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S-MEDB413LA MEB34 1st Sem ( 2022-2023 )

Eukaryotic Gene Expression Problem Set

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Question 1
Post transcriptional modification to the 3'-end of eukaryotic mRNAs.What is added to the 3'-end of many eukaryotic mRNAs after transcription?
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Response: a poly A tail

Question 2
Why do pre-mRNAs get smaller during RNA processing?The primary RNA transcript of the chicken ovalbumin gene is 7700 nucleotides long, but the
mature mRNA that is translated on the ribosome is 1872 nucleotides long. This size difference occurs primarily as a result of:

Response: splicing

Question 3
Information transfer to the cytoplasm.Which of the following molecules functions to transfer information from the nucleus to the cytoplasm?

Response: mRNA

Question 4
Self-catalytic RNAsRNAs that catalyze biological reactions, such as self-splicing introns, are known as:

Response: ribozymes

Question 5
Features of hnRNA.Which of the following features would you NOT expect to find in heterogeneous nuclear RNA (hnRNA)?

Response: polycistronic coding

Question 6
Hybridization of mRNA with the DNA coding strand.mRNA will form hybrids only with the coding strand of DNA because:

Response: RNA:DNA hybridization follows the base-pairing rules

Question 7
Features of nuclear RNA processing.Which of the following is not part of RNA processing in eukaryotes?

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Response: reverse transcription

Question 8
What is the part of a gene that will encode a part of the final mature RNA produced by that gene after introns have been removed by RNA splicing. It
also refers to both the DNA sequence within a gene and to the corresponding sequence in RNA transcripts.

Response: introns

Question 9
DNA-DNA renaturation and DNA-RNA hybridizationWhich statement is NOT true about nucleic acid hybridization?

Response: a polysaccharide can hybridize with a DNA strand

Question 10
Regulating mRNA synthesis.Which of the following is NOT involved in regulating the synthesis of RNA in the eukaryotic nucleus?

Response: spliceosomes that stimulate synthesis of intron-containing hnRNAs

Question 11
Features of eukaryotic mRNAs.Which of the following is NOT a feature of eukaryotic gene expression?

Response: RNA synthesis and protein synthesis are coupled as in prokaryotes

Question 12
Protein coding sequencesThe regions of DNA in a eukaryotic gene that encode a polypeptide product are called:

Response: exons

Question 13
PromotersPromoters for eukaryotic mRNA synthesis:

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Response: all of these

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