WAVELENGTH ACCURACY REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 030 Wavelength Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Wavelength Accuracy PM Sample Kit 1 Lot Number: N070015
Report Method Name: Wavelength Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 10423 DB ID:
Result 847558692
Set Id 10437
Sample Set Name: Wavelength Accuracy Sample Set Start Date: 10/25/2010 6:13:31 PM

Wavelength accuracy is determined by identifying the 2 lambda max values of caffeine.

The spectrum of caffeine is well defined with a lambda max of 205.0 nm and 273.0 nm. Empower
measures deviations from these wavelengths to determine the accuracy of the detector. The detector
wavelength accuracy must be within ±2 nm of each Caffeine lambda max to pass.

Wavelength Accuracy Results

Specification 1 Lambda Max Lambda Max Pass Fail

1 203nm-207nm 206 PASS

Specification 2 Lambda Max Lambda Max Pass Fail

1 271nm-275nm 273 PASS

Printed 8:30:44 AM US/Eastern 11/17/2010 Page: 1 of 3

 WAVELENGTH ACCURACY REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 030 Wavelength Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Wavelength Accuracy PM Sample Kit 1 Lot Number: N070015
Report Method Name: Wavelength Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 10423 DB ID:
Result 847558692
Set Id 10437
Sample Set Name: Wavelength Accuracy Sample Set Start Date: 10/25/2010 6:13:31 PM

Wavelenght Accuracy Overlaid Chromatogram (Caffeine peak) 202nm - 208nm

(Overlaid Chromatograms have offset X axis)

1.50

1.00
AU

0.50

0.00

Channel ACQUITY TUV ChA 202nm

Channel ACQUITY TUV ChA 203nm
Channel ACQUITY TUV ChA 204nm
Channel ACQUITY TUV ChA 205nm
Channel ACQUITY TUV ChA 206nm
Channel ACQUITY TUV ChA 207nm
Channel ACQUITY TUV ChA 208nm

Wavelenght Accuracy Overlaid Chromatogram (Caffeine peak) 270nm - 276nm

(Overlaid Chromatograms have offset X axis)
0.60

0.40
AU

0.20

0.00

Channel ACQUITY TUV ChA 270nm

Channel ACQUITY TUV ChA 271nm
Channel ACQUITY TUV ChA 272nm
Channel ACQUITY TUV ChA 273nm
Channel ACQUITY TUV ChA 274nm
Channel ACQUITY TUV ChA 275nm
Channel ACQUITY TUV ChA 276nm

Printed 8:30:44 AM US/Eastern 11/17/2010 Page: 2 of 3

 WAVELENGTH ACCURACY REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 030 Wavelength Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Wavelength Accuracy PM Sample Kit 1 Lot Number: N070015
Report Method Name: Wavelength Acc Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 10423 DB ID:
Result 847558692
Set Id 10437
Sample Set Name: Wavelength Accuracy Sample Set Start Date: 10/25/2010 6:13:31 PM

Wavelength Accuracy Results Table

Channel Name: ACQUITY TUV ChA

Channel Height Area
Injection Id Channel Name Lambda Result Id
Description (µV) (µV*sec)
1 10460 ACQUITY TUV ChA 206 ACQUITY TUV ChA 206nm 1315268 2038980 10536
2 10467 ACQUITY TUV ChA 207 ACQUITY TUV ChA 207nm 1302826 2017547 10537
3 10453 ACQUITY TUV ChA 205 ACQUITY TUV ChA 205nm 1301853 2017741 10535
4 10446 ACQUITY TUV ChA 204 ACQUITY TUV ChA 204nm 1277857 1980957 10534
5 10474 ACQUITY TUV ChA 208 ACQUITY TUV ChA 208nm 1275940 1975840 10538
6 10439 ACQUITY TUV ChA 203 ACQUITY TUV ChA 203nm 1233582 1915219 10533
7 10431 ACQUITY TUV ChA 202 ACQUITY TUV ChA 202nm 1183412 1839536 10532

Wavelength Accuracy Results Table

Channel Name: ACQUITY TUV ChA

Channel Height Area
Injection Id Channel Name Lambda Result Id
Description (µV) (µV*sec)
1 10502 ACQUITY TUV ChA 273 ACQUITY TUV ChA 273nm 482870 753177 10542
2 10495 ACQUITY TUV ChA 272 ACQUITY TUV ChA 272nm 482141 747153 10541
3 10509 ACQUITY TUV ChA 274 ACQUITY TUV ChA 274nm 479950 750999 10543
4 10516 ACQUITY TUV ChA 275 ACQUITY TUV ChA 275nm 478021 747772 10544
5 10488 ACQUITY TUV ChA 271 ACQUITY TUV ChA 271nm 477691 740136 10540
6 10523 ACQUITY TUV ChA 276 ACQUITY TUV ChA 276nm 470494 736074 10545
7 10481 ACQUITY TUV ChA 270 ACQUITY TUV ChA 270nm 469784 727440 10539

Printed 8:30:44 AM US/Eastern 11/17/2010 Page: 3 of 3