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  • Vibrio Aeromonas Plesiomonas and Campylobacter

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    1. Vibrio, Aeromonas, Plesiomonas, and Campylobacter are gram-negative bacteria that can cause intestinal diseases. Vibrio includes pathogens like V. cholerae. 2. These bacteria can be isolated from stool, blood, and wounds using selective media like TCBS agar. Identification involves Gram staining, oxidase and biochemical tests. 3. Aeromonas species are oxidase and indole positive and colonies are β-hemolytic. Plesiomonas is oxidase positive. Campylobacter forms small, gray colonies on selective media in microaerophilic conditions.

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    1. Vibrio, Aeromonas, Plesiomonas, and Campylobacter are gram-negative bacteria that can cause intestinal diseases. Vibrio includes pathogens like V. cholerae. 2. These bacteria can be isolated from stool, blood, and wounds using selective media like TCBS agar. Identification involves Gram staining, oxidase and biochemical tests. 3. Aeromonas species are oxidase and indole positive and colonies are β-hemolytic. Plesiomonas is oxidase positive. Campylobacter forms small, gray colonies on selective media in microaerophilic conditions.

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    15 views4 pages

    Vibrio Aeromonas Plesiomonas and Campylobacter

    Uploaded by

    Captain Gummy
    1. Vibrio, Aeromonas, Plesiomonas, and Campylobacter are gram-negative bacteria that can cause intestinal diseases. Vibrio includes pathogens like V. cholerae. 2. These bacteria can be isolated from stool, blood, and wounds using selective media like TCBS agar. Identification involves Gram staining, oxidase and biochemical tests. 3. Aeromonas species are oxidase and indole positive and colonies are β-hemolytic. Plesiomonas is oxidase positive. Campylobacter forms small, gray colonies on selective media in microaerophilic conditions.

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    of 4

    VIBRIO

    • curved gram-negative rods that may occasionally join together,


    Vibrio, Aeromonas, Plesiomonas, forming S shapes
    • cause intestinal diseases
    and Campylobacter • V. cholerae
    • V. parahaemolyticus
    • V. alginolyticus
    • V. vulnificus

    • Specimens submitted for culture: feces and rectal swabs


    • Observe the colonial
    • blood and wound cultures
    morphology of V.
    • BA, CHOC, and MAC
    parahaemolyticus, V.
    • MAC: non lactose fermenters, with the exception of V. vulnificus: lactose fermenter.
    alginolyticus, and
    • oxidase positive
    Aeromonas spp. on blood
    • sucrose positive.
    agar, MacConkey, and TCBS.
    • Thiosulfate citrate bile sucrose (TCBS) agar is used to selectively isolate Vibrio spp.
    on stool specimens along with an alkaline peptone broth supplemented with 3%
    NaCl.
    Species Growth in NaCl Vogues- Lysine Lactose Sucrose
    Proskauer
    0% 6%

    Vibrio − + + + − +
    alginolyticus
    Vibrio cholerae + +/− +/− + − +

    Vibrio − + − + − −
    parahaemolyticus
    Vibrio vulnificus − +/- − + + −
    • Prepare a smear from the
    colony and Gram stain.
    Describe the microscopic
    morphology and record
    results.
    • Perform catalase (+) and
    oxidase (+) tests.
    • Inoculate the following
    biochemical test media—TSI,
    V P, lysine, lactose, sucrose,
    nutrient broth with NaCl (0%,
    3%, 6%, 8%)—and incubate
    all cultures for 18 to 24 hours
    at 35 C.

    • 1. Observe the colonial


    AEROMONAS & PLESIOMONAS morphology of A. hydrophila on blood
    agar and MacConkey.
    • Aeromonas spp. cells are straight • Plesiomonas spp. • 2. Perform the following
    rather than curved • blood and spinal fluid, feces procedures:
    • A. hydrophila • oxidase positive • Prepare a smear and Gram stain.
    • oxidase and indole positive, and Describe the microscopic morphology
    the colonies are usually β- and record results.
    hemolytic on blood agar • Perform an oxidase test.
    • feces and rectal swabs, blood and • Inoculate the following slant and/or
    wound cultures Biochemical Mini ID system—TSI and
    Characteristics Vibrio spp. Aeromonas spp. API 20E—and incubate all cultures at
    35 C for 18 to 24 hours.
    Oxidase + + • 3. Read and record all results.

    Sensitivity to 0129 vibriostatic + −


    compound
    Polar flagella + −
    CAMPYLOBACTER

    • C. jejuni and C. coli

    • curved or S-shaped gram-negative rods

    • oxidase positive, catalase positive, motile, and microaerophilic, and some species may be
    selectively isolated at 42 C

    • They also require a selective medium for isolation from stool specimens, made of
    Brucella agar, sheep blood, and a combination of antimicrobials: vancomycin, bacitracin,
    cephalothin, amphotericin B, trimethoprim, and polymyxin B.

    • Skirrow’s medium and CAMPY-CVA are examples of this type of medium.

    Campylobacter

    Prepare a smear and Gram stain. Observe the microscopic morphology.


    Observe and examine the colonial morphology of C. jejuni on curved
    CAMPY agar medium after incubation in a microaerophilic non–spore-forming, gram-negative rods
    environment (Campy bag, Campy jar, or Anoxomat). long spirals or S- or seagull-wing shapes
    small, gray, flat, non-hemolytic, mucoid colonies at 24 and 48 hours
    Perform a
    susceptibility test
    using nalidixic acid
    and cephalothin
    antimicrobial disks

    Perform an
    oxidase,
    catalase, and
    Hippurate
    hydrolysis
    test
    .

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