INJECTOR LINEARITY & ACCURACY

ACQUISITION INFORMATION

System Name: ACQ_FLR_N Acquired By : Thorsonk

Project Name: SQT_ACQUITY_FLR Column Serial Number : 04052007
Sample Set Method: 070 Injector Lin Acc Sample Kit 1 Expiration Date: 5/31/2007
Processing Method Injector Linearity Acc PM Sample Kit 1 Lot Number: E07482K
Report Method Name: Injector Lin Acc Report Build Version: Empower Software Build 1154
User Name: Karen Thorson (Thorsonk) Sample Set Id: 32605 Result Set Id : 32619
Sample Set Name : Injector Lin Acc Sample Set Start Date : 4/9/2007 12:29:15 PM

Injector linearity verifies that the injection volume delivered is linear within specified criteria . Injector linearity
is determined by injecting four different injection volumes of one concentration from the same vial . Empower
plots the area versus the µL injected to determine both the injector linearity and accuracy .

The coefficient of determination (R^2) is used to measure injector linearity . The injector passes the linearity
test if R^2 > or = 0.9990.

The injector accuracy is obtained by dividing the y -intercept by the slope . Accuracy passes if the result is
within the specification of < or = 0.2 µL.

Anthracene Injector Linearity & Accuracy Results Table

Linearity R ^2 Linearity R^2 Injector Accuracy Accuracy

Linearity R^2 Accuracy
Specification Pass Fail Specification Pass Fail

1 R^2 > or = 0.9990 1.0000 PASS < or = 0.6 ul 0.2 PASS

Printed 10:02:12 AM 4/12/2007 Page: 1 of 3

 INJECTOR LINEARITY & ACCURACY

ACQUISITION INFORMATION

System Name: ACQ_FLR_N Acquired By : Thorsonk

Project Name: SQT_ACQUITY_FLR Column Serial Number : 04052007
Sample Set Method: 070 Injector Lin Acc Sample Kit 1 Expiration Date: 5/31/2007
Processing Method Injector Linearity Acc PM Sample Kit 1 Lot Number: E07482K
Report Method Name: Injector Lin Acc Report Build Version: Empower Software Build 1154
User Name: Karen Thorson (Thorsonk) Sample Set Id: 32605 Result Set Id : 32619
Sample Set Name : Injector Lin Acc Sample Set Start Date : 4/9/2007 12:29:15 PM

Overlay of Anthracene Peak Chromatograms

50.00

40.00

30.00
EU

20.00

10.00

0.00

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00
Minutes

Injector Result Table

Peak : anthracene
Injection Volume
SampleName Area Level Channel Id Result Id
(ul)
1 Anthracene 2 uL 2.00 413028 1 32625 32647
2 Anthracene 3 uL 3.00 636939 2 32631 32648
3 Anthracene 5 uL 5.00 1097687 3 32637 32649
4 Anthracene 6 uL 6.00 1331446 4 32643 32650

Printed 10:02:12 AM 4/12/2007 Page: 2 of 3

 INJECTOR LINEARITY & ACCURACY

ACQUISITION INFORMATION

System Name: ACQ_FLR_N Acquired By : Thorsonk

Project Name: SQT_ACQUITY_FLR Column Serial Number : 04052007
Sample Set Method: 070 Injector Lin Acc Sample Kit 1 Expiration Date: 5/31/2007
Processing Method Injector Linearity Acc PM Sample Kit 1 Lot Number: E07482K
Report Method Name: Injector Lin Acc Report Build Version: Empower Software Build 1154
User Name: Karen Thorson (Thorsonk) Sample Set Id: 32605 Result Set Id : 32619
Sample Set Name : Injector Lin Acc Sample Set Start Date : 4/9/2007 12:29:15 PM

I n j e c tor L i n e a r i t y R e s u l t s

Calibration Id 32645 Y Intercept -49258.363871

Equation Y = 2.30e+005 X - 4.93e+004 Slope 229758.355127

Fit Type Linear (1st Order) R^2 1.0000

Anthracene Injector Linearity Plot

1.5x106

1.0x106
Area

5.0x105

0.0

0.00 1.00 2.00 3.00 4.00 5.00 6.00

R^2 1.0000
Injector Volume (ul)

Accuracy Calculation : Injector Accuracy = |Y intercept /Slope |

Y intercept = -49258 Slope = 229758

Injector Accuracy = | -49258 | / 229758 = 0.21

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