Service Manual TV FC200

High Technology, Inc.
109 Production Road, Walpole MA 02081
Automated Chemistry Analyzer

HTI BioChem FC-200
Service Manual
Document Number : SM-E-FC240

Revision Level : Rev.A
Effective Date : 6/30/2014
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL

Document Number SM-E-FC240
High Technology, Inc. Revision Level Rev.A

109 Production Road, Prepared By I. Litvinova
Walpole MA 02081 Approved By G. Titov
Effective Date 6/30/2014
Service Manual: HTI BioChem FC-200
Contents
1 INSTRUMENT INTRODUCTION .......................................................................................................... 3
1.1 PRINCIPLES AND INTENDED USE .................................................................................................. 3

1.2 MAIN STRUCTURE OF INSTRUMENT ............................................................................................. 3
1.2.1 Sample/reagent disc ............................................................................................................ 4
1.2.2 Sampling system ................................................................................................................. 5
1.2.3 Sampling washing system ................................................................................................... 7
1.2.4 Stirring system ..................................................................................................................... 7
1.2.5 Reaction disc temperature control ....................................................................................... 8
1.2.6 Reaction disc ....................................................................................................................... 8
1.2.7 Reaction Cup ....................................................................................................................... 9
1.2.8 Optical System ..................................................................................................................... 9
1.2.9 Heat emission and dust-proof of the whole machine ........................................................ 10
1.2.10 Display .............................................................................................................................. 10
1.3 TECHNICAL PARAMETERS ............................................................................................................ 10
2 INSTRUMENT INSTALLATION AND CALIBRATION ....................................................................... 12
2.1 INSTRUMENT UNSEALING............................................................................................................. 12

2.2 INSTALLATION ENVIRONMENT ..................................................................................................... 12
2.3 INSTRUMENT CONNECTIONS ....................................................................................................... 13
3 FUNCTIONAL DESCRIPTION ........................................................................................................... 15
3.1 HTI BIOCHEM FC-200 FUNCTION BOX ......................................................................................... 15

3.2 FUNCTION DESCRIPTION OF EACH BOARDS ............................................................................ 18
3.2.1 Main board (MCON) .......................................................................................................... 18
3.2.2 Temperature Control Board (TCON).................................................................................. 21
3.2.3 Photoelectric conversion board (IVAB) .............................................................................. 23
3.2.4 Photoelectric amplification board (ADCB) ......................................................................... 23
3.2.5 Optic-coupler fixing board (OCBD) .................................................................................... 24
3.2.6 Feeding and dispensing liquid alarm board (WSIF) .......................................................... 25
3.2.7 Liquid level detection board (LDCB) .................................................................................. 26
3.2.8 Serial Port Transfer Board (RSIF) ..................................................................................... 26
3.2.9 Temperature Sensor Transfer Board (TMDT) .................................................................... 27
4 PARTS REPLACEMENT .................................................................................................................... 28
4.1 INSTRUMENT ASSEMBLY .............................................................................................................. 28

4.2 LIGHT SOURCE LAMP REPLACEMENT ........................................................................................ 28
4.3 REACTION CUP REPLACEMENT ................................................................................................... 31
4.4 PUMP\VALVE REPLACEMENT ....................................................................................................... 32
4.5 CABLES REPLACEMENT ................................................................................................................ 33
5 MAINTENANCE TO THE INSTRUMENT ........................................................................................... 35
HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 1 of 46


5.1 BASIC MAINTENANCE .................................................................................................................... 35

5.1.1 Daily Maintenance ............................................................................................................. 35
5.1.2 Weekly Maintenance ......................................................................................................... 35
5.1.3 Monthly maintenance......................................................................................................... 35
5.1.4 Biannual maintenance ....................................................................................................... 36
5.2 THE TREATMENT TO BLOCKED SAMPLING NEEDLE ................................................................. 36
5.3 CLEAN THE WASHING STATION.................................................................................................... 37
6 TROUBLE SHOOTING....................................................................................................................... 39
6.1 COMMON TROUBLE SHOOTINGS................................................................................................. 39


1 Instrument Introduction
Principles and Intended use
The instrument Is using the principle of colorimetry, suitable for clinical routine biochemical tests. This device
has no external energy output, no risk of over-temperature, excess radiation output. The matters needing
attention mainly include mechanical motion risk and electric shock risk: when repairing or dissemble the cover
for other reasons, you must cut off the power and shutdown the mainframe.
HTI BioChem FC-200 is easy to operate, has friendly interface to user, It is a good choice for clinical
chemistry laboratories.
HTI BioChem FC-200 system has the following features:
1. Powerful software, five worksheets, each one with 39 samples.
2. 38 reagent positions, position of single and double reagent can be set randomly.
3. Display and print results in different styles, including the storage of test results and info of patients.
4. End point, two point and kinetic method, test with double-wavelength is supported.
5. Calculation by calibration, factor and other method.
6. 8 standard filters: 340nm, 405nm, 450nm, 510nm, 546nm, 578nm, 630nm, 670nm, Other wavelengths
are optional according to user’s requirement.
7. The system will continuously detect and control during the operation, to avoid errors, Error message will
be displayed when detected errors, provide instruction to the user to choose correct buttons and
operations
Main structure of instrument

The outside view of the HTI BioChem FC-200 automatic biochemical analyzer is shown below:


The instrument mainframe is mainly composed of sample/reagent processing system, mixing system,
reaction system, and photoelectric detection system. The structure of each function module is as following:
1.2.1 Sample/reagent disc
The sample and reagent disc are located in a same big plate body, and there are 40 sample positions and 40
reagent positions.
The sample bracket supports the standard test tube, the original blood sampling tube, and the micro sample
cup, the specifications are as follows:
 Standard test tube: Ф12×68.5, Ф12×99, Ф12.7×75, Ф12.7×100, Ф13×75, Ф13×100;
 Original blood sampling tube: Ф12×68.5, Ф12×99, Ф12.7×75, Ф12.7× 100, Ф13×75, Ф13×100;


 Micro sample cup: Ф10 × 37, Ф12 × 37
The reagent disc can hold two sizes of reagent bottles (one big, another small), the volumes are 40ml and 18ml,
and the reagent bottles of different sizes can be used together.
The system will automatically detect the positions of the working reagents. Before each operation, you can
check the position of each reagent should be placed, as well as the total volume of reagent required for this test.
Indications will also be displayed.
The core functional components of the reagent/sample disc cooling system is composed of fan, radiator, Peltier
and other component parts. The cool side of peltier is close to the bottom of the refrigeration cabin but the hot
side should be close to the radiator.

1.2.2 Sampling system
The driver component of the sampling system has two optical couplers, one located at the vertical direction,
another at the horizontal direction, to determine the original mechanical position of the vertical and horizontal
directions precisely. The system also has two stepping motors on the vertical and horizontal directions to control
the accurate movement of the sampling needle at the vertical and horizontal directions, which is driven by belt
and pulley.
The sampling needle with liquid sensor function will contact with the liquid, to aspirate the required volume.
When the reagent or sample is not enough, the instrument will give prompt message automatically. During the
operation of HTI BioChem FC-200, the aspiration cycle of the sampling needle is as follows:
1. The sampling needle enters the bottle of the reagent 1, aspirate reagent with required volume, and eliminate

the return error;
2. The sampling needle moves to the reaction cup and dispense reagent 1;
3. The sampling needle moves to the washing station, and perform washing;
4. The sampling needle moves to the sample cup to aspirate sample with preset volume, and eliminate the
return error;
5. The sampling needle moves to the washing station for cleaning;
6. Transfer the sample and reagent into the Reaction disc wells;
7. The mechanic arm moves back to the washing station, to wash the sampling needle and mixer;
8. A bit of air and 100μL distilled water is aspirated into the flowcell, rinse the flowcell to clean the remaining
samples of last test.
9. The sampling needle aspirate 80μL reaction liquid and a bit of air into the flowcell, rinse the flowcell to prevent
the cross-contamination between samples;
10. Aspirate the remaining reaction liquid into the flowcell for measurement;
11. The mechanic arm moves back to its original position (the washing station); clean the sampling needle and
the sample-aspiration circle starts again.
Generally, when testing with end-point method items and double-reagents items, you can set the stirring time
as 1 ~ 2 seconds.


When pre-heating the reagent, if the required volume of the reagent is more than 250 micro liters, the frozen
reagent should be treated with the pre-heating module, and the temperature is 30 ~ 35 ℃
after pre-heating.
Reagent pre-heating needs an over-temperature protection, and an intelligent temperature control.
Before adding into the reaction cup, the reagent mainly obtains heat from the tube of the middle heating section,
perform the pre-heating by the mixing of high temperature reagent and low temperature reagent.
In order to ensure the accuracy and repeatability of test results, the suggested sample volume should be more
than 4μL.
1.2.3 Sampling washing system
The sampling washing system and the stirring washing system use the same materials, called "Washing station";
use water-spring-type washing method to wash the surface of needle and the stirring rod.
When the operator has performed an illegal operation or errors of procedure occurred, make the instrument
perform wrong actions, the waste liquid in the waste bottle may overflow, it will be drained from the mandatory
drainage holes at the bottom of instrument.
1.2.4 Stirring system

The stirring rod using the structure of cam, it has two limit positions in the washing station and the reaction cup,


this structure is simple and practical, and the risk of movement has also been reduced in a certain degree. Oar-
shaped mixing head can achieve rapid mixing effect through the micro DC motor.
1.2.5 Reaction disc temperature control
 Using air bath, three fans inside, to make internal hot air rotate and circulate up and down;
 Using high quality insulation materials externally will effectively resist the interference to the internal
temperature-controlled room from the external environment.
 using over-temperature protection, the system will cut off the power if the heating air exceeds a certain
temperature to avoid overheating resulting in fires and other accidents;
 Intelligent temperature control system;
1.2.6 Reaction disc
The Reaction disc pot body is the same with the reagent/specimen disc body with external insulation, but the
external insulation is mainly to ensure that the Reaction disc internal temperature control easy to be
implemented, because if the effect of insulation is not good, the reaction disc outside will be featured with fast
heat exchange and it is not good to the implementation of constant temperature.


Reaction cup replacement entrance
External insulation
Light source part of the
optical assembly
1.2.7 Reaction Cup
There are 81 reaction positions. Use high-quality plastic semi-permanent reaction cup, with jointly linked
structure, each row has 9 cups; the optical path is 5 ± 0.02mm;
1.2.8 Optical System
Standard configuration: 340, 405, 450, 510, 546, 578, 630, 670 nm filters, total of 8 wavelengths. Interference
filter, front spectrum, has a separate dark current detection channel. Using 2,000 hours long-life tungsten
halogen lamp, 12 V, 20W.


Photodiode component
Optical block
Light source component Filter wheel component
1.2.9 Heat emission and dust-proof of the whole machine
 The light source uses a direct thermal fan, without any separate air path, use the negative pressure to
aspirate air naturally;
 The reagent refrigeration air path is separate;
 The whole machine heat emission adopts downdraught system; the whole machine can aspirate air from
front and side air.

1.2.10 Display
HTI BioChem FC-200 uses color LCD screen to display WINDOWS software, with friendly user interface.
Operator can operate the software step by step according to the related instructions.
Technical parameters
System Random optional, multi-channel, multi-parameters
Test Type Colorimetric method, immuneoturbidimetric method, etc.
Weight 140kg (desktop)
Power AC220V ,50-60Hz
Fuse 250 V/10 A
Analysis methods End-point, two-point, kinetics method, support double reagents
and double-wavelengths
Filter 8 optical filters (340,405,450,510,546,578,630,670 nm)


Temperature Control Ambient temperature 25 ℃, 30℃, 37℃
Reagent positions 40
Reagent volume Less than 500uL
Specimen positions 40
Specimen volume 3 ~ 45uL
Light source Halogen lamp 12V, 20W
Linear range 0-3.5 Abs±1%
Sensitivity 0.0001Abs
Work environment 15-30 ℃, relative hum idity 18

-85%
Test Method Factor method, single-point standard, multi-point standard


2 Instrument Installation and calibration
Instrument unsealing
Unpack the package of the instrument and remove the materials for transport. Save the packing box and the
packaging materials well, for your convenience in the future of re-packaging the instrument.
1) Take out the instrument from the box.
2) Take away the packaging materials, and take out the instrument from the plastic packaging bag.
3) Check the items in the box and confirm that the following items are within the box:
 HTI BioChem FC-200 mainframe
 Operator’s Manual
 Packing List
 Warranty certificate
 Accessories: power cord, printer, print cable, LCD monitor, mouse, keyboard, RS-232 serial cable, spare
fuses, etc. (see packing list).
Note: If the items are inconsistent with the packing list or have any defects, please contact with the
supplier.
Installation Environment
HTI BioChem FC-200 auto chemistry analyzer must be installed by authorized professionals. The instrument
must be installed in a clean dust-free operation room, to avoid shock, humidity, strong magnetic fields and direct
light. The working environment is of 15 ℃~ 30℃, the relative hum idity is 35~
-condensing).
The power supply must be 220V/50 Hz, and should be well grounded. If the laboratory voltage change is greater
than +10%, it is recommended to install an external regulator above 1500-watt. As this machine is a full
computer-controlled precision instrument, it is proposed to install an UPS above 2000W (uninterruptible back-
up power supply).
The back-face of the instrument should be 0.5 m to the wall at least, in order to ensure the heat dissipation
requirements.
If it is a desktop machine, the water bucket should be placed at the right lower adjacent to the analyzer, pay
attention to ensure that the waste liquid pipe should always be downward, to avoid the situation such as upward


wrap (it can be resolved by adjusting the tube length).
In order to ensure the normal operation of the instrument, it is prohibit to placing the instrument in the following
places:
 A place of large temperature difference
 A place of extreme environments in temperature, altitude, and air pressure
 A place of heavy dusts
 A place of vibrated table, and large tilt angle
 A place close to the magnetic field of electromagnetic devices
Note:
●The AC power supply must be well grounded (zero ground voltage <5V).
●The AC power must be stable and it is prohibited to share power supply with the appliances of high
power. Recommend to fit with a large 2000W UPS Power Supply.
●When unplugging the power cord, please seize the plug itself, rather than the power cord.
●If the instrument is found to have smoke, smell or abnormal sounds, immediately turn off the power,
and contact with the HTI service center immediately.
Instrument connections
1. Interface of the whole machine
RS-232: Standard RS-232 Interface
Ground Interface
2. Electrical interface and switch
AC Power Input
Fuse T10AL 250V
Reagent refrigerated position


CPU
3. The external bucket connection interfaces
If the color mark is still remained, you can connect according to the color mark, if the color identification is
missed, then connect in line with the following figure to ensure proper connection.


3 Functional Description
HTI BioChem FC-200 function box






Function description of each boards

3.2.1 Main board (MCON)
The main board is machine's central control unit, including the mid-position machine, and five hypostasis
machine modules;
The mid-position machine is responsible for the channel scheduling of the five control modules;
Module 1 is for the movement control of the reagent disc motor and the filter wheel motor;
Module 2 is for the movement control of the reaction disc motor and the auto-rinsing motor;
Module 3 is for the control of the sampling needle up and down movement motor, the sampling needle rotating
motor, and the syringe motor;
Module 4 is for the control of the stirring rod up and down movement motor and the stirring rod rotation DC

motor;
Module 5 is an independent board, it is a temperature control module, which can independently complete the
temperature control for the reaction disc constant temperature heating, sampling needle pre-heating, auto-
rinsing pre-heating, and the reagent disc cooling;
The interfaces are defined as follows:
(1) Testing components:
OC1: Reagent/specimen disc initial position sensor
OC2: Reagent/code disc of specimen disc sensor
OC3: Filter wheel initial position sensor
OC4: Reaction disc initial sensor
OC5: Code disc of reaction disc sensor
OC6: Auto-rinsing upper and lower sensor
OC7: Sampling needle up and down position sensor
OC8: Sampling needle rotation initial position sensor
OC9: Syringe initial position sensor
OC10: Stirring rod initial position sensor
(2) Control components (motor part):
JM1: Reagent/specimen disc motor
JM2: Filter Wheel Motor
JM3: Reaction disc motor
JM4：Auto-rinsing motor
JM5: Sampling needle up and down movement motor
JM6: Sampling needle rotation motor
JM7: Syringe motor
JM8: Stirring rod up and down movement motor


DM: Stirring rod rotation DC motor
(3) Control components (pump valve part):
V1: Inner wall cleaning valve
V2: Auto-rinsing liquid feeding valve A, 1-2-step
V3: Auto-rinsing liquid feeding valve B, 3-6-step
V4: Auto-rinsing liquid feeding valve C, 7-8-step
P1: Sampling needle inner wall scavenging pump
P2: Sampling needle outer wall scavenging pump
P3: Auto-rinsing liquid feeding valve A, 1-2-step
P4: Auto-rinsing liquid feeding pump B, 3-6-step
P5: Auto-rinsing liquid feeding pump C, 7-8-step
P6: Auto-rinsing liquid dispensing pump A, 1-2-step
P7: Auto-rinsing liquid dispensing pump B, 3-7-step
P8: Auto-rinsing liquid dispensing pump C, 8-step
(4) Control components (buzzer):
BUZZER1: Wash liquid and waste liquid alarm;
BUZZER2: Sampling needle movement fault alarm.
(5) Some interfaces:
J1: The interface to serial port transfer board, communicate with computer;
U15: The interface to the temperature control board, and communicate with it;
JP4: The interface to the liquid feeding and dispensing alarming board;
J2: The interface to the optic-electronic amplification board;
HP: Power Interface.


Picture of main board (PCB No.001)
3.2.2 Temperature Control Board (TCON)
Temperature Control Board performs the whole machine temperature control, it can complete the temperature
control for the reaction disc constant temperature heating, sampling needle pre-heating, auto-rinsing pre-
heating, and the reagent disc cooling independently;
The control parameters will be loaded through the serial port by the main board, and the temperature data will
be inquired by the main board.
(1) Testing components:
T1: Reaction disc temperature sensor;
H_HEAT1: Sampling needle pre-heating temperature sensor;
H_HEAT3: Auto-rinsing pre-heating temperature sensor;
H_COLD: Reagent disc cooling temperature sensor;


(2) Control components (motor part):
J1: Reaction disc heating membrane;
J3: Sampling needle heating rod;
J5: Auto-rinsing heating rod;
PX1: Cooling Peltier 1;
(3) Some interfaces:
PWCN: heating control power interface;
PW3: refrigeration control power interface;
PW1: AC power input interface;
HP: Power Interface.
Picture of temperature control board (PCB No.002)


3.2.3 Photoelectric conversion board (IVAB)
Photoelectric conversion board is to convert the photodiode current signal into voltage signal, and perform the
first-class amplification, A1 = 1.5
The interface is defined as follows:
J1: the signal output interface.
The specific circuit diagram is as follows:
J1
3.2.4 Photoelectric amplification board (ADCB)
The photoelectric amplification board is for the second stage amplification of the photoelectric signal, the
magnification is adjusted by the digital potentiometer, the specific regulation should follow the corresponding
settings in the engineering debugging software, the magnification A2 = 1 + (R Number/1k);
Input the former two stage amplified signal into the AD conversion chip for analog-digital conversion, the control
signal is from the main board, and the output signal will return back to the main board;
Total photoelectric signal amplification, A = A1 * A2.
Interfaces are defined as follows:
J1: The signal interface to the main board.
J2: The interface to the photoelectric conversion board.


Picture of photoelectric amplification board (PCB No.004)
J2
J1
3.2.5 Optic-coupler fixing board (OCBD)
The machine has 10 correlation-type infrared photoelectric sensors, commonly known as optic-coupler;
The 10 correlation-type infrared photoelectric sensors will be fixed on board, connected to the main board by
3pin cable, supporting the main board for collecting the position signals of various fixed optic-couplers.
J1: The interface to the main board optic-coupler input end.
Picture of optic-coupler fixing board (PCB No.005)


J1
3.2.6 Feeding and dispensing liquid alarm board (WSIF)
This board will connect to the alarm sensors of the wash bottles and the waste bottle, the connector is designed
as audio interface, connect to the main board through the 10pin flat cable, for the alarm circuit signal processing;
It is designed with two-way cleaning liquid bottle alarm inputs, two-way waste liquid bottle alarm inputs, which
should be installed according to the actual situation.
JP1: Feeding and dispensing liquid alarm line interface;
WASH1: wash liquid 1 alarm line input;
WASH2: wash liquid 2 alarm line input;
WASTE1: Waste liquid 1 alarm line input;
WASTE2: Waste liquid 2 alarm line input;
Picture of feeding and dispensing liquid alarm board (PCB No.006):
JP1
WASH WASH2 WASTE1 WASTE2


3.2.7 Liquid level detection board (LDCB)
The liquid level detection board is to perform the liquid level detection when the sampling needle is aspirating
the reagent or specimen, and the test principle is capacitance. When moving the sampling needle up and down,
from up and down movement to contact with the liquid surface, the capacitance of the sampling needle will
change, the adjusted voltage signal will change, refers to the liquid surface information;
The crash-proof signal detection of the sampling needle up and down movement will be completed by the
combination of an optic-coupler and a retaining piece;
The liquid level detection signal and the up and down crash-proof signal will be connected to the main board
through 5pin flexible cable, for signal judgment.
J1: The interface connected to the sampling needle.
JP1: The interface to the main board.
Picture of liquid level detection board (PCB No.007):
TP1
J1 TP2 VR1 VR2 VR3 JP1
3.2.8 Serial Port Transfer Board (RSIF)
It is the Serial Port transfer board of the whole instrument, one end will be connected to the main board serial
converter with 3 pin cable, and the other end will be connected to the computer serial port through an external
serial cable.
J1: The interface to the main board.


RS232: The interface to the computer.
Picture of serial port transfer board (PCB No.008):
RS232
J1
3.2.9 Temperature Sensor Transfer Board (TMDT)
J1: Temperature control board interface.
Picture of temperature Sensor transfer board (PCB No.009):
J1


4 Parts Replacement
Instrument assembly
HTI BioChem FC-200 is a high- precision instrument, if the instrument has trouble and can not be resolved with
the method in this manual, it should be repaired by professionals; If it is confirmed that the internal parts of the
instrument have some troubles which should be replaced for repairing, please follow the below steps to
dismantle the instrument:
1. Turn off the instrument power switch (unplug the power cord to prevent from turning on the power switch
accidently).
2. Remove the eight screws which fix the rear-board of the machine with a screwdriver and remove the rear
board, pay attention to protect the board, avoid falling damage.
3. After removing the rear-board, you can see the instrument's internal hardware boards, pump valves, the
board sockets of the cable at a glance, so it is convenient for electrical maintenance.
Light source lamp replacement
The lamp light source will gradually age to make energy attenuation when used for a long time, if the system has
given out alarm message, or the total working time is estimated to be over 1000 hours, need to replace the light
source lamp.
1. Turn off the power to make the lamp and lamp box cool for 30 minutes.

Note:
Must wait more than 30 minutes after shutdown, even after 30 minutes, you must be careful to touch the shell
body of the light source lamp, because the hot lamp and lamp box will cause skin burns.
2. Unscrew the light source cover at the worktable.
3. Unscrew the three cross screws on the top of the light source body shell.
4. Wait until the light source shell will not burn your skin because of high temperature, pinch the light source
cable connectors with hand and hold up the shell;
5. Remove the light source components, replace new light source lamp, connect the light source line well, and
then put back the supporting base and lock the three cross screws unscrewed before.
Note:
When assembling or disassembling the light source, even if the lamp has no heat, please do not touch
the translucent lamp surface with your hands, because this may change the characteristics of the light
source due to grease, dirt and other reasons, if inadvertently make the translucent surface stained with
grease, dirt, you can wipe it with a clean cotton swab dipped with absolute ethanol.


Light source shell fixing
screw
Light source connection
cable
Light source component fixing screw
6. The light source lamp has been positioned by screws, no need for fine-tuning, but because of the differences
of various lamps, you need to adjust the received light energy, it is recommended to replace all the reaction
cups here, in order to avoid the large differences between the old and new cups. Method 1: turn on the
instrument, enter into the "HTI BioChem FC-200 auto chemistry analyzer management software" → "Status" →
"Reaction disc", click on “Change Cuvette”" button, and then you can replace it based on prompt, (method 2: or
from the " HTI BioChem FC-200 auto chemistry analyzer management software," → "Maintenance" → "Blank"
and then click on "Change Cuvette" button, but note that this method requires a user authorization; Method 3:
"Engineering debugging software" → "Blank" → "Change Cuvette".
7. Users usually operate only with method 1 and 2, after correct replacement of the reaction cup, from the " HTI
BioChem FC-200 auto chemistry analyzer management software" → "Maintenance" → "Blank" and then click
on “Check Blank” button, record the proximate light intensity AD value of each wavelength, and then enter into
"Maintenance"→ “Configure”, adjust the magnitude ratio of each wavelength, make the light intensity AD value
of each wavelength to be about 55000, but for the wavelengths over 510nm, its AD value could be about 57000
(if it can not successfully auto-configure the magnitude ratio, you must configure it manually, the AD value is
proportional to the configured value), after completing the configuration, please perform the blank check once
more, please observe whether it is appropriate or not, you may need to repeat several times before it meets the
requirements.


8. After confirming that there is no problem, you can tighten the light source cover of the worktable.
Note: When adjusting the location of the light source lamp, do not let the light directly shine your eyes, because
the strong light may hurt your eyes.
Reaction cup replacement
1) Open the reaction cup replacement entrance on the worktable.
2) The method to replace the reaction cup is the same with the steps mentioned in section 4.2. Method 1: turn
on the instrument, enter into the "HTI BioChem FC-200 auto chemistry analyzer management software" →
"Status" → "Reaction disc", click on “Change Cuvette”" button, and then you can replace it based on prompt,
(method 2: or from the " HTI BioChem FC-200 auto chemistry analyzer management software," →
"Maintenance" → "Blank" and then click on "Change Cuvette" button, but note that this method requires a user
authorization; Method 3: "Engineering debugging software" → "Blank" → "Change Cuvette".
3) If the instrument is with auto-rinsing system, when replacing the reaction cup, carefully unscrew the reaction
cup fixing screw; do not let it slide inside. If it has been slid inside, the proposed operation is to shutdown firstly,

and rotates the reaction disc in place by hand or takes the falling screw out with tweezers or magnetic
screwdriver.
4) If the instrument is not with auto-rinsing system, when replacing the reaction cup, pay attention to take it out
vertically to prevent the reaction cup orientation column breaking accidently; when placing the new reaction
cup, please put it vertically, after it has been positioned well, press the cup joint slightly, to check whether it
is flat or not;
5) Because that the reaction cups may have differences in different batches, it is proposed that you should
observe the Cuvette blank after replacing the reaction cup; from " HTI BioChem FC-200 auto chemistry
analyzer management software," → "Maintenance" → "Blank" and then click on “Check Blank” button,
observe the proximate light intensity AD value of each wavelength, to determine whether need to adjust the
magnitude ratio;
6) Close the reaction cup replacement entrance cover-plate on the worktable.
The reaction cup replacement
entrance cover-plate
Pump\valve Replacement
Firstly, disassemble all the pump\valve components requiring for replacement, and then replace the specific
pump\valves.


Sampling needle inner and
outer wall washing pump valve

Auto-rinsing pump valve
fixing component
fixing component
Note: Pay attention to the bonding wire on the pump valve, so as not to break; be careful of liquid spilling when
you unplugging the pipes.
Cables replacement


When replacing the cables, please pay attention to the labels on them, do not replace them with the cable
of incorrect labels, if you really have no correct label on the cables, you can write the correct label on a piece of
paper and cover it onto the original label, then twist it with transparent tape.
When plug and unplug the cables, please do not shake around.


5 Maintenance to the instrument
HTI BioChem FC-200 is a precise clinical analyzer, in order to maintaining the instrument in a good condition;
you must do the routine maintenance work well. The maintenance of HTI BioChem FC-200 is very simple, but
it must be performed carefully.
Basic Maintenance
5.1.1 Daily Maintenance
Keep the instrument working environment clean.
The cleaning of the instrument surface must be performed with some neutral detergents and damp cloth.
LCD display must be cleaned with a soft cloth.
Check the sampling needle to make sure that it is in good condition, no impairment, and no dirt; wipe it with
some neutral detergents and cloth.
Check the stirring rod to make sure that it is in good condition, no impairment, and no dirt; wipe it with some
neutral detergents and cloth.
Check the deionized-water bucket/waste-liquid bucket/cleaning-liquid bucket to determine whether the
connection of the pipeline is normal, and whether need to add water or drain.
Check whether the syringe is leaky.
Check whether the paper inside the printer is adequate.
5.1.2 Weekly Maintenance
Rinse the sampling needle
Rinse the stirring rod
Clean the instrument panel
Clean the reagent/specimen disc cavity
Rinse the deionized-water bottle
Rinse the waste bottle
5.1.3 Monthly maintenance
Rinse the inner and outer wall of the sampling needle
Rinse the washing station of the cleaning needle and the stirring rod


Rinse the syringe
Clean the dust screen of the outside shell fan
Prime the pipeline with cleanser
5.1.4 Biannual maintenance
Clean the ball spline shaft and the ball bearing of the sampling needle component (with grease lubrication)
Clean the guide rail of the stirring rod component (with grease lubrication)
Rinse the washing station: Add several milliliter of diluted bleach (5:1000), clean the inner surface of the
visible part of the washing station by a swab;
Recommend to replace the lamp
The treatment to blocked sampling needle
1. Turn off the instrument power, and open the rear end plate of the instrument.
2. Pull out the connector piece between the valve and the transferring jointer, as shown in the following picture.
3. Prepare a syringe of 10ml and aspirate the distilled water, install a cuvette head on the syringe and insert it
into the pipeline just pulled out, push the syringe to inject the water into the washing station (the needle must
be on the top of the washing station at this time), repeat the above step for more times until the water flow
from sampling needle of cuvette head smoothly.


Pull out the pipe here

Inner wall pump
Outer wall pump, connect to
washing tank
Clean the washing station
In order to avoid the breeding of bacteria, we recommend users to clean the washing station (the washing
stations of the sampling needle and the stirring rod) regularly. Infuse the diluted bleach liquid of 5:1000 into the
washing station and keep it for 15 to 20 minutes, then wipe it with a medical swab, after wiping, rinse with plenty
of distilled water to ensure that there is no cotton residue in the washing station.


Stirring rod washing
station
Sampling needle
washing station


6 Trouble Shooting
Common Trouble Shootings
Trouble Solutions
Analyzer can not start up - Check whether the power plug is loose
- Check the fuse
- Check the voltage
The lamp has no light - Check whether the power is normal before replacing the lamp
- If the computer and instrument can start normally, just no light,
replace the lamp please.
Operating software can not be - Shutdown and wait for about 10 seconds, then restart the
initialized machine
- Check whether the printer plug or the computer printer

Printer can not start up
connection is loose
- Check the ON / OFF button of the printer
- Check the fuse
- Check whether the printer plug or the computer printer

Printer can not print
connection is properly connected
No liquid gushes from the

- Check whether the pump of the washing station is working
washing station
- Check whether the related pipeline of the washing station is
properly connected
- Check whether there is water in the deionized water bottle


Bubble and spattering liquid gush - If the liquid-path is not used for a long-term, this happens during
from the washing station the early prime period.
- Check whether the related pipeline of the washing station is
properly connected or broken
- Check whether there is water in the deionized water bottle
- The pipette may have been aging, should be replaced
Absorbency/blank AD value is
- Check whether the light source is emitting light normally
displayed incorrectly
- Try to execute the reading under another wavelength to provide
the estimation
- Check whether the parts of the light-path is loose or installed
error
Blank value is too high - Rinsing the reaction cup
- Check the cleanser
- Check the lamp
- Check whether the sampling needle is blocked
- Check the blood sample to determine whether the hemolysis
occurs or the height of the serum liquid is less than 3mm.
Poor reproducibility of the results -Some reaction cup may be contaminated, replace it
- Check the aspiration status of the sampling needle
- Check the parameter settings of the reagent
- Replace the lamp and adjust the magnitude ratio
- The connection air-tightness of the syringe T-way pipeline is not
good
- The sample or the reagent is contaminated or deteriorated
- Check whether the volume of the tested sample is adequate


- Check whether the margin of the reagent is adequate
The aspiration volume of the

- Check whether the sampling needle is blocked
sampling needle is not a constant
- Check the connection air-tightness of the syringe T-way
pipeline
Quality control is not within the - Check the period of validity of the quality control liquid, and
target value verify that the quality control liquid is not contaminated
- Check the item parameter settings, and determine whether the
parameter needs to be modified
- Re-test with another method
- Check the incubation temperature of the Reaction disc, retest
with a new reagent or the quality control material
There are a lot of air bubbles in - Check the connection air-tightness of the syringe T-way
the reaction cup pipeline
- Check whether the sample volume of 450 micro-liter is ok
(determine the stroke)
- Check whether the work of the syringe motor is normal (judge
the speed)
There are bulbs in syringe - Confirm that the piston is tight and no-leak.
- Clean the syringe with Tween 20 solution (2 drops / liter distilled
water)
- Replace the inner O-ring of the syringe
- Check the pipeline connected with the syringe


- Check the connection air-tightness of the syringe T-way

Sample is contaminated
pipeline
- Carry out the appropriate cleaning or replace the sampling
needle
- Clean the washing station
- Confirm that there is no residual liquid in the sampling needle
- Confirm whether the deionized is stored for a long time or
contaminated
- The sample cup is dirty, need to replace a new cup or a one-off
cup
- Check the temperature settings, appropriately adjust the

Incubation chamber is not warm
parameter to ensure it keep at 37 ± 0.2 ℃
Liquid leaks from the analyzer - Check whether the draining pipeline of the waste liquid is
below inserted into the waste liquid bucket
- The waste liquid bottle is filled out
- The pipeline of the washing station is blocked
● If there is any instrument failure, please contact with the dealer in time to obtain technical support!
【HTI BioChem FC-200: Calculation item settings】
As for the calculation item, recommend to use the following codes to fill in its item name and item code.
1. GLB (Globin) = TP (Total protein)-ALB (Albumin)
ALB / GLB the ratio of albumin and globin
2. TBIL (Total bilirubin)-DBIL (Direct bilirubin) = IBIL (Indirect bilirubin)
3. AST / ALT the ratio of aspartate transaminase and alanine transaminase
4. LDH / AST the ratio of lactate dehydrogenase/aspartate transaminase
5. GGT / AST the ratio of GGT / aspartate transaminase
6. ApoA / ApoB the ratio of apolipoprotein A/apolipoprotein B


7. LDL (Low Density Lipoprotein) = CHO (Total Cholesterol)-HDL (High Density Lipoprotein)-TRIG
(Triglyceride)/2.2
Note:
This manual may contain other proprietary notices and copyright information, and the related
provisions must be observed. The information in this manual may contain some technical
inaccuracies or typographical errors.
This manual may be changed or updated without notice. HTI can also improve and/or change the
described products and/or processes without notice at any time.

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High Technology, Inc.

109 Production Road, Walpole MA 02081

Automated Chemistry Analyzer

Document Number : SM-E-FC240

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

1.1 PRINCIPLES AND INTENDED USE .................................................................................................. 3

2 INSTRUMENT INSTALLATION AND CALIBRATION ....................................................................... 12

2.1 INSTRUMENT UNSEALING............................................................................................................. 12

3 FUNCTIONAL DESCRIPTION ........................................................................................................... 15

3.1 HTI BIOCHEM FC-200 FUNCTION BOX ......................................................................................... 15

4 PARTS REPLACEMENT .................................................................................................................... 28

4.1 INSTRUMENT ASSEMBLY .............................................................................................................. 28

5 MAINTENANCE TO THE INSTRUMENT ........................................................................................... 35

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 1 of 46

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

5.1 BASIC MAINTENANCE .................................................................................................................... 35

6.1 COMMON TROUBLE SHOOTINGS................................................................................................. 39

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 2 of 43

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

HTI BioChem FC-200 system has the following features:

1. Powerful software, five worksheets, each one with 39 samples.

5. Calculation by calibration, factor and other method.

are optional according to user’s requirement.

Main structure of instrument

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 3 of 46

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

1.2.1 Sample/reagent disc

cup, the specifications are as follows:

 Standard test tube: Ф12×68.5, Ф12×99, Ф12.7×75, Ф12.7×100, Ф13×75, Ф13×100;

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 4 of 43

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

 Micro sample cup: Ф10 × 37, Ф12 × 37

and the reagent bottles of different sizes can be used together.

Indications will also be displayed.

side should be close to the radiator.

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

the return error;

5. The sampling needle moves to the washing station for cleaning;

samples of last test.

the cross-contamination between samples;

the sample-aspiration circle starts again.

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 6 of 43

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

Reagent pre-heating needs an over-temperature protection, and an intelligent temperature control.

1.2.3 Sampling washing system

drainage holes at the bottom of instrument.

1.2.4 Stirring system

HIGH TECHNOLOGY, INC. PROPRIETARY AND CONFIDENTIAL Page 7 of 43

High Technology, Inc. Revision Level Rev.A

Service Manual: HTI BioChem FC-200

1.2.5 Reaction disc temperature control

temperature-controlled room from the external environment.

temperature to avoid overheating resulting in fires and other accidents;

 Intelligent temperature control system;