
 - Products for making-up and removing make-up from the face and the eyes.
 - Products intended for application to the lips.
 - Products for care of the teeth and the mouth.
 - Products for nail care and make-up.
 - Products for external intimate hygiene.
 - Sunbathing products.
 - Products for tanning without sun.
 - Skin-whitening products.
 - Anti-wrinkle products.
A "cosmetic product" shall mean any substance or mixture intended to be placed in contact with the
various external parts of the human body (epidermis, hair system, nails, lips and external genital organs)
or with the teeth and the mucous membranes of the oral cavity with a view exclusively or mainly to
cleaning them, perfuming them, changing their appearance and/or correcting body odours and/or
protecting them or keeping them in good condition.
As a general rule, cosmetic products must only make cosmetic claimed benefits; and not medicinal or
therapeutic claimed benefits. Any cosmetic claimed benefits made shall be aligned with what is accepted
internationally and shall be justified either by technical data and/or cosmetic formulation or preparation
itself. Manufacturers / product owners will be allowed to use their own scientifically accepted protocols /
design in generating the technical data provided there is justification why such protocol / design is used.
FORMULA DEVELOPMENT
1. Idea is identified and described by Marketing in a product brief
The brief summarizes product characteristics like product form, appearance, fragrance, benefits, claims,
competitive products, packaging characteristics, price range, geographical market, etc.
2. Marketing and R&D meet to review and refine the brief

R&D helps define the specifications and determine what is technically feasible from a production and cost
perspective.
3. R&D researches the product

Using the information in the product brief, ingredients are researched from a technical, regulatory, and
price perspective to create a "dry lab" formula, or a list of ingredients and percentages.
4. R&D submits paper formula to Marketing

Before laboratory work begins, Marketing reviews the paper formula to make sure both parties are on the
same page regarding cost, ingredients, and claims. This is the time to confirm requirements for
ingredients like an extract that must be included at a certain percentage or a preservative that cannot be
used.
5. R&D makes prototypes

R&D begins laboratory work to produce prototypes and test for functionality, safety, aesthetics, stability,
and packaging compatibility.
6. Marketing reviews the prototypes and provides feedback

Marketing gives feedback to the technical team about changes they would like to see in the formula. This
step iterates until the formula is finalized. The more specific the feedback, the easier it is for R&D to
change.
For example: "The lotion is too thick" is not as helpful as "The lotion is too thick to easily dispense from
the tube. It should be less viscous but not so thin that it leaks from the package orifice"
FORMULA TESTING
7. Consumer testing
This step can be expensive if you are testing a new product with a large group of consumers. The idea is
to get consumer insights on product attributes. The level of testing could range from informal feedback
from company employees to a focus group of consumers to in-home testing of prototypes. In some cases,
consumer testing will be skipped completely.
8. Stability testing
The most important R&D testing is stability. Will the product change over time or interact with the
packaging in a way that is undesirable? (Examples include separation, color change, change in pH or
viscosity, degradation of preservatives or active ingredients) The results of stability testing determine the
product shelf life.
9. Clinical studies
Clinical testing is typically done to verify a specific claim, such as non-comedogenic. Other testing may be
necessary depending on the formula type. For example, sunscreen products need to be tested to confirm
the SPF level.
COMMERCIALIZATION
10. Scale-Up
This is where the process engineer takes over from the cosmetic chemist. While the chemist made
prototypes in a beaker at approximately one-kilogram scale, the engineer will scale the product up to its
full manufacturing size which could be in the range of 500 to 20,000 kg. The process typically involves a
mid-size pilot batch, a full-size trial batch, and if all goes well, the first commercial manufacturing batch to
be sold to consumers. The best analogy is taking a cooking recipe designed for a small family and
upgrading it for a large event.
11. Release testing

Before the first commercial batch can be sold, it will be tested against the specification created by R&D.
The specification includes tests for appearance, color, odor, pH, viscosity, and levels of preservatives and
active ingredients.
12. Ship to trade

If all testing passes, the product is released to market and shipped to customers. Success!
Marketing and R&D could be two departments in the same large organization, but other variants exist. For
a small brand, Marketing might be the founder and R&D could be the contract manufacturer making the
products. Even for large companies, some steps may be outsourced such as clinical testing and stability
testing.
This process is complicated by additional stakeholders. Upper management may have a stage-gate
process where cost and progress are reviewed after key milestones. Projects can be cancelled at any
time if they are over budget, taking too long, or if the retailer decides not to take the new formula.
In addition to Marketing and R&D, many other departments provide input including: Quality,
Manufacturing, Supply Chain, Sales, Procurement, Packaging Engineering, Microbiology, Analytical,
Project Management, Demand Forecasting, etc.
DEFINITION OF TERMS
Agitation is to stir in a rapid or brisk motion
Beyond-use date is the date after which an article shall not be used. An official compounded preparation
shall bear a beyond-use date.
Caustic is any topical drug that destroys tissue on contact, useful in removing skin lesions.
Comminute is to reduce the particle size by using mortar and pestle.
Congeal is the change of a soft or liquid to rigid or solid consistency.
Container is that which holds the article and is or may be in direct contact with the article. It may be made
up plastics and glass. Plastics are of different types: Polyethylene (PE), High density or low density is
suitable for packaging dry oral dosage forms. Polyethylene Terephthalate (PET) is suitable for packaging
liquid oral dosage form. Polypropylene (PP) is suitable for packaging dry solid or liquid oral dosage form.
Corrosive is any substance that can cause obvious damage to living tissue.
Decant is the process of separating particulates from a liquid by allowing the solids to settle down at the
bottom of the mixture.
Deliquescent is to dissolve and become lliquid by absorbing moisture from the air.
Demulcent is a bland viscous liquid used to coat and soothe the damaged or inflamed skin or mucous
membrane.
Effervescence is foaming formed as a result of a gas being evolved from a solid or liquid.
Efflorescent is the process of losing water of hydration from a hydrate.
Emollient is a substance applied to the skin to make it smooth and soft.
Expiration date gives the date during which the article may be expected to meet the requirements of the
compendial monograph, provided it is kept under the prescribed storage conditions.
Hygroscopic is one that readily absorbs water (usually from the athmosphere).
Immediate packaging means the container or other or other form of packaging immediately in contact with
the cosmetic product
Ingredient is any material or component which maybe an active or inactive incorporated in a preparation.
Label is the part of the labeling upon the immediate container.
Labeling is the term used to designate all labels and other written, printed, or graphic matter upon an
immediate container of an article or upon, or in, any package or wrapper in which it is enclosed, except
any outer shipping container
Levigate is to reduce a substance to a fine powder using a levigating agent (usually glycerin).
Name of the cosmetic product means the name given to a cosmetic product, which may be an invented
name, together with a trade mark or the name of the manufacturer;
Outer packaging means the packaging into which is placed the immediate packaging;
PPE (Personal Protective Equipment) refers to protective clothing, helmets, goggles, or other garments or
equipment designed to protect the wearer's body from injury.
Preparation is a drug dosage form, a dietary supplement, or a finished device. It is the finished or partially
finished preparation of one or more substances formulated for use on or for the patient or consumer.
Slake is to moisten or to undergo the process in which the lime reacts with water.
Triturate is to crush, rub or grind usually with the use of mortar and pestle.
Research is aystematic study of something (information, behavior) in order to establish facts, insights
and conclusions.
Research and Development — In a product company, typically the team tasked with identifying new
technology, performance, or value and applying these to products.
EXERCISE NO. 1
OVERVIEW OF COSMETIC SCIENCE
OBJECTIVE/S:
The students are expected to:
 Demonstrate familiarity on cosmetic science terminologies.
 Describing the GMP requirements for cosmetics.
 Identify the requirements for cosmetic notification.
What is Cosmetics?
Cosmetics means any article intended to be rubbed, poured, sprinkled or sprayed on, or introduced into,
or otherwise applied to, the human body or any part thereof for cleansing, beautifying, promoting
attractiveness, or altering the appearance, includes any article intended for use as a component of
cosmetic.
What is Cosmetic Science?

Cosmetic science is the study of the effects that raw materials and mixtures can have on parts of the
human body like hair, skin, lips and nails. The subject of cosmetic science actually overlaps with a
number of sciences including Chemistry, Physics and Biology but also includes softer topics like
Marketing and Market Research.
Terminology:
1. Define the following:
a) Aesthetics
b) AHA
c) Allergenicity
d) Biotin
e) Ceramides
f) Co-emulsifier
g) Comedone
h) FD & C
i) Keratinization
j) Melanogenesis
Questions:
1. What are the FDA labeling requirements for the marketing of cosmetics (lips, hair, face and
body) in the Philippines? Create a tabulation of comparison.
2. What are the classification of 'Cosmetic Claims'? Give examples of each.
3. Give a brief description of the status of cosmetic science today.

Name: ____________________ Date: _________________

Group No.: ____________________ Yr and Sec: ___________
Preparation: ____________________
ASSESSMENT RUBRIC FOR COMPOUNDING LABORATORY

Criteria Exemplary Proficient Good Needs Unacceptable Score/
(5) (4) (3) improvement (1) Comments
(2)
ACCURACY & Exemplary Proficient Good Needs Unacceptable
COMPLETENE (5) (4) (3) improvement (1)
SS (2)
The needed
information in
each question
of the
assignment is
accurate and
complete
PRECISE Exemplary Proficient Good Needs Unacceptable
VOCABULARY (5) (4) (3) improvement (1)
Vocabulary (2)
and medical
terms are used
appropriately in
all the answer
in the
assignment.
REFERENCE/ Exemplary Proficient Good Needs Unacceptable
SOURCE (5) (4) (3) improvement (1)
The reference/ (2)
source are all
cited in an
accurate APA
format.
TIMELINESS Exemplary Proficient Good Needs Unacceptable
OF (5) (4) (3) improvement (1)
SUBMISSION (2)
The
assignment is
submitted on
time.
TOTAL
EXPERIMENT 2
PREPARATION OF SOLID SHAMPOO
Solid shampoo bars are usually manufactured by small companies that focus on a limited line of all-
natural goods. Manufacturers produce shampoo bars by concentrating cleansing and conditioning agents
into bars, then removing all
OBJECTIVE/S:
 To formulate a solid bar shampoo
Warning: Always wear your protective gear when using Lye.

Material/s:
Raw materials Quantity for 5g Computed amount for 10 g
Olive Oil 1mL
Palm Kernel Flakes 0.75 mL
Sweet Almond Oil 0.4 mL
Lye 3.12 g
Castor oil 0.4 mL
Essential Oil 0.01 mL
Procedure:
1. Combine olive oil, palm flakes, sweet almond oil, and castor oil in a water bath and melt over
medium/low heat. Remove from heat and allow to cool to 100 degrees Fahrenheit.
2. Wearing proper safety gear including gloves and a mask, pour lye crystals into water and stir until
dissolved. Allow to cool to 100 degrees Fahrenheit. If oils or lye water are not cooling at the same
rate, use a cold water bath in the sink.
3. When both lye water and oils are at the correct temperature, pour lye water into oils and mix with
a hand or stick mixer until medium trace.
4. Add drops of essential oil and blend well.
5. Pour into mold and insulate for 24 hours.
6. Remove from mold and cut.
7. Allow to cure for 24hrs in a cool, non-humid location.
------------------------------------------Show your computation below: ------------------------------------------------------

Computation:
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXPERIMENT 2 SOLID SHAMPOO
QUESTIONS:
1. What are the FDA regulatory requirements to market bar shampoo in the Philippines?
2. Enumerate the materials used in the experiment and Identify the use of each.
3. List down the advantage and disadvantage of using bar shampoo instead of the traditional one
4. In your own opinion, What are the reasons why some people use their own organic shampoo
formula?
Name: ____________________ Date: _________________

Preparation: ____________________

(2)
PROCEDURE. Exemplary Proficient Good Needs Unacceptable
Demonstrates (5) (4) (3) improvement (1)
understanding of the (2)
compounding
procedure with no
assistance needed.
Works in a clean and
organized manner
practicing safety
measures at all times
LABEL. Complete Exemplary Proficient Good Needs Unacceptable
and accurate (5) (4) (3) improvement (1)
information. Correct (2)
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned
PRODUCT. Correct Exemplary Proficient Good Needs Unacceptable

product description (5) (4) (3) improvement (1)
Correct product (2)
amount in proper
container
TIMELINESS OF Exemplary Proficient Good Needs Unacceptable

SUBMISSION (5) (4) (3) improvement (1)
The assignment is (2)
submitted on time.
TOTAL
1.
EXPERIMENT 3
HAIR CONDITIONER
A hair conditioner is an ingredient or product that when applied to hair according to its recommended us,
procedure and concentration improves manageability, gloss and smooth touch of the hair. When using
shampoos containing anionic surfactants it leaves the hair difficult to comb while wet. It also results in a
static charge build-up or fly away when the hair is combed dry.
Most of the shampoos contain conditioning agents, but consumers often apply a conditioner after
shampooing, to minimize hair frizzing, increasing manageability and gloss of the hair. A conditioner can
therefore be used to reduce a chemical or mechanical trauma of the hair, such as permanent dyes,
bleaching, and excessive brushing. The hair shaft has negative electrical charges, while conditioning
agents have positive charges: conditioners acts by neutralizing the electrical negative charge of the hair
fiber by addicting positive charges and by greasing the cuticle, with the final result of reduce fiber
hydrophilicity. Conditioners also help the raised cuticles to lie down against the hair surface, and in this
way preserve hair color and enhance its shyness and smoothness too.
OBJECTIVE/S:
 To know the formulation and procedure in making hair conditioner.
Raw materials Quantity for 30mL Computed amount for 10 mL

Citric acid 1.35 mg
Cetyl Alcohol 162 mg
Stearyl Alcohol 108 mg
Cetrimonium Chloride, 25% 325.8 mg
Glyceryl Stearate 18 mg
DMDM Hydantoin 18 mg
Disodium EDTA 2.7 mg
Methyl Paraben 2.7mg
Fragrance 0.5 mL
Water qs ad 30 ml
Procedure:
1. Deionized water, citric acid, and disodium EDTA were placed in an appropriately sized vessel and
heated, with stirring, to 80 °C.
2. When the aqueous solution had reached 70°C, cetyl alcohol, stearyl alcohol, and glyceryl stearate were
added.
3. The mixture was heated with stirring until the temperature reached 80°C and then the temperature was
maintained for 10 minutes.
4. The mixture was then cooled to 60 °C and cetrimonium chloride (25%) was added.
5. The mixture was then cooled to 40 °C and DMDM hydantoin, preservative and fragrance were added.

Computation:
Name:_____________________________________ Date:______________________
Section:___________________________________ Score:______________________
EXERCISE 3
HAIR CONDITIONER
QUESTIONS:
1. Identify the use of the following raw materials in the hair conditioner formulation.
MATERIALS USES
Citric acid
Cetyl Alcohol
Stearyl Alcohol
Cetrimonium Chloride, 25%
Glyceryl Stearate
DMDM Hydantoin
Disodium EDTA
Methyl Paraben
Fragrance
Water
2. What are the FDA regulatory requirements to market hair conditioner in the Philippines?
3. What are the Philippine FDA labelling requirements in hair conditioner ?
4, Provide a sample label of hair conditioner approved by FDA.

Name: ____________________ Date: _________________

Preparation: ____________________

(2)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned

Correct product (2)
amount in proper
container

submitted on time.
TOTAL
EXPERIMENT 4
SKIN LOTION
Skin lotions are liquid suspensions or dispersions intended for external application; some consist of finely
powdered, insoluble solids held in more or less permanent suspension by suspending agents or surface-
active agents, or both; others are oil-in-water emulsions stabilized by surface- active agents.
OBJECTIVE/S:
 To formulate a skin lotion
Distilled monoglycerides 1g
Stearic acid 1g
Cetyl alcohol 0.75g
Lanolin 0.5g
Petroleum jelly 0.5g
Glycerin/Propylene glycol 2g
Mineral oil 3g
Triethanolamine 2g
Methyl paraben 0.05 g
Propyl paraben 0.05 g
Lavander oil 0.5 mL
Distilled water to make 50 mL
Procedure:
1. Measure all the ingredients separately in a top loading balance.
2. In a container, combine all the oil phased ingredients: distilled monoglycerides, stearic acid cetyl
alcohol, lanolin, petroleum jelly, mineral oil, glycerin or petroleum jelly, triethanolamine methyl paraben
and propyl paraben. 3. In another container, put the prescribed amount of distilled water.
4. Cover both containers with aluminum foil.
5. Heat both containers in water bath for 10 to 15 minutes at 70 degrees Celsius to 75 degrees celsius
until all ingredients have melted.
6. Once done, add the melted oil phased ingredients to the heated aqueious ingredient while stirring
continuously until an emulsion is formed. 7. Allow the emulsion or the mixture to cool down to 60 degrees
Celsius and homogenize for 15 to 20 minutes until smooth texture is attained.
8. Add scent and mix thoroughly.
9. Use a stirring rod to transfer the mixture to your hand and body lotion containers.

Computation:
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXPERIMENT 4 SKIN LOTION
2. What are the FDA regulatory requirements to market a skin lotion in the Philippines?
3. What are the Philippine FDA labelling requirements in skin lotion ?

Name: ____________________ Date: _________________

Preparation: ____________________

(2)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned

Correct product (2)
amount in proper
container

submitted on time.
TOTAL
EXPERIMENT. 5
BODY WASH
Personal care composition products like body washes are becoming more popular in the
United States and around the world. Desirable body wash compositions must meet a number of
criteria. For example, in order to be acceptable to consumers, a body wash composition must
exhibit good cleaning properties, must exhibit good lathering characteristics, must be mild to the
skin (not cause drying or irritation) and preferably should even provide a conditioning benefit to
the skin.
Body wash compositions that attempt to provide skin-conditioning benefits are known.
Many of these com positions are aqueous Systems comprising emulsified conditioning oil or
other similar materials in combination with a lathering Surfactant. Although these products
provide both conditioning and cleansing benefits, it is often difficult to formulate a product that
deposits sufficient amount of skin conditioning agents on Skin during use. In order to combat
emulsification of the skin conditioning agents by the cleansing Surfactant, large amounts of the
skin conditioning agent are added to the compositions. However, this introduces another
problem associated with these cleansing and conditioning products. Raising the level of Skin
conditioning agent in order to achieve increased deposition negatively affects product lather
performance and stability.
OBJECTIVE/S:
 To formulate a solid body wash

Raw materials Quantity for 100 mL Computed amount for 20 mL
Coconut Oil 60mL
Honey 60mL
Liquid Castile Soap 118 mL
Vitamin E 5 mL
Eucalyptus Essential Oil 0.5 mL
Sweet Orange Essential Oil 0.5 mL
Lemon Or Grapefruit Essential 0.5 mL
Oil
Procedure:
1. Scoop the coconut oil into a microwave-safe bowl and heat on a medium setting for 30 seconds until
melted.
2. Add essential oils, honey, and vitamin E oil and whisk together.
3. Add Castile soap slowly, stirring gently to avoid creating suds.
4. Transfer to a glass or plastic bottle. Shake before each use.

Computation:
Name:_____________________________________ Date:______________________
Section:___________________________________ Score:______________________
EXERCISE 5
BODY WASH
QUESTIONS:
1. Identify the use of the following raw materials in body wash formulation.
2. What are the FDA regulatory requirements to market body wash in the Philippines?
3. What are the Philippine FDA labelling requirements in body wash?
4, Provide a sample label of hair conditioner approved by FDA.

Name: ____________________ Date: _________________

Preparation: ____________________

(2)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned

Correct product (2)
amount in proper
container

submitted on time.
TOTAL
EXPERIMENT 6
BREATH SPRAY
Breath spray is a product sprayed into the mouth for the purpose of temporarily eliminating or at least
covering up bad breath. The masking effect is short-term and reported to last for 4-6 hours. The
composition is provided which may be in the form of a comestible, such as a pill, powder, tablet, pressed
candy or mint, boiled candy, mouthwash, dental cream, chewing gum and the like, and which includes as
its active ingredient calcium hydroxide alone or combinations of calcium hydroxide and magnesium
hydroxide or combinations of magnesium hydroxide and/or calcium hydroxide with copper gluconate. In
addition, a method is provided for inhibiting bad breath wherein calcium hydroxide alone or any of the
above combinations is employed.
OBJECTIVE/S:
 formulate a Breath spray
Raw materials Quantity for 30mL Computed amount for 20mL

vegetable glycerin 5 mL
Xylitol 1 mL
Peppermint oil 1.5 mL
Cinnamon essential oil 1mL
Castor oil 0.4 mL
Clove oil 0.5 mL
Distilled water to make 30 mL
Procedure:
1. Measure all the ingredients separately in a top loading balance.
2. Combine all the ingredient in a glass beaker.
3. Use a funnel to transfer into your bottle.


Computation:
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXPERIMENT 6
BREATH SPRAY
QUESTIONS:
2. What are the FDA regulatory requirements to market a breath spray in the Philippines?
3. What are the Philippine FDA labelling requirements for breath spray or other oral products ?
COSMETIC PRODUCT DEVELOPMENT LABORATORY
_________________________________________________________________________________________________________________
Name: ____________________ Date: _________________

Preparation: ____________________

Criteria Exemplary Proficient Good Needs Unacceptabl Score/
(5) (4) (3) improvement e Comments
(2) (1)
PROCEDURE. Exemplary Proficient Good Needs Unacceptabl

Demonstrates (5) (4) (3) improvement e
understanding of the (2) (1)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
LABEL. Complete Exemplary Proficient Good Needs Unacceptabl
and accurate (5) (4) (3) improvement e
information. Correct (2) (1)
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned
PRODUCT. Correct Exemplary Proficient Good Needs Unacceptabl

product description (5) (4) (3) improvement e
Correct product (2) (1)
amount in proper
container
TIMELINESS OF Exemplary Proficient Good Needs Unacceptabl

SUBMISSION (5) (4) (3) improvement e
The assignment is (2) (1)
submitted on time.
TOTAL
2.
1
_________________________________________________________________________________________________________________
Exercise no. 7
LIP TINT PREPARATION
Pigments from plant products can be combined with a cosmetic carrier formulation to create a
cosmetic and/or dermatological product for transferring color to the skin of the person. A plant product
extract or reformulation, such as a concentrate, flake or powder of the plant, can be combined with a
cosmetic carrier formulation to create a cosmetic and/or dermatological product for transferring color to
the skin of the person. In some embodiments, fruits, vegetables, seeds and legumes can be used to
extract natural pigment or reformulate a plant product into a concentrate, flake or powder. Resultant
cosmetic products can include lipstick, lip gloss, lip stain, lip liner, blush, face tint, cheek stain, cheek gel,
cheek butter, eye shadow, eyebrow powder, eyeliner, mascara, foundation, sheer foundation, bronzer,
facial illuminator, facial highlighter, face powder, lotion and tinted moisturizer.
A variety of coloring agents are typically used to color cosmetics, including inorganic and organic
synthetic dyes or pigments. Many cosmetic manufacturers use artifi cial or man-made pigments approved
by the Food and Drug Administration designated as an FD&C “color followed by a number. Examples of
such pigments include FD&C Blue No. 1, FD&C Blue No. 2, FD&C Green No. 3, FD&C Red No. 3, FD&C
Red No. 40, FD&C Yellow No. 5, FD&C Yellow No. 6, D&C Blue No. 4, D&C Blue No. 9, D&C Green No.
5, D&C Green No. 6, D&C Green No. 8, D&C Orange No. 4, D&C Orange No. 5, D&C Orange No. 10,
D&C Orange No. 11, D&C Orange No. 17, FD&C Red No. 4, D&C Red No. 6, D&C Red No. 7, D&C Red
No. 8, D&C Red No. 9, D&C Red No. 17, D&C Red No. 19, D&C Red No. 21, D&C Red No. 22, D&C Red
No. 27, D&C Red No. 28, D&C Red No. 30, D&C Red No. 31, D&C Red No. 34, D&C Red No. 39, FD&C
Red No. 40, D&C Violet No. 2, D&C Yellow No. 7, Ext. D&C Yellow No. 7, D&C Yellow No. 8, D&C Yellow
No. 11, D&C Brown No. 1, Ext. D&C Violet No. 2, D&C Blue No. 6 and D&C Yellow No. 10.
OBJECTIVE/S:
 To formulate a liptint
Raw materials Quantity for 5g Computed amount for 10 g

FD&C red 0.5g
Vit E 0.5 mL
Aloe vera gel to make 5g
Procedure:
1. Mix the aloe vera gel and food color in a bowl. You can adjust the intensity of the pigment by
adding more food color.
2. Then, puncture a vitamin E capsule and squeeze out its contents to the concoction.
3. Stir in distilled water if the formula is too thick.
4. Blend the ingredients well.
5. Transfer the homemade lip stain to a small ointment jar or lip tint tube

Computation:
2
_________________________________________________________________________________________________________________
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXPERIMENT 7
LIP TINT
Questions:
1. Enumerate and describe other cosmetic colorants.

2. Compare and contrast plant pigments and synthetic colorants in terms of the stability of the product,
formulation, and safety.
3. What are the FDA regulatory requirements for lip tint in the Philippines?

4. What are advantages and disadvantages of using lip tints over lip sticks?
3
_________________________________________________________________________________________________________________
Name: ____________________ Date: _________________

Preparation: ____________________

Criteria Exemplary Proficient Good Needs Unacceptabl Score/
(5) (4) (3) improvement e Comments
(2) (1)
PROCEDURE. Exemplary Proficient Good Needs Unacceptabl

Demonstrates (5) (4) (3) improvement e
understanding of the (2) (1)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
LABEL. Complete Exemplary Proficient Good Needs Unacceptabl
and accurate (5) (4) (3) improvement e
information. Correct (2) (1)
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned
PRODUCT. Correct Exemplary Proficient Good Needs Unacceptabl

product description (5) (4) (3) improvement e
Correct product (2) (1)
amount in proper
container
TIMELINESS OF Exemplary Proficient Good Needs Unacceptabl

SUBMISSION (5) (4) (3) improvement e
The assignment is (2) (1)
submitted on time.
TOTAL
3.
4
_________________________________________________________________________________________________________________
WORKSHEET 8
FACE POWDER
The face powder plays a vital role in the makeup. The powder will give an even look to the skin along with
the foundation. It will be helpful to remove the oiliness of the skin and the patches. Face powder not only
enhance your beauty, but they also have numerous benefits while using like reduces sweat production,
helps to stop body odor and preventing fungal infections.
Objectives:
 To formulate a face powder
Raw materials Quantity for 100g Computed amount for 5

g
Talc 50 g
Mica 15 g
Bentonite 20 g
Magnesium stearate 7.5 g
Calcium carbonate 6.5 g
Inorganic and organic pigments 0.01g
Triclosan 1g

Computation:
PROCEDURE:
1. Mix talc, mica powder, bentonite, magnesium stearate, calcium carbonate in a mortar and pestle.
Set aside
2. Mix dye and triclosan separately.
3. Mix all ingredient and sieve.
4. Transfer to the container and label properly.
5
_________________________________________________________________________________________________________________
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXPERIMENT 8 FACE POWDER

QUESTIONS:
1. Enumerate the materials used in the experiment and identify the use of each.
2. What are the FDA regulatory requirements to market a face powder in the Philippines?
3. What are the inorganic and organic pigments used in face powder? Describe how to adjust to achieve
the face powder color shade.
6
_________________________________________________________________________________________________________________
Name: ____________________ Date: _________________

Preparation: ____________________

(2)
compounding
procedure with no
assistance needed.
organized manner
practicing safety
product use/s and
correct grammar,
spelling, Neat and
attached well
Properly trimmed
and aligned

Correct product (2)
amount in proper
container

submitted on time.
TOTAL
4.
7
_________________________________________________________________________________________________________________
EXPERIMENT 9
PERFUMERY
The word perfume comes from Latin where per means through and fumum means smoke. Initially,

perfumes were used in religious ceremonies, in the form of incense, but eventually, they were used by
people to make themselves smell good.
There are several methods for extracting scents, such as distillation, maceration, expression, and
enfleurage. In distillation, the raw material is heated in a chamber and the fragrant compounds are
recollected through condensation of the distilled vapor. In maceration, the fragrance is extracted by
soaking the raw material in water, oil, or a solvent. The expression technique is a simple one. The raw
materials are simply squeezed or pressed, and the oils are collected. Enfleurage is a two-step process
during which the odor of aromatic materials is absorbed into wax or fat, and then extracted with alcohol.
OBJECTIVE/S:
The students are expected to
 Prepare a perfume from freshly picked flower petals

Fresh flower petals (top note) 3g
cinnamon oil (heart note) 5 mL
Vanilla (base note) 2 mL
Jojoba Oil 9.5 mL
Essential Oil 0.5 mL
Ethanol to make 30 mL

Computation:
Procedure:
1. Using the scissors, cut out pieces flower petals (Rose, Lavender, Honeysuckle, — whatever is easily
available in the area)
2. Soak in ethanol with the popsicle sticks – this will release the aromatic oils from the petals.
3. On a separate container, add jojoba oil
4. Alternatively or in addition to the flower petals, add essential oils in the order of top, middle, and base
notes. For example, rose (top), cinnamon (heart), and vanilla (base). (The typical ratio for top to heart
to base notes is 3:5:2). Add the ethanol to make 30mL
5. Shake the bottle and let it sit in the bottle. Label
6. Though it can be smelled immediately, for best results wait about one week
Name:________________________________ Date:________________________
Section:______________________________ Score:_______________________
EXERCISE 8
PERFUMERY
QUESTIONS:
8
_________________________________________________________________________________________________________________
1. What is the difference between top, heart, and base note?
2. What are the FDA regulatory requirements to market a perfume in the Philippines?
3. What are the Philippine FDA labelling requirements in skin lotion?
4. Enumerate locally produced perfume in the Philippines.
REFERENCES
Achenie L.E.K., Gani R., Venkatasubramanian V. Elsevier; Amsterdam; Boston: 2003. Computer Aided
Molecular Design: Theory and Practice, CACE-12.
Ash M., Ash I. Gower; Aldershot, Hants, England/Brookfield, Vt., USA: 1994. Handbook of Cosmetic and
Personal Care Additives: An International Guide to More than 15,000 Products by Trade Name, Function,
Composition and Manufacturer.
9
_________________________________________________________________________________________________________________
Ansel, Howard et. al., Ansel’s Pharmaceutical Dosage Forms and Drug Delivery Systems,: Lippincott
William and Wilkins, 2011
ASEAN DEFINITION OF COSMETICS AND ILLUSTRATIVE LIST BY CATEGORY OF

COSMETIC PRODUCTS, https://www.aseancosmetics.org/docdocs/technical.pdf
Bagajewicz M.J. On the role of microeconomics, planning, and finances in product design. AIChE
Journal. 2007;53:3155–3170.
Courtney D.L. Emulsifier selection/HLB. In: Rhein L.D., Rieger M.M., editors. Surfactants in
Cosmetics. Marcel Dekker; New York; Hong Kong: 1997. pp. 127–138.
Cussler E.L., Moggridge G.D. Cambridge University Press; Cambridge: 2001. Chemical Product Design.
Douglas J.M. McGraw-Hill; New York, NY: 1988. Conceptual Design of Chemical Processes.
https://cosmeticseurope.eu/cosmetic-products/ Retrieved January 3, 2022.
https://patents.google.com/patent/EP1013261A1/en Retrieved January 3, 2022.
Genaro, Alfonso R. ed. Remington: The Science and the Practice of Pharmacy, Pennsylvania: Mack
Publishing Company, 2013.
Renade, Vasant, Drug Delivery Systems, 3rd Ed., :CRC Press, 2011
Rieger M. Hyaluronic acid in cosmetics. Cosmetics and Toiletries. 1998;113:35–42.
Seider W.D., Seader J.D., Lewin D.R. John Wiley & Sons; New York, NY: 2004. Product and Process
Design Principles: Synthesis, Analysis and Evaluation.
Spiess E. Raw materials. In: Schmitt W.H., Williams D.F., editors. Chemistry and Technology of the
Cosmetics and Toiletries Industry. Blackie Academic & Professional; London; New York: 1996. pp. 1–34.
Shargel, Leon. Comprehensive Pharmacy Review, 5th ed, Philadelphia: Lippincott William and Wilkins
2005.
US Patent. https://patentimages.storage.googleapis.com/fa/8b/44/feb348a5ae99b1/US4112066.pdf,
Retrieved January 3, 2022.
United States Pharmacopoeia Convention Inc. The United States Pharmacopoeia and The National
Formulary. USP 35/NF 30 ed. Pennsylvania: Mack Publishing Company, 2012
10

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Lab MANUAL PCOS 2023

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PCOS311- COSMETIC PRODUCT DEVELOPMENT LABORATORY MANUAL

OUR LADY OF FATIMA UNIVERSITY

LABORATORY SAFETY RULES AND REGULATIONS

LABORATORY SAFETY RULES AND REGULATIONS

_________________________ _________________ ________________________

(To be submitted to the instructor)

LABORATORY SAFETY RULES AND REGULATIONS

_________________________ _________________ ________________________

First Aid in the Laboratory

ALL accidents, injuries, and spills should be reported immediately to the instructor.

ALL STUDENTS should know the following:

Basic First Aid Procedures for Laboratories

Situation Safe Response

Burns Minor – include small scalds or burns from hot objects.

 Start treatment immediately by placing the burned area under

Poisoning  Note the suspected poisoning agent.

Severe bleeding  Apply direct pressure to the source of bleeding.

USING THE FIRE EXTINGUISHER

3. Squeeze the lever slowly. This will release the extinguishing

4. Sweep from side to side. Using a sweeping motion, fire

Experiment No. Title Page No.

SUMMARY OF RUBRIC SCORES

Experiment No. Title Score.

The laboratory comprises 40% of the total grade

20% Quizzes/ Practical/Performance

GENERAL NOTES IN COSMETIC PRODUCT DEVELOPMENT

Ficheaux et al., 2015

ILLUSTRATIVE LIST BY CATEGORY OF COSMETIC PRODUCTS

2. Marketing and R&D meet to review and refine the brief

3. R&D researches the product

4. R&D submits paper formula to Marketing

5. R&D makes prototypes

6. Marketing reviews the prototypes and provides feedback

11. Release testing

12. Ship to trade

Agitation is to stir in a rapid or brisk motion

Comminute is to reduce the particle size by using mortar and pestle.

Congeal is the change of a soft or liquid to rigid or solid consistency.

Efflorescent is the process of losing water of hydration from a hydrate.

Emollient is a substance applied to the skin to make it smooth and soft.

Label is the part of the labeling upon the immediate container.

What is Cosmetic Science?

2. What are the classification of 'Cosmetic Claims'? Give examples of each.

3. Give a brief description of the status of cosmetic science today.

Name: ____________________ Date: _________________

ASSESSMENT RUBRIC FOR COMPOUNDING LABORATORY

Warning: Always wear your protective gear when using Lye.

------------------------------------------Show your computation below: ------------------------------------------------------

EXPERIMENT 2 SOLID SHAMPOO

Name: ____________________ Date: _________________

ASSESSMENT RUBRIC FOR COMPOUNDING LABORATORY

PRODUCT. Correct Exemplary Proficient Good Needs Unacceptable

TIMELINESS OF Exemplary Proficient Good Needs Unacceptable

Raw materials Quantity for 30mL Computed amount for 10 mL

------------------------------------------Show your computation below: ------------------------------------------------------

3. What are the Philippine FDA labelling requirements in hair conditioner ?

4, Provide a sample label of hair conditioner approved by FDA.

Name: ____________________ Date: _________________

ASSESSMENT RUBRIC FOR COMPOUNDING LABORATORY

PRODUCT. Correct Exemplary Proficient Good Needs Unacceptable

TIMELINESS OF Exemplary Proficient Good Needs Unacceptable

------------------------------------------Show your computation below: ------------------------------------------------------

EXPERIMENT 4 SKIN LOTION

3. What are the Philippine FDA labelling requirements in skin lotion ?

_________________ _ ________________

_________________ _ ________________

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _

Name: ____ Date: _