Professional Documents
Culture Documents
M.S. Patil
Assistant Professor of Horticulture
College of Agriculture, Pune-411005 (Maharashtra)
A.R. Karale
Ex. Associate Dean and Principal
College of Horticulture, Pune-411005 (Maharashtra)
C.D. Badgujar
Assistant Professor of Horticulture
NARP, Ganeshkhind, Pune-412007 (Maharashtra)
J.D. Adiga
Professor of Fruit Science
College of Horticulture, UHS, Campus GKVK, Bengaluru-560065 (Karnataka)
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Preface
A secret of life is not just enjoyment but
getting education through experience
— Swami Vivekananda
Most of the Agricultural Universities, Institutes, Organizations and Government selection
agencies have made competitive examinations as the basis for choosing candidates. The
questions asked in these examinations are of objective type, large in number and of
varied nature. It requires quick answering within a specified period of time to secure
high merit and assured success.
The aspirants have to search a lot of printed and e-material to cover each topic of syllabus
thoroughly. This is so because there is hardly any high quality book available on
horticulture that covers the entire syllabus of this subject for competitive examinations.
Keeping this in mind, a sincere effort has been made to write this book named ‘Essence
of Horticulture’ covering various branches of horticulture. This book contains quite an
adequate material for seven Divisions of Horticulture, namely, Fruit science, Vegetable
science, Floriculture and Landscape Gardening, Plantation crops, Spices and
Condiments, Medicinal and Aromatic Plants and Post Harvest Technology.
The book has been written keeping in view the pattern of different horticulture/
agriculture competitive entrance examinations viz. JRF, SRF, ARS, ASRB, NET & Ph.D.
(Horticulture). We hope that this book will be useful for U.G, P.G. and Ph.D. horticulture
students, teachers and horticulture people across the country.
Special appreciation is expressed to the many contributors who have helped and
contributed in providing the questions from different divisions and for their helpful
suggestions during the preparation of the manuscript. We are thankful to Dr. V.K.
Garande, Mr. C.Y. Dhumal, Dr. Manoj Nazir, Dr. P. Pavan Kumar from various disciplines
of Horticulture for their help and valuable suggestion. We also thank to New India
Publishing Agency to bring the excellent format of this book.
We have attempted to bring this information as much as possible but all the information
available on the subject cannot be presented in a text of this size. This presentation is our
compromise.
We will feel more satisfied if this book can serve the purpose of students to succeed in
various competitive examinations.
All suggestions for future improvement for this book will be greatly solicited.
Pune M.S.Patil
A.R. Karale
C.D. Badgujar
J.D. Adiga
Contents
Horticulture word derived from Latin word: Hortus meaning ‘Garden’ and
cultura means ‘cultivation’.
Father of horticulture: Thomas Andrew Knight, John Lindley, Liberty Hyde
Bailey.
1st artificial hybridization in fruit crop in world: T. A. Knight.
Father of vegetables: L. H. Bailey.
1st book in horticulture: Fruit growing in India: W.B. Hayes (1945) & related
to litchi.
Export growth of fresh fruits and vegetables in term of value is 14% and of
processed fruits and vegetables is 16.27%.
Contribution of horticulture sector to agriculture GDP (2013-14): 29%.
India is largest producer of Coconut, Cashew, Tea and Spices.
India ranks first in productivity of grapes, banana, cassava, peas, and papaya.
India’s rank in fruit production: 2nd (1st Brazil).
India’s rank in vegetable production: 2nd (1st China).
India is largest producer of 1. Mango 2. Banana 3. Sapota.
India is largest producer of 1. Onion 2. Potato.
Per capita consumption of fruit (recommended): 184 g/person/ year.
Per capita consumption of vegetable (recommended): 200 g/person/year.
Per capita consumption of fruit (available): 70-80 g/day/person.
Per capita consumption of vegetable (available): 220 g/day/person.
Horticultural Society of India (HSI) was established in 1942.
1st president of Horticultural Society of India (HSI): Dr. G.S. Cheema.
1st Indian Horticulture Congress was held in 2004.
Rainbow revolution: Over all horticulture production.
2 Essence of Horticulture
Fruit science
Name of institute Location
Central Arid Zone Research Institute (CAZRI) Jodhpur, Rajasthan
Central Institute for Sub-tropical Horticulture (CISTH) Lucknow, Uttar Pradesh
earlier Central Mango Research Station
Central Institute for Temperate Horticulture (CITH) Srinagar, Jammu and Kashmir
Central Institute for Arid Horticulture (CIAH) Bikaner, Rajasthan
Central Institute of Horticulture (CIH) Mediziphema, Nagaland
Central Research Institute for Chikoo (CRIC) Muzzaffarpur, Bihar
National Research Centre for Banana (NRCB) Trichy, Tamil Nadu
Central Citrus Research Institute (earlier NRCC) Nagpur, Maharashtra
National Research Centre for Grapes (NRCG) Pune, Maharashtra
National Research Centre for Litchi (NRCL) Muzzaffarpur, Bihar
National Research Centre for Makhana (NRCM) Darbhanga, Bihar
National Research Centre on Pomegranate (NRCP) Solapur, Maharashtra
Vegetable science
Name of institute Location
Central Potato Research Institute (CPRI) Shimla, Himachal Pradesh
Central Tuber Crops Research Institute (CTCRI) Tiruvanantapuram, Kerala
Directorate of Onion and Garlic (DOG) Pune, Maharashtra
Indian Institute of Vegetable Research (IIVR) Varanasi, Uttar Pradesh
National Horticultural Research and Nashik, Maharashtra
Development Foundation (NHRDF)
Directorate of Mushroom Research (DMR) Solan, Himachal Pradesh
Floriculture
Name of institute Location
Directorate of Floricultural Research (DFR) Pune, Maharashtra
National Research Centre for Orchids (NRCO) Gangtok, Sikkim
National Botanical Research Institute (NBRI) Lucknow, Uttar Pradesh
Institute of Himalayan Bio resource Technology (IHBT) Palampur, Himachal Pradesh
National Institute of Post Harvest Technology (NIPHT) Pune, Maharashtra
Orchid Research and Development Centre (ORDC) Bhalokpong, Arunachal
Pradesh
6 Essence of Horticulture
Plantation crops
Name of institute Location
Central Coffee Research Institute (CCRI) Chikmagalur, Karnataka
Central Plantation Crops Research Institute (CPCRI) Kozhikode, Kerala
Directorate of Cashew Research (DCR) Puttur, Karnataka
Directorate of Cashew nut and Cocoa Development (DCCD) Kochi, Kerala
Directorate of Arecanut and Spices Development (DASD) Kozhikode, Kerala
Indian Institute of Oil Palm Research (earlier DOPR) West Godavari, Andhra
Pradesh
United Planters’ Association of Southern India (UPASI) Coonoor, Tamil Nadu
UPASI Tea Research Foundation (UPASI TRF) Coimbatore, Tamil Nadu
Central Arecanut and Cocoa Marketing and Processing Mangalore, Karnataka
Co-operative Limited (CAMPCO)
Cashew Export Promotion Council of India Ernakulum, Kerala
Central Coir Research Institute Kerala
Coconut Development Board (CDB) Kochi, Kerala
Coffee Board Bengaluru, Karnataka
Rubber Board Kottayam, Kerala
Tea Board Kolkata
Spices
Name of institute Location
Indian Institute of Spice Research (IISR) Kozhikode, Kerala
Indian Cardamom Research Institute (ICRI) Myladumpara, Idukki, Kerala
National Research Centre for Seed Spices (NRCSS) Ajmer, Rajasthan
Spice Board Kochi, Kerala
General Horticulture 7
Other institutes
Name of institute Location
Central Plant Protection Training Institute (CPPTI) Hyderabad, Andhra Pradesh
National Research Centre for Integrated Pest New Delhi
Management (NRCIPM)
National Institute of Hydrology (NIH) Roorkee, Uttarakhand
National Bureau of Plant Genetic Resources (NBPGR) New Delhi
National Bureau of Agriculturally Bengaluru, Karnataka
Important Insects (NBAII)
National Bureau of Agriculturally Important Kushmaur, Uttar Pradesh
Micro-organisms (NBAIM)
National Research Centre on DNA Fingerprinting NBPGR, New Delhi
CSIR Laboratory Palampur, Himachal Pradesh
National Centre for Integrated Pest New Delhi
Management (NCIPM)
Central Plant Protection Training Institute (CPPTI) Hyderabad
8 Essence of Horticulture
Role
a. Polar translocation (apex to downward movement).
b. Apical dominance
c. Promotes or inhibit abscission of leaves, flower, fruits.
d. Stimulate cell elongation.
e. Promotes or inhibits growth of adventitious roots.
2. Gibberellin
Discovered by Yabuta and Sumiki
Precursor: Terpenoids (Mevalonic acid).
Fungus: Gibberella fujikuroi.
Foolish disease of rice.
Synthesized in young leaves.
Anti-gibberellin action: Paclobutrazol, Flurprimidol, Ancymidol, AMO 1618.
12 Essence of Horticulture
Role
a. Stimulate cell elongation, cell division (internode elongation).
b. Promotes seed germination i.e. more ratio of GA to ABA.
c. Tolerance to chilling
d. Induction of flowering in long day plant
e. Prevention of genetic and physiological dwarfism
f. Breaking dormancy (seed, tuber ad shoot)
g. Stimulates bolting in rosette plants.
3. Cytokinin
Richmond Lang effect.
Skoog and Miller coined auxin-cytokinin hypothesis
Precursor: 5’-AMP (Isopentenyl group)
Natural cytokinin: Zeatin
Synthetic cytokinin: Kinetin, Benzyl adenine
Synthesized in root tips
Role
a. Initiation of cell division
b. Delaying senescence (Richmond long effect)
c. Cell enlargement
d. Differentiation of cells (interact with auxin)
e. Induces flowering in short day plants
4. Abscissic acid
Natural plant hormone.
Stress hormone.
Excess shade causes production of ABA.
Strongest senescence stimulator.
Precursor: Sesquiterpenoid pathway (Mevalonic acid)
Synthesized at terminal bud.
General Horticulture 13
Role
a. Bud dormancy
b. Stimulates closure of stomata
c. Induction and maintenance of dormancy
d. Induction of desiccation tolerance
e. Disease resistance
f. Protecting cells from dehydration
5. Ethylene
Gaseous hormone
Precursor: Methionine.
Discovered by Gane.
Role
a. Stimulates shoot, root growth and differentiation.
b. Enhance latex flow in rubber
c. Stimulates leaf and fruit abscission (phytogerontological hormone)
d. Induction of flowering in pineapple.
e. Initiation of ripening in citrus, banana, tomato
f. Stimulates flower and leaf senescence
5. NUTRITIVE VALUE
Term ‘Nutrification’ is coined by Lachance.
Most fruits and vegetables are not rich source of Vitamin B.
World’s most nutritive fruit: Avocado.
Cobalt is a component of Vitamin B12.
Vitamin that is not found in fruits or vegetables: Vit B12.
The major mineral present in fruits and vegetables: Ca.
Vitamin C: Thermosensitive & antioxidant vitamin.
Vitamin K1: Inhibit banana ripening.
Folic acid 1st found in spinach leaves.
Sulphur deficiency symptoms 1st appear on young leaves.
General Horticulture 15
16 Essence of Horticulture
General Horticulture 17
6. MINERAL NUTRITION
English word “Fertilizer” was derived from Latin word “Fertil”.
Grey revolution: Promotion of manures and fertilizers.
Average all India use of NPK (2015) is: 8.2 : 3.2 : 1.
Essential nutrients: 17.
Criteria of essentiality of nutrients: Arnon and Stout (1954).
Nicholas (1961) proposed the term ‘Functional or Metabolism nutrients’.
The concept of leaf sampling for nutrient sampling is given by Lutherd and
Smith.
Estimated extent of salt affected soils in India: 17.42 Mha.
Net arable land: 120 Mha.
Loamy soil: Maximum microbial activity.
Nutrient mobility concept: Bray.
1st Agricultural chemist of ICAR: J.W. Leather.
1st micronutrient discovered: Fe.
Element found in meristematic tissue: Potassium.
India ranks 2nd in fertilizer consumption.
Most important source of cadmium (Cd) pollution: Phosphate fertilizers.
India imports ‘K’ fertilizers mainly from Germany and France.
Zn and Fe are compatible with each other.
Fertilizers that is not produced in India: MOP.
Basic nutrients: C, H, O.
Macro nutrients: N, P, K, Ca, Mg, S (>100 µg/g dry matter).
18 Essence of Horticulture
Micro nutrients: Cl, Fe, B, Mn, Zn, Cu, Mo (<100 µg/g dry matter).
Best time of application of fertilizers: June and July.
Fertigation: Application of fertilizers through irrigation.
Herbigation: Application of herbicides through irrigation.
Maximum fertilizer use efficiency: Fertigation.
Soil sterilant: Copper sulphate.
Micronutrients most widely deficient in Indian soils Zn and Fe.
Denitrification occurs in waterlogged soils.
Dry matter yield is increased with an application of nitrogenous fertilizers.
The range of deficiency and toxicity is narrow in Mo micronutrient.
Basic structure of plant: C, H, O.
Plants absorb N in nitrate form.
Toxic substance present in urea is Biuret.
Biuret % in urea: 1.5%.
Nitrogen form in urea: Amide & organic.
Term C: N ratio was coined by Karaus and Kraybill in 1919.
C: N ratio concept was given by Klebs (1913).
Low C: N ratio: Promotes vegetative growth.
High C: N ratio: Promotes flowering.
Deficiency symptoms
1. Old leaves- N, P, K, Mg, Mo.
2. New leaves- S, Fe, Mn, Cu.
3. Old and new leaves- Zn.
4. Terminal bud- Cu, B.
base
Pomeg- Leaf Bud differentiation in 8th leaf from apex 50
ranate April for February crop
Sapota Leaf September 10th leaf from apex 30
Fertilizers
1st fertilizer used: Calcium nitrate.
Preservatives used to decrease nitrogen losses in FYM and cattle urine:
Gypsum and super phosphate.
The word compost is derived from the Latin word “COMPONERE” to
mean put together.
Optimum C: N ratio of the composting material is 20:1 to 30:1.
A typical compost earthworm is Eisenia foetida.
The process of composting was first initiated in England during First World
War (1914 -1918).
The Agricultural Development Company (ADCO) process of composting
was patented at Rothamsted Experimental Station, England.
Activated compost process was developed by Fowler and Ridge in 1922 at
Indian Institute of Science, Bengaluru.
22 Essence of Horticulture
a. Inorganic
The 1st ammonia plant was established in 1913 by Fritz Haber and Carp
Bosch at Oppau, Germany for Badische, Anilin and Soda-Fabric A.G (BASF).
The process is called Haber–Bosch process.
Ammonium sulphate: Oldest synthetic fertilizer.
Calcium ammonium nitrate (CAN): Hygroscopic, neutral and explosive
fertilizer.
F. Wholer (German chemist), 1 st prepared urea in 1828 (Commercial
production started in 1922).
Single super phosphate is the oldest artificially produced fertilizer and its
manufacture dates back to 1842, when Lawes J. B prepared for the 1st time
in England by treating rock phosphate with H2SO4.
High performance of rock phosphate to supply P to crop plants is expected
in acidic soil.
The Latin/ German word for the element with symbol K is “Kalium”. It is
derived from the Arab word ‘el-kali’ means ash.
Potassium content of earth crust is 2.40 % whereas the content of phoshorus
is only 0.11%.
All potash fertilizers viz., KCl and K2SO4 used in India is imported.
Recently Geological Survey of India has reported the occurrence of K-
deposits in Leh (J & K) and Bikaner (Rajasthan).
General Horticulture 23
b. Organic
Concentrated: Bone meal, horn meal, blood meal, meat meal, fish meal,
Guano, Oil cakes.
Edible oil cake: Ground nut, Gingelly cakes, etc.
Non-edible cake: Castor cake, Neem cake etc.
Best concentrated manure: Bone meal.
Neem tree is regarded as a “village dispensary”.
24 Essence of Horticulture
d. Organic farming
Austria: Leading country in world.
Leading state in India: Madhya Pradesh.
Organic state: Sikkim, Uttaranchal and Tamil Nadu.
General Horticulture 25
Methods of farming
Biodynamic farming: Aims to treat the farm as a living system.
Rishi Krishi: Practiced in Madhya Pradesh.
Natueco farming: Based on the principle of ecosystem networking of nature.
Regulatory mechanisms
National Programme in Organic Production (NPOP, Ministry of commerce).
NPOP launched in 2001 under FTDR act.
NSOP: National Standards of Organic Production.
Foreign Trade Development and Regulation Act (FTDR) is responsible for
export requirement.
APEDA: Regulation of organic production and export under the brand name
‘India Organic’.
Apex body of organic certification: National Accreditation Body (NAB).
Indian Organic Certification (IOC) process granted at world level during
2004.
National Centre for Organic Farming (NCOF) is located at Ghaziabad, UP
(Ministry of Agriculture).
International Federation of Organic Agriculture Movements (IFOAM),
conceptual support for a globally unified certification database.
IFOAM (1972) is located at Bonn, Germany.
7. NURSERY MANAGEMENT
Types of nurseries
It is a place where young plants are raised under intensive care for later
transplanting to field.
Seeds Act and the Nursery Registration Act have been in operation since
December 1966.
Nursery Registration Act is presently in force in respect of horticulture
nurseries only in the States of Punjab, Maharashtra, Himachal Pradesh, Uttar
Pradesh, Uttrakhand, Jammu and Kashmir, Orissa and Tamil Nadu.
Only 30-40% demand of planting material is being met by existing
infrastructure.
Temporary nursery- Flying nursery.
26 Essence of Horticulture
8. GREENHOUSE STRUCTURES
Presently, China is the world leader in cultivating horticulotural crops under
protected condition.
Polyhouses gives 5-6 times higher production compared to open field.
Temperature differential between indoors and outdoors is greatest at roof.
Glass houses are very popular in the Netherlands.
‘Sea of Plastics’, a 50,000 acre world’s largest complex of a series of
greenhouse is located at Almeria in Spain.
Greenhouse structures should be designed to resist a 130 km/hr wind
velocity.
Aluminum and hot dipped GI are comparatively maintenance free.
Chromated copper arsenate and ammonical copper arsenate are water based
preservatives that are applied to the wood that may come into contact with
the soil.
Red wood or cypress (natural decay resistance woods) can be used in desert
or tropical regions, but they are expensive.
NCPAH: National Committee on Plasticulture Applications in Horticulture,
1981.
CPCT: Centre for Protected Cultivation, IARI, New Delhi.
PDC: Plasticulture Development Centre.
PFDC: Precision Farming Development Centre.
Lux: Unit for measurement of light.
Open roof green house was developed by Art Van Wingerden in 1990.
Open green houses protect from rain and hail.
Polyethylene film: Widely used in green house.
Low cost or naturally ventilated green house: Suitable for cucumber.
Walk in tunnel (low tunnel): Low cost, unheated, semi-permanent structures
e.g. cucurbits.
Polyethylene having 150-200 µ (800-1000 gauge) thickness is generally used.
Acrylic sheet is highly weather resistant and does not yellow with age.
The most recent addition of greenhouse film plastic covering is Tefzel T2
film (ethylene tetrafluoroethylene). Anticipated life expectancy is 20 years.
The light transmission is 95% and is greater than that of any other
greenhouse covering material.
Insect net proof houses used for virus free crop production: Tomato &
sweet pepper.
General Horticulture 27
Media
Rockwool was discovered in 1969 by Bovre and Knoblauch.
Perlite and sphagnum pea moss holds 3-4 times its weight in water.
Coco pith: Good drainage and porosity.
Perlite: Neutral (volcanic origin) & fastest uptake rate of nutrients.
Peat: Widely used & acidic in nature. It has good air and water holding
capacity.
Vermiculite: Micaceous mineral; chemically hydrated magnesium aluminium
silicate. Rich in Ca and Mg.
Rockwool: Aeration to roots.
Sphagnum moss or peat moss: High water holding capacity.
pH of sphagnum moss: 3.5-4.0.
General Horticulture 31
Molecular markers
Serological and PCR based diagnostics were developed for banana, citrus,
grapes, black pepper.
Concept of association of marker with quantitative traits was 1st proposed
by Sax in 1923.
1st concept of genetic map was presented by Alfred H. Sturtevant, 1913.
1st genetic map published in 1911 by T.H. Morgan
1st molecular marker: RFLP, 1980
To minimize linkage drag, need to identification of flanking marker: <5
cM.
Term marker assisted selection (MAS) was first used by Beckmann and
Soller, 1986.
Southern hybridization: DNA-DNA hybridization. Developed by S.M.
Southern (1925)
PCR: Polymerase Chain Reaction. It was developed by Dr. Karry Mullis in
1985.
In PCR, multiplication of DNA takes place by geometric progression.
PCR proceeds in 3 distinct steps governed by temperature as denaturing,
annealing and synthesis.
Thermostable enzyme in PCR: Taq (Thermus aquaticus ) DNA polymerase. It
is soil bacterium extracted from hot springs of sulphur.
Morphological marker: Seed colour, leaf shape, Seed size
DNA marker: PCR based markers e.g. RAPD, AFLP, SSR
SDS-PAGE: Poly Acrylamide Gel Electrophoresis. It separates proteins based
on their charge and molecular weight ratio.
Dominant marker: RAPD, AFLP, ISSR, SCAR
Co-dominant marker: RFLP, SSR, SRAP, STS, SNP, STS
First generation marker: RFLP
Second generation marker: RAPD, AFLP, STS, SSR
Third generation marker: SNP
Genome scanning for expressed genes: EST, SRAP, TRAP
Highly informative marker i.e. very high polymorphism: SSR (microsatellite)
Marker system used for identification of clones: CAPS and SNP.
Markers using array technology: Microarray, DArT (whole genome
scanning).
General Horticulture 39
Expressed Sequence Tags (ESTs) are used to make DNA marker in gene
discovery research.
NIL: Near Isogenic Lines are developed through repeated random
backcrossing.
NIL: Commonly used for mapping QTLs.
RIL: Recombinant Inbred Lines are homozygous selfed or sib mated progeny
of individuals of an F2 population up to F7-F8.
DH: Double haploids.
Haploid plants in tomato using anther culture: Greshop and Doy (1972).
True breeding lines or permanent/ immortal populations: RIL and DH.
Shortest method to detect QTLs is BSA (Bulked segregant analysis).
Marker Assisted Selection (MAS) term coined by Hospital and Charcosset
(1997).
Marker Assisted backcrossing (MABC) marker used to assess the presence
of introgressed gene or QTL.
MABC term coined by Tankley (1983).
Background selection: Markers used to accelerate the return to recipient
parent genotype at other loci.
The strategy was proposed by Hillel (1990).
Physical map is measured by base pairs (bp).
National Genomic Resources Repository Conserves- Cloned genes.
Hairy root culture for secondary metabolite production are induced by
transforming plant cells with Agrobacterium rhizogenes.
Mg2+ ions are required for type II restriction enzymes.
Linkage map
Genetic map is representation of genes on chromosome arrayed in linear
order with distances between loci expressed as parent recombination (map
unit, centi-morgan).
It is measured in centi-morgans (cM).
One linkage map unit is 1% recombination.
One map unit= one centi morgan= 1% recombination between loci.
Comparative mapping used for analysis of genes and QTLs.
Orthologous gene: Genes in different species that originated by vertical
descent from a single gene of last common ancestor.
40 Essence of Horticulture
QTL mapping
Term QTL first coined by Gelderman (1975).
Concept of quantitative genetics: Fisher, Wright, Haldane
Most common method of QTL mapping is interval mapping.
Ideal cM distance for QTL cloning is 2 cM or less.
Fine mapping of high resolution QTL: 25-100 Kb
First tagged QTL in plants: fw2.2 (fruit weight in Tomato).
Genomics
Sum total of all an individual organism’s genes.
Study of DNA (genotype), mRNA (transcriptome), protein (proteome) level.
Term genomics was coined by Dr. Tom Roderick
Structural genomics: Describe 3D structure of every protein encoded by
given genome.
Functional genomics: Understanding the function of genes and other parts
of genome.
Proteomics: A complete set of protein present in a single cell.
Transcriptomics: A complete set of mRNA present in single cell.
Metabolomics: A complete set of metabolites present in single cell.
Tranplastomic: Transfer of chloroplast.
Comparative genomics: Study of similarities and differences in structure
and function of hereditary information across taxa.
Sequencing technologies
1st bacterial genome sequenced: Haemophilus influenzae.
1st multicellue organism sequenced: Caenorhabditis elegans.
1st plant sequenced: Arabidopsis thaliana.
1st crop plant sequenced: Rice.
1st non-grassy plant sequenced: Banana.
2nd generation sequencing: Illumina system and Pyro-sequencing e.g. Roche
GS20, Roche GS FLX, SOLiD system and Sanger sequencing.
3rd generation sequencing: True single-molecule sequencing (tSMS).
General Horticulture 41
Transgenic activities
Crop(s) Gene(s) Function(s)
Biotic stress
1. Fungi
Tomato & Brinjal Chitinase, glucanase & thaumatin Fungal resistance
Tomato OXDC
Banana cv. Rasthali Ace-AMP-1 gene Fusarium wilt resistance
Banana HrAp gene from capsicum Fusarium wilt resistance
Pomegranate Plant ferredoxin protein gene Bacterial blight resistance
Xa21 from rice Nodal blight resistance
2. Virus
Banana BBTV, BBr, MV coat protein gene Virus resistance
Citrus CTV coat protein Virus resistance
Papaya PRSV coat protein Virus resistance
Watermelon WMV coat protein Virus resistance
Potato Coat protein Potato virus Y (PVY)
resistance
Tomato Replicase gene Tomato leaf curl virus (TLCV)
resistance
Ni gene Nematode resistance
3. Insects
Tomato Cry 1Ab Fruit borer resistance
Brinjal Cry 1Ab Shoot & fruit borer resistance
Cauliflower Cry 1Ab Diamond back moth
resistance
Cabbage Cry 1Hc/ Cry 9C Lepidopteron insect
resistance
Potato Cry 1Ab Potato tuber moth resistance
Squash Coat protein Virus resistance
Abiotic stress
Potato Osmotin Water stress tolerance
One gene from potato itself Reduced conversion of sucro-
se to glucose & fructose leadi-
ng to reduced acrylamide
Sugar beet Phosphino thricin acetyl Herbicide tolerance
transferase, Enolpyruvylshikimate
-3-phosphate synthase
44 Essence of Horticulture
Transgenic varieties
Crop Trait Product name Year Organization
Tomato Shelf life Flavr Savr 1994 Calgene
Shelf life Endless Summer 1995 DNA plant tech.
Potato Bt gene (resistance to New Leaf 1995 Monsanto Co.
Colorado beetle)
Resistance to Colorado beetle Elizabeth 2010 Monsanto Co.
Resistance Bt gene & potato New Leaf Plus 1998 Monsanto Co.
leaf roll virus (PLRV)
Resistance Bt gene & potato - 1999 Monsanto Co.
virus Y (PVY)
Bt gene Maximize 1995 CIBA-GEIGY
Resistance to viruses Freedom II 1995 Ashgrow Seeds
Papaya Resistance to PRSV Rainbow, Sun Up 1998 -
15. NANOTECHNOLOGY
Term ‘nanotechnology’ was coined by N. Taniguichi in 1974 at Univ. of
Tokyo, Japan.
It is understanding and control of matter at dimension of 1-100 nm.
Example of Nano based Small Delivery System: Halloysite.
General Horticulture 47
Nano pesticide: Nano particles of ZnO, SiO2 and TiO2 used for bacteria and
green algae.
Nano particles used for reclamation of heavy particles: Amphiphylic
Polyurethane, Zeravalent Iron and Zeolite.
‘Nano-encapsulation’ can be used to improve the insecticidal value.
Chile: Leading country in use of nanotechnology in post harvest
management of fruits.
Artificial seeds
Term ‘artificial seed’ was coined by Murashige in 1977.
Commonly used hydrogels: Sodium alginate, Carrageenan, Guar gum,
Sodium pectate, Tannin acid, copper sulphate, etc.
B
Fruit Science
1. GENERAL POMOLOGY
Word pomology: Derived from Greek word ‘pome’ meaning development
of fruit from inferior ovary and ‘logos’ meaning to study.
Father of systematic pomology: De Candolle.
Temperate fruits are popularly known in India as hill fruits or cold region
fruits.
India ranks 1st in productivity of grapes, banana and papaya.
Ratio of Chlorophyll a to chlorophyll b is highest in C4 plants.
Gibberellic acid is enhanced by exposing plants to red light.
The life line tree of ‘Thar Desert’ is Prosopis cineraria.
Grape of the desert is Salvadora oleoides.
National phytotron facility is located at IARI, New Delhi in 1997.
South-west direction is most sensitive to sun burn effects in plants.
Lopping of rootsock: Removal of upper part of root stock after setting of
buds.
Best time of planting temperate fruits: December-January.
Resinous canals are characteristic features of family Anacardiaceae.
Degreening temperature for most horticulture crops: 270C.
Bisexual flowers are also known as ‘bisporangiate’.
Photoblastic seed germination is observed in raspberry.
Trichoderma viride is used to control damping off.
Constant factor for estimating heat units is 10ºC.
In pome fruit species, short shoots are named ‘dards’ when they are strictly
vegetative.
Sweet chestnut has highest longevity.
Seed of joboba (Simmondsia chinensis) is rich in oil (45-55%) content.
Winter flowering in sub-tropical fruits create frost damage.
50 Essence of Horticulture
Layout of Orchard
Planting of trees should be such that intercept maximum solar energy.
Every 3rd tree in every 3rd row should be pollinizer in self incompatible
varieties.
Evergreen tree should be planted in the front and deciduous one behind.
Total orchard area under roads, buildings, paths, tube-wells is 10%.
Filler plants must be quick growing and early fruiting.
Suitable plants for fencing: Agave, Pithecolobium dulci, Thevetia peruviana.
Suitable hedge plants: Duranta plumier, Lantana camera, Tecoma stans, Prosopis
julifera, Opuntia spp., Clerodendron inerme, Inga dulcis.
Windbreaks have its effectiveness for a distance about 4 times its height.
Windbreaks are planted in north west side of orchard.
Fruit Science 51
Systems of Planting
1. Square system: Simplest and ubiquitous system of planting. More commonly
followed.
2. Rectangular system: Used in high density planting (HDP) e.g. Grape, Phalsa
and Peach.
3. Cluster system: Accommodates nearly twice the population of square system.
4. Triangular: Accommodates 11% lesser number of plants than square system.
Mostly used in high density planting (HDP). Intercultural operations
difficult.
5. Hexagonal/Equilateral triangle: Accommodates 15% more plants than square
system. Very difficult to layout.
6. Quincunx/Diagonal: Most suitable in highly fertile soils. Accommodates
1.5-2 times more plants than square system. Adopted when tree spacing
exceeds 10 m or slow growing fruit tree. Filler plants are generally used
e.g. Banana, Guava, Kinnow, Phalsa, Papaya, Peach, Pomegranate and Plum.
7. Contour system: Followed in hills & undulated land (slope >10%).
8. Terrace system: Extension of contour system. Double hedge contour system
accommodates 22% higher than single hedge system. Hedge row planting
system is commercially followed in mechanized fruit cultivation e.g. Apple,
Pineapple.
9. Double/Hedge row system: Very intensive form of rectangular system.
Distance between trees in a row is half to one third the distance between
rows e.g. pineapple.
10. Full field system: Most efficient system for maximum light interception.
Training Systems
Training: done to maintain proper shape, canopy of tree and develop good
frame work.
Training controls distribution of fruiting wood and has little effect on fruit
bearing.
Multi storyed cropping is practiced in Karnataka and Kerala.
The ‘Tatura trellis’ system of training was 1st developed at Australia.
Free Air Temperature Increment (FATI) technology: Increase in canopy
temperature in fields.
Methods
1. Central leader system is also known as closed centered one.
2. Open centre system is also known as vase shaped system.
3. Modified leader: Most widely adopted/accepted for commercial fruit
cultivation.