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1. Megakaryoblast
Megakaryocyte
Earliest recognizable form in EM
40-120 um in diameter (due to continue as
15-50 um diameter
endo mitosis)
Makes up less <1% of all nucleated cells
Nucleus:
in the BM
I. Multilobed
Cytoplasm:
II. Nucleoli not visible
I. Basophilic
III. Deeply and variably condensed
II. With blunt protrusions
chromatin
Nucleus:
Cytoplasm:
I. Single and centrally located or
I. Azurophilic and granular
multiple with round and oval
Alpha granules(+)
nuclei
Dense granules(+)
II. Multiple distinct nucleoli
DMS(+)
III. Fine and delicate chromatin
N/C ratio:1:4
strands
N/C ratio:3:1 Note:
Note: It has no endo mitosis.
Largest cell in the bone marrow
The maturation sequence of Megakaryoblast
takes about 5 days.
Platelets are produces directly to the 3.Metamegakaryocyte
Megakaryocyte cytoplasm.
As the megakaryocyte matures, clusters of Disintegrated cell surrounded by platelets
granules aggregate to form your platelets. Note:
First maturation, which is the megakaryoblast,
the start of endo mitosis. It sheds 2000-4000 of platelets
4.Platelets/Thrombocyte
2.Promegakaryocyte
Cytoplasmic fragments of the megakaryocytes
20-80 um diameter 1-4 um diameter
Development of demarcating membranes Light blue to purple
system(DMS) Very granular
Nucleus: I. ·Alpha granules
I. ·Intensely indented or lobulated II. ·Dense granules
II. ·Chromatin becomes coarse Life span:8-11 days
Cytoplasm:
I. ·Abundant Note:
II. ·Basophilic The circulating platelets is 2/3 of the total
III. ·Granular platelets that is 70 to 80 percent.
Alpha granules(+) Stored platelets in spleen are 1/3 of the total
Dense granules(+) platelets which is 20 to 30 percent.
DMS(+)
N/C ratio:1:2
PLATELET STRUCTURE SOURCE SUBSTANCE
ALPHA HMWK ( High Molecular Weight Kininogen)
A. Peripheral zone GRANULES Fibrinogen
Factor V
a. Glycocalyx (outer surface) Factor VII:vWF
Platelet factor 4
Incorporate with gp la. Ib. Ic. lib. III. IV and V
Thrombospondin
Provides surface for adhesion of coagulation Platelet-derived growth factor
factors 1,5,11,12 and 13 Beta-thromboglobulin
Riasminogen
b. Plasma membrane Alpha-2 antiplasmin
C1 esterase inhibitor
Physical and chemical barrier
DENSE ADP
Contains the sodium-potassium ATPase ionic GRANULES Calcium
pump Serotonin
Contains phospholipid constituents Sa Henry’s Book dinagdag yung
ATP tsaka Thyroid phosphate
c. Submembranous area
D. Membrane system