St.

John’s Public School

— CHENNAI - 600 100

CHEMISTRY
PROJECT REPORT

2023-2024

PAPER CHROMATOGRAPHY

submitted to

SENIOR SECONDARY CERTIFICATE

EXAMINATION

Done By

THARUN.S.P
XII-E
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project

2023 -2024

ALL INDIA SENIOR SECONDARY SCHOOL EXAMINATION

EXAMNITATION

ST. JOHN’S PUBLIC SCHOOL, CHENNAI - 600100

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ACKNOWLEDGEMENT

I would like to express my special thanks of gratitude to my teacher

Mrs.Swapna madam for her guidance, and support throughout the project. I

completed the project successfully with her motivation and her extended

support for us.

As well as I would also like to thank our Correspondent Dr. R. Kishore

Kumar, and our Principal Mrs. Shanthi Samuel who allowed me to work on

this project, which in turn helped me in doing a lot of research and analysis on

the chosen topic.

Finally, I would also like to thank my parents and friends who supported me

in completing this project within the limited time frame.

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TABLE OF CONTENTS
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INTRODUCTION
Paper chromatography is a technique which is used to separate low-molecular-
mass compounds based on their distribution between stationary phase
and mobile phase. Due to its low cost and availability of various protocols for
the separation of compounds, paper chromatography is considered a powerful
analytical tool. The method consists of applying the test solution or sample as a
spot near one corner of a sheet of filter paper
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PRINCIPAL OF CHROMATOGRAPHY

The principle involved can be partition chromatography or adsorption

chromatography. Partition chromatography because the substances are
partitioned or distributed between liquid phases. The two phases are water held
in pores of the filter paper and the other phase is a mobile phase which passes
through the paper. When the mobile phase moves, the separation of the
mixture takes place. The compounds in the mixture separate themselves based
on the differences in their affinity towards stationary and mobile phase solvents
under the capillary action of pores in the paper. Adsorption chromatography
between solid and liquid phases, wherein the solid surface of the paper is the
stationary phase and the liquid phase is the mobile phase.

PREPARATION OF CHROMATOGRAPHY
PAPER
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WORKING OF CHROMATOGRAPHHIC PAPER

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TYPES OF CHROMATOGRAPHY
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DIFFERENT TYPES OF DEVELOPMENT

TECHNIQUES
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Rf VALUES
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FACTORS AFFECTING Rf VALUES

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SOURCES OF ERROR
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SEPERATING COLOURED PIGMENTS

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SAMPLE SPOT DETECTION AND

DEVELOPMENT OF COLOUR
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APPLICATIONS OF PAPER
CHROMATOGRAPHY
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BIBLIOGRAPHY
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