KOLEJ MATRIKULASI JOHOR

MINISTRY OF EDUCATION 84900, MARKS:

TANGKAK JOHOR

NAME NOR NAZURAH IZZATI BINTI SHAHRUN NIZAM

MATRIC NUMBER MS2316115779
PRACTICUM H2
LECTURER’S NAME PN KHAIRUL BARIAH BINTI SULAIMAN
DATE OF 21 FEBRUARY 2024
EXPERIMENT

TITLE 1 mark
CELLULAR RESPIRATION

OBJECTIVE 3 marks

i. To organize the experiment setting for redox reaction procedures;

ii. To Conduct an experiment on redox reaction in cellular respiration; and
iii. To explain the biochemical processes in yeast suspension.

INTRODUCTION 1. Explain the redox reaction.

reactions that involve the transfer of electrons from one species to another 1 mark

2. What is the substance in a living cell that has the same function as
methylene blue?
1 mark
Coenzyme, example as vitamin and NAD+
3. Name the important process which involves substance in question 2 above.
Oxidative phosphorylation
1 mark
PROCEDURE 3 marks
1. 3 boiling was labelled tubes A, B and C.
2. The tube was filled in with 10 ml of yeast suspension.
3. tube C was heated in boiling water for 5 minutes.
4. 5 drops of methylene blue was added into each of the boiling tubes using
Pasteur pipette. Shake gently to ensure the colour is evenly distributed.
5. The colour was observed on the yeast suspension in all boiling tubes.
Observation was recorded in Table 10.1.
6. all boiling tubes were incubated in the water bath at 40oC for 15 minutes.
7. The colour changes of the yeast suspension was observed in all boiling tubes.
observation was observed In Table 10.1.
8. tube B was placed in boiling water for 5 minutes.
(Note: Make sure the water boils first before placing tube)
9. The boiling tube A, B and C was plugged with a cork or rubber stopper. Press
it with thumb and was shaked the tube vigorously for 30 seconds. the
 colour changes were observed of the yeast suspension. observation was recorded
in Table 10.1.
10. The stopper was removed and incubated all boiling tubes in a water bath at
40oC for 15 minutes.
11. The colour of the yeast suspension was observed in each boiling tube.
observations were recorded in Table 10.1.
(Note: Observations are based on the colour changes.)

RESULTS

Treatments Colour
Tube A Tube B Tube C
Before Blue Blue Blue 1 mark
incubation (400C)
After 1 st Light Blue Light Blue Blue 1 mark
0
incubation (40 C)
After vigorous Light Blue Blue Blue
shaking 1 mark
After 2 nd
Light Blue Blue Blue 1 mark
incubation (400C)

DISCUSSION
Boiling Explanation
tube
 A After 15 minutes/1 incubation
st

● Yeast used all dissolved oxygen// conduct aerobic

3 marks
respiration
● When dissolved oxygen is used up, solution
colour becomes light blue.
● Because methylene blue is reduced to form
decolourised methylene.
Remarks
**But remain blue at air – water interface because
dissolved O at the upper part causes methylene
2

blue to oxidize
3 marks
After shaking
● Shaking causes oxygen from the air to dissolve
into the solution.
● Methylene blue is decolourized_.
● Changes the solution to light blue colour.
After 10 – 15 minutes/2 incubation
nd

Remarks
* Same explanation as in after 15 minutes

B After 15 minutes/1 incubation

st

Remarks
* Same explanation as in Tube A

After shaking
Remarks
* Same explanation as in Tube A

After 10 – 15 minutes/2 incubation

nd

● Boiling causes the enzymes (in yeast) to

denatured// yeast cells killed 3 marks
● Dissolved oxygen is not used.
● Methylene blue remains in blue form / cannot be
reduced
● The colour of solution unchanged / remain blue.

C ● No changes occur throughout the process. 2 marks

● Because boiling causes the enzymes in yeast to
denatured
● Dissolved O is not used, methylene blue is in
2

oxidized form / remain unchanged

CONCLUSION 1. Methylene blue act as artificial electron acceptor showing

redox reaction has taken place due to the colour changes. 1 mark

2. Methylene blue is reduced to form decolourised methylene

1 mark
3. Methylene blue is oxidized to change the solution to blue
colour
 23 February 2024 1 mark

Reference 1 mark
Christopher Spohrer.
https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_M
odules_(Analytical_Chemistry)/Electrochemistry/Redox_Chemistry/Oxidation-
Reduction_Reactions (Retrieved on 22 February 2024)
Submission date (not 1 mark
late)
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