KEMENTRIAN RISET, TEKNOLOGI DAN PENDIDIKAN TINGGI

UNIVERSITAS BRAWIJAYA
LABORATORIUM SENTRAL ILMU HAYATI
Jl Veteran, Malang 65145, Indonesia
Telp/Fax. +62341 559054
http://lsih.ub.ac.id Email : laboratoriumsentral@Ub.ac.id ; laboratoriumsentral@gmail.com

Chromatogram F2.1. B7 C:\GCMSsolution\Data\Project1\09122020\F2.1.B7-1qgd.qgd

TIC

17.461
5,000,000
15.905

17.302

17.693
16.0 17.0 18.0 18.5
min

Quantitative Result Table

ID# Name R.Time m/z Area Height Conc. Conc.Unit R.Index
1 Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (R)- (CAS)13.737 68.00 42585 14208 1.281 % 0
2 .ALPHA.-TERPINOLENE 15.905 41.00 62522 18976 1.881 % 0
3 (-)-Isopulegol 17.301 41.00 38518 12458 1.159 % 0
4 CITRONELLA 17.461 41.00 3162084 875068 95.156 % 0
5 (-)-Isopulegol 17.693 41.00 17354 5065 0.522 % 0

Library
<< Target >>
Line#:1 R.Time:13.735(Scan#:1948) MassPeaks:340
RawMode:Averaged 13.730-13.740(1947-1949) BasePeak:68.10(12921)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 68
80
60
93
40 39
53 79
20 107
30 136 260 280 320 337
151 181 208 230 372 391 415 447 470 495 519 543 573589 616 640 666 690 709 737 759 777

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
Hit#:1 Entry:33800 Library:Wiley9.lib
SI:93 Formula:C10H16 CAS:5989-27-5 MolWeight:136 RetIndex:0
CompName:Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (R)- (CAS) $$ D-1,8(9)-P-MENTHADIENE,(D-1-METHYL-4-ISOPROPENYLCYCLOHEXENE $$ d-Limonene $$ (+)-Limonene $$ d-(+)-
100 68
80
60 93
39
40 27 53
136
20 5565
77 107121
42 89 108

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
Hit#:2 Entry:33794 Library:Wiley9.lib
SI:92 Formula:C10H16 CAS:138-86-3 MolWeight:136 RetIndex:0
CompName:dl-Limonene $$ Cyclohexene, 1-methyl-4-(1-methylethenyl)- (CAS) $$ 1-P-MENTHA-1,8-DIENE $$ Limonene $Cinen $$ Nesol $$ Cinene $$ Limonen $$ Eulimen $$ Dipenten $$ Cajepu
100 68
80
60
93
40 39
27 53
20 77 107121136
42 5565 119

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
<< Target >>
Line#:1 R.Time:13.735(Scan#:1948) MassPeaks:340
RawMode:Averaged 13.730-13.740(1947-1949) BasePeak:68.10(12921)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 68
80
60
93
40 39
53 79
20 107
30 136 260 280 320 337
151 181 208 230 372 391 415 447 470 495 519 543 573589 616 640 666 690 709 737 759 777

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
Hit#:3 Entry:33801 Library:Wiley9.lib
SI:92 Formula:C10H16 CAS:5989-27-5 MolWeight:136 RetIndex:0
CompName:Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (R)- (CAS) $$ D-1,8(9)-P-MENTHADIENE,(D-1-METHYL-4-ISOPROPENYLCYCLOHEXENE $$ d-Limonene $$ (+)-Limonene $$ d-(+)-
100 68
80
60
93
40
27 39 53
20 79
121136
55 69
15 26 103

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
Hit#:4 Entry:33835 Library:Wiley9.lib
SI:92 Formula:C10H16 CAS:5989-54-8 MolWeight:136 RetIndex:0
CompName:l-Limonene $$ Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (S)- (CAS) $$ $ (-)-Limonene $$ p-Mentha-1,8-diene, (S)-(-)- (CAS) $$ (-)-Limonene $$ Limonene $$ 4-Isopropenyl-1-methyl-
100 68
80
60
93
40 39
27 53 79
20 107 136
15 26 55 69
103

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
Hit#:5 Entry:34252 Library:Wiley9.lib
SI:92 Formula:C10H16 CAS:0-00-0 MolWeight:136 RetIndex:0
CompName:2-methyl-4-isopropenylcyclohex-1-ene
100 68 93
80
60
39 67
40 27 53 79
20 55 107121
15 136
82 115

10 50 90 130 170 210 250 290 330 370 410 450 490 530 570 610 650 690 730 770
<< Target >>
Line#:2 R.Time:15.905(Scan#:2382) MassPeaks:412
RawMode:Averaged 15.900-15.910(2381-2383) BasePeak:41.00(17368)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 41
80 71

60 93
69
40
20
30 105121 148164 182 218 236 255 277 296 321 340 360 388 416 440456472 490 509 539 558 576 598 616 636 665 688 707 736 759 789

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:1 Entry:33854 Library:Wiley9.lib
SI:95 Formula:C10H16 CAS:586-62-9 MolWeight:136 RetIndex:0
CompName:.ALPHA.-TERPINOLENE $$ Cyclohexene, 1-methyl-4-(1-methylethylidene)- (CAS) $$ 1,4(8)-P-MENTHADIENE $$ 1-METHYLENE-4-ISOPROPYLENECYCLOHEXANE $$ Terpino
100 41 71
80
55
93
60
40 80
53
20 121
65 7785 97

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:2 Entry:57945 Library:Wiley9.lib
SI:93 Formula:C10H18O CAS:78-70-6 MolWeight:154 RetIndex:0
CompName:1,6-OCTADIEN-3-OL, 3,7-DIMETHYL- $$ 3,7-DIMETHYLOCTA-1,6-DIEN-3-OL $$ (+-)-LINALOOL $$ (1)-3,7-DIMETHYL-1,6-OCTADIEN-3-OL $$ .BETA.-LINALOOL $$ 1, 6-OC
100 71
41
80
55 93 OH
60
40 27 80
20 53 121
31 6572 84 96109 136

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:3 Entry:57896 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:22564-99-4 MolWeight:154 RetIndex:0
CompName:1,6-Octadien-3-ol, 3,7-dimethyl-, (.+-.)- (CAS) $$ 1,6-Octadien-3-ol, 3,7-dimethyl-, (.+/-.)- $$ 1,6-OCTADIEN-3-OL, 3,7-DIMETHYL- $$ 3,7-DIMETHYLOCTA-1,6-DIEN-3-OL $$ (+-)-
100 41
80
71
43
60 28
55
93
40 HO
80
20 53
32 77
65 84 96109 136

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:4 Entry:57956 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:78-70-6 MolWeight:154 RetIndex:0
CompName:Linalool $$ 1,6-Octadien-3-ol, 3,7-dimethyl- (CAS) $$ Linalol $$ .beta.-Linalool $$ Linalyl alcohol $$ 2,6-Dimethyl-2,7-octadien-6-ol $$ allo-Ocimenol $$ p-Linalool $$ LINOLOOL (D)
100 71
41
80
55 OH
60 93

40
80
20 53 121
65 7785 97 136

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:5 Entry:58546 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:78-70-6 MolWeight:154 RetIndex:0
CompName:LINALOOL L $$ 1,6-OCTADIEN-3-OL, 3,7-DIMETHYL- $$ 3,7-DIMETHYLOCTA-1,6-DIEN-3-OL $$ (+-)-LINALOOL $$ (1)-3,7-DIMETHYL-1,6-OCTADIEN-3-OL $$ .BETA.-LINA
100 71
41 93
80
55 OH
60
40 80
121
20 53
136
65 7786 97

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
<< Target >>
Line#:3 R.Time:17.300(Scan#:2661) MassPeaks:409
RawMode:Averaged 17.295-17.305(2660-2662) BasePeak:41.05(10979)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 41
80 67
55
60
71
40
93
20 111
30 136 154
181 199 216 233 258 277 306 323 356 378 395 415 440 460 481 498 518 556 585 614 639 670 697 724 745 767782 800

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:1 Entry:58211 Library:Wiley9.lib
SI:93 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:(-)-Isopulegol $$ Cyclohexanol, 5-methyl-2-(1-methylethenyl)-, [1R-(1.alpha.,2.beta.,5.alpha.)]- (CAS) $$ l-Isopulegol $$ Isopulegol $$ p-Menth-8-en-3-ol, (1R,3R,4S)-(-)- (CAS) $$ iso-P
100 41
80
69
60 55 81
43
40 53 93 OH
97 111
20 136
65 7785

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:2 Entry:58215 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:(-)-Isopulegol $$ Cyclohexanol, 5-methyl-2-(1-methylethenyl)-, [1R-(1.alpha.,2.beta.,5.alpha.)]- (CAS) $$ l-Isopulegol $$ Isopulegol $$ p-Menth-8-en-3-ol, (1R,3R,4S)-(-)- (CAS) $$ iso-P
100 41
80
60
55 69 81
40 27 43 OH
93
20 57 111 136
85 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:3 Entry:58574 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:21290-09-5 MolWeight:154 RetIndex:0
CompName:NEOISO(ISO)PULEGOL
100 41
OH
80
55 67
60
43 71 121
27 81 95
40 111
20 57 139
85

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:4 Entry:57814 Library:Wiley9.lib
SI:90 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41
69
80
60 O
55

40 27 43
95
7181
20 57 111
136 154
85

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:5 Entry:58223 Library:Wiley9.lib
SI:90 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:CYCLOHEXANOL, 5-METHYL-2-(1-METHYLETHENYL)-, [1R-(1.ALPHA.,2.BETA.,5.ALPHA.)]- $$ 2-ISOPROPENYL-5-METHYLCYCLOHEXANOL # $$ (-)-ISOPULEGOL $$ (-
100 41
80
69
55
60 27
29 56 81
40 43 95 121 OH
111
20 98 112 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
<< Target >>
Line#:4 R.Time:17.460(Scan#:2693) MassPeaks:439
RawMode:Averaged 17.455-17.465(2692-2694) BasePeak:41.05(859413)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 41 69
80
60
55
40 95
20 70
111
30 136 154 176 198213 244 262 282 309 334 357 375 394 414 434 461 483 512 535551 587 609 635 659 681 707 728 747 768 797

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:1 Entry:57823 Library:Wiley9.lib
SI:95 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41
80
69
60 O

55
40 95
43
27
20 57 71
84 111 136 154
85

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:2 Entry:57815 Library:Wiley9.lib
SI:94 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:6-OCTENAL, 3,7-DIMETHYL- $$ (R)-(+)-CITRONELLAL $$ .BETA.-CITRONELLAL $$ 2,3-DIHYDROCITRAL $$ 3,7 DIMETHYL 6 OCTENAL $$ 3,7-DIMETHYL-6-OCTEN-1-AL
100 41
80 69
60 O

40 27 55
95
20 43
81 110
24 59 136 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:3 Entry:57820 Library:Wiley9.lib
SI:94 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41 69
80
60 O
55 95
40
84 111
20 136 154
44 59 72 91

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:4 Entry:57817 Library:Wiley9.lib
SI:93 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41
80 69

60 O

40 55 95
20 84 111
44 72 136 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:5 Entry:57822 Library:Wiley9.lib
SI:93 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41
80 69

60 O

55 95
40
43
20 57 71
84 111
85 136 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
<< Target >>
Line#:5 R.Time:17.695(Scan#:2740) MassPeaks:387
RawMode:Averaged 17.690-17.700(2739-2741) BasePeak:41.05(4236)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 41
80 55
67
60
71
40 84
20 111 136
30 154 180 199 236 256 293 315 334 363 381396 414 441 470 498 515530546 572 599 622 648 667683 708 740756 782 800

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:1 Entry:58211 Library:Wiley9.lib
SI:92 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:(-)-Isopulegol $$ Cyclohexanol, 5-methyl-2-(1-methylethenyl)-, [1R-(1.alpha.,2.beta.,5.alpha.)]- (CAS) $$ l-Isopulegol $$ Isopulegol $$ p-Menth-8-en-3-ol, (1R,3R,4S)-(-)- (CAS) $$ iso-P
100 41
80
69
60 55 81
43
40 53 93 OH
97 111
20 136
65 7785

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:2 Entry:58574 Library:Wiley9.lib
SI:91 Formula:C10H18O CAS:21290-09-5 MolWeight:154 RetIndex:0
CompName:NEOISO(ISO)PULEGOL
100 41
OH
80
55 67
60
43 71 121
27 81 95
40 111
20 57 139
85

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:3 Entry:58215 Library:Wiley9.lib
SI:91 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:(-)-Isopulegol $$ Cyclohexanol, 5-methyl-2-(1-methylethenyl)-, [1R-(1.alpha.,2.beta.,5.alpha.)]- (CAS) $$ l-Isopulegol $$ Isopulegol $$ p-Menth-8-en-3-ol, (1R,3R,4S)-(-)- (CAS) $$ iso-P
100 41
80
60
55 69 81
40 27 43 OH
93
20 57 111 136
85 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:4 Entry:57814 Library:Wiley9.lib
SI:90 Formula:C10H18O CAS:106-23-0 MolWeight:154 RetIndex:0
CompName:CITRONELLA $$ 6-Octenal, 3,7-dimethyl- (CAS) $$ Citronellal $$ Rhodinal $$ .beta.-Citronellal $$ 3,7-Dimethyl-6-octenal $$ 2,3-Dihydrocitral $$ 3,7 DIMETHYL 6 OCTENAL $$ Lev
100 41
69
80
60 O
55

40 27 43
95
7181
20 57 111
136 154
85

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780
Hit#:5 Entry:58223 Library:Wiley9.lib
SI:90 Formula:C10H18O CAS:89-79-2 MolWeight:154 RetIndex:0
CompName:CYCLOHEXANOL, 5-METHYL-2-(1-METHYLETHENYL)-, [1R-(1.ALPHA.,2.BETA.,5.ALPHA.)]- $$ 2-ISOPROPENYL-5-METHYLCYCLOHEXANOL # $$ (-)-ISOPULEGOL $$ (-
100 41
80
69
55
60 27
29 56 81
40 43 95 121 OH
111
20 98 112 154

20 60 100 140 180 220 260 300 340 380 420 460 500 540 580 620 660 700 740 780

Method

[Comment]

===== Analytical Line 1 =====

[AOC-20i+s]
# of Rinses with Presolvent :2
# of Rinses with Solvent(post) :10
# of Rinses with Sample :2
Plunger Speed(Suction) :High
Viscosity Comp. Time :0.2 sec
Plunger Speed(Injection) :High
Syringe Insertion Speed :High
Injection Mode :Normal
Pumping Times :5
Inj. Port Dwell Time :0.3 sec
Terminal Air Gap :No
Plunger Washing Speed :High
Washing Volume :8uL
Syringe Suction Position :0.0 mm
Syringe Injection Position :0.0 mm
Solvent Selection :only A

[GC-2010]
Column Oven Temp. :40.0 °C
Injection Temp. :250.00 °C
Injection Mode :Split
Flow Control Mode :Pressure
Pressure :24.9 kPa
Total Flow :143.7 mL/min
Column Flow :0.70 mL/min
Linear Velocity :30.2 cm/sec
Purge Flow :3.0 mL/min
Split Ratio :200.0
High Pressure Injection :OFF
Carrier Gas Saver :OFF
Splitter Hold :OFF
Oven Temp. Program
Rate Temperature(°C) Hold Time(min)
- 40.0 3.00
5.00 250.0 5.00

< Ready Check Heat Unit >

Column Oven : Yes
SPL1 : Yes
MS : Yes
< Ready Check Detector(FTD/BID) >
< Ready Check Baseline Drift >
< Ready Check Injection Flow >
SPL1 Carrier : Yes
SPL1 Purge : Yes
< Ready Check APC Flow >
< Ready Check Detector APC Flow >
External Wait :No
Equilibrium Time :3.0 min

[GC Program]

[GCMS-QP2010 Ultra]
IonSourceTemp :200.00 °C
Interface Temp. :250.00 °C
Solvent Cut Time :0.50 min
Detector Gain Mode :Relative to the Tuning Result
Detector Gain :1.04 kV +0.00 kV
Threshold :0

[MS Table]
--Group 1 - Event 1--
Start Time :4.00min
End Time :50.00min
ACQ Mode :Scan
Event Time :0.30sec
Scan Speed :3333
Start m/z :30.00
End m/z :800.00

Sample Inlet Unit :GC

[MS Program]
Use MS Program :OFF