Saint Mary’s University

SCHOOL OF HEALTH and NATURALSCIENCES

Medical Laboratory Science Department
Bayombong, Nueva Vizcaya
GROUP NO. 2 IMMUNOHHEMATOLOGY LAB 5148
Becudo, Lindsay BMLS 3A2
Cairo, Kyan Marie
Francisco, Kenneth Ralph
Juan, Kimberly
Yumol, Chloe Nicolae
BROAD SPECTRUM COMPATIBILITY TEST
PRINCIPLE: Broad-spectrum compatibility test in blood banking is to determine the compatibility
between the donor's blood and the recipient's blood before a blood transfusion takes place. This
test is commonly known as a crossmatch. It involves mixing a sample of the donor's blood with a
sample of the recipient's blood and observing the reaction between the two. The purpose is to
identify any potential incompatibilities that could lead to an adverse immune response in the
recipient if incompatible blood is transfused.
PROCEDURE:

A. Collection of Specimen

1. Collect 2 same blood type samples for the

Donor and Patient specimen using Syringe
Venipuncture Procedure

B. Protein/ Albumin/ Room temperature

phase (immediate saline spin)

1. Label 4 wasserman tubes as M, MA, N and

NA

2.
In Tube M, contains 2 drops of patient's serum
and 1 drop of 2-5% RCS of donor's cells.
In Tube MA, contains 2 drops of patient's
serum, 1 drop of 2-5% RCS of donor's cells and
2 drops of BSA.
In Tube N, contains 2 drops of donor's serum
and 1 drop of 2-5% RCS of patient's cells.
 Saint Mary’s University
SCHOOL OF HEALTH and NATURALSCIENCES
Medical Laboratory Science Department
Bayombong, Nueva Vizcaya

In Tube NA, contains 2 drops of donor's serum,

1 drop of 2-5% RCS of patient's cells and 2
drops of BSA.

3. After mixing, centrifuge the tubes for 15

seconds at 3400 rpm.

4. Gently dislodge the cell button and observe

agglutination and if no agglutination MA and
NA tubess will proceed to THERMO PHASE.

C. Thermo/ Incubation Phase (37⁰C)

1. Incubate MA and NA tubes for 30 minutes at

37⁰C

2. Centrifuge for 15 seconds at 3400 rpm

 Saint Mary’s University
SCHOOL OF HEALTH and NATURALSCIENCES
Medical Laboratory Science Department
Bayombong, Nueva Vizcaya

3. Gently dislodge and if no agglutination is

observed in tubes MA or NA proceed to AHG
phase.

Based from the results, there is no

agglutination or hemolysis observed.
Interpretation:
Therefore the Donor and Patient is
COMPATIBLE for Blood transfusion.