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    This document describes the procedure and clinical significance of measuring serum glutamate oxaloacetate transaminase (ASAT or AST) levels using the Reitman and Frankel colorimetric method. The method involves the transamination of aspartate and α-ketoglutarate to oxaloacetate and glutamate by AST, followed by a reaction with 2,4-dinitrophenylhydrazine to form a colored complex that can be measured colorimetrically. Elevated AST levels may indicate liver damage or injury from conditions like viral hepatitis, liver disease, myocardial infarction, or pulmonary embolism. The patient's AST level of 12.927 TU/L falls within the normal reference range of

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    info on experimental analysis of SGOT

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    This document describes the procedure and clinical significance of measuring serum glutamate oxaloacetate transaminase (ASAT or AST) levels using the Reitman and Frankel colorimetric method. The method involves the transamination of aspartate and α-ketoglutarate to oxaloacetate and glutamate by AST, followed by a reaction with 2,4-dinitrophenylhydrazine to form a colored complex that can be measured colorimetrically. Elevated AST levels may indicate liver damage or injury from conditions like viral hepatitis, liver disease, myocardial infarction, or pulmonary embolism. The patient's AST level of 12.927 TU/L falls within the normal reference range of

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    © All Rights Reserved
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    8 views6 pages

    SGOT

    Uploaded by

    suman pal
    This document describes the procedure and clinical significance of measuring serum glutamate oxaloacetate transaminase (ASAT or AST) levels using the Reitman and Frankel colorimetric method. The method involves the transamination of aspartate and α-ketoglutarate to oxaloacetate and glutamate by AST, followed by a reaction with 2,4-dinitrophenylhydrazine to form a colored complex that can be measured colorimetrically. Elevated AST levels may indicate liver damage or injury from conditions like viral hepatitis, liver disease, myocardial infarction, or pulmonary embolism. The patient's AST level of 12.927 TU/L falls within the normal reference range of

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    of 6

    -PIOREER°

    Topic: Page: 2
    Date:/0 /0| 123

    5L AIION e SERUM buTAMATE OxALbACETArE TRANSAMINBSE


    TASAT B REITMAN AND FRANkEL METHD UsTNG READY
    KZT SuPRLIED By SPAN DIAGNOSTIC Pr LTO:
    PeINCIPLE
    hepasbate amino tronsasave [AsAT Catalyses Be teansaminaten
    chen Lapsdke and A hetoqlesate t losm oxalea cekade
    and glatamae xa loace kte so Jorme & is copled with
    94-dinitophenyl hyctazine [R.4-DNPH] to conespondin
    hycvazone,a brosn colowel complex in alkaline medíum.
    and this can Bee measael calosmeticaly
    4heto-eteoate tL-aspastate Oxabacetate t L-lutamate
    Oxalo ocefate t 2,4- DNPH ’ Coaeponding hystrazpne (broancobua
    Complex)
    Reitmon andl Pronkel methÍd is an end pont coloimetic me thod
    Jax fhe oalnten a cazyme achity. Thi pnoducts a single pöntuse
    ibsahen vesrsn a'ariGinal method Jax maximum case a
    am Convenience

    1 REAGENT 1 RI
    baeud aypaskute indedng keto qlutomale shkcbate
    HeEPARALION OF kERaENIS

    NaoH
    kengont &kaagent 8 should be dilael
    Io Himes ohic Cor sesponds
    to Or [N] NaoH:

    Raagonl 1, Reaqent 2 and


    to wse
    -4 ase
    PlOnEERo
    Topic: Page 13
    Date :

    hetiglataake , Stabilig,pressaalve
    2 RERAEM LR]:
    a4-DNPH Colouoed sangend oontaias 9,4-Diritephenyl
    Budngine ,chbilzer peserakses
    8-ReAGENT 3(R9]:
    D Contins 4[N] NaDH

    4 REaGENT 4 Ru]:
    Nor king Pyawade ctandad (& meg to ly4 7ulL) T Con teins
    Saum pyxvate preSenvahhe and stabilizee
    RerERENCE RANGE &
    ASAT 0chiity 'n Tu al 37 2- Normal amge i 5-90 TU<L
    PRo CEDORE
    foar test tbes lweE daken amdmakel a "8lamke
    "Sandael Contal"amd"Sarople .n all lowc dest hles e
    eagenk, Shndavd amd Sample ou tken acconding to
    ollauing tubei
    DIOREER
    Topic :
    Page: 4
    Date

    REAGENTS BLANK STANDARD CONTROL SAMPLE


    REA GENZ 7 R50l Q50
    REAGENT4 502
    50uL
    SERUM

    Tho Contents all test tubes twere mixed pely


    60 mins: Then 50 ul
    diutlled ates amd 50 d a Seum Cas added to
    "Elonk" amd " GantonluspsctivelyAler addng a50 nl
    all

    hen Q-5 ml ay 4/o [N] Na oH as addd


    o al he Pest hubes t stop the seachon Then
    ohso bance aall -helesl tulbes ceo meas uoe
    bt NIthin 15 minutea at 505 nm UV- VIS
    Kpechophatomake and disiled coates a

    RESULTS
    Dphical lenstyabsorbene) a Blank a 0452
    Pphcal dlensi! absosbance )a/ Stondarde O-556
    O- 616
    PIonEER
    Topic
    Pago: )6
    Date :

    CAL CULATION&
    The amount a Beum glukamte Oxaleacetate tronsomina
    esentt n OCDma eum=
    CD al Soble D contbol stondocsd Tu<e
    Coa Standasd- CD a Blank
    O616 0605 O-O1 XY
    xIy = 12-924 TUL
    O556- D 459 o097

    CLINICAL ST6NIEICANCE 8
    Sem AST levels ae elesled n iral and othe
    forms liven disease assoicded nith kepahe necxosic
    AL à me dver Goeofe engyme ahil AsT achvity
    nCcases elatvely bnore dusing myacagdhal inaxchon
    Baeorehion n iver lsoadern trauma, /ter sAnal inbnton
    Yabiass, aemolyie eandhen Ta myacanclial nhrh
    ASAT uele sise 68 krs ao he onset a chext
    pan peok value sJeackad in 1-24 hos Qnol isAT lwel
    srekon to normal ange by 4yth or sth day in obsence
    nck Avexaqe eleuaten 4-5timek the

    Vale,hough 'nqekal in)orets, the level may he a


    P, I- 15 hmes a the
    -PionEERº
    Topic : Page: /G
    Date:

    ebvaled asto
    Cysto Joo tmes a the Uppes notmal
    Val hepatths and other lurer dseabe
    associabed aith kepalic necvesis Nodexate
    elovakon a AsAT -lwcls cee obsoved n extr
    bholestasis Pulmonasy embolism may cAue.
    Hhe nomal

    INTERPRE TATLON 8
    The n0malcange a seum gletmate oxabacotate
    tsoncaminase adt i 5-40 uL Hee the
    Suesultis 12:927 TU/L ushicl lies between the nonal
    range Theeloe the Culaiect Sutang
    oth myo canbial in<ascien, ven disosco,
    Condihen:
    Senal in<oien Aaemalyte
    ao23

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