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CHEMISTRY PROJECT
FREEDOM CONCEPT SCHOOL

TOPIC :
CHECKING THE BACTERIAL
CONTAMINATION IN DRINKING
WATER BY TESTING SULPHIDE ION.

DONE BY,
R. Gokul

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CERTIFICATE

This is to certify that this project on the topic

checking the bacterial contamination of drinking
water by testing sulphide ion has been
successfully completed and submitted by Gokul.R
of Class XI during the session 2023-24 under the
guidance and supervision of Mrs. Samundeshwari
to the Department of Chemistry.

Date: 01.01.2024

Internal Examiner External

examiner

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Principal

ACKNOWLEDGEMENT

I would like to express my special thanks of

gratitude to my chemistry teacher Mrs.
Samundeshwari for her able guidance and
support in completing my project.

I would also like to extend my gratitude to

Principal Sir Mr. Siva Kumar for providing me
with all the facilities that were required. I am
also very thankful to my parents who helped me in
my investigatory project, without whose
motivation and help the completion of this project
would not have been possible.

Gokuk.R

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CONTENTS

i) INTRODUCTION

ii) AIM

iii) REQUIREMENTS

iv) PROCEDURE

v) OBSERVATION

vi) RESULT

vii) CONCLUSIONS

viii) PRECAUTIONS

ix) BIBLIOGRAPHY

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INTRODUCTION

The sulphide ion test for bacterial contamination

is good. In surface waters, hydrogen sulphide is
formed under oxygen deficient conditions.
Hydrogen sulphide is also produced from the
decomposition of sulphur containing organic
compounds. The concentration of sulphide ion
becomes significant only at pH 10 or above.

Under acidic conditions, the concentration of

hydrogen sulphide predominates. Hydrogen
sulphide is a weak acid, which ionizes to yield
hydrosulphide (HS) and sulphide (S2) ions.
Hydrogen sulphide is highly toxic to fish.
Concentrations of total sulphide as low as 0.01
mg/L make the water uninhabitable for fish
culture. Hydrogen sulphide also reduces the
aesthetic value of the water body due to foul

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color. The bacterial contamination can be tested

using H2S strip.

Presence of pathogenic bacteria in water can be

detected by testing the sulphide content of
water. The presence of sulphide ions in water is an
indicator of: i) High pH above 10. ii) Sulphide
producing bacteria. iii) Diminished oxygen
concentrations.

Conducting the sulphide ion test :- The sulphide

ion test is conducted using a Hydrogen sulphide
strip (H₂S) - The H₂S strip is dipped into the
water to be tested for 14 to 16 hours. - The H₂S
strip turns black if sulphide ions are present in
the water. The black color is due to the ionization
of the H₂S.

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AIM

To test the contamination of drinking water by

bacteria by checking the sulphide ion
concentration and find out the cause of
contamination.

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REQUIREMENTS

Apparatus Required
i) Hydrogen Sulphide (H2S strip)

ii) Beaker

iii) Drinking Water

iv) Thermometer

Chemicals Required
i) Hydrogen Sulphide (H2S)

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PROCEDURE

This experiment is to be done in an incubator.

✓ Add about 250ml of water in a beaker

✓ Dip the H2S strip in the beaker with water.
✓ Heat the beaker for about 35 degree
centigrade.
✓ Check the temperature (temperature should
be at 35 degrees centigrade).
✓ Keep it in incubator for 14-16 hours of time.
✓ Check the result if the water turns black
then
it is unfit for drinking / highly contaminated
for drinking or for human use.
✓ The sulphide ion test for bacterial
contamination is good. In surface waters,
hydrogen sulphide is formed under oxygen-
deficient conditions. Hydrogen sulphide is also
produced from the decomposition of sulphur

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containing organic compounds. The

concentration of sulphide ion becomes
significant only at pH 10 or above.

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OBSERVATION

Source of Water Result

Water from the tap of Water is fit for

houses in my locality drinking.
Water from the public Water is unfit for
handpump drinking.
Spring Water Water is fit for
drinking
River Water Water is not fit for
drinking

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RESULT

By the experiment done the result says that at

some place water is fit for drinking whereas it is
unfit for drinking at some places. Especially water
is unfit for drinking from river bodies.

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CONCLUSION

✓ Water is mostly unfit for drinking from

river.

✓ Water that come to houses is also unfit for

drinking at some of the places.

✓ Therefore, we should purify the water

before
drinking. And we should not drink water from
random sources.

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PRECAUTIONS

✓ Use same volume of water sample from

different sources.

✓ Same quantity of reagent should be used for

different samples.

✓ Do not taste the water sample.

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BIBLIOGRAPHY

i) Chemistry Lab Manual

ii) www.brainly.in
iii) www.quora.com
iv) www.ncert.nic.in
v) www.coursera.com

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