Research Question: 01/30123

what
is an important part

of
the transporter needed for

Efflux
ofneurotransmitter

Drug Design

I
BASIC Scaffold (NOSP) AU

Transporter
* EAACI CRAT)

TopOlOgY
*

EAATS
* (1-3)

transporters
·
neuron (10%) -

o r glial
astrocyte cells (90%) VERYimpORTANT glutamate

a fully through cell membrane

of animals
a lot
Natbinding
toops 523 amino adds ↓

si
SS3side view amino and chainone
Hairpin loop 1
highly conserved
area across many
7 Transmembrane domains 2 Harpin loop structures
species (frogs, rats,

humans)

Trimer.3 conformational

folding
* of
change
a .d. for a structure -
multiple How
↓
we know?

BLAST
↳ depends on environ. (sugar, fat, salt)
HPLI * Very conserved
- likely
I not binding site

EAAC

saits
1
a llows
Not glutamate to enter
NaTK
k allows glutamate to leave
+

concentration
dependent

· after stroke Nate itlevels are outof

whack results in conformational
which change in EAACI.

Changes in EAACI result

in glutamate efflux which is cytotoxic cell death

in of
replication
Bacteria or19
reSISTANCE
⑲ plasmid
Of
antbIOHC
DNA
*

double
*
stranded
GAAC
replicable
*
plasmid in bacteria