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Topic
Page:

Date: 03I D| 23

EsTMATLON r SERUM auuTAMATE BeuYATE kANSAINASEALATI

By ReITMAN AND FRANKEL MeTHÐD UstNG REANY KIT
SUPPLIED by SAN DIAGNOSTIC PT. LDT

Alarine anino teonJaase ALAT cabalyses the tans ominsbm

achen a 2- alanine and a- kelo glotaate o azmnpyxuvate
Jywate
,9 Dinitiphenyl Aydrazne e4 DNPH) to fawm a conesponding
hydoazena, brown cobused Complex In alkaline. medium
and fhis Can be mualaed Colbrimetica lly
a kelo gluaate t Lalanin uvate t L Gloamate
t24- DNPH Corsesporing hydazeme (brown
cSlousel complex)
Reitmon and trankel mehod is an endpÕint Coloimehic
mathod oy the ecimatien a enzyme achvity This puduct
Single point calibrahom Versien a ognal ne hod
a maximunm Pase a use and ConVenie nco
REAGENT COMPOI LION &
1 KeAGENT 1 Ri8
Bules alanin bith &e keto glutazate Slstate ad pH>7:4
Contains L-alanin , d- ketoglutae phsphate ufors
NORKING NaOH Rengent &Reagnt 8 shoull Be
diluted o t u olrch
Comesponds to o4 CN NaoH
to wse
 the in
tube STAN ousLDURE Seum RANGE Topic :
PkoCE SHEEERENCE Contins
& H 3(R33.
As 4 REnGeNT hhagin DNPHQ4-pesevatve
sabilizerand
theseagentsDARD', tel ALAT
KiREAGENT
ng
folauig
abs pyurate
CONTROLaml achvity SBabilize
colouwad
Sodium 4
NaoH.
4[N]
hble , weLe Ry.
standaa taken in shandaxd
TUe pyzvate,
Deagent
ancd
"SAMPLe
and andl at pesevahwe
(3mA Conloins
31cpeseAvatveand
Sample masked -
Nor in
n conmesporcs 2,4
all mal bioiteopheny
taKenace e as
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tange.i Date: Page
es
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Atcondingy test 7-56 Stalbiliser fo

l5o
TyL TyL)
 Topic:
Optcaltpical liotlled at hbes
cates 505nm 25
Sncubated distlled Anol
at "Bonk
GEm 4las SERUMReAGENTL
KeAceNTS
PptoaldensiEphical 5m/
all 29- ncubated The
the
densidlensity
dlenihy
o a Then andl
Dioitphenyl aater Content
stop
test o
y au "
(absas
faksosbane)
banca)(absosbance
absoxbana) the N Contoul?
ing tubes NaOH and
as a<
yuferenca teslbes BLANK
30 all
VIUV-Seachon hydangine 60
5O
sere
cas Stespechvely the
ul Ds STANDARD
a<eum ak
test
fa a )a alelecl 50uL 50L
ámlei Conto meassecl
amlSpec Then aLene(21-
standasdBonkhophotometer 21°ctebes
Dr
allorwedto PH) ar
3 the lo Thencooe COMTROL
Q50L
=
0:562 0818 0543
Date: Page
ith aball 20 aleling to as 50
alu mixecl
inSox min addec ul
the s. 50u 250uL PLESAM PionEER
5minutes ban be
testHhe popesly
cetes hen 50
5Dto
 DionEER
Page: 10
Date

CRCULATION &
The amaunt a Seum
m JooDml a Seum =
gtamate. pgsuvafe teucsaminase
X ConC ctanded TulL
ED standancd- oßay Blan k
0.610 O:562xI50 - O-943 XI50 = Q618/TU/L
O45

CLINTCAL STGNT ¾TCANCe &

Seum ALAT lonels ae eleuateo nval ane othor om
liver deseases assaciated ith hepatie ciashoss ALAT
present in ver colla 'n nuck Concenathon
thon in any otHhe axgans
Senum ALAT 2chiy is ncrasel ate myocazdial
inlaseton Goives disoclexin trouma , atex senal
inact, în vasíious acmalyie Condihen h vial kepabhs
asociatd ith necsis the elvaton letulde 0-50
hmes peak valhes eacking Betuen lh and ptk days
and seheing to he nomal levele in 3-5 weakes ta
nexentfal olcoveny:Tn tkoholie Aepahhs there th materete
incoeaseh voahepatitis ALAT vel& may Be saise
aithout elevated seum Biliubia in Cond ion noA
 -PIonEER
Topic: Page: |
Date

anicteic hepatiic The levels semán elevate

Sulbclinicaljaundice Hodesate incrasemay
extre hepatie cholestasis a l
Casei ooma a liver
TNTERPRETATION
The nomal sang aq seum glutomate uvate
tonsamoase s -58 nu Hesa the Sisult is 6 I810/L
Ahuc lies betseen the nevmal oange Theneae
he Saljecl is not
Dot Soffpringom 'any live
tunchendiso de