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dihybrid crosses
biodiversity
- Number of species present in the biosphere. (number of species and their relative
abundance to each other).
- Types of biodiversity
o Genetic diversity
the total number of genetic characteristics in the genetic makeup of a
species
o chemical diversity
Different species produces a variety of chemicals in their cell. (variety of
metabolic compounds in an ecosystem)
o Ecosystem diversity
The number of different ecosystems in the planet or a given geographic
area.
Organelle-> cell -> tissues -> organs & organ system -> Organism, population,
communities -> ecosystem -> biosphere
fish:
o Single circuit for blood flow,
o 2 chambers (atrium: collects blood returned, ventricle(pumps blood to the
gills)
o Gill circulation: Where gas exchange occurs, and blood is re-oxygenated.
(occurs in the gills).
Human:
o Two circuits (double circulation), one through the lungs and one back to the
heart.
o 4 chambers (2 atria, 2 ventricles)
o Oxygenated blood is separated from deoxygenated blood.
Ecological relationships
Ecology: Is the study of interactions of living organisms with their environment.
Biological community: consists of the different species within an area, typically a three-
dimensional space, and the interactions within and among these species
Ecosystems
An ecosystem is a community of living organisms and their interactions with their abiotic
(nonliving) environment
Equilibrium: is the steady state of an ecosystem where all organisms are in balance with
their environment and with each other.
Resilience is the speed at which an ecosystem recovers equilibrium after being disturbed.
Food chains:
o Primary producer: at the bottom of the food chain. Photosynthetic organisms.
o Primary consumer: consumes the primary producer.
o Secondary consumer: carnivores. Eat primary consumers
o Apex consumer: highest level consumer in the ecosystem.
Holistic ecosystem model: quantify the composition, interaction, and dynamics of entire
ecosystems; it is the most representative of the ecosystem in its natural state.
Models include: 1- A conceptual model, 2- An analytical model, 3- simulation model
o Mesocosm: an experimental tool that is used as a model of a larger ecosystem
o Microcosm: artificial, simplified ecosystems that are used to simulate and predict
the behaviour of natural ecosystems under controlled conditions.
Energy acquisition
o Photoautotrophs: ex. Plants, algae serve as the energy source for a majority of the
world’s ecosystem.
o Chemoautotrophs: synthesize complex organic molecules ex. Glucose. For their
own energy without sunlight, rather use other sources of energy.
o Heterotrophs: acquire energy from digesting living or previously living
organisms.
Community dynamics
Are the changes in community structure and composition over time. (Introduced by
environmental disturbance ex. Volcanoes…).
Communities with stable structures are at equilibrium. (after the disturbance,
communities may or may not return to equilibrium)
Successions
Succession describes how the structure of biological community change over time.
o Primary succession: newly exposed or newly formed land is colonized by living
things. (when new land is formed or rock is exposed ex. Eruption of volcanoes, as
lava flows into the ocean, newly land is being formed)
o Secondary succession: part of an ecosystem is disturbed, and remnants of the
previous community remain. (wildfires good and bad)
Phylogenetic (there is no tree on the exam, but you should pay attention to how the basic tree of
life is constructed)
summarizes the evolution of various life forms on Earth.
Shows evolutionary relationships between species. (based on similarities and differences
in genetic or physical traits)
Atmosphere
It is the envelope of gasses surrounding the earth.
o The water cycle
o Carbon cycle
o Nitrogen cycle
o The phosphorous cycle
o The sulfur cycle
How organisms make energy
- All light is energy
- Energy acquisition
- Energy is the ability to do work
o energy is needed to carry out life processes (required to break down and build up
molecules, and transport many molecules across plasma membrane)
o the energy that organisms need comes from food, food consists of organic
molecules that store energy in their chemical bond.
o Two types of organisms to obtain food for energy: autotrophs and heterotrophs.
Energy molecules: glucose and ATP
o The two types of molecules of chemical energy.
o Key players in the process of photosynthesis.
Glucose: is a simple carbohydrate (C6H12O)
It stores chemicals energy in a concentrated stable form.
Carried in the blood.
End product of photosynthesis
ATP: the energy-carrying molecule that cells use to power cellular process
Made in the first half of photosynthesis, then used for energy for
the second half
It releases energy when it gives up one of its three phosphate
groups.
The breakdown of the ATP is a catabolic reaction (break down
large organic molecules into smaller molecules) that releases
energy (exothermic)
The makeup of ATP is ADP+Pi, an anabolic reaction (synthesize
larger molecules from smaller constituent parts) that takes energy
(endothermic).
Why organisms need both glucose and ATP?
The energy flow through living things
Photosynthesis glucose
In a process called cellular respiration (metabolic reaction, convert
biochemical energy from nutrients into ATP), organisms break
down glucose and make the ATP they need.
Entropy
Entropy is a measure of randomness or disorder in a system
The second law of thermodynamics states that every energy transfer or transformation
increases the universe's entropy.
o Systems can be thought of as having a certain amount of order.
o It takes energy to make a system more ordered.
o The more ordered a system is, the lower its entropy.
o High disorder, higher entropy (energy lost)
Linnaeus did something…. What?
- Taxonomy – science of classifying organisms into taxa
- His work in taxonomy: the science of identifying, naming and classifying organisms.
- Linnaean system
- Top: domain kingdom phylum class order family genus species
o Genes are used to make mRNA by the process of transcription mRNA is used
to synthesize protein by a process called translation.
Transcription process:
Initiation: a promoter that indicated where RNA polymerase
should bind to start
Elongation: RNA polymerase tracks along the DNA template to
split DNA and make mRNA. From 5’ to 3’
Termination: liberates the mRNA that happened on elongation
(Rho protein). A ribosome will grab onto the growing mRNA to
start translation.
Difference between eukaryotes and prokaryotes is that since there is no nuclear
membrane in prokaryotes, there is no separation of the transcription and translation
process.
Translation process:
Initiation: AUG start codon then tRNA attaches. Then larger
subunit comes in.
Elongation: tRNA is moving in with the right amino acids. Amino
acid chain growing.
Termination: stop codon is reached.
In eukaryotes, the amino acid is moved into E (endoplasmic
reticulum) to further process.
o mRNA processing:
mRNA contains introns that must be spliced out. A 5' cap and 3' poly-A
tail are also added
These structures (5’ and poly-A tail) protect the mature mRNA from
degradation and help export it from the nucleus
Pre-mRNAs also undergo splicing, in which introns are removed and
exons are reconnected with single-nucleotide accuracy.
Only finished mRNAs that have undergone 5' capping, 3' polyadenylation,
and intron splicing are exported from the nucleus to the cytoplasm.
o Gene families: a set of several similar genes, formed by the duplication of a single
original gene.
pools
o Gene pool: all the alleles that the individual in the population carries.
Alleles
o It is one or more versions of a gene. Individuals inherit two alleles.
Dominant: alleles that mask others – often designated with capital letters.
Example: P for purple flower
Recessive: alleles that are masked by others – often designated with lower
case letters Example p for white flower
Genotype vs phenotype
Genotype: is an individual’s collection of genes
Phenotype: the observable trait expressed by an organism.
- Complete dominance
the dominant allele completely masks the effect of the recessive allele in
heterozygous conditions
Photosynthesis overview (it would behoove you to know the difference between PSII and PSI)
- CO2 + H2O C6H12O6 + O2
- photosynthesis takes place inside the chloroplast
- two parts of photosynthesis:
o light dependant reaction
in the thylakoid membrane
use light energy to make ATP and NADPH
a photosystem consists of a light-harvesting complex and a
reaction center.
o Photosystem II
Pigments in the L-HC pass light energy to two special
chlorophyll a molecule in the reaction center.
Light excites the chlorophyll a pair and passes it to the
primary electron acceptor, PEA, it looks like pea.
The excited electron must be replaced. The electron
comes from the splitting of water, which releases
oxygen as a waste product.
o Photosystem I
Receives electron from ETC
Uses light energy to transfer then across the
membrane
from CO2
it has three stages
the enzyme RuBisCO incorporates carbon dioxide into an organic
molecule, 3-PGA
The organic molecule is reduced using electrons supplied by
NADPH
RuBP is the molecule that starts the cycle. It is regenerated so that
the cycle can continue (bc it’s a cycle)
Only one carbon dioxide molecule is incorporated at a time, so the cycle
must be completed three times to produce a single three-carbon G3P
molecule. And six times to produce a six-carbon glucose molecule.
Gibb’s
Thermodynamic laws
- energy cannot be created nor destroyed. it only can be stored and moved
- energy cannot be transferred (not entirely efficient. when energy is lost it will result with
increased entropy)
if a solution has a pH of 8, it has less free hydrogens.
Enzymes
Enzymes are protein* catalysts that speed up reactions by lowering the required
activation energy.
- Describe the evolutionary relationship between heat and enzymes?
The more enzymes work the more heat they produce. The hotter the system becomes. If
they work too much they get destroyed. If they work too little, they don’t work efficiently
enough.
- Why is it important for an organism to have a homeostatic pH and temperature?
So their enzymes work. Otherwise life can’t happen.
Types of enzymes:
o Catabolic – breakdown substrates
o Anabolic – build more complex molecules
o Catalytic – affect the rate of reaction
How Enzymes lower activation energy:
o position two substrates so they align perfectly for the reaction
o provide an optimal environment, i.e. acidic or polar, within the active site for
the reaction
o contort/stress the substrate so it is less stable and more likely to react
o temporarily react with the substrate (chemically change it) making the
substrate less stable and more likely to react.
Enzyme regulation
o Regulation of enzyme activity helps cells control their environment to meet their
specific needs. Ex. digestive cells in your stomach work harder after a meal than
when you sleep.
Enzymes can be regulated by
Modifications to temperature and/or pH
Production of molecules that inhibit or promote enzyme function
Availability of coenzymes or cofactors
Biogeography
- is the study of the geographic distribution of living things and the abiotic factors that
affect their distribution
- Abiotic factors such as temperature and rainfall vary based mainly on latitude and
elevation.
- As these abiotic factors change, the composition of plant and animal communities also
changes.
Demography
- The statistical study of population dynamics, demography, uses a series of mathematical
tools to investigate how populations respond to changes in their biotic and abiotic
environments.
Endemic
- Things or organisms found in only one location.
- Endemics with highly restricted distributions are particularly vulnerable to extinction.
DARWIN (remember, I think)
Premises of Darwinism is THE STRONG SURVIVE.
Best genetics, more adaptive survive.
How genes cross over and why do some genes cross together
- In meiosis
- paired chromosomes from each parent align so that similar DNA sequences from the
paired chromosomes cross over one another.
- Variation in alleles
-
Independent assortment
- During meiosis, the pairs of homologous chromosome are divided in half to form haploid
cells, and this separation, or assortment, of homologous chromosomes is random.
- genes do not influence each other with regard to the sorting of alleles into gametes, and
every possible combination of alleles for every gene is equally likely to occur
- can be illustrated by the dihybrid cross
Monohybrid crosses
Know it already
Mitosis vs meiosis
- Mitosis
o Phases
Interphase: time for normal growth and preparation for cell division
G1 (first gap): cell is activated
S: identical copies of DNA are joined at the centromere. Cell
synthesizes a complete copy of DNA in its nucleus.
G2 (second gap): energy is replenished, organelles reproduce, and
cytoskeleton breaks down.
Prophase: nuclear envelope breaks, microtubules form
Prometaphase
Metaphase
Anaphase
Telophase
Cytokinesis
Animal cell: a cleavage furrow separates the two daughter cells
Plant cell: cell plate
o Products of mitosis
two identical diploid (2n, has paired chromosomes) daughter cells
- Meiosis
o Meiosis I
begins with one diploid parent cell and ends with two haploid daughter
cells
Prophase I:
Prometaphase I:
Metaphase I:
Anaphase I:
Telophase I and cytokinesis:
o Meiosis II
starts with two haploid parent cells and ends with four haploid daughter
cells
Prophase I:
Prometaphase I:
Metaphase I:
Anaphase I:
Telophase I and cytokinesis:
o Products of meiosis
Four haploid cells in which each chromosome has just one chromatid. In
humans, the products of meiosis are sperm or egg cells.
What features of meiosis allow for an independent assortment of chromosomes? Crossing over?
the pairs of homologous chromosomes are divided in half to form haploid cells, and this
separation, or assortment, of homologous chromosomes is random.
o Checkpoints
G1: determines if all conditions are favorable for the division
G2: make sure that all chromosomes have been replicated and not
damaged.
M: determines whether all sisters chromatids are correctly attached to the
spindle microtubules.
o Cell cycle
Interphase
G1
S
G2
M (mitotic phase)
RNA poly 2
- RNA polymerase I: transcribes RNA
- RNA polymerase II: transcribes protein-coding genes
o Located in the nucleus
o Synthesizes all protein-coding nuclear mRNAs
o Undergoes extensive processing after transcription but before translation
o RNA polymerase II is responsible for transcribing the overwhelming majority of
eukaryotic genes
- RNA polymerase III: transcribes rRNA, tRNA (helps code mRNA to become a protein),
and small nuclear RNA genes
Intron splicing: removal of the non-coding introns.
Cellular respiration
- 4 steps:
o Glycolysis
o Intermediate- rearrangement (pyruvate oxidation)
Upon entering mitochondria
A multienzyme converts pyruvate into acetyl CoA.
Carbon dioxide is released
One molecule of NADH is formed
o Kerbs- citric acid cycle
the acetyl group from acetyl CoA is transferred to oxaloacetate to form
citrate.
Through a series of reactions
citrate is oxidized (3 NADH & 1 FADH2 made)
2 CO2 released
1 GTP or ATP produced
The final product of the citric acid cycle is oxaloacetate.
The cycle runs continuously in the presence of sufficient reactants
Products of CAC:
- 2 carbon dioxides
- ATP
- Reduced forms of NADH and FADH2
o Electron transport chain (Oxidative Phosphorylation)
The electron transport chain is a series of electron transporters embedded
in the inner mitochondrial membrane that shuttles electrons from NADH
and FADH2 to molecular oxygen. In the process, protons are pumped
from the mitochondrial matrix to the intermembrane space, and oxygen is
reduced to form water.
DNA vs RNA
Both DNA and RNA can store information, yet DNA is the major information storage
molecule in most cells. What are the advantages of using DNA for information storage
instead of RNA? DNA is more stable
DNA- stable RNA reactive
Double strand single strand
Remains in the nucleus leaves the nucleus
C, T, A, G C, U, A, G
Carries genetic information involved in protein synthesis
They both consist of monomers called nucleotides
o Proteins
Types and functions
Digestive enzymes: digestion of food by catabolizing nutrients into
monomeric units
Transport: carry substances in the blood or lymph throughout the
body
Structural: constructs different structures
Hormones: coordinate the activity of different body systems
Defense: protect body from foreign pathogens
Contractile: muscle contraction
Storage: provide nourishment in early development of embryo.
Amino acids are the monomers that make up the protein
o Fundamental structure
o Central carbon atom (α-carbon)
o Amino group (-NH2)
o Carboxyl group (-COOH)
o Hydrogen
o Side chain (R-group)
Different natures of amino acids:
o Nonpolar
o Polar
o Positively charged
o Negatively charged
o Nonpolar aromatic
The sequence and number of amino acids determine protein shape,
size and function
Protein structure/shape can change with altering primary structure
due to:
o Changes in pH
o Changes in temperature
Protein is multiple polypeptide (polypeptide is a chain of amino
acids joined by a peptide linkages)
Protein shapes
Primary structure: the unique sequence of amino acids in a
polypeptide
Secondary structure: local folding of the polypeptide
Tertiary structure: the unique three-dimensional structure of a
polypeptide
Quaternary structure: interactions between several polypeptides
that make up a protein
o Weak interactions between subunits help stabilize the
structure
o Nucleic Acids
constitute the genetic material of living organisms
two types
DNA
o Roles
codes for the genome of cell - entire genetic content
Genes contain instructions for producing proteins or
various forms of RNA
DNA controls all cellular activities by turning genes
on or off
RNA
o Roles
is primarily involved in protein synthesis
types include mRNA, tRNA, rRNA
K and R
K selected and r selected species
r-selected babies grow rapidly, and tend to be found in less competitive, low quality
environments. ... K-selected species produce offspring that each have a higher probability
of survival to maturity.
When considering how organisms produce energy, what are the products, reactants that we
expect to find?
Epistasis
- genes are not independent,
but rather interact to produce phenotypes
Frame shifts
Concepts associated with the amino acid table (don’t memorize each amino acid – but you
should know the concepts that I spoke about with the table)
We can make unlimited amino acids because:
o Redundant code (1 acid=4 unique codons)
o Length & sequence varies
And a few other things.
Cell theory: which states that one or more cells comprise all living things, the cell is the basic
unit of life, and new cells arise from
existing cells.