STAPHYLOCOCCUS LISTERIA ERYSIPELOTHRIX STREPTOCOCCUS RHODOCOCCUS

Gram-positive cocci
Gram positive coccobacilli Gram positive, small rods (smooth form), or Gram-positive spherical/ovoid cocci Gram positive
✓ S Spherical cells arranged in irregular filaments (rough form)
✓ Clu clusters (G.staphyle “bunch of grapes arranged in pairs and long chains
✓ Kokokkos “berry”), but may appear in coccus or bacilli
✓
Pairs short chains
Cellular Lack spores and flagella non-spore forming non-spore forming Non-spore forming Non-sporeforming
characteristics No capsules in vitro acapsular acapsular Can form capsules and slime layers Capsule
stable in the environment non-acid fast Non-acid fast fastidious bacteria Variably acid-fast
Non- motile Motile, at 250C with tumbling non-motile Non-motile non-motile
movement
Facultative anaerobes facultative anaerobes Facultative anaerobe but grows best in candle Facultative anaerobes Aerobic, grows on non-enriched
*anaerobic – S. anaerobius and S.saccharolyticus jar or 10% CO2 media
Growth on BAP at 37C Colonies on BAP at 37C Growth on BAP at 37oC
grow on ordinary medium grow on non-enriched media over a Grows well on ordinary media.
wide temperature range 4oC to 45oC Grows on non-enriched media over a wide Most parasitic forms are fastidious and
Cultural ❖ Non-selective medium: BAP temperature range require enriched media.
characteristics
❖ Differential medium: Two colony forms initial growth requires microaerophilic
Mannitol Salt Agar - contains 7.5% NaCl 1. R form (rough)- chronic syndrome conditions
(filaments)
❖ Selective medium: Baird Parker
Medium 2. S form (smooth)- acute syndrome
(rods)
• Colonies are large, convex, opaque small, translucent with narrow zone of colonies are small and transparent to semi- small, shiny, round, smooth, mucoid,
incomplete hemolysis opaque larger mucoid and salmon pink in
• porcelain white or yellowish orange/ golden color with age
yellow colonies Reaction in TSI: A/A , H2S formed along stab line Small non-pigmented colonies
in TSI agar
Colonies of some isolates growing on blood agar
produce two concentric zones of hemolysis Small hemolytic colonies on blood narrow zone of incomplete hemolysis most pathogenic Streptococcus produce a non-hemolytic
agar wide zone of complete (beta) hemolysis
• Hemolysis in sheep or ox blood agar around the colony
- alpha, beta, gamma, delta

Traditionally classified based on ability to

coagulate plasma
- CPS: Mostly hemolytic
(Produce either a zone of complete hemolysis or a
double zone of hemolysis;
CNS: usually non-hemolytic

Salt tolerant – halophile ❖ Tolerates wide Grows over a wide temperature range Pleomorphic
temp.and pH ranges
• Commensals on the skin of animals and humans pleomorphic
• Mucous mem.of the URT and lower genital tract ❖ tolerate pH values
• Transients in the digestive tract between 5.5 and 9.6

❖ Aesculin hydrolysed
almost 60 recognized species known genus Erysipelothrix consists of six species and Currently there are 103 recognized species
28 serotypes. and 9 subspecies within the genus
Species
Catalase + Catalase + Catalase – Catalase – Catalase +
)catalase negative – S. anaerobius and (Rhodococcus equi)
S.saccharolyticus)
 Oxidase – Oxidase – Oxidase – Oxidase – oxidase (cytochrome) negative

(Rhodococcus equi)

Coagulase + (e.g., S.aureus) Coagulase +

Biochemical
characteristics S.xylosus and S.sciuri – most common coagulase
negative
fermentative metabolism
fermentative metabolism Variable fermentative reactions do not ferment carbohydrates
– all spp. of streptococcus are
homofermentative organisms, producing
lactic acid from glucose

Indole – Indole –
• Cause pyogenic infections of the body
surfaces, respiratory tract, urinary tract
or mammary gland
• Abscesses are a common sequelae

NOTE: Wala po akong reviewer ng mga diseases from Staph to Rhodo ☹