Biochem Lab Practice Exam PDF

: ✓ = Vmax
Michaelis Menten
-
equation S)
Kmt [
BCHS3201 Practice Exam

y intercept
-11
-
Vmax
X -
intercept =
11km
1. In the plot above, “a” represents

a. -1/KM
b. 1/Vmax
c. KM/Vmax
d. KM
e. Vmax/2
2. How many grams of MgCl2 would I need to make 25 ml of a 10 mM MgCl2 solution (M.W. 59.76)?
-
- -
-
a. 14940 g ( formula weight) ( Volume desired ) ( molarity desired ) = g needed

b. 14.94 g glmol L M (Moll L )
c. 1.494 g
0.025 L) ( 0.01 M )
d. 0.1494 g ( 59.76 ) (
e. 0.0149 g 0.01494-9 =
18.02 91 Mol
3. If you mix 300 ml of 90 mM NaCl (M.W. 58.5) with 72 ml of water, what is your final
- - - - -
concentration of NaCl in mM? Cl VI

- - CZVZ =
a. 47.48 mM
( 90mm) ( 300mL) ( C 2) ( 300+72 ML) =
b. 58.5 mM
c. 72.58 mM (z 72 58 MM =
-
.
d. 90 mM
e. Impossible to solve with the information provided
4. You have 300 ml of a 150 mM solution of phenylalanine (M.W. 165.2). Express the solution
- -
concentration as a %. weight volume ( L) molarity ( m )

-
-
g formula
-
a. 0.073% 165.2 =
O 3 O 15 o
7.4349
.
.
-
=
b. 7.3%
c. 0.024%
d. 2.43%
t.gg#--,ofmcx=2.-48Yo
e. Impossible to solve with the information provided
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5. A protein is composed of 4 subunits. Three subunits have a molecular weight of 35 kDa.
-
- -
One subunit has a molecular weight of 110 kDa. The protein is completely denatured with
- -
reducing agents, heat, and SDS. How many bands should you see on an SDS-polyacrylamide
-
gel stained with Coomasie Blue?

a. One * SDS PAGE separates based on site ( & charge) .
b. Two ( 2 different sizes 2 different

= bands ) .
c. Three
d. Four
e. Impossible to determine with the information given.
6. A protein is composed of 6 subunits. Two have a molecular weight of 55 kDa, two have a
-
- - -
molecular weight of 75 kDa, and 3 have a molecular weight of 95 kDa. How many bands should
- - -
you see on a native polyacrylamide gel stained with Coomasie Blue? rate
factor Affecting migration
- .
: site is not the only

* native gels
a. One
b. Two
c. Three
d. Four
e. Impossible to determine with the information given.
7. Which type of paper will a researcher use to report the results and details of their wet-bench
experimental work to the scientific community?
a. Review of literature
b. Research article
c. Editorial
d. newspaper release
Native SDS PAGE
1 2 1 2
8. In the gels pictured here, lane 1 is a protein molecular weight marker (values are in kDa). Lane 2 is
the Notsosnazzy protein. Assuming this protein is composed of 2 subunits, what is the molecular weight
-
of this protein? )
( from SDS page
Subunits 30 kDa
2 ,
a. 52 kDa = 60 KDa
b. 104 kDa
c. 30 kDa
d. 60 kDa
SDS PAGE
1 2 3 4 5
SO
36
9. A researcher is working with protein bchsrocks which is composed of the following subunits:
A (16 kDa), B (20 kDa), C (50 kDa). Subunits A and B are tethered with together with a
- - -
disulfide bond. Which lane on the pictured gel represents what the researcher would see if they
denatured the protein with heat but forgot to add the beta-mercaptoethanol? Lane 1 represents a
- -
molecular weight marker (kDa). bonds

separates disulfide
ethanol :
beta mercapto
-
a. lane 2 kDa )
b. lane 3 -
Should see : 2 bands (I 36 kDa , I 50
c. lane 4 ↳
Laine
d. lane 5
-
-
-
÷
10. If the plasmid pictured is cut with EcoRI and SalI, what size bands would you expect to see
on a 1% agarose gel? Assume the digest goes to completion.
-
}
8617 6592=2025 -
5978
a. 4335 bp, 2010 bp, 1943 bp, 2025 bp 4649=1943
6592
-
b. 5978 bp, 2010 bp, 1943 bp, 2025 bp 2010

4649 2639
=
c. 2639 bp, 4649 bp, 6592 bp, 8617 bp

-
5978 4335 =
d. 6360 bp, 1943 bp, 2010 bp, 1013 bp 10313

-
@
e
11. The plasmid pictured is cut with SalI but the digest does not go to completion. Which lane
-
in the pictured gel represents this scenario? Lane 1 is the Lambda-HindIII DNA ladder (marker)
a. Lane 2 not every variation of
going to completion
=
band Sizes will
b. Lane 3 be expressed .
c. Lane 4
2 out sites 6 band sizes
=
d. Lane 5 *
12. In the virus map above, all of the HindIII restriction enzyme sites are marked. What is the
-
maximum number of bands you could see on an agarose gel if the digest does not go to
-
-
completion?
-
a. three
b. four
c. five
d. six
e. impossible to determine
* LOOK over instructions
microarray
13. For your microarray analysis, the present (P), absent (A), and marginal (M) calls were converted to
values of 2, 0, and 1 respectively. Why?
a. Numeric values can be averaged across replicates of the experiment. Letter values cannot be
averaged.
b. All zero values need to be removed from the data set as the gene was never expressed.
-c. It allows the list of genes to be organized from most highly expressed to least expressed.
d. It provides a code for looking up information on the gene in TAIR.
-
14. In a microarray experiment, a T-Test is used to

-
a. determine the Present/Missing/Absent (PMA) call.

b. determine if there is a statistically significant difference in gene expression between
the control replicates.
c. determine if there is a statistically significant difference in gene expression between
the experimental replicates.
d. determine if there is a statistically significant difference in gene expression between
the control and experimental replicates.
15. What is collected from a sample for use in a microarray experiment?

a. DNA
b. mRNA
c. protein
d. metabolites
16. (Choose all that apply) In an SDS-PAGE (polyacrylamide) gel, proteins migrate based on
their nativegesi
100K Over 3D conformation
a. 3 -dimensional conformation
-
charge
PAGE / WB
-
b. charge c. , -
size
HW t Info c. size
d. hydrophilicity ( one band )
17. A researcher wants to compare how much myosin protein is present in samples of types of
shellfish muscle tissue. All muscle tissue contains myosin. Muscle tissue from shrimp, lobster,
-
crabs, and crawlfish are prepared and loaded into separate wells on a polyacrylamide gel.
Electrophoresis is conducted and the gel is stained with Coomassie blue. Which statements
below are true (select all that apply).
a. Only one single blue band corresponding to myosin protein will be present in each
lane.
b. Each lane will contain multiple blue bands representing the array of proteins present
in fish muscle tissue.
c. No bands will be visible unless the proteins are transferred to a blot, probed with
antibody specific to myosin, and subjected to colorimetric detection.
d. Multiple protein bands will be visible after the proteins are transferred to a blot,
probed with myosin antibody, and subjected to colorimetric detection.
18. Which has greater resolving power?

greater resolving power
higher to
=
a. 1% agarose gel
b. 4% agarose gel
19. The graph below depict what type of inhibitor:
noncompetitive competition :
competitive inhibitor
Catt
:
#
Uncompetitive inhibitor :
-
O
O
a. Competitive inhibitor
}
know each
b. Noncompetitive inhibitor
c. Uncompetitive inhibitor graph ! *
20. The data for the Hill reaction and the kinetics lab was generated taking spectrophotometric
readings over time. What was directly measured by the spectrophotometer? -
a. production of oxygen
b. changes in a colorimetric detector
c. electron transport
d. enzyme activity
e. a and b are correct
f. c and d are correct
blue in Its oxidized State
.
DUP IS
* when it gains Ht , it becomes
clear .
21. Why do the absorbance values in the kinetics experiment increase with time?
- -
→ clear .
a. The DCIP turns from clear to blue when it gains hydrogen atoms from hydrogen peroxide in the
-
presence of peroxidase.
b. The DCIP turns from clear to blue when it donates hydrogen atoms to form water in the presence of
peroxidase and hydrogen peroxide.
c. The guiacol turns from clear to brown when it gains hydrogen atoms from hydrogen peroxide
in the presence of peroxidase.
d. The guiacol turns from clear to brown when it donates hydrogen atoms to form water in the presence
of peroxidase and hydrogen peroxide.
22. The substrate for turnip peroxidase in your kinetics experiments was
a. guiacol reducing agent ( donates Hts
-
KINETICS b. hydrogen peroxide gains Ht to make 2 H2O -
c. hydroxylamine
d. DCIP
/
e. water
23. Photosynthesis relies on an electron transport chain. How does the electron enter the chain?
-
Hill rxh . a. sunlight

2.Ht
KNOW b. splitting of water H2O →
photo -
c. donated by DCIP
synthesis
process .
d. donated by DCMU
24. Which type of gel do scientists use to analyze protein?

-
a. agarose gel
b. polyacrylamide gel
25. Sitting on the shelf is 10X gel running buffer. You need a 1X gel running buffer for your
- -
experiment. How much of the 10X gel running buffer do you need to make 5L of 1X gel -
running buffer? How much water?

IX -_ 0.1
-
10×3 IX
a. 500 ml 10X buffer+4500 ml water
b. 4500 ml 10X buffer + 500 ml water
Ic. 500 ml 10X buffer + 5L water 5.5 L
✓ d. 1 L 10X buffer + 5 L water 6L
oax
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: ✓ = Vmax

BCHS3201 Practice Exam

1. In the plot above, “a” represents

a. 14940 g ( formula weight) ( Volume desired ) ( molarity desired ) = g needed

concentration of NaCl in mM? Cl VI

concentration as a %. weight volume ( L) molarity ( m )

gel stained with Coomasie Blue?

b. Two ( 2 different sizes 2 different

: site is not the only

molecular weight marker (kDa). bonds

b. 5978 bp, 2010 bp, 1943 bp, 2025 bp 2010

c. 2639 bp, 4649 bp, 6592 bp, 8617 bp

d. 6360 bp, 1943 bp, 2010 bp, 1013 bp 10313

14. In a microarray experiment, a T-Test is used to

a. determine the Present/Missing/Absent (PMA) call.

15. What is collected from a sample for use in a microarray experiment?

18. Which has greater resolving power?

19. The graph below depict what type of inhibitor:

KINETICS b. hydrogen peroxide gains Ht to make 2 H2O -

Hill rxh . a. sunlight

24. Which type of gel do scientists use to analyze protein?

running buffer? How much water?

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