8

Biotechnology Activity Sheet

Quarter 1 - MELC 8
Week 7
Sterilization and Disinfection

REGION VI – WESTERN VISAYAS

Biotechnology
Activity Sheet No. 7 - Sterilization and Disinfection
First Edition, 2021

Published in the Philippines

By the Department of Education
Region 6 -- Western Visayas

Republic Act 8293, section 176 states that: No copyright shall subsist
in any work of the Government of the Philippines. However, prior approval of
the government agency or office wherein the work is created shall be necessary
for exploitation of such work for profit. Such agency or office may, among other
things, impose as a condition the payment of royalties.

This Learning Activity Sheet is developed by DepEd Region 6 – Western

Visayas.

ALL RIGHTS RESERVED. No part of this learning resource may be

reproduced or transmitted in any form or by any means electronic or
mechanical without written permission from the DepEd Regional Office 6 –
Western Visayas.
Development Team of Biotechnology Activity Sheet

Writer: Ruby Ann Dahay- Angelino

Editor: Daisy L. Lopez
Carmel Joy P. Aujero
Layout Artists: Jerry R. Bagiuos
Schools Division Quality Assurance Team:
Ma. Cristina G. Caballero
Reynaldo A. Sumaylo
Lovenia D. Pansaon
Daisy L. Lopez
Carmel Joy P. Aujero
Division of Silay City Management Team:
Novelyn M. Vilchez
Luz U Banson
Federico P. Pillon Jr.
Daisy L. Lopez
Carmel Joy P. Aujero
Regional Management Team:
Ramir B. Uytico
Pedro T. Escobar, Jr
Elena P. Gonzaga
Donald T. Genine
Rovel R. Salcedo
Moonyeen C. Rivera
Anita S. Gubalane
Minda L. Soldevilla
Daisy L. Lopez
Joseph M. Pagalaran

Int
Introductory Message:
Welcome to Biotechnology!

This Learning Activity Sheet is a product of the collaborative efforts of

the Schools Division of Silay City and DepEd Regional Office VI- Western
Visayas through the Curriculum Learning Management Division (CLMD). This
is developed to guide the learning facilitators (teachers, parents and
responsible adults) in helping the learners to meet the standards set by the K
to 12 Basic Education Curriculum.

This Learning Activity Sheet is self-directed instructional materials

aimed to guide the learners in accomplishing activities at their own pace and
time using the contextualized resources in the community. This will also assist
the learners in acquiring the lifelong learning skills, knowledge and attitudes
for productivity and employment.

For learning facilitator:

The Biotechnology Activity Sheet will help you facilitate the teaching-
learning activities specified in each Most Essential Learning Competency
(MELC) with minimal or no face-to-face encounter between you and learner.
This will be made available to the learners with the reference/links to ease the
independent learning.

For the learner:

The Biotechnology Activity Sheet is developed to helped you continue

learning even if you are not in school. This learning material provides with
meaningful and engaging activities for independent learning. Being an active
learner, carefully read and understand the instructions then perform the
activities and answer the assessmentsts. This will be returned to your
facilitator on the agreed schedule.
Name of Learner: _________________________________________________________
Grade and Section: _______________________________ Date: _________________

BIOTECHNOLOGY ACTIVITY SHEET No. 7

Sterilization and Disinfection

I. Learning Competency
Cite an example of effective sterilization and disinfection technique and
its importance in our daily life.

II. Background Information for Learners

Keeping your living space and common surfaces clean is important for
your health. But this is not the same thing as disinfecting or sterilizing.
Disinfecting and sterilizing are both types of decontamination, a process that
makes something safe to touch. The purpose is to kill enough germs so the
risk of infection is extremely low (Cherney, 2021).

Sometimes the terms sterilize, sanitize and disinfect are used

interchangeably. However, these terms don’t mean the same. These are all
antimicrobial processes that targets to kill germs but it is important to know
what mode of decontamination is appropriate to use based on a specific
situation.

This learning activity will help you understand the differences and
similarities of sterilization and disinfection.

III. Activity

Activity 1: Let’s Arrange using Definition

Directions: Arrange the scrambled letters below to get the correct terms. Use the given
definition as your clue.

1. SRIILTEAZNIOT- a process that removes all microorganisms and typically used

by professionals in settings such as hospitals.

ANSWER: _________________________________________________________________

2. NNFDIISEOICT- removes harmful organisms from objects and usually done by

applying chemical sprays or wipes.

ANSWER: _________________________________________________________________

3. TAMITIONNACONDE- reduction or removal of chemical agents.

ANSWER: _________________________________________________________________

4. ZEITSANI- reduce or eliminate bacteria on the surfaces of objects.

ANSWER: _________________________________________________________________
Activity 2: Sterilization vs Disinfection

Directions: Read and study the article below. Compare and contrast sterilization and
disinfection using Venn diagram.

Disinfection

The process of disinfecting removes harmful organisms from objects. This is usually
done by applying chemical sprays or wipes. Disinfectants can kill most viruses and
fungi, with the majority of commercial products also marketed as effective against the
COVID-19 virus when used as directed.

Sterilization

Sterilization, on the other hand, is a process typically used by professionals in

settings such as hospitals. While disinfecting gets rid of most germs, sterilization
removes all microorganisms — including those that are not harmful. Sterilization is
common in medical facilities, but it may also be helpful for businesses and schools that
want to get rid of germs in entire rooms.

Methods of sterilization may include:

• pressurized steam (autoclaving)

• hydrogen peroxide gas
• ethylene oxide (EtO) gas
• ionizing radiation (typically used for medical equipment)
• dry heat cabinets (for medical instruments)
• infrared radiation
• advanced filtration

Regular cleaning is an important way to keep you and your family healthy. But to
effectively kill harmful microorganisms such as COVID-19, you will also need to
disinfect common surfaces. Disinfecting kills most harmful bacteria, viruses, and fungi.
Sterilizing may be more appropriate for larger commercial settings and medical facilities
because it’s guaranteed to get rid of all microorganisms. Proper sterilizing methods are
always done by professionals. But you can disinfect surfaces on your own at home and
in your workplace. Carefully follow all product directions, and avoid mixing chemicals
or using them in nonventilated areas.
 STERILIZATION VS DISINFECTION

Sterilization Disinfection

Based on the article read, explain the importance of sterilization and

disinfection in our lives.
___________________________________________________________________________
___________________________________________________________________________

IV. Reflection
Complete the statement below.
After doing the learning activity I learned that
___________________________________________________________________________
___________________________________________________________________________

V. Answer Key

that is to kill germs that may harm one’s health.

disinfection are two different concepts however targets the same goal and
After doing the learning activity sheet I learned that sterilization and
IV. Reflection

Activity 2
4. SANITIZE
3. DECONTAMINATION
2. DISINFECTION
1. STERILIZATION
Activity 1
VI. Links and/or Other References

Google. “cdc.gov.” Accessed July 28, 2021

https://www.cdc.gov/infectioncontrol/guidelines/disinfection/introduction.html

Google. “healthline.com” Accessed July 28, 2021

https://www.healthline.com/health/disinfect-vs-sterilize#takeaway

Google. “ncbi.nlm.gov” Accessed July 28, 2021

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158362/

Google. “sciecnedirect.com” Accessed July 28, 2021

https://www.sciencedirect.com/topics/chemistry/sterilization-and-disinfection

Google. “fas.org” Accessed August 7, 2021

https://www.fas.org.mmch/decontamination

Google. “merriam-webster.com” Accessed August 7, 2021

https://www.merriam-webster.com