Saint Mary’s University

SCHOOL OF HEALTH and NATURAL SCIENCES

Medical Laboratory Science Department
Bayombong, Nueva Vizcaya

GROUP NO. 2 IMMUNOHHEMATOLOGY LAB 5148

Becudo, Lindsay BMLS 3A2

Cairo, Kyan Marie

Francisco, Kenneth Ralph

Juan, Kimberly

Yumol, Chloe Nicolae

DETERMINATION OF SPECIFIC AGGLUTINATION TITRE OF COMMERCIAL ANTISERA

Serial dilution is a series of simple dilution in which the concentration of a substance

decreases by the same quantity in each successive step and is used in the determination of antibody
titre.

The sample was initially determined, labelled

type A and type B. Blood collection was done
via venipuncture.

Multiple test tubes were utilized, containing

NSS. Each was labelled from the dilution ratio
1:1 through 1:152. A red cell suspension was
prepared.

BMLS 3A-2
 Saint Mary’s University
SCHOOL OF HEALTH and NATURAL SCIENCES
Medical Laboratory Science Department
Bayombong, Nueva Vizcaya

The Anti-A, Anti-B and 5% cell suspension were

mixed in each tube and were consecutively
transferred one tube to the other with each
achieving 0.2 mL volume of liquid.

After centrifugation, the following appearances

of the mixtures were obtained.

Results:

The titre of the blood type A falls unto the ratio

1:4, meaning that one part serum to 4 parts
saline solution (dilutant) results in no
antibodies remaining detectable in the next
tubes.

The titre of the blood type B falls unto the ratio

1:16, meaning that one part serum to 16 parts
saline solution (dilutant) results in no
antibodies remaining detectable in the next
tubes.

BMLS 3A-2