WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: BSM_SM_TUV_SQD2 Column serial Number:

Project Name: SQT_E_BSM_SM_SQD_TUV_data Sample Kit 1 Expiration date:
Sample Set Method: 070 Wavelength Accuracy SSM Sample Kit 1 Lot Number:
Processing Method Wavelength Accuracy PM Sample Kit 2 Expiration Date:
Report Method Name: Wavelength Accuracy RM Sample Kit 2 Lot Number:
User Name: System Build Version: Empower 2 Software Build 2154 SPs
Sample Set Name: Wavelength Accuracy SSM Sample Set Id: 2231 Result Set Id: 2691
Acquired By: sqte Sample Set Start Date: 12/21/2010 8:36:43 PM EST

Wavelength accuracy is determined by identifying the 2 lambda max values of caffeine.

T he spectrum of caffeine is well defined with a lambda max of 205.0 nm and 273.0 nm. Empower
measures deviations from these wavelengths to determine the accuracy of the detector. T he detector
wavelength accuracy must be within ±2 nm of each Caffeine lambda max to pass.

Wavelength Accuracy Results

Specification 1 Lambda Max Lambda Max Pass Fail

1 203nm-207nm 205 PASS

Specification 2 Lambda Max Lambda Max Pass Fail

1 271nm-275nm 273 PASS

Printed 7:46:10 PM US/Eastern 4/26/2011 Page: 1 of 4

 WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: BSM_SM_TUV_SQD2 Column serial Number:

Project Name: SQT_E_BSM_SM_SQD_TUV_data Sample Kit 1 Expiration date:
Sample Set Method: 070 Wavelength Accuracy SSM Sample Kit 1 Lot Number:
Processing Method Wavelength Accuracy PM Sample Kit 2 Expiration Date:
Report Method Name: Wavelength Accuracy RM Sample Kit 2 Lot Number:
User Name: System Build Version: Empower 2 Software Build 2154 SPs
Sample Set Name: Wavelength Accuracy SSM Sample Set Id: 2231 Result Set Id: 2691
Acquired By: sqte Sample Set Start Date: 12/21/2010 8:36:43 PM EST

Wav elength Accuracy Ov erlaid Chromatogram (Caffeine peak) 202nm - 208nm

(Ov erlaid Chromatograms hav e offset X axis)
0.60

0.40
AU

0.20

0.00

Channel ACQUITY TUV ChA 202nm

Channel ACQUITY TUV ChA 203nm
Channel ACQUITY TUV ChA 204nm
Channel ACQUITY TUV ChA 205nm
Channel ACQUITY TUV ChA 206nm
Channel ACQUITY TUV ChA 207nm
Channel ACQUITY TUV ChA 208nm

Printed 7:46:10 PM US/Eastern 4/26/2011 Page: 2 of 4

 WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: BSM_SM_TUV_SQD2 Column serial Number:

Project Name: SQT_E_BSM_SM_SQD_TUV_data Sample Kit 1 Expiration date:
Sample Set Method: 070 Wavelength Accuracy SSM Sample Kit 1 Lot Number:
Processing Method Wavelength Accuracy PM Sample Kit 2 Expiration Date:
Report Method Name: Wavelength Accuracy RM Sample Kit 2 Lot Number:
User Name: System Build Version: Empower 2 Software Build 2154 SPs
Sample Set Name: Wavelength Accuracy SSM Sample Set Id: 2231 Result Set Id: 2691
Acquired By: sqte Sample Set Start Date: 12/21/2010 8:36:43 PM EST

Wav elength Accuracy Ov erlaid Chromatogram (Caffeine peak) 270nm - 276nm

(Ov erlaid Chromatograms hav e offset X axis)

0.20

0.15
AU

0.10

0.05

0.00

Channel ACQUITY TUV ChA 270nm

Channel ACQUITY TUV ChA 271nm
Channel ACQUITY TUV ChA 272nm
Channel ACQUITY TUV ChA 273nm
Channel ACQUITY TUV ChA 274nm
Channel ACQUITY TUV ChA 275nm
Channel ACQUITY TUV ChA 276nm

Printed 7:46:10 PM US/Eastern 4/26/2011 Page: 3 of 4

 WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: BSM_SM_TUV_SQD2 Column serial Number:

Project Name: SQT_E_BSM_SM_SQD_TUV_data Sample Kit 1 Expiration date:
Sample Set Method: 070 Wavelength Accuracy SSM Sample Kit 1 Lot Number:
Processing Method Wavelength Accuracy PM Sample Kit 2 Expiration Date:
Report Method Name: Wavelength Accuracy RM Sample Kit 2 Lot Number:
User Name: System Build Version: Empower 2 Software Build 2154 SPs
Sample Set Name: Wavelength Accuracy SSM Sample Set Id: 2231 Result Set Id: 2691
Acquired By: sqte Sample Set Start Date: 12/21/2010 8:36:43 PM EST

Wav elength Accuracy Results Table

Channel Name: ACQUITY TUV ChA

Channel Height Area
Injection Id Channel Name Lambda Result Id
Description (µV) (µV*sec)
1 2306 ACQUITY TUV ChA 205 ACQUITY TUV ChA 205nm 490593 681536 2697
2 2296 ACQUITY TUV ChA 204 ACQUITY TUV ChA 204nm 488449 680985 2696
3 2316 ACQUITY TUV ChA 206 ACQUITY TUV ChA 206nm 485901 674117 2698
4 2326 ACQUITY TUV ChA 207 ACQUITY TUV ChA 207nm 479495 664111 2699
5 2286 ACQUITY TUV ChA 203 ACQUITY TUV ChA 203nm 477781 666449 2695
6 2336 ACQUITY TUV ChA 208 ACQUITY TUV ChA 208nm 463068 643000 2700
7 2275 ACQUITY TUV ChA 202 ACQUITY TUV ChA 202nm 462983 648454 2694

Wav elength Accuracy Results Table

Channel Name: ACQUITY TUV ChA

Channel Height Area
Inj ection Id Channel Name Lambda Result Id
Description (µV) (µV*sec)
1 2376 ACQUITY TUV ChA 273 ACQUITY TUV ChA 273nm 188609 259861 2704
2 2366 ACQUITY TUV ChA 272 ACQUITY TUV ChA 272nm 187438 258252 2703
3 2386 ACQUITY TUV ChA 274 ACQUITY TUV ChA 274nm 186610 256734 2705
4 2356 ACQUITY TUV ChA 271 ACQUITY TUV ChA 271nm 186521 256953 2702
5 2396 ACQUITY TUV ChA 275 ACQUITY TUV ChA 275nm 184235 254017 2706
6 2346 ACQUITY TUV ChA 270 ACQUITY TUV ChA 270nm 183115 252306 2701
7 2406 ACQUITY TUV ChA 276 ACQUITY TUV ChA 276nm 181145 248991 2707

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