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    mankarravindra61
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    1) The objective was to study pollen germination on a stigma through a permanent slide. 2) Materials like fresh flowers, microscope, slides, cover slips, sucrose, boric acid, potassium nitrate, and magnesium sulfate were used. 3) Pollen grains were placed on a nutrient medium on a slide, observed under a microscope, and were found to germinate, with the tube cell forming a pollen tube that contains the tube nucleus and male gametes.

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    1) The objective was to study pollen germination on a stigma through a permanent slide. 2) Materials like fresh flowers, microscope, slides, cover slips, sucrose, boric acid, potassium nitrate, and magnesium sulfate were used. 3) Pollen grains were placed on a nutrient medium on a slide, observed under a microscope, and were found to germinate, with the tube cell forming a pollen tube that contains the tube nucleus and male gametes.

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    © All Rights Reserved
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    7 views1 page

    Expt No. 7

    Uploaded by

    mankarravindra61
    1) The objective was to study pollen germination on a stigma through a permanent slide. 2) Materials like fresh flowers, microscope, slides, cover slips, sucrose, boric acid, potassium nitrate, and magnesium sulfate were used. 3) Pollen grains were placed on a nutrient medium on a slide, observed under a microscope, and were found to germinate, with the tube cell forming a pollen tube that contains the tube nucleus and male gametes.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 1

    OBJECTIVE

    To study
    the polen germination on stigma through apermanent slide.
    MATERIALS REQUIRED:
    Fresh flowers, microscope, slide, cover slips, sucrose, boric acid, potassium nitrate, magnesium sulphate,
    beakers, etc.
    PROCEDURE
    1. Prepare anutrient medium by mixing 10 gm sucrose, 10 mg boric acid, 30 mg magnesium sulphate and 20
    mg potassium nitrate in 100 ml of water.
    2. Take a few drops ofthis nutrient medium on a slide and dusta few pollen grains from the stamen on it. Leave
    them undisturbed for about 10 minutes.
    3. Observe the slide under a microscope.
    OBSERVATIONSAND CONCLUSION
    The pollen grains germinate in a nutrient medium. The tube cell enlarges to come out of thepollen grain through
    one of the germ pores and forms a pollen tube. The tube nucleus reaches the tip ofthis pollen tube. The generative cell
    also passes into it and divides into two male gametes.

    Intine
    Exine
    Germ pores.

    Pollen
    Tube nucleus - tube

    Male gametes

    Fig. 11.1: Germination of pollen grains

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